LOCUS       RC226681                8526 bp    DNA     circular  SYN 
DEFINITION  VWA5B1 (Myc-DDK-tagged)-Human von Willebrand factor A domain containing 5B1 (VWA5B1)
ACCESSION   NM_001039500
VERSION     NM_001039500.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="VWA5B1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGCTTG CTGAATTGGA TCACGGGGGC AGCCCTGCCC CTCACCGCGT 
     1081 CTGATGTTAC CTCCTGTGTC AGCGGTTATG CCCTGGGCCT AACTGCCTCC CTCACCTATG 
     1141 GCAACCTGGA AGCCCAGCCC TTCCAGGGCC TCTTCGTGTA CCCCCTGGAT GAGTGCACCA 
     1201 CGGTGATCGG CTTTGAGGCA GTCATTGCCG ACCGTGTCGT GACAGTACAG ATCAAGGACA 
     1261 AAGCCAAGCT GGAGAGCGGC CACTTCGATG CCTCCCATGT TCGATCCCCA ACAGTCACAG 
     1321 GGAACATTCT GCAAGACGGG GTTTCCATAG CCCCTCATTC CTGCACACCG GGAAAGGTGA 
     1381 CCTTGGACGA GGATTTGGAG CGGATCCTGT TCGTGGCCAA CCTGGGGACC ATTGCCCCCA 
     1441 TGGAGAATGT CACCATCTTC ATCAGCACCT CCTCGGAGCT CCCAACGCTG CCCAGCGGGG 
     1501 CTGTGAGGGT CCTTCTGCCT GCTGTCTGTG CCCCAACCGT GCCCCAGTTC TGCACCAAGA 
     1561 GCACTGGCAC CTCCAACCAA CAGGCCCAGG GCAAAGACAG GCACTGCTTC GGTGCCTGGG 
     1621 CCCCGGGCTC CTGGAATAAG TTGTGCCTGG CGACTCTCCT GAACACCGAA GTGTCCAACC 
     1681 CCATGGAGTA TGAGTTCAAC TTCCAGCTGG AGATCCGTGG GCCATGTCTG CTCGCAGGGG 
     1741 TGGAGAGTCC CACTCATGAG ATTCGTGCCG ACGCCGCCCC ATCTGCCCGC TCGGCCAAGA 
     1801 GCATCATCAT CACCTTGGCC AACAAGCACA CCTTTGACCG GCCTGTGGAG ATCCTCATCC 
     1861 ACCCCAGCGA GCCCCATATG CCCCATGTCC TGATAGAGAA AGGGGACATG ACCCTGGGAG 
     1921 AGTTTGACCA GCACTTGAAG GGAAGAACAG ATTTCATTAA AGGGATGAAG AAGAAGAGCA 
     1981 GAGCAGAGCG GAAGACAGAA ATCATTCGAA AACGCCTCCA CAAAGACATT CCCCACCACT 
     2041 CCGTCATCAT GCTCAACTTC TGTCCCGACC TCCAGTCAGT CCAGCCGTGC CTGAGAAAGG 
     2101 CCCACGGGGA GTTCATCTTC CTCATTGACA GGAGCAGCAG CATGAGCGGG ATCAGCATGC 
     2161 ACCGAGTCAA GGATGCCATG TTGGTGGCCC TTAAGAGCCT CATGCCAGCC TGCCTCTTCA 
     2221 ATATCATTGG GTTTGGATCC ACATTTAAGA GCCTTTTTCC TTCCAGCCAG ACCTACAGTG 
     2281 AGGACAGCTT GGCCATGGCT TGTGATGACA TCCAGAGAAT GAAGGCCGAC ATGGGTGGGA 
     2341 CCAACATCCT TTCCCCTCTC AAGTGGGTCA TCAGGCAGCC AGTGCACCGA GGCCACCCGC 
     2401 GGCTCCTCTT CGTGATCACA GATGGCGCTG TCAACAACAC AGGGAAGGTG CTGGAGCTGG 
     2461 TGCGAAATCA CGCCTTCTCC ACCAGGTGCT ATAGCTTTGG AATTGGACCC AACGTCTGCC 
     2521 ACAGACTGGT GAAAGGACTG GCATCTGTGT CCGAGGGCAG TGCTGAGCTC CTGATGGAGG 
     2581 GGGAGCGGCT GCAACCCAAG ATGGTCAAAT CCTTGAAGAA GGCCATGGCC CCAGTCCTGA 
     2641 GCGATGTGAC TGTGGAGTGG ATCTTCCCTG AGACCACTGA GGTCCTGGTC TCACCCGTCA 
     2701 GCGCCAGCTC CCTCTTCCCT GGAGAACGGC TGGTGGGGTA TGGCATTGTA TGTGATGCTT 
