LOCUS       RC226733                7293 bp    DNA     circular  SYN 
DEFINITION  TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant zeta
ACCESSION   NM_001135958
VERSION     NM_001135958.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3450..3541 
                     /label="Myc-DDK"  
     misc_feature    3571..4156 
                     /label="PolyA signal"  
     misc_feature    3628..3647 
                     /label="Restriction Enzyme"  
     misc_feature    4432..5104 
                     /label="ColE1"  
     misc_feature    5534..6328 
                     /label="Kan/Neo"  
     misc_feature    6363..6665 
                     /label="SV40 ori"  
     misc_feature    6751..7228 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3432..3438 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3432 
                     /label="TRPC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAGTTC TATTACAAAA GAAATGTTAA TGCTCCCTAT AGAGACCGCA 
     1081 TCCCTCTAAG GATAGTAAGA GCAGAATCAG AACTCTCGCC ATCAGAAAAA GCCTACTTGA 
     1141 ATGCTGTGGA AAAGGGAGAT TATGCCAGTG TCAAGAAATC CCTAGAGGAA GCTGAAATTT 
     1201 ATTTTAAAAT CAATATTAAT TGCATTGATC CTCTCGGAAG AACTGCTCTC CTCATTGCAA 
     1261 TTGAAAATGA GAACTTGGAG CTCATCGAAC TACTCTTAAG CTTTAATGTC TATGTTGGAG 
     1321 ATGCTCTATT ACATGCTATC AGAAAAGAAG TCGTCGGAGC TGTTGAGCTG TTATTGAACC 
     1381 ACAAAAAACC TAGTGGAGAA AAACAGTTTG TTGCCCAGCC CAATTGTCAA CAGCTGCTGG 
     1441 CATCTCGCTG GTACGATGAG TTTCCAGGCT GGAGGAGAAG ACACTGGGCA GTGAAGATGG 
     1501 TGACATGTTT CATAATAGGA CTTCTTTTTC CTGTCTTCTC TGTGTGCTAC CTGATAGCTC 
     1561 CCAAAAGCCC ACTTGGACTG TTCATCAGGA AGCCATTTAT CAAGTTTATC TGCCACACAG 
     1621 CCTCCTATTT GACTTTTTTG TTCCTGCTGC TGCTTGCCTC TCAGCACATC GACAGGTCAG 
     1681 ACTTGAACAG GCAAGGTCCA CCACCAACCA TCGTCGAGTG GATGATATTA CCGTGGGTCC 
     1741 TGGGCTTCAT ATGGGGAGAA ATTAAACAGA TGTGGGATGG CGGACTTCAG GACTACATCC 
     1801 ATGATTGGTG GAATCTAATG GACTTTGTAA TGAACTCCTT ATATTTAGCA ACAATCTCCT 
     1861 TGAAAATTGT TGCATTTGTA AAGTACAGTG CCCTTAATCC ACGAGAATCA TGGGACATGT 
     1921 GGCATCCCAC TCTGGTGGCA GAGGCTTTAT TTGCTATTGC AAACATCTTC AGTTCTCTGC 
     1981 GTCTGATCTC ACTGTTTACT GCAAATTCTC ACCTGGGACC TCTGCAAATA TCTCTGGGAA 
     2041 GAATGCTCCT GGACATTTTG AAGTTTCTAT TCATATACTG CCTTGTGTTG CTAGCATTTG 
     2101 CAAATGGCCT AAATCAATTG TACTTCTATT ATGAAGAAAC GAAAGGGTTA ACCTGCAAAG 
     2161 GCATAAGATG TGAAAAGCAG AATAATGCAT TTTCAACGTT ATTTGAGACA CTGCAGTCCC 
     2221 TGTTTTGGTC AATATTTGGG CTCATCAATT TATATGTGAC CAATGTCAAA GCACAGCATG 
     2281 AATTTACTGA GTTTGTTGGT GCCACCATGT TTGGGACATA CAATGTCATC TCTCTGGTTG 
     2341 TTCTACTCAA CATGTTAATA GCTATGATGA ATAATTCTTA CCAACTGATT GCTGACCATG 
     2401 CAGATATAGA ATGGAAATTT GCACGAACAA AGCTTTGGAT GAGTTATTTT GAAGAAGGAG 
     2461 GTACTCTGCC TACTCCCTTC AATGTCATCC CGAGCCCCAA GTCTCTCTGG TACCTGATCA 
     2521 AATGGATCTG GACACACTTG TGCAAGAAAA AGATGAGAAG AAAGCCAGAA AGTTTTGGAA 
     2581 CAATAGGGAG GCGAGCTGCT GATAACTTGA GAAGACATCA CCAATACCAA GAAGTTATGA 
     2641 GGAACCTGGT GAAGCGATAC GTTGCTGCAA TGATTAGAGA TGCTAAAACT GAAGAAGGCC 
     2701 TGACCGAAGA GAACTTTAAG GAACTAAAGC AAGACATTTC TAGTTTCCGC TTTGAAGTCC 
     2761 TGGGATTACT AAGAGGAAGC AAACTTTCCA CAATACAATC TGCGAATGCC TCGAAGGAGT 
     2821 CTTCAAATTC GGCAGACTCA GATGAAAAGA GTGATAGCGA AGGTAATAGC AAGGACAAGA 
     2881 AAAAGAATTT CAGCCTTTTT GATTTAACCA CCCTGATTCA TCCGAGATCA GCAGCAATTG 
     2941 CCTCTGAAAG ACATAACATA AGCAATGGCT CTGCCCTGGT GGTTCAGGAG CCGCCCAGGG 
     3001 AGAAGCAGAG AAAAGTGAAT TTTGTGACCG ATATCAAAAA CTTTGGGTTA TTTCATAGAC 
     3061 GATCAAAACA AAATGCTGCT GAGCAAAATG CAAACCAAAT CTTCTCTGTT TCAGAAGAAG 
     3121 TTGCTCGTCA ACAGGCTGCA GGACCACTTG AGAGAAATAT TCAACTGGAA TCTCGAGGAT 
     3181 TAGCTTCACG GGGTGACCTG AGCATTCCCG GTCTCAGTGA ACAATGTGTG TTAGTAGACC 
     3241 ATAGAGAAAG GAATACGGAC ACACTGGGGT TACAGGTAGG AAAGAGAGTG TGTCCATTCA 
     3301 AGTCAGAGAA GGTGGTGGTG GAGGACACGG TTCCTATAAT ACCAAAGGAG AAACATGCAA 
     3361 AAGAAGAGGA CTCTAGTATA GACTATGATC TAAACCTCCC AGACACAGTC ACCCACGAAG 
     3421 ATTACGTGAC CACAAGATTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3481 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3541 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3601 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3661 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3721 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3781 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3841 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3901 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3961 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4021 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4081 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4141 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4201 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4261 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4321 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4381 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4441 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4501 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4561 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4621 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4681 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4741 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4801 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4861 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4921 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4981 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5041 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5101 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5161 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5221 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5281 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5341 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5401 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5461 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5521 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5581 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5641 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5701 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5761 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5821 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5881 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5941 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6001 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6061 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6121 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6181 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6241 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6301 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6361 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6421 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6481 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6541 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6601 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6661 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6721 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6781 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6841 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6901 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6961 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7021 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7081 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7141 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7201 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7261 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//