LOCUS       RC226809                7137 bp    DNA     circular  SYN 
DEFINITION  FES (Myc-DDK-tagged)-Human feline sarcoma oncogene (FES), transcript variant 3
ACCESSION   NM_001143784
VERSION     NM_001143784.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3294..3385 
                     /label="Myc-DDK"  
     misc_feature    3415..4000 
                     /label="PolyA signal"  
     misc_feature    3472..3491 
                     /label="Restriction Enzyme"  
     misc_feature    4276..4948 
                     /label="ColE1"  
     misc_feature    5378..6172 
                     /label="Kan/Neo"  
     misc_feature    6207..6509 
                     /label="SV40 ori"  
     misc_feature    6595..7072 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3276..3282 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3276 
                     /label="FES"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTTCTCT TCCGAGCTGT GCAGCCCCCA GGGCCACGGG GTCCTGCAGC 
     1081 AAATGCAGGA GGCCGAGCTT CGTCTACTGG AGGGCATGAG AAAGTGGATG GCCCAGCGGG 
     1141 TCAAGAGTGA CAGGGAGTAT GCAGGACTGC TTCACCACAT GTCCCTGCAG GACAGTGGGG 
     1201 GCCAGAGCCG GGCCATCAGC CCTGACAGCC CCATCAGTCA GTCCTGGGCT GAGATCACCA 
     1261 GCCAAACTGA GGGCCTGAGC CGCTTGCTGC GGCAGCACGC AGAGGATCTG AACTCAGGGC 
     1321 CCCTGAGCAA GCTGAGCCTG CTCATCCGGG AACGGCAGCA GCTTCGCAAG ACCTACAGCG 
     1381 AGCAGTGGCA GCAGCTGCAG CAGGAGCTCA CCAAGACCCA CAGCCAGGAC ATTGAGAAGC 
     1441 TGAAGAGCCA GTACCGAGCT CTGGCACGGG ACAGTGCCCA AGCCAAGCGC AAGTACCAGG 
     1501 AGGCCAGCAA AGACAAGGAC CGTGACAAGG CTAAGGACAA GTATGTGCGC AGCCTGTGGA 
     1561 AGCTCTTTGC TCACCACAAC CGCTATGTGC TGGGCGTGCG GGCTGCGCAG CTACACCACC 
     1621 AGCACCACCA CCAGCTCCTG CTGCCCGGCC TGCTGCGGTC ACTGCAGGAC CTGCACGAGG 
     1681 AGATGGCTTG CATCCTGAAG GAGATCCTGC AGGAATACCT GGAGATTAGC AGCCTGGTGC 
     1741 AGGATGAGGT GGTGGCCATT CACCGGGAGA TGGCTGCAGC TGCTGCCCGC ATCCAGCCTG 
     1801 AGGCTGAGTA CCAAGGCTTC CTGCGACAGT ATGGGTCCGC ACCTGACGTC CCACCCTGTG 
     1861 TCACGTTCGA TGAGTCACTG CTTGAGGAGG GTGAACCGCT GGAGCCTGGG GAGCTCCAGC 
     1921 TGAACGAGCT GACTGTGGAG AGCGTGCAGC ACACGCTGAC CTCAGTGACA GATGAGCTGG 
     1981 CTGTGGCCAC CGAGATGGTG TTCAGGCGGC AGGAGATGGT TACGCAGCTG CAACAGGAGC 
     2041 TCCGGAATGA AGAGGAGAAC ACCCACCCCC GGGAGCGGGT GCAGCTGCTG GGCAAGAGGC 
     2101 AAGTGCTGCA AGAAGCACTG CAGGGGCTGC AGGTAGCGCT GTGCAGCCAG GCCAAGCTGC 
     2161 AGGCCCAGCA GGAGTTGCTG CAGACCAAGC TGGAGCACCT GGGCCCCGGC GAGCCCCCGC 
     2221 CTGTGCTGCT CCTGCAGGAT GACCGCCACT CCACGTCGTC CTCGGAGCAG GAGCGAGAGG 
     2281 GGGGAAGGAC ACCCACGCTG GAGATCCTTA AGAGCCACAT CTCAGGAATC TTCCGCCCCA 
     2341 AGTTCTCGAA CCTGTACCGA CTGGAAGGGG AAGGCTTTCC TAGCATTCCT TTGCTCATCG 
     2401 ACCACCTACT GAGCACCCAG CAGCCCCTCA CCAAGAAGAG TGGTGTTGTC CTGCACAGGG 
     2461 CTGTGCCCAA GGACAAGTGG GTGCTGAACC ATGAGGACCT GGTGTTGGGT GAGCAGATTG 
     2521 GACGGGGGAA CTTTGGCGAA GTGTTCAGCG GACGCCTGCG AGCCGACAAC ACCCTGGTGG 
     2581 CGGTGAAGTC TTGTCGAGAG ACGCTCCCAC CTGACCTCAA GGCCAAGTTT CTACAGGAAG 
     2641 CGAGGATCCT GAAGCAGTAC AGCCACCCCA ACATCGTGCG TCTCATTGGT GTCTGCACCC 
     2701 AGAAGCAGCC CATCTACATC GTCATGGAGC TTGTGCAGGG GGGCGACTTC CTGACCTTCC 
     2761 TCCGCACGGA GGGGGCCCGC CTGCGGGTGA AGACTCTGCT GCAGATGGTG GGGGATGCAG 
     2821 CTGCTGGCAT GGAGTACCTG GAGAGCAAGT GCTGCATCCA CCGGGACCTG GCTGCTCGGA 
     2881 ACTGCCTGGT GACAGAGAAG AATGTCCTGA AGATCAGTGA CTTTGGGATG TCCCGAGAGG 
     2941 AAGCCGATGG GGTCTATGCA GCCTCAGGGG GCCTCAGACA AGTCCCCGTG AAGTGGACCG 
     3001 CACCTGAGGC CCTTAACTAC GGCCGCTACT CCTCCGAAAG CGACGTGTGG AGCTTTGGCA 
     3061 TCTTGCTCTG GGAGACCTTC AGCCTGGGGG CCTCCCCCTA TCCCAACCTC AGCAATCAGC 
     3121 AGACACGGGA GTTTGTGGAG AAGGGGGGCC GTCTGCCCTG CCCAGAGCTG TGTCCTGATG 
     3181 CCGTGTTCAG GCTCATGGAG CAGTGCTGGG CCTATGAGCC TGGGCAGCGG CCCAGCTTCA 
     3241 GCACCATCTA CCAGGAGCTG CAGAGCATCC GAAAGCGGCA TCGGACGCGT ACGCGGCCGC 
     3301 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3361 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3421 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3481 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3541 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3601 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3661 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3721 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3781 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3841 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3901 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3961 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4021 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4081 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4141 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4201 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4261 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4321 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4381 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4441 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4501 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4561 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4621 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4681 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4741 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4801 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4861 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4921 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4981 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5041 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5101 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5161 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5221 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5281 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5341 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5401 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5461 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5521 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5581 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5641 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5701 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5761 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5821 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5881 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5941 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6001 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6061 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6121 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6181 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6241 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6301 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6361 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6541 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6661 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6721 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6781 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6841 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6901 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6961 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7021 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7081 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//