LOCUS       RC227022                9498 bp    DNA     circular  SYN 
DEFINITION  PCDH15 (Myc-DDK-tagged)-Human protocadherin-related 15 (PCDH15), transcript variant J
ACCESSION   NM_001142770
VERSION     NM_001142770.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5655..5746 
                     /label="Myc-DDK"  
     misc_feature    5776..6361 
                     /label="PolyA signal"  
     misc_feature    5833..5852 
                     /label="Restriction Enzyme"  
     misc_feature    6637..7309 
                     /label="ColE1"  
     misc_feature    7739..8533 
                     /label="Kan/Neo"  
     misc_feature    8568..8870 
                     /label="SV40 ori"  
     misc_feature    8956..9433 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5637..5643 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5637 
                     /label="PCDH15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCGACAG TTTTATCTCT GGACATGTTT AGCTTCAGGG ATCATCCTGG 
     1081 GCTCTCTCTT TGAAATCTGC TTGGGCCAGT ATGATGATGA TTGCAAACTA GCTAGGGGAG 
     1141 GACCACCAGC TACCATAGTT GCTATTGATG AAGAAAGTCG GAATGGTACA ATTCTGGTGG 
     1201 ACAACATGCT GATCAAAGGG ACTGCTGGAG GACCAGACCC CACCATAGAA CTTTCTTTAA 
     1261 AGGATAATGT GGATTACTGG GTGTTGATGG ATCCTGTTAA GCAAATGCTT TTCCTGAACA 
     1321 GCACCGGAAG AGTTCTGGAT AGAGATCCAC CGATGAACAT ACACTCCATT GTGGTGCAGG 
     1381 TCCAGTGCAT CAACAAAAAA GTGGGCACTA TTATCTACCA TGAAGTGCGA ATAGTGGTGA 
     1441 GAGACAGGAA TGACAACTCA CCCACTTTCA AGCATGAAAG CTACTATGCC ACAGTGAATG 
     1501 AGCTCACTCC AGTTGGTACC ACAATATTCA CAGGATTTTC AGGAGACAAT GGAGCTACAG 
     1561 ATATAGATGA TGGACCAAAT GGACAGATAG AGTATGTTAT TCAGTATAAT CCAGATGATC 
     1621 CGACATCCAA TGACACCTTT GAAATTCCCC TAATGTTGAC TGGAAATATA GTGTTAAGGA 
     1681 AGAGGCTCAA CTATGAAGAT AAGACTCGCT ACTTTGTCAT AATCCAAGCT AATGACCGTG 
     1741 CCCAAAATCT GAATGAGAGG CGAACCACCA CCACCACTCT CACAGTGGAT GTTCTGGATG 
     1801 GAGATGACTT GGGTCCAATG TTTCTTCCTT GTGTCCTTGT GCCAAACACT CGTGATTGCC 
     1861 GTCCACTCAC TTATCAAGCT GCCATACCTG AGTTGAGAAC TCCGGAAGAA CTGAACCCCA 
     1921 TTATTGTTAC GCCACCAATC CAAGCCATTG ATCAGGACCG GAATATTCAA CCGCCATCAG 
     1981 ATAGGCCAGG AATCCTCTAT TCCATCCTTG TTGGGACTCC TGAGGATTAC CCACGATTTT 
     2041 TCCATATGCA TCCTAGGACA GCAGAACTTA GTCTCCTGGA GCCAGTAAAC AGAGACTTTC 
     2101 ACCAGAAATT TGATTTGGTT ATTAAGGCTG AACAAGACAA TGGTCATCCT CTTCCTGCCT 
     2161 TTGCCGGTCT ACACATTGAA ATACTGGATG AAAACAATCA AAGTCCATAT TTTACAATGC 
     2221 CCAGTTATCA AGGCTATATC CTGGAATCTG CCCCAGTGGG AGCAACCATT TCGGACAGTC 
     2281 TCAATTTGAC TTCACCTTTA AGAATAGTAG CTCTGGACAA GGACATAGAA GATACAAAAG 
     2341 ACCCAGAGCT TCACCTTTTT CTGAATGACT ACACCTCAGT CTTCACCGTC ACACAGACTG 
     2401 GTATTACTCG CTACCTCACC TTACTTCAAC CAGTGGACAG GGAAGAACAG CAAACTTACA 
     2461 CCTTTTCGAT AACAGCATTT GATGGTGTAC AAGAAAGTGA GCCAGTCATC GTCAATATTC 
     2521 AAGTGATGGA TGCAAATGAT AACACGCCAA CCTTCCCTGA AATATCCTAT GATGTGTATG 
     2581 TTTATACAGA CATGAGACCT GGGGACAGTG TCATACAGCT CACTGCAGTC GACGCAGACG 
     2641 AAGGGTCAAA TGGGGAGATC ACATATGAAA TCCTTGTTGG GGCTCAGGGA GACTTCATCA 
     2701 TCAATAAAAC AACAGGGCTT ATCACCATCG CTCCAGGGGT GGAAATGATA GTCGGGCGGA 
     2761 CTTACGCACT CACGGTCCAA GCAGCGGATA ATGCTCCTCC TGCAGAGCGA AGGAACTCCA 
     2821 TCTGCACTGT GTATATTGAA GTGCTTCCAC CAAATAATCA AAGCCCTCCT CGCTTCCCAC 
     2881 AGCTGATGTA TAGCCTTGAA ATTAGTGAAG CCATGAGGGT TGGTGCTGTT TTATTAAATC 
