LOCUS       RC226402                9408 bp    DNA     circular  SYN 
DEFINITION  CPS1 (Myc-DDK-tagged)-Human carbamoyl-phosphate synthase 1, mitochondrial (CPS1), nuclear gene encoding mitochondrial protein, transcript variant 1
ACCESSION   NM_001122633
VERSION     NM_001122633.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5563..5654 
                     /label="Myc-DDK"  
     misc_feature    5684..6269 
                     /label="PolyA signal"  
     misc_feature    5741..5760 
                     /label="Restriction Enzyme"  
     misc_feature    6545..7217 
                     /label="ColE1"  
     misc_feature    7647..8441 
                     /label="Kan/Neo"  
     misc_feature    8476..8778 
                     /label="SV40 ori"  
     misc_feature    8864..9341 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5538..5545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5538 
                     /label="CPS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACAAATC ATCAAAATGA CGAGGATTTT GACAGCTTTC AAAGTGGTGA 
     1081 GGACACTGAA GACTGGTTTT GGCTTTACCA ATGTGACTGC ACACCAAAAA TGGAAATTTT 
     1141 CAAGACCTGG CATCAGGCTC CTTTCTGTCA AGGCACAGAC AGCACACATT GTCCTGGAAG 
     1201 ATGGAACTAA GATGAAAGGT TACTCCTTTG GCCATCCATC CTCTGTTGCT GGTGAAGTGG 
     1261 TTTTTAATAC TGGCCTGGGA GGGTACCCAG AAGCTATTAC TGACCCTGCC TACAAAGGAC 
     1321 AGATTCTCAC AATGGCCAAC CCTATTATTG GGAATGGTGG AGCTCCTGAT ACTACTGCTC 
     1381 TGGATGAACT GGGACTTAGC AAATATTTGG AGTCTAATGG AATCAAGGTT TCAGGTTTGC 
     1441 TGGTGCTGGA TTATAGTAAA GACTACAACC ACTGGCTGGC TACCAAGAGT TTAGGGCAAT 
     1501 GGCTACAGGA AGAAAAGGTT CCTGCAATTT ATGGAGTGGA CACAAGAATG CTGACTAAAA 
     1561 TAATTCGGGA TAAGGGTACC ATGCTTGGGA AGATTGAATT TGAAGGTCAG CCTGTGGATT 
     1621 TTGTGGATCC AAATAAACAG AATTTGATTG CTGAGGTTTC AACCAAGGAT GTCAAAGTGT 
     1681 ACGGCAAAGG AAACCCCACA AAAGTGGTAG CTGTAGACTG TGGGATTAAA AACAATGTAA 
     1741 TCCGCCTGCT AGTAAAGCGA GGAGCTGAAG TGCACTTAGT TCCCTGGAAC CATGATTTCA 
     1801 CCAAGATGGA GTATGATGGG ATTTTGATCG CGGGAGGACC GGGGAACCCA GCTCTTGCAG 
     1861 AACCACTAAT TCAGAATGTC AGAAAGATTT TGGAGAGTGA TCGCAAGGAG CCATTGTTTG 
     1921 GAATCAGTAC AGGAAACTTA ATAACAGGAT TGGCTGCTGG TGCCAAAACC TACAAGATGT 
     1981 CCATGGCCAA CAGAGGGCAG AATCAGCCTG TTTTGAATAT CACAAACAAA CAGGCTTTCA 
     2041 TTACTGCTCA GAATCATGGC TATGCCTTGG ACAACACCCT CCCTGCTGGC TGGAAACCAC 
     2101 TTTTTGTGAA TGTCAACGAT CAAACAAATG AGGGGATTAT GCATGAGAGC AAACCCTTCT 
     2161 TCGCTGTGCA GTTCCACCCA GAGGTCACCC CGGGGCCAAT AGACACTGAG TACCTGTTTG 
     2221 ATTCCTTTTT CTCACTGATA AAGAAAGGAA AAGCTACCAC CATTACATCA GTCTTACCGA 
     2281 AGCCAGCACT AGTTGCATCT CGGGTTGAGG TTTCCAAAGT CCTTATTCTA GGATCAGGAG 
     2341 GTCTGTCCAT TGGTCAGGCT GGAGAATTTG ATTACTCAGG ATCTCAAGCT GTAAAAGCCA 
     2401 TGAAGGAAGA AAATGTCAAA ACTGTTCTGA TGAACCCAAA CATTGCATCA GTCCAGACCA 
     2461 ATGAGGTGGG CTTAAAGCAA GCGGATACTG TCTACTTTCT TCCCATCACC CCTCAGTTTG 
     2521 TCACAGAGGT CATCAAGGCA GAACAGCCAG ATGGGTTAAT TCTGGGCATG GGTGGCCAGA 
     2581 CAGCTCTGAA CTGTGGAGTG GAACTATTCA AGAGAGGTGT GCTCAAGGAA TATGGTGTGA 
     2641 AAGTCCTGGG AACTTCAGTT GAGTCCATTA TGGCTACGGA AGACAGGCAG CTGTTTTCAG 
     2701 ATAAACTAAA TGAGATCAAT GAAAAGATTG CTCCAAGTTT TGCAGTGGAA TCGATTGAGG 
     2761 ATGCACTGAA GGCAGCAGAC ACCATTGGCT ACCCAGTGAT GATCCGTTCC GCCTATGCAC 
     2821 TGGGTGGGTT AGGCTCAGGC ATCTGTCCCA ACAGAGAGAC TTTGATGGAC CTCAGCACAA 
     2881 AGGCCTTTGC TATGACCAAC CAAATTCTGG TGGAGAAGTC AGTGACAGGT TGGAAAGAAA 
     2941 TAGAATATGA AGTGGTTCGA GATGCTGATG ACAATTGTGT CACTGTCTGT AACATGGAAA 
