LOCUS       RC226572                8778 bp    DNA     circular  SYN 
DEFINITION  NFRKB (Myc-DDK-tagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 1
ACCESSION   NM_001143835
VERSION     NM_001143835.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4935..5026 
                     /label="Myc-DDK"  
     misc_feature    5056..5641 
                     /label="PolyA signal"  
     misc_feature    5113..5132 
                     /label="Restriction Enzyme"  
     misc_feature    5917..6589 
                     /label="ColE1"  
     misc_feature    7019..7813 
                     /label="Kan/Neo"  
     misc_feature    7848..8150 
                     /label="SV40 ori"  
     misc_feature    8236..8713 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4917..4923 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4917 
                     /label="NFRKB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCCTTA GACCATATGC TGACAGATCC TCTGGAACTT GGTCCGTGTG 
     1081 GAGATGGCCA TGGCACGCGC ATCATGGAGG ATTGCCTCCT GGGAGGCACC AGAGTTAGTC 
     1141 TGCCCGAGGA CCTTCTGGAG GATCCTGAGA TCTTCTTTGA TGTTGTCAGC CTCTCAACAT 
     1201 GGCAGGAAGT GTTAAGTGAT TCTCAACGTG AACACCTCCA GCAGTTTCTG CCCCAGTTTC 
     1261 CTGAAGACAG TGCTGAGCAG CAGAATGAAC TCATCTTAGC CTTGTTCAGT GGGGAGAACT 
     1321 TCCGCTTTGG AAACCCTCTG CACATTGCCC AGAAGCTTTT CCGAGACGGA CACTTTAACC 
     1381 CCGAGGTGGT CAAGTACCGG CAGTTATGCT TCAAGTCACA GTACAAGCGC TACCTCAACT 
     1441 CCCAGCAGCA GTATTTCCAT CGGCTGCTGA AGCAAATTCT TGCTTCCCGG AGTGATCTGC 
     1501 TGGAGATGGC CCGGCGGAGT GGCCCCGCCC TTCCCTTCCG GCAGAAACGC CCTTCACCAT 
     1561 CCCGCACACC TGAGGAGCGG GAGTGGCGGA CCCAGCAGCG CTACTTGAAG GTCTTAAGGG 
     1621 AAGTGAAAGA GGAGTGTGGT GACACAGCCC TGTCATCTGA TGAAGAGGAT CTCAGCTCAT 
     1681 GGCTTCCGAG CTCTCCAGCA CGTTCTCCTA GTCCTGCGGT GCCCCTGCGG GTGGTGCCCA 
     1741 CACTTTCAAC CACGGATATG AAAACTGCAG ATAAAGTAGA ACTGGGGGAC AGTGACCTGA 
     1801 AGATAATGTT AAAGAAGCAC CACGAGAAGC GGAAACATCA GCCAGATCAC CCGGACCTTT 
     1861 TGACAGGGGA CCTGACTCTC AATGACATCA TGACTCGAGT AAATGCTGGC AGGAAGGGCT 
     1921 CTCTGGCAGC CTTATATGAC TTGGCTGTCC TTAAAAAAAA GGTTAAGGAA AAAGAGGAAA 
     1981 AGAAGAAGAA GAAAATAAAA ACGATCAAAT CAGAGGCAGA GGACCTGGCC GAGCCGCTAA 
     2041 GCAGTACTGA AGGGGTCGCA CCTCTCTCAC AGGCCCCCTC TCCGCTGGCA ATTCCTGCTA 
     2101 TCAAGGAAGA GCCCCTTGAA GACCTCAAGC CTTGCCTTGG AATCAATGAA ATATCTTCCA 
     2161 GCTTCTTCTC TCTTCTATTA GAGATCTTGC TGCTGGAGAG TCAGGCTAGC CTTCCTATGC 
     2221 TAGAGGAGCG AGTTTTGGAT TGGCAGTCAT CGCCAGCCAG CTCCCTCAAC AGCTGGTTCT 
     2281 CTGCGGCCCC CAACTGGGCT GAGTTGGTAC TACCAGCCCT GCAGTATCTT GCTGGAGAAA 
     2341 GTCGAGCTGT TCCTTCCAGT TTCTCTCCAT TTGTTGAATT CAAAGAGAAA ACCCAGCAGT 
     2401 GGAAGTTGCT TGGCCAATCC CAAGATAATG AAAAGGAATT AGCTGCCCTC TTCCAGCTAT 
     2461 GGCTAGAGAC CAAAGATCAG GCCTTCTGTA AGCAAGAAAA TGAAGACAGC TCAGATGCCA 
     2521 CAACACCTGT CCCTCGGGTA AGAACTGACT ATGTGGTGCG TCCCAGCACG GGGGAGGAGA 
     2581 AACGGGTTTT TCAGGAGCAG GAGCGTTACA GGTATAGCCA ACCCCATAAG GCGTTCACCT 
     2641 TTCGCATGCA CGGCTTTGAG TCTGTGGTGG GGCCAGTGAA GGGCGTGTTT GACAAGGAGA 
     2701 CCTCGCTCAA CAAGGCTCGG GAGCACTCCC TGCTGCGCTC CGACCGGCCT GCCTACGTCA 
     2761 CCATTCTGTC TCTTGTTCGG GACGCTGCGG CTCGACTGCC TAATGGAGAA GGCACACGGG 
     2821 CAGAGATCTG TGAACTGCTT AAGGACTCCC AGTTTCTTGC ACCAGATGTC ACCAGCACTC 
     2881 AGGTAAATAC AGTAGTGAGT GGTGCACTGG ATCGGCTACA TTACGAAAAA GATCCCTGTG 
     2941 TGAAATACGA CATTGGACGA AAGCTGTGGA TCTACCTGCA TCGTGACCGG AGTGAAGAAG 
     3001 AGTTTGAGCG GATTCACCAA GCACAAGCAG CTGCAGCTAA AGCCAGAAAA GCTCTTCAGC 
     3061 AAAAACCCAA GCCCCCATCC AAGGTGAAGT CCAGTAGCAA GGAGAGCTCC ATAAAGGTCC 
     3121 TTAGCAGTGG CCCTTCTGAG CAGAGCCAGA TGAGCCTCAG TGACTCCAGT ATGCCACCCA 
     3181 CCCCAGTCAC ACCTGTAACC CCCACCACAC CAGCATTGCC CGCCATTCCC ATCTCCCCTC 
     3241 CACCTGTATC GGCAGTGAAC AAAAGCGGCC CTTCCACAGT CTCAGAACCA GCTAAGTCTA 
     3301 GCTCGGGTGT TCTTCTGGTG TCTTCACCAA CAATGCCACA TCTGGGAACA ATGCTTTCCC 
     3361 CAGCTTCCAG CCAGACTGCA CCCAGTTCTC AGGCTGCCGC CCGGGTCGTG AGCCACTCTG 
     3421 GCTCTGCTGG ACTGTCTCAG GTGCGAGTGG TGGCCCAGCC TAGCCTTCCT GCTGTTCCCC 
     3481 AGCAGTCGGG AGGGCCGGCA CAGACATTGC CACAGATGCC AGCAGGACCG CAGATCCGGG 
     3541 TTCCAGCCAC TGCCACACAG ACCAAAGTAG TGCCCCAGAC AGTAATGGCC ACTGTGCCCG 
     3601 TCAAAGCGCA GACTACGGCA GCCACTGTGC AGCGGCCTGG ACCCGGGCAG ACAGGGCTCA 
     3661 CGGTGACAAG TCTCCCTGCC ACAGCCAGCC CTGTGAGTAA GCCAGCCACG AGTTCTCCTG 
     3721 GGACCTCTGC TCCCAGTGCC TCCACGGCTG CCGTCATTCA AAATGTCACA GGACAGAACA 
     3781 TCATCAAGCA GGTGGCAATC ACTGGGCAGC TTGGTGTGAA GCCCCAAACA GGCAACAGCA 
     3841 TTCCACTCAC AGCCACTAAC TTCCGCATCC AGGGTAAGGA TGTATTGCGT CTGCCGCCCT 
     3901 CTTCCATCAC CACAGATGCC AAGGGCCAGA CGGTTCTGCG AATCACTCCG GACATGATGG 
     3961 CCACATTGGC CAAGTCCCAG GTTACCACAG TCAAATTGAC CCAGGACCTC TTCGGGACAG 
