LOCUS       RC226625                9318 bp    DNA     circular  SYN 
DEFINITION  CLASP1 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 2
ACCESSION   NM_001142273
VERSION     NM_001142273.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5475..5566 
                     /label="Myc-DDK"  
     misc_feature    5596..6181 
                     /label="PolyA signal"  
     misc_feature    5653..5672 
                     /label="Restriction Enzyme"  
     misc_feature    6457..7129 
                     /label="ColE1"  
     misc_feature    7559..8353 
                     /label="Kan/Neo"  
     misc_feature    8388..8690 
                     /label="SV40 ori"  
     misc_feature    8776..9253 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5457..5463 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5457 
                     /label="CLASP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTCGC ATGGAGTCCT GCCTGGCGCA GGTGTTGCAG AAGGATGTGG 
     1081 GGAAACGATT GCAGGTTGGC CAAGAACTGA TAGACTATTT CTCAGACAAA CAGAAGTCTG 
     1141 CTGACCTTGA GCATGACCAG ACCATGTTAG ATAAACTTGT GGATGGACTT GCTACCTCTT 
     1201 GGGTGAACTC TAGCAATTAC AAGGTGGTTC TGCTGGGCAT GGACATCCTG TCCGCCCTGG 
     1261 TGACCCGGCT GCAGGATCGG TTCAAGGCGC AGATCGGCAC AGTGCTGCCA AGTCTAATAG 
     1321 ACAGACTAGG AGATGCTAAA GACTCTGTGA GGGAGCAGGA CCAAACTCTG CTGCTAAAGA 
     1381 TCATGGATCA AGCTGCTAAT CCCCAGTACG TATGGGACAG AATGCTTGGA GGCTTCAAAC 
     1441 ACAAGAATTT CCGTACTCGA GAAGGCATCT GTCTCTGCCT TATAGCAACA CTCAATGCCT 
     1501 CTGGAGCACA GACTTTAACA CTAAGCAAGA TTGTGCCACA TATATGCAAC TTACTTGGAG 
     1561 ATCCAAACAG CCAGGTTCGA GATGCAGCAA TAAACAGCTT AGTGGAAATT TACAGACATG 
     1621 TAGGAGAACG TGTGAGGGCA GATCTCAGTA AAAAAGGATT GCCACAGTCC CGGTTGAATG 
     1681 TAATTTTTAC AAAATTTGAT GAAGTCCAGA AATCTGGAAA CATGATACAA TCTGCAAATG 
     1741 ATAAAAATTT CGACGATGAA GATTCTGTGG ATGGTAACAG ACCTTCCTCT GCTAGTTCTA 
     1801 CATCATCCAA GGCTCCACCA AGTTCTCGGA GAAACGTTGG AATGGGAACC ACCCGCCGGC 
     1861 TTGGTTCATC CACCCTTGGA TCCAAGTCTT CAGCTGCAAA AGAAGGAGCT GGTGCTGTTG 
     1921 ATGAAGAGGA TTTTATTAAA GCATTTGATG ATGTACCTGT AGTACAGATT TATTCCAGCC 
     1981 GAGACCTTGA GGAATCCATA AACAAAATTA GGGAAATATT ATCTGATGAC AAGCATGATT 
     2041 GGGAGCAGAG AGTAAATGCT CTAAAAAAGA TTAGATCTTT ACTTTTGGCT GGTGCTGCTG 
     2101 AGTATGATAA CTTCTTTCAA CATTTGCGTC TTTTGGATGG AGCCTTTAAA CTCTCTGCTA 
     2161 AGGACCTGCG GTCTCAGGTA GTGCGGGAGG CTTGTATCAC GTTGGGGCAT CTGTCATCAG 
     2221 TTCTGGGGAA TAAGTTTGAC CATGGAGCTG AAGCCATTAT GCCAACTATC TTTAATTTAA 
     2281 TTCCAAACAG TGCCAAAATT ATGGCCACAT CTGGTGTTGT AGCTGTTAGG TTAATTATTC 
     2341 GGCACACACA CATCCCTAGG TTAATACCTG TCATAACAAG CAACTGTACC TCTAAGTCTG 
     2401 TCGCAGTTAG AAGGCGCTGT TTTGAATTTT TAGATTTGCT TTTACAAGAA TGGCAGACAC 
     2461 ATTCACTAGA ACGACACATA TCAGTATTAG CTGAAACAAT AAAGAAGGGA ATACATGATG 
     2521 CTGATTCCGA AGCAAGAATA GAAGCCAGAA AATGTTACTG GGGTTTCCAC AGTCACTTCA 
     2581 GCAGAGAAGC AGAGCACTTG TACCACACCT TGGAGTCCTC CTACCAGAAA GCCCTGCAGT 
     2641 CCCACCTGAA GAACTCAGAC AGCATAGTGT CTCTGCCTCA GTCAGACCGC TCATCTTCCA 
     2701 GCTCTCAAGA GAGTCTAAAT CGTCCGCTGT CTGCCAAAAG AAGTCCTACT GGAAGTACCA 
     2761 CATCTAGAGC TTCTACAGTT AGTACCAAAT CTGTGTCAAC GACTGGGTCC CTCCAGCGAT 
     2821 CTCGAAGTGA TATTGATGTG AACGCAGCAG CCAGTGCCAA ATCCAAAGTC TCCTCATCTT 
     2881 CGGGCACGAC GCCTTTCAGC TCTGCAGCAG CTTTGCCTCC AGGGTCATAC GCATCCTTAG 
     2941 ATGGTACTAC CACTAAAGCG GAGGGTCGGA TCCGCACAAG ACGGCAAAGC TCTGGGAGTG 
