LOCUS       RC227015                7545 bp    DNA     circular  SYN 
DEFINITION  TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 1
ACCESSION   NM_001143819
VERSION     NM_001143819.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3702..3793 
                     /label="Myc-DDK"  
     misc_feature    3823..4408 
                     /label="PolyA signal"  
     misc_feature    3880..3899 
                     /label="Restriction Enzyme"  
     misc_feature    4684..5356 
                     /label="ColE1"  
     misc_feature    5786..6580 
                     /label="Kan/Neo"  
     misc_feature    6615..6917 
                     /label="SV40 ori"  
     misc_feature    7003..7480 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3684..3690 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3684 
                     /label="TPCN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCTTGC TACATTGCCC AGGCTGGTCT TGAACTGCTA GGCTCAAGCA 
     1081 GTTCTCCCAC CTTGACCTCC CAAAGCGCTG AGATTACAGA AGATGCCTCT AATGGAGGAG 
     1141 TTTCTGAGCA GCACCCCTGG CCCAGTGGCT TTGAAAGGGA GCTCAAACCA GAGACTATTT 
     1201 CAAGCCCTGG ATATCATATC CTGAGGGCCA CAGGAGAAGA GAACATGGCT GTGAGTTTGG 
     1261 ATGACGACGT GCCGCTCATC CTGACCTTGG ATGAGGGTGG CAGTGCCCCA CTGGCTCCCT 
     1321 CCAACGGCCT GGGCCAAGAA GAGCTACCTA GCAAAAATGG CGGCAGCTAT GCCATCCACG 
     1381 ACTCCCAGGC CCCCAGTCTC AGCTCTGGGG GTGAGAGTTC CCCCTCCAGC CCCGCACACA 
     1441 ACTGGGAGAT GAATTACCAA GAGGCAGCAA TCTACCTCCA GGAAGGCGAG AACAACGACA 
     1501 AGTTCTTCAC CCACCCCAAG GATGCCAAGG CGCTGGCGGC CTACCTCTTT GCACACAATC 
     1561 ACCTCTTCTA CCTGATGGAG CTGGCCACGG CCCTGCTGCT GCTGCTGCTC TCCCTGTGCG 
     1621 AGGCCCCCGC CGTCCCCGCA CTCCGGCTTG GCATCTATGT CCACGCCACC CTGGAGCTGT 
     1681 TTGCCCTGAT GGTGGTAGTG TTTGAACTCT GCATGAAGTT ACGCTGGCTG GGCCTCCACA 
     1741 CCTTCATCCG GCACAAGCGG ACCATGGTCA AGACCTCGGT GCTGGTGGTG CAGTTTGTCG 
     1801 AGGCCATCGT GGTGTTGGTA CGGCAGATGT CCCATGTGCG GGTGACCCGA GCACTGCGCT 
     1861 GCATTTTCCT GGTGGACTGT CGGTATTGCG GTGGCGTCCG GCGCAACCTG CGGCAGATCT 
     1921 TCCAGTCCCT GCCGCCCTTC ATGGACATCC TCCTGCTGCT GCTGTTCTTC ATGATCATCT 
     1981 TTGCCATCCT CGGTTTCTAC TTGTTCTCCC CTAACCCTTC AGACCCCTAC TTCAGCACCC 
     2041 TGGAGAACAG CATCGTCAGT CTGTTTGTCC TTCTGACCAC AGCCAATTTC CCAGATGTGA 
     2101 TGATGCCCTC CTACTCCCGG AACCCCTGGT CCTGCGTCTT CTTCATCGTG TACCTCTCCA 
     2161 TCGAGCTGTA TTTCATCATG AACCTGCTTC TGGCTGTGGT GTTCGACACC TTCAATGACA 
     2221 TTGAGAAACG CAAGTTCAAG TCTTTGCTAC TGCACAAGCG AACCGCTATC CAGCATGCCT 
     2281 ACCGCCTGCT CATCAGCCAG AGGAGGCCTG CCGGCATCTC CTACAGGCAG TTTGAAGGCC 
     2341 TCATGCGCTT CTACAAGCCC CGGATGAGTG CCAGGGAGCG CTATCTTACC TTCAAGGCCC 
     2401 TGAATCAGAA CAACACACCC CTGCTCAGCC TAAAGGACTT TTACGATATC TACGAAGTTG 
     2461 CTGCTTTGAA GTGGAAGGCC AAGAAAAACA GAGAGCACTG GTTTGATGAG CTTCCCAGGA 
     2521 CGGCGCTCCT CATCTTCAAA GGTATTAATA TCCTTGTGAA GTCCAAGGCC TTCCAGTATT 
     2581 TCATGTACTT GGTGGTGGCA GTCAACGGGG TCTGGATCCT CGTGGAGACA TTTATGCTGA 
     2641 AAGGTGGGAA CTTCTTCTCC AAGCACGTGC CCTGGAGTTA CCTCGTCTTT CTAACTATCT 
     2701 ATGGGGTGGA GCTGTTCCTG AAGGTTGCCG GCCTGGGCCC TGTGGAGTAC TTGTCTTCCG 
     2761 GATGGAACTT GTTTGACTTC TCCGTGACAG TGTTCGCCTT CCTGGGACTG CTGGCGCTGG 
     2821 CCCTCAACAT GGAGCCCTTC TATTTCATCG TGGTCCTGCG CCCCCTCCAG CTGCTGAGGT 
     2881 TGTTTAAGTT GAAGGAGCGC TACCGCAACG TGCTGGACAC CATGTTCGAG CTGCTGCCCC 
     2941 GGATGGCCAG CCTGGGCCTC ACCCTGCTCA TCTTTTACTA CTCCTTCGCC ATCGTGGGCA 
     3001 TGGAGTTCTT CTGCGGGATC GTCTTCCCCA ACTGCTGCAA CACGAGTACA GTGGCAGATG 
     3061 CCTACCGCTG GCGCAACCAC ACCGTGGGCA ACAGGACCGT GGTGGAGGAA GGCTACTATT 
     3121 ATCTCAATAA TTTTGACAAC ATCCTCAACA GCTTTGTGAC CCTGTTTGAG CTCACAGTTG 
