LOCUS       RC226849                9294 bp    DNA     circular  SYN 
DEFINITION  CLASP1 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 3
ACCESSION   NM_001142274
VERSION     NM_001142274.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5451..5542 
                     /label="Myc-DDK"  
     misc_feature    5572..6157 
                     /label="PolyA signal"  
     misc_feature    5629..5648 
                     /label="Restriction Enzyme"  
     misc_feature    6433..7105 
                     /label="ColE1"  
     misc_feature    7535..8329 
                     /label="Kan/Neo"  
     misc_feature    8364..8666 
                     /label="SV40 ori"  
     misc_feature    8752..9229 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5433..5439 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5433 
                     /label="CLASP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTCGC ATGGAGTCCT GCCTGGCGCA GGTGTTGCAG AAGGATGTGG 
     1081 GGAAACGATT GCAGGTTGGC CAAGAACTGA TAGACTATTT CTCAGACAAA CAGAAGTCTG 
     1141 CTGACCTTGA GCATGACCAG ACCATGTTAG ATAAACTTGT GGATGGACTT GCTACCTCTT 
     1201 GGGTGAACTC TAGCAATTAC AAGGTGGTTC TGCTGGGCAT GGACATCCTG TCCGCCCTGG 
     1261 TGACCCGGCT GCAGGATCGG TTCAAGGCGC AGATCGGCAC AGTGCTGCCA AGTCTAATAG 
     1321 ACAGACTAGG AGATGCTAAA GACTCTGTGA GGGAGCAGGA CCAAACTCTG CTGCTAAAGA 
     1381 TCATGGATCA AGCTGCTAAT CCCCAGTACG TATGGGACAG AATGCTTGGA GGCTTCAAAC 
     1441 ACAAGAATTT CCGTACTCGA GAAGGCATCT GTCTCTGCCT TATAGCAACA CTCAATGCCT 
     1501 CTGGAGCACA GACTTTAACA CTAAGCAAGA TTGTGCCACA TATATGCAAC TTACTTGGAG 
     1561 ATCCAAACAG CCAGGTTCGA GATGCAGCAA TAAACAGCTT AGTGGAAATT TACAGACATG 
     1621 TAGGAGAACG TGTGAGGGCA GATCTCAGTA AAAAAGGATT GCCACAGTCC CGGTTGAATG 
     1681 TAATTTTTAC AAAATTTGAT GAAGTCCAGA AATCTGGAAA CATGATACAA TCTGCAAATG 
     1741 ATAAAAATTT CGACGATGAA GATTCTGTGG ATGGTAACAG ACCTTCCTCT GCTAGTTCTA 
     1801 CATCATCCAA GGCTCCACCA AGTTCTCGGA GAAACGTTGG AATGGGAACC ACCCGCCGGC 
     1861 TTGGTTCATC CACCCTTGGA TCCAAGTCTT CAGCTGCAAA AGAAGGAGCT GGTGCTGTTG 
     1921 ATGAAGAGGA TTTTATTAAA GCATTTGATG ATGTACCTGT AGTACAGATT TATTCCAGCC 
     1981 GAGACCTTGA GGAATCCATA AACAAAATTA GGGAAATATT ATCTGATGAC AAGCATGATT 
     2041 GGGAGCAGAG AGTAAATGCT CTAAAAAAGA TTAGATCTTT ACTTTTGGCT GGTGCTGCTG 
     2101 AGTATGATAA CTTCTTTCAA CATTTGCGTC TTTTGGATGG AGCCTTTAAA CTCTCTGCTA 
     2161 AGGACCTGCG GTCTCAGGTA GTGCGGGAGG CTTGTATCAC GTTGGGGCAT CTGTCATCAG 
     2221 TTCTGGGGAA TAAGTTTGAC CATGGAGCTG AAGCCATTAT GCCAACTATC TTTAATTTAA 
     2281 TTCCAAACAG TGCCAAAATT ATGGCCACAT CTGGTGTTGT AGCTGTTAGG TTAATTATTC 
     2341 GGCACACACA CATCCCTAGG TTAATACCTG TCATAACAAG CAACTGTACC TCTAAGTCTG 
     2401 TCGCAGTTAG AAGGCGCTGT TTTGAATTTT TAGATTTGCT TTTACAAGAA TGGCAGACAC 
     2461 ATTCACTAGA ACGACACATA TCAGTATTAG CTGAAACAAT AAAGAAGGGA ATACATGATG 
     2521 CTGATTCCGA AGCAAGAATA GAAGCCAGAA AATGTTACTG GGGTTTCCAC AGTCACTTCA 
     2581 GCAGAGAAGC AGAGCACTTG TACCACACCT TGGAGTCCTC CTACCAGAAA GCCCTGCAGT 
     2641 CCCACCTGAA GAACTCAGAC AGCATAGTGT CTCTGCCTCA GTCAGACCGC TCATCTTCCA 
     2701 GCTCTCAAGA GAGTCTAAAT CGTCCGCTGT CTGCCAAAAG AAGTCCTACT GGAAGTACCA 
     2761 CATCTAGAGC TTCTACAGTT AGTACCAAAT CTGTGTCAAC GACTGGGTCC CTCCAGCGAT 
     2821 CTCGAAGTGA TATTGATGTG AACGCAGCAG CCAGTGCCAA ATCCAAAGTC TCCTCATCTT 
     2881 CGGGCACGAC GCCTTTCAGC TCTGCAGCAG CTTTGCCTCC AGGGTCATAC GCATCCTTAG 
     2941 GTCGGATCCG CACAAGACGG CAAAGCTCTG GGAGTGCCAC CAACGTCGCC TCTACACCTG 
