LOCUS       RC226400                9345 bp    DNA     circular  SYN 
DEFINITION  IQSEC2 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 1
ACCESSION   NM_001111125
VERSION     NM_001111125.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5502..5593 
                     /label="Myc-DDK"  
     misc_feature    5623..6208 
                     /label="PolyA signal"  
     misc_feature    5680..5699 
                     /label="Restriction Enzyme"  
     misc_feature    6484..7156 
                     /label="ColE1"  
     misc_feature    7586..8380 
                     /label="Kan/Neo"  
     misc_feature    8415..8717 
                     /label="SV40 ori"  
     misc_feature    8803..9280 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5484..5490 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5484 
                     /label="IQSEC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGGGG TCGGGGCCCC CGGGCGGCCC GGGATCCGAG AGCCCAAATC 
     1081 GGGCCGTGGA GTACCTGCTG GAGCTGAACA ACATCATCGA GAGCCAGCAG CAGCTGCTGG 
     1141 AAACCCAGCG GCGGCGCATC GAGGAGCTGG AGGGCCAGCT GGACCAGCTC ACCCAGGAGA 
     1201 ACCGCGACCT GCGAGAGGAG AGCCAGCTGC ACCGCGGGGA GCTGCACCGG GACCCCCACG 
     1261 GCGCGCGGGA TAGCCCGGGC CGCGAGAGCC AGTACCAGAA CCTGCGCGAG ACCCAGTTCC 
     1321 ACCACCGCGA GCTGCGGGAG AGCCAGTTCC ACCAGGCGGC CCGGGACGTG GGCTACCCGA 
     1381 ACCGGGAAGG CGCCTACCAG AATCGGGAGG CTGTGTATCG GGACAAGGAG CGGGACGCCT 
     1441 CCTACCCGCT CCAGGACACT ACCGGTTACA CAGCCCGCGA GCGTGACGTG GCCCAGTGCC 
     1501 ACCTGCACCA CGAGAACCCA GCCCTGGGTC GCGAGCGTGG CGGGCGGGAG GCCGGGCCGG 
     1561 CGCACCCGGG CCGCGAGAAG GAAGCGGGCT ATTCGGCGGC GGTGGGCGTG GGGCCGCGGC 
     1621 CACCGCGGGA GCGGGGCCAG CTGAGCCGTG GCGCATCCAG GAGCTCCAGT CCCGGCGCCG 
     1681 GCGGAGGCCA CAGCACCAGT ACCAGCACCA GCCCGGCCAC GACCCTCCAG AGAAAATCTG 
     1741 ATGGTGAGAA TTCCAGAACA GTCAGTGTGG AGGGTGATGC CCCAGGCAGT GACCTGAGCA 
     1801 CAGCGGTTGA TAGTCCTGGG AGCCAACCCC CCTACCGGCT GAGCCAGCTG CCCCCCTCCA 
     1861 GCAGCCACAT GGGGGGCCCC CCTGCTGGAG TGGGCCTTCC CTGGGCTCAG CGGGCACGCC 
     1921 TCCAGCCAGC CAGTGTCGCC CTGAGGAAGC AGGAGGAGGA GGAGATAAAG CGCTCCAAGG 
     1981 CCCTATCGGA CAGCTATGAA CTCTCCACAG ACCTGCAGGA CAAGAAGGTG GAAATGCTGG 
     2041 AGAGGAAGTA TGGGGGCTCC TTCCTGAGCC GCAGGGCTGC CAGGACCATC CAGACAGCCT 
     2101 TCCGCCAGTA CCGCATGAAC AAGAACTTTG AGCGGCTACG CAGCTCAGCC TCAGAGAGCC 
     2161 GCATGTCCCG CCGCATCATC CTTTCCAACA TGCGGATGCA GTTCTCCTTT GAGGAGTATG 
     2221 AGAAGGCACA GAACCCCGCG TACTTCGAGG GCAAGCCTGC CTCGCTGGAC GAGGGTGCCA 
     2281 TGGCTGGTGC CCGGAGCCAC CGGCTTGAAC GGGGGCTCCC ATATGGAGGC TCCTGTGGTG 
