LOCUS       RC226781                7563 bp    DNA     circular  SYN 
DEFINITION  ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2
ACCESSION   NM_001143778
VERSION     NM_001143778.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3720..3811 
                     /label="Myc-DDK"  
     misc_feature    3841..4426 
                     /label="PolyA signal"  
     misc_feature    3898..3917 
                     /label="Restriction Enzyme"  
     misc_feature    4702..5374 
                     /label="ColE1"  
     misc_feature    5804..6598 
                     /label="Kan/Neo"  
     misc_feature    6633..6935 
                     /label="SV40 ori"  
     misc_feature    7021..7498 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3702..3708 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3702 
                     /label="ASAP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGAGCAG TTCAGCGTCG CCGAGTTCCT GGCCGTCACC GCGGAGGACC 
     1081 TCAGCTCCCC GGCTGGGGCC GCCGCCTTCG CCGCCAAGAT GCCCCGGTAC CGAGGGGCGG 
     1141 CGCTGGCGCG GGAGGAGATC TTGGAAGGAG ACCAAGCCAT CCTGCAGAGA ATAAAGAAGG 
     1201 CTGTGCGGGC AATCCATAGC TCCGGCCTTG GCCATGTGGA GAATGAAGAG CAGTACCGAG 
     1261 AGGCCGTGGA ATCCTTAGGC AACAGCCACC TGTCCCAGAA CAGCCATGAG CTGTCCACAG 
     1321 GCTTCCTAAA CTTGGCCGTG TTCACCCGCG AGGTTGCTGC GCTCTTCAAG AACCTGATTC 
     1381 AGAACTTGAA CAACATTGTC TCTTTCCCCC TGGACAGTCT GATGAAGGGG CAGCTGAGGG 
     1441 ACGGTCGACA GGCTTCTCTT TCCTTGGGGT CTAGGGCCAA GCTGGAGAAG GAGCGCGATC 
     1501 GGGCCAGGGT GACAGGAGGG ATCCCTGGGG AGGTGGCCCA GGACATGCAG AGAGAGCGGC 
     1561 GCATCTTCCA GCTGCACATG TGTGAGTATC TGCTCAAAGC CGGGGAGAGC CAGATGAAGC 
     1621 AAGGTCCTGA CTTCCTTCAG AGCCTCATCA AGTTCTTCCA CGCCCAGCAC AACTTTTTCC 
     1681 AAGATGGCTG GAAGGCTGCC CAGAGCCTGT TCCCCTTCAT CGAGAAGCTG GCGGCCTCAG 
     1741 TACATGCACT CCATCAGGCC CAGGAGGACG AGCTACAGAA GCTGACCCAG CTCCGGGACT 
     1801 CCCTCCGAGG GACACTGCAG CTTGAGAGCA GAGAGGAACA CCTGAGCCGG AAGAACTCAG 
     1861 GATGTGGCTA TAGCATCCAC CAGCACCAAG GCAACAAGCA GTTTGGGACG GAGAAAGTGG 
     1921 GCTTTCTATA CAAGAAAAGT GACGGAATTC GAAGAGTCTG GCAGAAAAGG AAGTGTGGAG 
     1981 TCAAGTATGG CTGCCTGACC ATCTCACACA GCACGATAAA CCGGCCCCCG GTGAAGCTGA 
     2041 CCCTGCTGAC GTGCCAAGTG AGGCCAAACC CTGAGGAGAA AAAGTGCTTC GACCTGGTGA 
     2101 CCCACAACCG GACGTACCAC TTTCAGGCAG AGGACGAGCA CGAGTGTGAG GCGTGGGTGT 
     2161 CAGTGTTGCA GAACAGCAAG GACGAAGCCC TGAGCAGCGC CTTCCTCGGG GAGCCCAGCG 
     2221 CTGGCCCGGG GTCCTGGGGG TCCGCCGGCC ATGATGGGGA GCCGCACGAC CTCACAAAGC 
     2281 TGCTCATCGC GGAGGTGAAG AGCAGGCCTG GGAATAGCCA GTGCTGCGAC TGCGGGGCTG 
     2341 CAGACCCCAC GTGGCTCAGC ACCAACCTGG GCGTGCTCAC CTGCATCCAG TGCTCGGGCG 
     2401 TCCACCGCGA ACTGGGCGTG CGCTTTTCGC GCATGCAGTC ACTCACCTTG GACCTGCTGG 
     2461 GCCCCTCCGA GTTGTTGCTG GCCTTGAACA TGGGAAACAC GAGCTTCAAT GAGGTCATGG 
     2521 AGGCCCAGCT ACCCTCACAC GGCGGCCCTA AACCCTCAGC TGAGAGTGAC ATGGGCACCC 
     2581 GCAGGGACTA CATTATGGCC AAGTATGTGG AGCATAGGTT TGCACGCCGG TGCACACCTG 
     2641 AGCCTCAGCG ACTCTGGACA GCCATTTGCA ACAGGGACCT CCTGTCGGTA CTGGAGGCCT 
     2701 TTGCCAATGG GCAGGACTTT GGACAGCCGC TGCCAGGGCC TGATGCACAG GCACCTGAAG 
     2761 AACTCGTCTT GCATTTGGCT GTCAAAGTCG CCAACCAGGC TTCCCTGCCT CTGGTGGATT 
     2821 TCATCATCCA GAACGGTGGT CACCTGGATG CCAAGGCTGC TGACGGGAAC ACGGCTCTGC 
     2881 ACTACGCAGC ACTCTACAAC CAGCCCGACT GCCTCAAGCT GCTGCTGAAG GGGAGAGCTT 
     2941 TGGTTGGCAC AGTAAATGAA GCAGGCGAGA CAGCTCTGGA CATAGCCAGG AAGAAGCACC 
     3001 ACAAGGAGTG TGAGGAGCTG CTGGAGCAGG CCCAGGCGGG GACCTTTGCC TTCCCTCTAC 
     3061 ATGTGGACTA CTCCTGGGTA ATTTCCACAG AGCCTGGCTC TGACAGTGAG GAGGATGAGG 
     3121 AAGAGAAGCG CTGCTTGCTG AAGCTCCCGG CCCAGGCTCA CTGGGCCAGT GGGAGGCTGG 
