LOCUS       RC226688                9522 bp    DNA     circular  SYN 
DEFINITION  NRXN1 (Myc-DDK-tagged)-Human neurexin 1 (NRXN1), transcript variant alpha2
ACCESSION   NM_001135659
VERSION     NM_001135659.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5679..5770 
                     /label="Myc-DDK"  
     misc_feature    5800..6385 
                     /label="PolyA signal"  
     misc_feature    5857..5876 
                     /label="Restriction Enzyme"  
     misc_feature    6661..7333 
                     /label="ColE1"  
     misc_feature    7763..8557 
                     /label="Kan/Neo"  
     misc_feature    8592..8894 
                     /label="SV40 ori"  
     misc_feature    8980..9457 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5661..5667 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5661 
                     /label="NRXN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACGGCG CTGCTCCAGC GCGGGGGCTG TTTTCTTCTG TGCCTCTCGC 
     1081 TGCTGCTCCT GGGCTGCTGG GCGGAGCTGG GCAGCGGGCT GGAGTTTCCG GGCGCCGAGG 
     1141 GCCAATGGAC GCGCTTCCCC AAGTGGAACG CCTGCTGCGA GAGCGAGATG AGCTTCCAGC 
     1201 TCAAGACTCG CAGCGCCCGC GGCCTCGTGC TCTACTTCGA CGACGAGGGC TTCTGCGACT 
     1261 TCCTGGAGCT GATTCTGACG CGCGGCGGCC GCCTGCAGCT CAGCTTCTCC ATCTTCTGCG 
     1321 CTGAGCCTGC GACGCTCCTG GCCGACACGC CGGTTAACGA CGGCGCCTGG CACAGCGTGC 
     1381 GCATCCGCCG CCAGTTCCGC AACACCACGC TCTTCATCGA CCAGGTGGAG GCCAAGTGGG 
     1441 TGGAGGTCAA GTCCAAGCGC AGGGACATGA CGGTGTTCAG CGGCCTTTTC GTCGGGGGGC 
     1501 TGCCCCCGGA ACTGCGCGCC GCGGCGCTCA AGCTCACCCT GGCCTCGGTG AGGGAGCGGG 
     1561 AGCCCTTCAA GGGGTGGATT CGTGACGTGA GGGTCAACTC CTCGCAGGTC CTGCCCGTGG 
     1621 ACAGCGGCGA GGTGAAGCTG GACGATGAGC CGCCCAACAG CGGCGGGGGA AGCCCGTGCG 
     1681 AGGCGGGCGA GGAGGGCGAG GGCGGGGTGT GCCTCAACGG AGGTGTGTGC TCCGTGGTGG 
     1741 ACGACCAGGC CGTGTGCGAC TGCTCGCGAA CCGGCTTCCG CGGCAAGGAC TGCAGCCAAG 
     1801 AAATAAAATT CGGTCTTCAG TGTGTTTTGC CGGTGCTGTT ACATGACAAT GACCAAGGAA 
     1861 AATACTGTTG TATAAATACA GCAAAGCCTC TAACAGAAAA AGACAACAAT GTGGAAGGTC 
     1921 TGGCGCACCT GATGATGGGC GACCAAGGTA AAAGTAAAGG AAAAGAAGAA TATATTGCCA 
     1981 CGTTCAAAGG ATCTGAATAC TTCTGCTACG ACTTGTCTCA AAACCCCATT CAAAGCAGCA 
     2041 GTGATGAAAT AACTCTGTCA TTTAAAACCC TTCAGAGGAA TGGACTGATG CTTCACACTG 
     2101 GGAAATCGGC TGATTATGTC AATCTTGCCC TGAAAAATGG AGCTGTCTCT CTGGTCATTA 
     2161 ATTTGGGATC AGGGGCCTTT GAAGCACTAG TGGAGCCTGT GAATGGAAAG TTTAATGATA 
     2221 ATGCCTGGCA TGATGTGAAA GTCACCAGGA ATCTGCGTCA GCACTCAGGC ATTGGACACG 
     2281 CTATGGTAAA CAAACTACAT TGTTCGGTGA CAATATCAGT GGATGGGATT CTTACCACAA 
     2341 CGGGCTACAC GCAAGAAGAT TATACCATGC TGGGGTCTGA TGACTTTTTC TATGTTGGAG 
     2401 GCAGTCCCAG CACAGCCGAC CTTCCAGGGT CACCAGTCAG TAACAACTTT ATGGGCTGTC 
     2461 TCAAAGAGGT TGTATATAAA AATAATGATG TGAGGCTGGA ATTATCTCGA CTTGCCAAGC 
     2521 AAGGAGATCC TAAGATGAAG ATCCATGGAG TGGTGGCATT TAAATGTGAG AATGTTGCAA 
     2581 CTTTAGACCC AATCACCTTT GAAACCCCAG AGTCTTTCAT CTCTTTGCCT AAATGGAATG 
     2641 CAAAGAAAAC TGGCTCCATA TCATTTGATT TCCGTACAAC AGAGCCAAAT GGCCTCATCT 
     2701 TATTTAGCCA TGGCAAGCCA AGACATCAGA AAGATGCCAA GCACCCACAG ATGATAAAGG 
     2761 TGGACTTCTT TGCTATTGAG ATGCTAGATG GCCACCTCTA CCTCCTCCTG GACATGGGGT 
     2821 CAGGTACTAT AAAAATAAAA GCCCTGTTGA AGAAAGTGAA TGATGGAGAA TGGTATCATG 
     2881 TGGACTTCCA GAGAGACGGA CGGTCAGGTA CCATTTCTGT CAACACGTTG CGTACTCCCT 
     2941 ACACTGCTCC TGGTGAGAGT GAGATTCTGG ACCTGGATGA TGAGTTGTAC CTGGGGGGGC 
     3001 TGCCAGAAAA TAAAGCTGGC CTTGTCTTCC CCACCGAGGT GTGGACTGCT CTGCTCAACT 
     3061 ATGGCTACGT GGGCTGCATC AGGGATTTGT TCATCGATGG CCAAAGCAAA GATATCCGGC 