     3001 ATGTTGATGC CATGGGTGTT CACACAGGTG ACTCAGTTGT TGTGGCTCCT GCCCAGACAC 
     3061 TCTCCAATGC CGAGTTTCAG ATGTTGAGAC GTACTTCAAT CAATGTTGTT CGCCACTTGG 
     3121 GCATTGTGGG TGAATGCAAC ATTCAGTTTG CCCTTCATCC TACCTCAATG GAATACTGCA 
     3181 TCATTGAAGT GAATGCCAGA CTGTCCCGAA GCTCTGCTCT GGCCTCAAAA GCCACTGGCT 
     3241 ACCCATTGGC ATTCATTGCT GCAAAGATTG CCCTAGGAAT CCCACTTCCA GAAATTAAGA 
     3301 ACGTCGTATC CGGGAAGACA TCAGCCTGTT TTGAACCTAG CCTGGATTAC ATGGTCACCA 
     3361 AGATTCCCCG CTGGGATCTT GACCGTTTTC ATGGAACATC TAGCCGAATT GGTAGCTCTA 
     3421 TGAAAAGTGT AGGAGAGGTC ATGGCTATTG GTCGTACCTT TGAGGAGAGT TTCCAGAAAG 
     3481 CTTTACGGAT GTGCCACCCA TCTATAGAAG GTTTCACTCC CCGTCTCCCA ATGAACAAAG 
     3541 AATGGCCATC TAATTTAGAT CTTAGAAAAG AGTTGTCTGA ACCAAGCAGC ACGCGTATCT 
     3601 ATGCCATTGC CAAGGCCATT GATGACAACA TGTCCCTTGA TGAGATTGAG AAGCTCACAT 
     3661 ACATTGACAA GTGGTTTTTG TATAAGATGC GTGATATTTT AAACATGGAA AAGACACTGA 
     3721 AAGGCCTCAA CAGTGAGTCC ATGACAGAAG AAACCCTGAA AAGGGCAAAG GAGATTGGGT 
     3781 TCTCAGATAA GCAGATTTCA AAATGCCTTG GGCTCACTGA GGCCCAGACA AGGGAGCTGA 
     3841 GGTTAAAGAA AAACATCCAC CCTTGGGTTA AACAGATTGA TACACTGGCT GCAGAATACC 
     3901 CATCAGTAAC AAACTATCTC TATGTTACCT ACAATGGTCA GGAGCATGAT GTCAATTTTG 
     3961 ATGACCATGG AATGATGGTG CTAGGCTGTG GTCCATATCA CATTGGCAGC AGTGTGGAAT 
     4021 TTGATTGGTG TGCTGTCTCT AGTATCCGCA CACTGCGTCA ACTTGGCAAG AAGACGGTGG 
     4081 TGGTGAATTG CAATCCTGAG ACTGTGAGCA CAGACTTTGA TGAGTGTGAC AAACTGTACT 
     4141 TTGAAGAGTT GTCCTTGGAG AGAATCCTAG ACATCTACCA TCAGGAGGCA TGTGGTGGCT 
     4201 GCATCATATC AGTTGGAGGC CAGATTCCAA ACAACCTGGC AGTTCCTCTA TACAAGAATG 
     4261 GTGTCAAGAT CATGGGCACA AGCCCCCTGC AGATCGACAG GGCTGAGGAT CGCTCCATCT 
     4321 TCTCAGCTGT CTTGGATGAG CTGAAGGTGG CTCAGGCACC TTGGAAAGCT GTTAATACTT 
     4381 TGAATGAAGC ACTGGAATTT GCAAAGTCTG TGGACTACCC CTGCTTGTTG AGGCCTTCCT 
     4441 ATGTTTTGAG TGGGTCTGCT ATGAATGTGG TATTCTCTGA GGATGAGATG AAAAAATTCC 
     4501 TAGAAGAGGC GACTAGAGTT TCTCAGGAGC ACCCAGTGGT GCTGACAAAA TTTGTTGAAG 
     4561 GGGCCCGAGA AGTAGAAATG GACGCTGTTG GCAAAGATGG AAGGGTTATC TCTCATGCCA 
     4621 TCTCTGAACA TGTTGAAGAT GCAGGTGTCC ACTCGGGAGA TGCCACTCTG ATGCTGCCCA 
     4681 CACAAACCAT CAGCCAAGGG GCCATTGAAA AGGTGAAGGA TGCTACCCGG AAGATTGCAA 
     4741 AGGCTTTTGC CATCTCTGGT CCATTCAACG TCCAATTTCT TGTCAAAGGA AATGATGTCT 
     4801 TGGTGATTGA GTGTAACTTG AGAGCTTCTC GATCCTTCCC CTTTGTTTCC AAGACTCTTG 
     4861 GGGTTGACTT CATTGATGTG GCCACCAAGG TGATGATTGG AGAGAATGTT GATGAGAAAC 
     4921 ATCTTCCAAC ATTGGACCAT CCCATAATTC CTGCTGACTA TGTTGCAATT AAGGCTCCCA 
     4981 TGTTTTCCTG GCCCCGGTTG AGGGATGCTG ACCCCATTCT GAGATGTGAG ATGGCTTCCA 
     5041 CTGGAGAGGT GGCTTGCTTT GGTGAAGGTA TTCATACAGC CTTCCTAAAG GCAATGCTTT 
     5101 CCACAGGATT TAAGATACCC CAGAAAGGCA TCCTGATAGG CATCCAGCAA TCATTCCGGC 
     5161 CAAGATTCCT TGGTGTGGCT GAACAATTAC ACAATGAAGG TTTCAAGCTG TTTGCCACGG 
     5221 AAGCCACATC AGACTGGCTC AACGCCAACA ATGTCCCTGC CACCCCAGTG GCATGGCCGT 
     5281 CTCAAGAAGG ACAGAATCCC AGCCTCTCTT CCATCAGAAA ATTGATTAGA GATGGCAGCA 
     5341 TTGACCTAGT GATTAACCTT CCCAACAACA ACACTAAATT TGTCCATGAT AATTATGTGA 
     5401 TTCGGAGGAC AGCTGTTGAT AGTGGAATCC CTCTCCTCAC TAATTTTCAG GTGACCAAAC 
     5461 TTTTTGCTGA AGCTGTGCAG AAATCTCGCA AGGTGGACTC CAAGAGTCTT TTCCACTACA 
     5521 GGCAGTACAG TGCTGGAAAA GCAGCAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5581 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5641 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5701 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5761 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5821 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5881 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5941 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     6001 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     6061 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     6121 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6181 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6241 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6301 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6361 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6421 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6481 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6541 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6601 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6661 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6721 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6781 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6841 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6901 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6961 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7021 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7081 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     7141 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7201 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7261 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7321 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7381 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7441 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7501 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7561 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7621 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7681 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7741 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7801 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7861 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7921 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7981 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     8041 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     8101 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     8161 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8221 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8281 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8341 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8401 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8461 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8521 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8581 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8641 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8701 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8761 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8821 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8881 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8941 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     9001 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     9061 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     9121 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9181 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9241 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9301 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9361 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//