     3181 ACATCAGCAA CAAGACCTAT GAGACTGTCG CCAGCCTGGG AGCAGCCACC CCTCAGGGCG 
     3241 AGAGTGAGGA CTGTCCCCCG CCCTTGCCAG TCAAAAACTC TTCTCGGACT TTGGTCCAAG 
     3301 GGTGTGCAAG ACATGCCAGT GGAGATCGTT CTGAAGTCTC CAGCCTGAGT TCAGAGGCCC 
     3361 CTGAGACCCC TGAGAGCCTG GGCAGTCCAG CCTCCTCCTC CAGTCTGATG AGCCCCTTGG 
     3421 AACCTGGGGA TCCCAGCCAA GCCCCACCCA ACTCTGAAGA GGGCCTCCGA GAGCCCCCAG 
     3481 GCACCTCCAG ACCCAGCCTG ACATCCGGGA CCACCCCTTC GGAGATGTAC CTCCCCGTCA 
     3541 GATTCAGCTC CGAGAGCACT CGCTCCTATC GGCGGGGGGC GCGGAGCCCT GAAGATGGTC 
     3601 CCTCAGCCAG GCAGCCTCTG CCCAGAAGGA ACGTGCCGGT TGGCATCACT GAAGGAGATG 
     3661 GCTCAAGGAC TGGGAGTCTC CCAGCAAGTT CTGTGCAACT TTTGCAAGAC ACGCGTACGC 
     3721 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3781 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3841 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3901 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3961 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4021 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4081 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4141 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4201 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4261 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4321 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4381 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4441 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4621 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4681 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4801 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5041 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5341 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5401 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5461 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5521 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5581 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5641 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5701 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5761 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5821 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5881 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5941 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6001 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6061 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6121 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6181 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6241 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6301 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6361 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6421 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6481 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6541 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6601 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6661 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6721 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6781 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6961 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7021 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7081 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7141 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7201 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7261 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7321 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7381 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7441 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7501 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7561 TTT
//