     3121 AAATGGCTGA AGTTCAAAGT ACTGCTGGAG TGAAGCCTTC CTGCTCAAAG GAAACAGCAA 
     3181 AACCGTGCCT TAGCAACCCT TGCAAAAACA ATGGCATGTG CAGGGATGGG TGGAACAGAT 
     3241 ATGTCTGTGA TTGTTCCGGA ACAGGCTATC TTGGCAGGTC CTGTGAGAGA GAGGCAACGG 
     3301 TTTTGAGCTA TGATGGGAGC ATGTTTATGA AAATTCAGCT CCCCGTAGTC ATGCATACGG 
     3361 AGGCTGAGGA TGTTTCCTTA CGGTTCCGAT CCCAGCGTGC ATATGGCATT CTGATGGCAA 
     3421 CCACTTCTAG AGACTCTGCT GACACCCTCC GCCTGGAGCT AGACGCAGGA CGTGTGAAAC 
     3481 TGACGGTCAA TCTAGATTGT ATCAGGATTA ACTGTAATTC CAGCAAAGGT CCCGAGACTC 
     3541 TTTTTGCTGG CTATAACCTC AATGATAACG AGTGGCACAC AGTGCGTGTA GTTCGGCGTG 
     3601 GAAAAAGTTT AAAGTTAACA GTGGATGACC AACAGGCCAT GACAGGTCAA ATGGCAGGTG 
     3661 ATCATACTAG GCTGGAGTTC CATAACATAG AGACTGGCAT CATCACAGAA CGACGGTATC 
     3721 TTTCTTCTGT CCCCTCCAAC TTCATTGGAC ACCTGCAGAG CTTGACATTT AATGGAATGG 
     3781 CATACATTGA CCTGTGTAAA AATGGCGACA TAGATTACTG TGAGCTTAAT GCCAGATTTG 
     3841 GCTTCAGGAA CATCATAGCA GATCCTGTCA CCTTCAAGAC CAAATCGAGC TATGTTGCCT 
     3901 TAGCTACCTT GCAAGCCTAC ACTTCTATGC ATCTTTTTTT CCAGTTCAAG ACAACATCCC 
     3961 TAGATGGATT AATTCTATAT AACAGTGGGG ATGGAAATGA CTTTATTGTG GTTGAATTAG 
     4021 TTAAAGGGTA CTTACATTAC GTGTTTGATT TGGGAAATGG TGCTAACCTC ATCAAAGGAA 
     4081 GCTCAAATAA ACCTCTCAAT GACAATCAGT GGCACAACGT GATGATATCA AGGGACACCA 
     4141 GCAACCTCCA CACTGTAAAG ATTGACACAA AAATCACAAC GCAAATCACC GCCGGAGCCA 
     4201 GGAACTTAGA CCTCAAGAGT GACTTATATA TAGGAGGAGT AGCTAAAGAA ACATACAAAT 
     4261 CCTTACCAAA ACTTGTACAT GCCAAAGAAG GCTTTCAAGG CTGCCTGGCA TCAGTTGATT 
     4321 TAAATGGACG GCTTCCGGAC CTCATCTCCG ATGCTCTTTT CTGCAACGGA CAGATCGAGA 
     4381 GAGGATGTGA AGGGCCCAGC ACAACCTGCC AAGAGGACTC ATGTTCCAAT CAAGGTGTGT 
     4441 GCTTGCAACA ATGGGATGGC TTCAGCTGTG ACTGTAGTAT GACTTCCTTC AGTGGACCAC 
     4501 TCTGCAATGA CCCTGGGACG ACATATATCT TTAGCAAAGG TGGTGGACAA ATCACGTATA 
     4561 AGTGGCCTCC TAATGACCGA CCCAGTACAC GAGCAGACAG ACTGGCCATA GGTTTTAGCA 
     4621 CTGTTCAGAA AGAAGCCGTA TTGGTGCGAG TGGACAGTTC TTCAGGCTTG GGTGACTACC 
     4681 TAGAACTGCA TATACACCAG GGAAAAATTG GAGTTAAGTT TAATGTTGGG ACAGATGACA 
     4741 TCGCCATTGA AGAATCCAAT GCAATCATTA ATGATGGGAA ATACCATGTA GTTCGTTTCA 
     4801 CGAGGAGTGG TGGCAATGCC ACGTTGCAGG TGGACAGCTG GCCAGTGATC GAGCGCTACC 
     4861 CTGCAGGAAA CAATGATAAC GAGCGCCTGG CGATTGCTAG ACAGCGAATT CCATATCGAC 
     4921 TTGGTCGAGT AGTTGATGAA TGGCTACTCG ACAAAGGGCG TCAGCTCACA ATCTTCAATA 
     4981 GCCAAGCAAC CATAATAATT GGCGGGAAAG AGCAGGGCCA GCCCTTCCAG GGCCAGCTCT 
     5041 CTGGGCTGTA CTACAATGGC TTGAAAGTTC TGAATATGGC AGCCGAAAAC GATGCCAACA 
     5101 TCGCCATAGT GGGAAATGTG AGACTGGTTG GTGAAGTGCC TTCCTCTATG ACAACTGAGT 
     5161 CAACAGCCAC TGCCATGCAA TCAGAGATGT CCACATCAAT TATGGAGACT ACCACGACCC 
     5221 TGGCTACTAG CACAGCCAGA AGAGGAAAGC CCCCGACAAA AGAACCCATT AGCCAGACCA 
     5281 CAGATGACAT CCTTGTGGCC TCAGCAGAGT GTCCCAGCGA TGATGAGGAC ATTGACCCCT 
     5341 GTGAGCCGAG CTCAGGTGGG TTAGCCAACC CAACCCGAGC AGGCGGCAGA GAGCCGTATC 
     5401 CAGGCTCAGC AGAAGTGATC CGGGAGTCCA GCAGCACCAC GGGTATGGTC GTTGGGATAG 
     5461 TAGCCGCTGC CGCCCTGTGC ATCCTTATCC TCCTCTATGC CATGTACAAG TACAGAAACC 
     5521 GGGATGAAGG CTCATACCAT GTGGACGAGA GTCGAAACTA CATCAGTAAC TCAGCACAGT 
     5581 CCAATGGGGC TGTTGTAAAG GAGAAACAAC CCAGCAGTGC GAAAAGCTCC AACAAAAATA 
     5641 AGAAAAACAA GGATAAAGAG TATTATGTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5701 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5761 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5821 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5881 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5941 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     6001 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     6061 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     6121 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6181 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6241 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6301 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6361 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6421 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6481 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6541 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6601 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6661 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6721 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6781 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6841 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6901 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6961 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     7021 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     7081 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     7141 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7201 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7261 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7321 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7381 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7441 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7501 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7561 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7621 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7681 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7741 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7801 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7861 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7921 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7981 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     8041 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     8101 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     8161 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8221 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8281 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8341 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8401 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8461 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8521 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8581 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8641 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8701 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8761 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8821 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8881 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8941 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9001 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     9061 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     9121 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9181 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9241 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9301 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9361 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9421 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9481 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//