     2341 GGGGCATCGA TGGTGGTGGA AGCTCCGTCA CCACATCTGG AGAGTTTTCT AATGACATCA 
     2401 CAGAACTTGA GGACTCCTTC TCCAAACAGG TAAAGTCTCT GGCTGAATCC ATCGACGAAG 
     2461 CCCTGAACTG CCACCCGTCA GGGCCCATGT CTGAGGAGCC AGGGTCAGCC CAGCTGGAGA 
     2521 AGCGGGAGTC AAAGGAACAG CAAGAGGACA GCTCAGCCAC ATCCTTCAGT GATCTTCCCC 
     2581 TCTACCTGGA TGACACAGTT CCCCAACAAT CCCCTGAGCG ACTGCCCAGC ACAGAACCCC 
     2641 CACCCCAGGG CCGGCCCGAG TTCTGGGCGC CAGCCCCTCT CCCGCCAGTT CCTCCACCAG 
     2701 TGCCATCAGG AACCCGGGAA GACGGTAGCC GTGAGGAAGG CACTCGCAGG GGTCCCGGGT 
     2761 GCTTGGAGTG CCGGGATTTC CGGCTGCGGG CTGCCCACCT TCCCCTGCTT ACCATTGAGC 
     2821 CTCCTAGTGA CAGCTCCGTG GACCTGAGTG ACCGCTCAGA TCGCGGCTCT GTCCACCGCC 
     2881 AGCTGGTGTA TGAGGCTGAT GGCTGCAGCC CCCATGGGAC CCTGAAGCAC AAGGGGCCAC 
     2941 CAGGCAGGGC CCCGATCCCA CACCGCCACT ACCCAGCCCC TGAAGGCCCA GCCCCAGCCC 
     3001 CACCAGGGCC CCTGCCACCA GCCCCCAACA GTGGCACTGG GCCCAGTGGT GTGGCTGGGG 
     3061 GTCGGAGGTT GGGGAAGTGC GAGGCAGCAG GCGAGAACTC TGATGGTGGA GATAACGAGA 
     3121 GCCTTGAGAG CTCCAGCAAT TCCAATGAGA CCATCAACTG CAGCTCCGGC TCCTCTTCTC 
     3181 GGGACAGTCT AAGGGAGCCT CCGGCTACCG GCCTGTGCAA GCAGACTTAC CAGCGGGAGA 
     3241 CAAGGCATAG CTGGGACTCG CCAGCTTTCA ACAATGATGT GGTCCAGAGG CGGCACTACC 
     3301 GAATCGGCCT CAACCTCTTC AACAAGAAGC CAGAGAAGGG TATCCAGTAT CTGATCGAGC 
     3361 GGGGCTTCCT GTCAGACACA CCGGTGGGAG TGGCTCACTT CATCCTGGAG CGGAAAGGCC 
     3421 TCAGCCGGCA GATGATAGGG GAATTCCTAG GGAACCGGCA GAAGCAGTTC AACAGAGACG 
     3481 TGTTGGACTG TGTGGTGGAT GAGATGGACT TCTCCTCCAT GGATCTGGAT GATGCGCTCC 
     3541 GGAAGTTCCA GTCCCATATC CGGGTTCAGG GTGAGGCCCA GAAAGTGGAG CGACTCATCG 
     3601 AAGCCTTCAG CCAGCGGTAC TGTGTCTGTA ACCCAGCCCT CGTGCGCCAG TTCCGGAACC 
     3661 CAGACACCAT CTTCATCCTT GCTTTTGCCA TCATCCTCCT CAATACCGAC ATGTACAGTC 
     3721 CCAGCGTCAA AGCTGAACGA AAGATGAAAC TAGATGACTT CATCAAGAAC CTGAGAGGGG 
     3781 TTGACAATGG TGAAGACATC CCCCGAGACC TCCTGGTGGG CATCTACCAG CGCATCCAGG 
     3841 GGCGTGAACT GCGGACCAAC GATGACCATG TGTCCCAGGT GCAGGCTGTG GAGCGCATGA 
     3901 TTGTTGGAAA GAAACCAGTC CTGTCTCTCC CTCACCGTCG ACTGGTTTGC TGCTGCCAGC 
     3961 TCTACGAGGT GCCAGATCCA AACCGCCCCC AGAGGCTAGG GTTGCATCAG CGGGAGGTCT 
     4021 TCCTCTTCAA TGATCTCCTT GTGGTCACCA AAATTTTCCA GAAGAAGAAG ATCTTGGTGA 
     4081 CGTACAGTTT CCGTCAGTCT TTCCCCCTCG TGGAAATGCA CATGCAGCTC TTCCAGAATT 
     4141 CATATTACCA GTTTGGGATC AAGTTGCTGT CTGCAGTACC TGGTGGGGAG CGAAAAGTCC 
     4201 TCATCATCTT CAATGCCCCC AGCCTCCAGG ACCGGCTGCG CTTTACATCC GACCTGCGCG 
     4261 AGTCCATTGC GGAGGTGCAG GAGATGGAGA AATACCGTGT GGAGTCGGAG CTGGAGAAGC 
     4321 AGAAAGGTAT GATGCGGCCT AACGCCTCAC AGCCTGGAGG GGCCAAGGAC TCAGTGAATG 