     2941 TACAGGCAAC TGATCGAGAG GGAGACTCAA TAACATATGC CATTGAGAAT GGAGATCCTC 
     3001 AGAGAGTTTT TAATCTTTCA GAAACCACGG GGATTCTAAC CTTAGGGAAA GCACTGGACA 
     3061 GGGAAAGCAC TGATCGCTAC ATTCTGATCA TCACAGCTTC AGATGGCAGG CCAGATGGGA 
     3121 CCTCAACTGC CACAGTAAAC ATAGTGGTGA CAGATGTCAA TGACAATGCT CCAGTGTTTG 
     3181 ATCCTTATCT GCCAAGAAAT TTATCTGTGG TGGAAGAAGA AGCCAATGCC TTTGTGGGTC 
     3241 AAGTAAAAGC AACAGACCCT GATGCTGGAA TAAATGGTCA AGTGCACTAC AGTTTGGGTA 
     3301 ACTTTAATAA TCTTTTTCGT ATCACATCCA ATGGGAGCAT TTACACAGCA GTGAAGCTTA 
     3361 ACAGAGAAGT CAGGGACTAC TATGAACTTG TTGTTGTGGC AACAGATGGA GCAGTACACC 
     3421 CTCGTCATTC AACTCTAACC TTGGCCATCA AGGTTTTGGA CATTGATGAT AACAGTCCTG 
     3481 TGTTCACCAA TTCAACATAC ACTGTCCTTG TTGAAGAGAA TTTGCCAGCT GGGACTACCA 
     3541 TCCTTCAAAT AGAGGCCAAA GATGTCGACC TTGGAGCAAA TGTGTCTTAC CGGATAAGAA 
     3601 GCCCAGAAGT GAAGCACTTT TTTGCACTAC ATCCATTTAC AGGAGAACTA TCGCTTTTAA 
     3661 GGAGTTTAGA TTATGAGGCA TTTCCAGACC AAGAAGCAAG TATCACTTTT CTGGTAGAGG 
     3721 CCTTTGATAT TTATGGAACA ATGCCACCTG GTATTGCTAC TGTCACAGTG ATTGTAAAGG 
     3781 ATATGAATGA TTATCCTCCT GTCTTTAGTA AACGAATATA CAAAGGGATG GTGGCTCCGG 
     3841 ATGCAGTCAA GGGTACACCT ATCACAACAG TTTATGCTGA AGATGCAGAC CCTCCTGGAT 
     3901 TACCTGCAAG TCGTGTGAGG TATAGAGTAG ATGATGTACA GTTTCCTTAC CCTGCCAGTA 
     3961 TTTTTGAAGT GGAAGAAGAT TCTGGAAGAG TAATAACACG AGTCAATCTT AATGAAGAAC 
     4021 CTACAACAAT TTTTAAGTTG GTGGTGGTTG CTTTTGATGA TGGGGAGCCT GTGATGTCCA 
     4081 GCAGTGCCAC AGTGAAGATT CTTGTCTTAC ATCCTGGTGA GATCCCACGC TTCACACAGG 
     4141 AGGAATATAG ACCTCCTCCA GTAAGTGAAC TTGCCACCAA AGGGACCATG GTTGGTGTAA 
     4201 TTTCTGCTGC TGCCATTAAT CAAAGTATTG TGTACTCCAT TGTTTCAGGA AATGAAGAAG 
     4261 ATACATTTGG AATTAATAAC ATCACAGGTG TTATCTATGT GAATGGACCT CTGGATTATG 
     4321 AGACCAGGAC AAGCTATGTA CTTCGAGTCC AAGCTGATTC CCTGGAAGTG GTCCTTGCCA 
     4381 ATCTCCGAGT TCCTTCAAAA AGCAATACAG CTAAAGTATA CATTGAGATT CAGGATGAAA 
     4441 ATAATCATCC CCCAGTGTTT CAGAAAAAAT TCTACATCGG AGGTGTATCT GAAGATGCAA 
     4501 GAATGTTTAC TTCTGTACTC AGAGTGAAGG CTACTGATAA AGATACTGGC AATTATAGTG 
     4561 TCATGGCCTA CAGACTCATA ATACCACCAA TTAAAGAGGG AAAAGAAGGA TTTGTAGTGG 
     4621 AAACATATAC AGGGCTTATC AAAACTGCTA TGCTCTTCCA TAATATGAGG AGATCCTACT 
     4681 TCAAGTTTCA AGTTATTGCA ACTGACGACT ATGGGAAGGG ACTGAGCGGC AAAGCCGATG 
     4741 TACTCGTCTC CGTGGTCAAT CAGCTGGATA TGCAAGTCAT TGTTTCCAAT GTGCCTCCTA 
     4801 CTCTAGTGGA AAAAAAGATA GAAGATCTTA CAGAGATCTT GGATCGCTAT GTTCAGGAAC 
     4861 AAATTCCTGG TGCCAAGGTC GTAGTGGAGT CCATTGGAGC TCGCCGGCAT GGAGATGCCT 
     4921 TTTCCCTAGA AGATTACACC AAATGTGACT TGACTGTCTA TGCAATTGAC CCCCAAACCA 
     4981 ACAGAGCCAT CGATAGAAAT GAGCTTTTTA AATTTTTGGA TGGCAAACTA CTTGATATCA 
     5041 ATAAAGACTT TCAGCCGTAT TATGGGGAAG GAGGACGCAT TCTGGAGATC CGGACTCCAG 
     5101 AGGCAGTGAC CAGCATTAAA AAGAGAGGAG AAAGTCTAGG ATACACAGAA GGGGCCTTGT 
     5161 TGGCTCTGGC CTTCATCATC ATCCTCTGCT GCATTCCTGC CATCTTGGTG GTTTTGGTCA 
     5221 GCTACAGACA GTTTAAAGTA CGTCAAGCTG AGTGTACAAA GACTGCACGA ATTCAGGCCG 
     5281 CATTACCCGC GGCTAAACCA GCAGTGCCGG CTCCTGCACC AGTGGCAGCG CCCCCGCCGC 
     5341 CGCCGCCGCC TCCGCCAGGT GCGCATCTCT ATGAAGAACT TGGAGACAGC TCAATGCATA 
     5401 AGAATTATCC ATGGAGTCTG GAATTGATCC TGGCCAGGAA TATGGACAAG ATTATTACAG 
     5461 TTATGAGCAT GGGTATGAAA TGCCTCAATA TGGGAGTCGC CGTCGATTGT TACCACCAGC 
     5521 TGGACAGGAG GAATATGGTG AGGTGGTTGG TGAAGCTGAG GAAGAATATG AGGAGGAAGA 
     5581 GGAAGAGCCA AAGAAAATTA AAAAACCAAA GGTTGAAATT AGAGAGCCTA GTGAGGAGGA 
     5641 AGAAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5701 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5761 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5821 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5881 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5941 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     6001 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     6061 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6121 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6181 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6241 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6301 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6361 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6421 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6481 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6721 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     7021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     7081 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7141 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7201 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7261 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7321 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7381 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7441 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7501 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7561 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7621 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7681 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7741 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7801 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7861 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7921 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7981 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     8041 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     8101 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8161 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8221 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8281 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8341 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8401 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8461 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8521 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8581 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8641 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8701 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8761 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8821 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8881 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8941 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9001 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     9061 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9121 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9181 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9241 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9301 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9361 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9421 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9481 AAATTTAACG CGAATTTT
//