LOCUS       RC227066                8427 bp    DNA     circular  SYN 
DEFINITION  GLG1 (Myc-DDK-tagged)-Human golgi glycoprotein 1 (GLG1), transcript variant 3
ACCESSION   NM_001145667
VERSION     NM_001145667.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4566 
                     /label="GLG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCGGCGTGTG GACGTGTACG GAGGATGTTC CGCTTGTCGG 
     1081 CGGCGCTGCA TCTGCTGCTG CTATTCGCGG CCGGGGCCGA GAAACTCCCC GGCCAGGGCG 
     1141 TCCACAGCCA GGGCCAGGGT CCCGGGGCCA ACTTTGTGTC CTTCGTAGGG CAGGCCGGAG 
     1201 GCGGCGGCCC GGCGGGTCAG CAGCTGCCCC AGCTGCCTCA GTCATCGCAG CTTCAGCAGC 
     1261 AACAGCAGCA GCAGCAACAG CAACAGCAGC CTCAGCCGCC GCAGCCGCCT TTCCCGGCGG 
     1321 GTGGGCCTCC GGCCCGGCGG GGAGGAGCGG GGGCTGGTGG GGGCTGGAAG CTGGCGGAGG 
     1381 AAGAGTCCTG CAGGGAGGAC GTGACCCGCG TGTGCCCTAA GCACACCTGG AGCAACAACC 
     1441 TGGCGGTGCT CGAGTGCCTG CAGGATGTGA GGGAGCCTGA AAATGAAATT TCTTCAGACT 
     1501 GCAATCATTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA TTTGAATCTG 
     1561 TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT GATGAACCGG 
     1621 TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC ACTGAGTATC 
     1681 AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC CGTTTAATCT 
     1741 GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC AGTATTCGGC 
     1801 TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG AAAGGCCTGG 
     1861 TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC AAGAAAGCCA 
     1921 TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT TTATATTTTG 
     1981 CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG GGCAGAGTGT 
     2041 ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT CGAGAAGCAC 
     2101 TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA TTGGCCAAAT 
     2161 CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG CGATCGCGTG 
     2221 AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA GGGCGACAAG 
     2281 TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG GAAGACTTTT 
     2341 CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT TGTTCCGGAT 
     2401 TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG GAGAAGGGGA 
     2461 ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT GACCCTGGTG 
     2521 CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC CAGACAGCCT 
     2581 GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA CATTTATACA 
     2641 CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT TTCATCTCCC 
     2701 GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT TCTCGTCTTT 
     2761 GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT GTGTTCTCTT 
     2821 GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA CGGGAGTGCC 
     2881 GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG GATCCTGCCC 
     2941 TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA GAGACTGGAC 
     3001 AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT AGAGATATAG 
     3061 TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG CTGATGAGAG 
     3121 CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG ATAGACTCTG 
     3181 GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC GAGAAGTGTG 
     3241 CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT TCTTACAAGT 
     3301 TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA AAGAAGGTGG 
     3361 ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA GCCAAGGAGC 
     3421 ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG ATGACGGAGG 
     3481 ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA AACTTCTGTT 
     3541 CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC AAGAAGCAGC 
     3601 TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG ATGGACCCAG 
     3661 AGCTAGACTA CACCCTCATG AGGGTCTGCA AGCAGATGAT AAAGAGGTTC TGTCCGGAAG 
     3721 CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA TTGATGGATC 
     3781 CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT TACCGCTTAA 
     3841 ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC GGTATCCTGA 
     3901 CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG AAGCTGAGAT 
     3961 ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT ATCCAGGAGT 
     4021 CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC GAGATCTCCA 
     4081 GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG GAGTGCCTCA 
     4141 AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA AACATGCTGA 
     4201 AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT GCCCTGGACA 
     4261 TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC TGTCTCATGG 
     4321 AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC CTCAATGACC 
     4381 GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC TCTGATCTTG 
     4441 CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC AGTGGGAGCA 
     4501 TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA GTGACACGAG 
     4561 AGCTCAAGGA CAGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//