LOCUS       RC227054                8466 bp    DNA     circular  SYN 
DEFINITION  GLG1 (Myc-DDK-tagged)-Human golgi glycoprotein 1 (GLG1), transcript variant 2
ACCESSION   NM_001145666
VERSION     NM_001145666.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4605 
                     /label="GLG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCGGCGTGTG GACGTGTACG GAGGATGTTC CGCTTGTCGG 
     1081 CGGCGCTGCA TCTGCTGCTG CTATTCGCGG CCGGGGCCGA GAAACTCCCC GGCCAGGGCG 
     1141 TCCACAGCCA GGGCCAGGGT CCCGGGGCCA ACTTTGTGTC CTTCGTAGGG CAGGCCGGAG 
     1201 GCGGCGGCCC GGCGGGTCAG CAGCTGCCCC AGCTGCCTCA GTCATCGCAG CTTCAGCAGC 
     1261 AACAGCAGCA GCAGCAACAG CAACAGCAGC CTCAGCCGCC GCAGCCGCCT TTCCCGGCGG 
     1321 GTGGGCCTCC GGCCCGGCGG GGAGGAGCGG GGGCTGGTGG GGGCTGGAAG CTGGCGGAGG 
     1381 AAGAGTCCTG CAGGGAGGAC GTGACCCGCG TGTGCCCTAA GCACACCTGG AGCAACAACC 
     1441 TGGCGGTGCT CGAGTGCCTG CAGGATGTGA GGGAGTTGTT GTGGAATTAT AAGCTGAACC 
     1501 TAACTACAGA TCCCAAATTT GAATCTGTGG CCAGAGAGGT TTGCAAATCT ACTATAACAG 
     1561 AGATTAAAGA ATGTGCTGAT GAACCGGTTG GAAAAGGTTA CATGGTTTCC TGCTTGGTGG 
     1621 ATCACCGAGG CAACATCACT GAGTATCAGT GTCACCAGTA CATTACCAAG ATGACGGCCA 
     1681 TCATTTTTAG TGATTACCGT TTAATCTGTG GCTTCATGGA TGACTGCAAA AATGACATCA 
     1741 ACATTCTGAA ATGTGGCAGT ATTCGGCTTG GAGAAAAGGA TGCACATTCA CAAGGTGAGG 
     1801 TGGTATCATG CTTGGAGAAA GGCCTGGTGA AAGAAGCAGA AGAAAGAGAA CCCAAGATTC 
     1861 AAGTTTCTGA ACTCTGCAAG AAAGCCATTC TCCGGGTGGC TGAGCTGTCA TCGGATGACT 
     1921 TTCACTTAGA CCGGCATTTA TATTTTGCTT GCCGAGATGA TCGGGAGCGT TTTTGTGAAA 
     1981 ATACACAAGC TGGTGAGGGC AGAGTGTATA AGTGCCTCTT TAACCATAAA TTTGAAGAAT 
     2041 CCATGAGTGA AAAGTGTCGA GAAGCACTTA CAACCCGCCA AAAGCTGATT GCCCAGGATT 
     2101 ATAAAGTCAG TTATTCATTG GCCAAATCCT GTAAAAGTGA CTTGAAGAAA TACCGGTGCA 
     2161 ATGTGGAAAA CCTTCCGCGA TCGCGTGAAG CCAGGCTCTC CTACTTGTTA ATGTGCCTGG 
     2221 AGTCAGCTGT ACACAGAGGG CGACAAGTCA GCAGTGAGTG CCAGGGGGAG ATGCTGGATT 
     2281 ACCGACGCAT GTTGATGGAA GACTTTTCTC TGAGCCCTGA GATCATCCTA AGCTGTCGGG 
     2341 GGGAGATTGA ACACCATTGT TCCGGATTAC ATCGAAAAGG GCGGACCCTA CACTGTCTGA 
     2401 TGAAAGTAGT TCGAGGGGAG AAGGGGAACC TTGGAATGAA CTGCCAGCAG GCGCTTCAAA 
     2461 CACTGATTCA GGAGACTGAC CCTGGTGCAG ATTACCGCAT TGATCGAGCT TTGAATGAAG 
     2521 CTTGTGAATC TGTAATCCAG ACAGCCTGCA AACATATAAG ATCTGGAGAC CCAATGATCT 
     2581 TGTCGTGCCT GATGGAACAT TTATACACAG AGAAGATGGT AGAAGACTGT GAACACCGTC 
     2641 TCTTAGAGCT GCAGTATTTC ATCTCCCGGG ATTGGAAGCT GGACCCTGTC CTGTACCGCA 