     3181 TCAACAACTG GTACATCATC ATGGAAGGCG TCACCTCTCA GACCTCCCAC TGGAGCCGCC 
     3241 TCTACTTCAT GACCTTTTAC ATTGTGACCA TGGTGGTGAT GACGATCATT GTCGCCTTTA 
     3301 TCCTCGAGGC CTTCGTCTTC CGAATGAACT ACAGCCGCAA GAACCAGGAC TCGGAAGTTG 
     3361 ATGGTGGCAT CACCCTTGAG AAGGAAATCT CCAAAGAAGA GCTGGTTGCC GTCCTGGAGC 
     3421 TCTACCGGGA GGCACGGGGG GCCTCCTCGG ATGTCACCAG GCTGCTGGAG ACCCTCTCCC 
     3481 AGATGGAGAG ATACCAGCAA CATTCCATGG TGTTTCTGGG ACGGCGATCA AGGACCAAGA 
     3541 GCGACCTGAG CCTGAAGATG TACCAGGAGG AGATCCAGGA GTGGTATGAG GAGCATGCCA 
     3601 GGGAGCAAGA GCAGCAGCGA CAACTCAGCA GCAGTGCAGC CCCCGCCGCC CAGCAGCCCC 
     3661 CAGGCAGCCG CCAGCGCTCC CAGACCGTTA CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3721 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3781 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3841 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3901 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3961 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4021 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4081 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4141 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4201 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4261 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4321 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4381 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4441 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4501 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5281 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5341 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5401 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5461 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5521 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5581 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5641 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5701 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5761 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5821 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5881 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5941 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6001 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6061 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6121 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6181 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6241 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6301 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6361 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6421 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6481 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6541 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6601 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6661 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6721 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6781 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6841 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6961 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7021 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7081 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7141 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7201 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7261 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7321 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7381 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7441 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7501 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//