     2761 CTTTGCACAT CTCCAATCCC AGATCTGACA AGAGGCGCCG GTACAGCATG CTGCACTCTC 
     2821 AGGAGTCTGG CAGCTCTGTC TTCTACCACT CTCAGGATGA CGGACCCGGG CTGGAAGGTG 
     2881 GAGACTGTGC CAAGAACTCG GGGGCACCCT TCATCCTAGG GCAGGCCAAA AATGCCCGGC 
     2941 TAGCCAGCGG AGACTCTACC ACCAAGCACG ATCTGAACCT CTCTCAGCGA CGGAGGGCAT 
     3001 ACAGCACCAA CCAGATCACC AATCACAAGC CCCTCCCAAG AGCCACCATG GCAAGTGACC 
     3061 CCATGCCAGC TGCCAAGAGA TACCCACTGC GGAAAGCCAG GCTGCAGGAC CTCACCAACC 
     3121 AGACCAGCCT GGATGTCCAG CGGTGGCAGA TTGATTTGCA GCCCTTGCTG AACAGTGGTC 
     3181 AGGACCTGAA CCAGGGCCCC AAACTCCGTG GCCCAGGGGC CCGAAGGCCC TCTCTGCTGC 
     3241 CCCAAGGCTG CCAGCCCTTC CTGCCCTGGG GCCAGGAGAC CCAGGCCTGG AGCCCTGTGA 
     3301 GAGAGCGGAC TTCTGACAGC CGAAGCCCTG GAGATCTGGA GCCGTCCCAC CATCCCTCTG 
     3361 CCTTCGAGAC AGAGACGTCC TCGGACTGGG ACCCCCCAGC CGAGTCCCAG GAGCGAGCCA 
     3421 GTCCCAGCAG GCCCGCCACC CCGGCCCCGG TGCTGGGCAA GGCCCTGGTC AAAGGCCTGC 
     3481 ACGACAGCCA ACGCCTGCAG TGGGAGGTGA GCTTCGAGCT GGGGACCCCT GGACCGGAGC 
     3541 GGGGCGGCGC GCAGGATGCC GACCTATGGA GCGAGACCTT CCACCACCTG GCGGCCCGCG 
     3601 CCATCATCCG CGACTTCGAG CAGCTGGCGG AGCGCGAGGG CGAGATCGAG CAGGGGTCCA 
     3661 ACCGCCGCTA CCAAGTGAGC GCCTTGCACA CCAGCAAGGC CTGCAACATC ATTAGCAAAT 
     3721 ACACAGCCTT CGTGCCTGTG GACGTGAGCA AGAGCCGGTA CCTGCCCACC GTGGTGGAGT 
     3781 ACCCCAACTC TGGAGCTGCC CTGCGTATGC TTGGCTCTCG GGCCCTGGCC CAACAGTGGA 
     3841 GGGGCACCTC TTCTGGCTTT GGAAGGCCGC AGACGATGCT TGGAGAAGAT TCGGCACCAG 
     3901 GAAATGACAT GGAGGCAAGT CCCACTGCTC TCTTCAGCGA GGCCAGGTCC CCCGGCCGCG 
     3961 AGAAGCACGG TGCTTCTGAA GGTCCCCAGC GCAGCCTGGC TACAAATACT CTTTCTTCCA 
     4021 TGAAGGCCTC AGAGAATCTC TTTGGATCCT GGCTAAATCT CAACAAGTCC AGGCTACTGA 
     4081 CGCGAGCAGC CAAGGGCTTC CTGAGCAAGC CACTGATCAA AGCTGTGGAG TCGACCTCCG 
     4141 GGAACCAGAG CTTCGACTAC ATACCTCTGG TGTCTCTGCA GCTGGCCTCC GGAGCCTTCC 
     4201 TGCTCAACGA AGCCTTCTGT GAGGCCACGC ACATCCCCAT GGAGAAGCTC AAGTGGACGT 
     4261 CCCCCTTCAC CTGCCATCGA GTGTCCCTCA CCACCCGCCC GTCTGAGTCC AAGACCCCGA 
     4321 GTCCCCAGCT GTGCACCAGC TCCCCGCCTA GGCACCCGTC CTGTGACAGC TTCTCCCTGG 
     4381 AGCCTCTGGC CAAGGGCAAG CTGGGCCTGG AGCCGAGGGC AGTGGTGGAG CACACTGGGA 
     4441 AGCTGTGGGC CACGGTGGTG GGGCTGGCAT GGCTGGAGCA CAGTTCGGCC TCCTACTTCA 
     4501 CTGAGTGGGA GTTGGTGGCT GCCAAGGCCA ACTCATGGCT GGAGCAGCAG GAAGTACCCG 
     4561 AGGGCCGCAC GCAGGGCACA CTCAAGGCCG CTGCCCGCCA GCTGTTTGTG CTTCTGCGGC 
     4621 ACTGGGATGA GAATCTCGAG TTCAATATGC TCTGCTATAA CCCGAATTAT GTGACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//