     2701 AGTGCCAGGG AGACGCTTCT CGTCTTTGCC ACACCCACGG TTGGAATGAG ACCAGTGAAT 
     2761 TTATGCCTCA GGGAGCTGTG TTCTCTTGTT TATACAGACA CGCCTACCGC ACTGAGGAAC 
     2821 AGGGAAGGAG GCTCTCACGG GAGTGCCGAG CTGAAGTCCA AAGGATCCTA CACCAGCGTG 
     2881 CCATGGATGT CAAGCTGGAT CCTGCCCTCC AGGATAAGTG CCTGATTGAT CTGGGAAAAT 
     2941 GGTGCAGTGA GAAAACAGAG ACTGGACAGG AGCTGGAGTG CCTTCAGGAC CATCTGGATG 
     3001 ACTTGGTGGT GGAGTGTAGA GATATAGTTG GCAACCTCAC TGAGTTAGAA TCAGAGGATA 
     3061 TTCAAATAGA AGCCTTGCTG ATGAGAGCCT GTGAGCCCAT AATTCAGAAC TTCTGCCACG 
     3121 ATGTGGCAGA TAACCAGATA GACTCTGGGG ACCTGATGGA GTGTCTGATA CAGAACAAAC 
     3181 ACCAGAAGGA CATGAACGAG AAGTGTGCCA TCGGAGTTAC CCACTTCCAG CTGGTGCAGA 
     3241 TGAAGGATTT TCGGTTTTCT TACAAGTTTA AAATGGCCTG CAAGGAGGAC GTGTTGAAGC 
     3301 TTTGCCCAAA CATAAAAAAG AAGGTGGACG TGGTGATCTG CCTGAGCACG ACCGTGCGCA 
     3361 ATGACACTCT GCAGGAAGCC AAGGAGCACA GGGTGTCCCT GAAGTGCCGC AGGCAGCTCC 
     3421 GTGTGGAGGA GCTGGAGATG ACGGAGGACA TCCGCTTGGA GCCAGATCTA TACGAAGCCT 
     3481 GCAAGAGTGA CATCAAAAAC TTCTGTTCCG CTGTGCAATA TGGCAACGCT CAGATTATCG 
     3541 AATGTCTGAA AGAAAACAAG AAGCAGCTAA GCACCCGCTG CCACCAAAAA GTATTTAAGC 
     3601 TGCAGGAGAC AGAGATGATG GACCCAGAGC TAGACTACAC CCTCATGAGG GTCTGCAAGC 
     3661 AGATGATAAA GAGGTTCTGT CCGGAAGCAG ATTCTAAAAC CATGTTGCAG TGCTTGAAGC 
     3721 AAAATAAAAA CAGTGAATTG ATGGATCCCA AATGCAAACA GATGATAACC AAGCGCCAGA 
     3781 TCACCCAGAA CACAGATTAC CGCTTAAACC CCATGTTAAG AAAAGCCTGT AAAGCTGACA 
     3841 TTCCTAAATT CTGTCACGGT ATCCTGACTA AGGCCAAGGA TGATTCAGAA TTAGAAGGAC 
     3901 AAGTCATCTC TTGCCTGAAG CTGAGATATG CTGACCAGCG CCTGTCTTCA GACTGTGAAG 
     3961 ACCAGATCCG AATCATTATC CAGGAGTCCG CCCTGGACTA CCGCCTGGAT CCTCAGCTCC 
     4021 AGCTGCACTG CTCAGACGAG ATCTCCAGTC TATGTGCTGA AGAAGCAGCA GCCCAAGAGC 
     4081 AGACAGGTCA GGTGGAGGAG TGCCTCAAGG TCAACCTGCT CAAGATCAAA ACAGAATTGT 
     4141 GTAAAAAGGA AGTGCTAAAC ATGCTGAAGG AAAGCAAAGC AGACATCTTT GTTGACCCGG 
     4201 TACTTCATAC TGCTTGTGCC CTGGACATTA AACACCACTG CGCAGCCATC ACCCCTGGCC 
     4261 GCGGGCGTCA AATGTCCTGT CTCATGGAAG CACTGGAGGA TAAGCGGGTG AGGTTACAGC 
     4321 CCGAGTGCAA AAAGCGCCTC AATGACCGGA TTGAGATGTG GAGTTACGCA GCAAAGGTGG 
     4381 CCCCAGCAGA TGGCTTCTCT GATCTTGCCA TGCAAGTAAT GACGTCTCCA TCTAAGAACT 
     4441 ACATTCTCTC TGTGATCAGT GGGAGCATCT GTATATTGTT CCTGATTGGC CTGATGTGTG 
     4501 GACGGATCAC CAAGCGAGTG ACACGAGAGC TCAAGGACAG GCTACAATAC AGGTCAGAGA 
     4561 CAATGGCTTA TAAAGGTTTA GTGTGGTCTC AGGATGTGAC AGGCAGTCCA GCCACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//