     3001 ATAACCGGGG CCGCAGTCGC GCTAAAGTGG TTTCACAGTC CCAGCGATCC AGATCTGCTA 
     3061 ATCCTGCTGG TGCTGGCAGC CGGTCAAGTT CCCCAGGAAA ATTGTTGGGA AGTGGTTATG 
     3121 GTGGACTTAC TGGGGGCTCC TCACGAGGCC CACCTGTGAC ACCGTCTTCA GAAAAGCGAA 
     3181 GCAAGATTCC CAGGAGCCAG GGATGTAGCC GGGAAACAAG TCCAAACCGA ATAGGATTAG 
     3241 ATCGGTTTGG GCTTGGCCAG CCAGGAAGAA TACCTGGTTC TGTGAATGCC ATGAGAGTTC 
     3301 TGAGCACAAG TACAGATCTT GAAGCTGCTG TTGCTGATGC TTTGCTGTTA GGAGACTCCA 
     3361 GGAGCAAGAA GAAGCCTGTG AGGAGGAGAT ATGAGCCGTA TGGGATGTAT TCTGACGATG 
     3421 ATGCCAACAG TGATGCCTCA AGTGTTTGCT CTGAGCGCTC ATATGGCTCC AGGAATGGTG 
     3481 GCATTCCCCA TTATCTGCGG CAGACTGAGG ATGTAGCAGA AGTTCTCAAC CACTGTGCTA 
     3541 GTTCAAACTG GTCAGAAAGG AAAGAAGGGC TTCTGGGCCT GCAGAACTTA CTGAAGAGCC 
     3601 AAAGAACACT GAGTCGAGTT GAACTGAAAA GGTTGTGTGA GATCTTCACT CGGATGTTTG 
     3661 CTGACCCTCA TAGCAAGAGA GTTTTCAGTA TGTTTTTGGA GACTCTTGTG GATTTTATAA 
     3721 TAATTCATAA GGATGATTTA CAAGACTGGC TTTTTGTTCT TCTCACACAA TTACTTAAGA 
     3781 AAATGGGAGC AGATTTACTT GGATCTGTGC AAGCAAAAGT TCAAAAGGCT CTAGATGTCA 
     3841 CAAGGGACTC CTTTCCATTT GATCAACAAT TTAACATTTT GATGAGATTT ATTGTGGATC 
     3901 AAACTCAAAC TCCAAACCTC AAGGTCAAAG TTGCAATCCT GAAATACATT GAGTCTCTGG 
     3961 CCAGACAGAT GGATCCAACA GATTTTGTAA ACTCTAGTGA GACAAGGCTT GCTGTTTCTA 
     4021 GAATCATAAC CTGGACAACA GAACCAAAGA GTTCAGACGT GAGAAAGGCA GCACAGATTG 
     4081 TGCTAATCTC TCTGTTTGAA TTGAATACTC CTGAATTTAC CATGTTACTT GGTGCCTTGC 
     4141 CAAAAACATT CCAGGATGGT GCCACCAAAC TCCTGCACAA CCACCTCAAG AATTCCAGTA 
     4201 ACACCAGTGT GGGCTCTCCA AGCAATACGA TTGGCCGGAC GCCCTCCCGA CACACCAGCA 
     4261 GCAGGACCAG CCCCCTGACC TCACCCACCA ACTGTTCCCA TGGGGGTCTG TCTCCAAGCA 
     4321 TGCTGGACTA TGATACAGAG AACCTGAACT CTGAAGAAAT CTATAGTTCT CTACGTGGAG 
     4381 TTACAGAAGC CATTGAAAAG TTTAGTTTTC GAAGCCAAGA AGATCTGAAT GAGCCAATTA 
     4441 AACGAGATGG CAAAAAGGAG TGTGATATTG TGTCCCGCGA TGGGGGCGCT GCCTCCCCTG 
     4501 CCACTGAGGG CCGGGGGGGT AGTGAAGTAG AAGGAGGCCG GACAGCTCTG GATAACAAGA 
     4561 CCTCACTACT CAACACCCAG CCTCCGCGCG CCTTCCCGGG GCCGCGGGCG CGAGACTACA 
     4621 ACCCGTACCC CTACTCAGAT GCCATCAACA CCTACGACAA GACCGCCCTG AAAGAGGCTG 
     4681 TGTTCGATGA CGACATGGAG CAGCTTCGAG ACGTGCCCAT CGACCATTCT GACCTGGTGG 
     4741 CTGACCTTCT GAAAGAGCTG TCCAACCACA ATGAGCGAGT GGAGGAACGG AAGGGAGCCC 
     4801 TGCTGGAGCT GCTCAAGATC ACGCGGGAAG ACAGCCTTGG TGTCTGGGAG GAGCACTTCA 
     4861 AGACCATTCT GCTCCTGCTG CTGGAGACCC TTGGAGACAA AGACCATTCA ATTCGAGCAC 
     4921 TGGCGTTAAG AGTTTTGAGG GAAATTCTGA GAAATCAACC AGCAAGATTT AAAAACTACG 
     4981 CCGAGCTGAC GATTATGAAG ACTCTGGAAG CCCACAAAGA CTCCCATAAG GAGGTGGTGA 
     5041 GAGCGGCTGA GGAGGCTGCG TCCACACTGG CCAGTTCCAT CCACCCGGAG CAGTGCATCA 
     5101 AGGTGCTCTG CCCCATCATC CAGACGGCCG ACTACCCCAT CAACCTTGCT GCCATCAAGA 
     5161 TGCAGACCAA AGTCGTCGAG AGGATCGCAA AGGAGTCATT GCTGCAGCTC CTTGTCGACA 
     5221 TCATCCCAGG CTTGCTGCAG GGTTATGACA ACACCGAAAG TAGTGTGCGT AAGGCCAGCG 
     5281 TGTTTTGCTT AGTGGCAATT TATTCCGTAA TCGGAGAAGA CCTGAAACCT CACCTTGCAC 
     5341 AGCTCACAGG GAGCAAGATG AAGCTACTAA ACTTATACAT AAAGAGGGCC CAGACCACCA 
     5401 ACAGCAACAG CAGCTCCTCC TCCGATGTCT CCACGCACAG CACGCGTACG CGGCCGCTCG 
     5461 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5521 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5581 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5641 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5701 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5761 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5821 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5881 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5941 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6001 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6061 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6121 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6181 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6241 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6301 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6361 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6421 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6481 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6541 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6601 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6661 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6721 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6781 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6841 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6901 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6961 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7021 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7081 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7141 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7201 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7261 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7321 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7381 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7441 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7501 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7561 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7621 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7681 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7741 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7801 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7861 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7921 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7981 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8041 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8101 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8161 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8221 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8281 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8341 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8401 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8461 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8521 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8641 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8701 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8761 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8821 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8881 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8941 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9001 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9061 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9121 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9181 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9241 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//