     4021 GAGGCAACAC TACAGGCAAA GGCATCTCTG CCACCTTACA CGTCACTTCC AATCCAGTAC 
     4081 ATGCAGCTGA TAGCCCTGCC AAGGCCAGTT CAGCCAGTGC CCCTTCATCC ACTCCAACAG 
     4141 GTACCACTGT GGTCAAAGTG ACTCCTGACC TCAAGCCAAC AGAAGCCTCA AGTTCGGCTT 
     4201 TTCGCTTGAT GCCAGCTCTT GGCGTGAGTG TGGCTGACCA GAAGGGAAAA AGCACAGTGG 
     4261 CCTCTTCAGA AGCAAAACCA GCTGCCACGA TCCGCATCGT GCAGGGACTG GGAGTGATGC 
     4321 CTCCCAAAGC AGGCCAGACC ATCACCGTTG CAACCCACGC CAAGCAAGGG GCCTCGGTGG 
     4381 CCAGTGGGTC TGGAACTGTC CATACTTCAG CGGTGTCCTT ACCCAGTATG AATGCTGCTG 
     4441 TGTCCAAGAC TGTAGCTGTG GCTTCTGGGG CTGCAAGCAC CCCCATCAGC ATCAGCACAG 
     4501 GAGCCCCCAC CGTGCGGCAG GTCCCTGTCA GCACCACGGT TGTGTCCACG TCCCAGGCTG 
     4561 AGAAGTTGCC TACACGGATC ACAGTTCCCC TCTCTGTGAT CAGCCAGCCA ATGAAGGGCA 
     4621 AGAGCGTGGT CACAGCCCCC ATCATCAAAG GCAACCTTGG AGCCGACCTC AGTGGGTTGG 
     4681 GCCGCAACAT CATCCTCACA ACTATGCCAG CAGGCACTAA GCTCATTGCT GGCAATAAGC 
     4741 CTGTTAGTTT CCTCACTGCT CAGCAGTTGC AGCAGCTTCA GCAGCAAGGT CAGGCCACAC 
     4801 AGGTGCGCAT CCAGACTGTC CCTGCATCCC ATCTCCAACA GGGAACAGCT TCTGGCTCCT 
     4861 CCAAAGCAGT CTCCACTGTT GTTGTGACTA CAGCTCCGTC TCCTAAACAG GCACCTGAGC 
     4921 AACAAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4981 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5041 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5101 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5161 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5221 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5281 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5341 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5401 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5461 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5521 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5581 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5641 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5701 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5761 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5821 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5881 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5941 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6001 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6061 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6121 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6181 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6241 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6301 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6361 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6421 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6481 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6541 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6601 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6661 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6721 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6781 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6841 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6901 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6961 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7021 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7081 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7141 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7201 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7261 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7321 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7381 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7441 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7501 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7561 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7621 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7681 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7741 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7801 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7861 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7921 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7981 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8221 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8281 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8341 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8401 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8461 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8521 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8581 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8641 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8701 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8761 AAATTTAACG CGAATTTT
//