     3001 CCACCAACGT CGCCTCTACA CCTGATAACC GGGGCCGCAG TCGCGCTAAA GTGGTTTCAC 
     3061 AGTCCCAGCG ATCCAGATCT GCTAATCCTG CTGGTGCTGG CAGCCGGTCA AGTTCCCCAG 
     3121 GAAAATTGTT GGGAAGTGGT TATGGTGGAC TTACTGGGGG CTCCTCACGA GGCCCACCTG 
     3181 TGACACCGTC TTCAGAAAAG CGAAGCAAGA TTCCCAGGAG CCAGGGATGT AGCCGGGAAA 
     3241 CAAGTCCAAA CCGAATAGGA TTAGATCGGT TTGGGCTTGG CCAGCCAGGA AGAATACCTG 
     3301 GTTCTGTGAA TGCCATGAGA GTTCTGAGCA CAAGTACAGA TCTTGAAGCT GCTGTTGCTG 
     3361 ATGCTTTGCT GTTAGGAGAC TCCAGGAGCA AGAAGAAGCC TGTGAGGAGG AGATATGAGC 
     3421 CGTATGGGAT GTATTCTGAC GATGATGCCA ACAGTGATGC CTCAAGTGTT TGCTCTGAGC 
     3481 GCTCATATGG CTCCAGGAAT GGTGGCATTC CCCATTATCT GCGGCAGACT GAGGATGTAG 
     3541 CAGAAGTTCT CAACCACTGT GCTAGTTCAA ACTGGTCAGA AAGGAAAGAA GGGCTTCTGG 
     3601 GCCTGCAGAA CTTACTGAAG AGCCAAAGAA CACTGAGTCG AGTTGAACTG AAAAGGTTGT 
     3661 GTGAGATCTT CACTCGGATG TTTGCTGACC CTCATAGCAA GAGAGTTTTC AGTATGTTTT 
     3721 TGGAGACTCT TGTGGATTTT ATAATAATTC ATAAGGATGA TTTACAAGAC TGGCTTTTTG 
     3781 TTCTTCTCAC ACAATTACTT AAGAAAATGG GAGCAGATTT ACTTGGATCT GTGCAAGCAA 
     3841 AAGTTCAAAA GGCTCTAGAT GTCACAAGGG ACTCCTTTCC ATTTGATCAA CAATTTAACA 
     3901 TTTTGATGAG ATTTATTGTG GATCAAACTC AAACTCCAAA CCTCAAGGTC AAAGTTGCAA 
     3961 TCCTGAAATA CATTGAGTCT CTGGCCAGAC AGATGGATCC AACAGATTTT GTAAACTCTA 
     4021 GTGAGACAAG GCTTGCTGTT TCTAGAATCA TAACCTGGAC AACAGAACCA AAGAGTTCAG 
     4081 ACGTGAGAAA GGCAGCACAG ATTGTGCTAA TCTCTCTGTT TGAATTGAAT ACTCCTGAAT 
     4141 TTACCATGTT ACTTGGTGCC TTGCCAAAAA CATTCCAGGA TGGTGCCACC AAACTCCTGC 
     4201 ACAACCACCT CAAGAATTCC AGTAACACCA GTGTGGGCTC TCCAAGCAAT ACGATTGGCC 
     4261 GGACGCCCTC CCGACACACC AGCAGCAGGA CCAGCCCCCT GACCTCACCC ACCAACTGTT 
     4321 CCCATGGGGG TCTGTCTCCA AGCATGCTGG ACTATGATAC AGAGAACCTG AACTCTGAAG 
     4381 AAATCTATAG TTCTCTACGT GGAGTTACAG AAGCCATTGA AAAGTTTAGT TTTCGAAGCC 
     4441 AAGAAGATCT GAATGAGCCA ATTAAACGAG ATGGCAAAAA GGAGTGTGAT ATTGTGTCCC 
     4501 GCGATGGGGG CGCTGCCTCC CCTGCCACTG AGGGCCGGGG GGGTAGTGAA GTAGAAGGAG 
     4561 GCCGGACAGC TCTGGATAAC AAGACCTCAC TACTCAACAC CCAGCCTCCG CGCGCCTTCC 
     4621 CGGGGCCGCG GGCGCGAGAC TACAACCCGT ACCCCTACTC AGATGCCATC AACACCTACG 
     4681 ACAAGACCGC CCTGAAAGAG GCTGTGTTCG ATGACGACAT GGAGCAGCTT CGAGACGTGC 
     4741 CCATCGACCA TTCTGACCTG GTGGCTGACC TTCTGAAAGA GCTGTCCAAC CACAATGAGC 
     4801 GAGTGGAGGA ACGGAAGGGA GCCCTGCTGG AGCTGCTCAA GATCACGCGG GAAGACAGCC 
     4861 TTGGTGTCTG GGAGGAGCAC TTCAAGACCA TTCTGCTCCT GCTGCTGGAG ACCCTTGGAG 
     4921 ACAAAGACCA TTCAATTCGA GCACTGGCGT TAAGAGTTTT GAGGGAAATT CTGAGAAATC 
     4981 AACCAGCAAG ATTTAAAAAC TACGCCGAGC TGACGATTAT GAAGACTCTG GAAGCCCACA 
     5041 AAGACTCCCA TAAGGAGGTG GTGAGAGCGG CTGAGGAGGC TGCGTCCACA CTGGCCAGTT 
     5101 CCATCCACCC GGAGCAGTGC ATCAAGGTGC TCTGCCCCAT CATCCAGACG GCCGACTACC 
     5161 CCATCAACCT TGCTGCCATC AAGATGCAGA CCAAAGTCGT CGAGAGGATC GCAAAGGAGT 
     5221 CATTGCTGCA GCTCCTTGTC GACATCATCC CAGGCTTGCT GCAGGGTTAT GACAACACCG 
     5281 AAAGTAGTGT GCGTAAGGCC AGCGTGTTTT GCTTAGTGGC AATTTATTCC GTAATCGGAG 
     5341 AAGACCTGAA ACCTCACCTT GCACAGCTCA CAGGGAGCAA GATGAAGCTA CTAAACTTAT 
     5401 ACATAAAGAG GGCCCAGACC ACCAACAGCA ACAGCAGCTC CTCCTCCGAT GTCTCCACGC 
     5461 ACAGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5521 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5581 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5641 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5701 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5761 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5821 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5881 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5941 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6001 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6061 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6121 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6181 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6241 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6301 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6361 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6421 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6481 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6541 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6601 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6661 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6721 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6781 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6841 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6901 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6961 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7021 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7081 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7141 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7201 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7261 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7321 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7381 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7441 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7501 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7561 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7621 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7681 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7741 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7801 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7861 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7921 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7981 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8041 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8101 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8161 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8221 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8281 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8341 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8401 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8461 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8521 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8581 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8641 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8761 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8821 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8881 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8941 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9001 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9061 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9121 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9181 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9241 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9301 AAATTTAACG CGAATTTT
//