     4381 GGACGATGGC CCGCAGTAGC CTGGAGGACA CTTACGGGGC AGGCGATGGG CTCAAACGGG 
     4441 GCGCACTCAG CAGTTCCCTG CGAGACCTCT CTGATGCAGG GAAGCGGGGG CGGCGTAACA 
     4501 GCGTGGGATC GCTGGACAGC ACCATCGAAG GGTCTGTTAT TAGCAGTCCA CGCCCTCACC 
     4561 AGAGGATGCC ACCTCCGCCC CCACCCCCGC CGCCAGAGGA GTACAAGAGC CAGAGGCCCG 
     4621 TCTCCAACTC CTCATCCTTC CTGGGCTCCC TATTTGGAAG CAAGCGGGGC AAGGGGCCCT 
     4681 TCCAGATGCC ACCACCGCCA ACAGGCCAGG CCTCTGCCTC CTCTTCATCT GCTTCTTCCA 
     4741 CGCACCACCA CCACCACCAC CACCATCATG GCCATAGCCA CGGTGGCCTG GGGGTGCTGC 
     4801 CTGATGGGCA GTCCAAGCTC CAGGCCCTGC ATGCCCAGTA TTGCCAAGGA CCGGGCCCTG 
     4861 CCCCGCCACC CTACCTCCCA CCCCAGCAGC CCTCTCTTCC CCCACCTCCC CAGCAGCCCC 
     4921 CACCCTTGCC CCAGCTGGGC TCCATTCCAC CGCCTCCCGC CTCAGCCCCA CCTGTGGGGC 
     4981 CACATCGCCA CTTCCACGCC CATGGCCCAG TCCCAGGGCC CCAACACTAT ACCTTGGGCC 
     5041 GGCCAGGCAG GGCACCCAGA CGGGGGGCTG GAGGACACCC TCAGTTTGCT CCACATGGCC 
     5101 GCCACCCCCT GCACCAGCCC ACATCCCCAC TGCCCCTGTA CAGTCCTGCC CCCCAGCACC 
     5161 CTCCAGCCCA CAAACAGGGC CCTAAGCACT TCATCTTCAG CCACCACCCA CAGATGATGC 
     5221 CAGCAGCAGG CGCGGCTGGG GGCCCTGGAT CCCGGCCACC AGGGGGCTCC TACTCCCACC 
     5281 CCCACCACCC CCAGTCACCA TTGTCACCAC ACTCACCCAT CCCACCCCAC CCCTCCTATC 
     5341 CACCCCTCCC CCCACCCTCC CCTCACACCC CGCACTCACC CCTTCCACCC ACCTCCCCCC 
     5401 ATGGCCCGCT GCACGCCTCT GGGCCCCCTG GCACAGCCAA CCCCCCCAGT GCAAACCCCA 
     5461 AGGCCAAGCC AAGCCGGATC AGCACCGTGG TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5521 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5581 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5641 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5701 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5761 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5821 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5881 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5941 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     6001 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     6061 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     6121 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     6181 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6241 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6301 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6361 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6421 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6481 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6541 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6601 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6661 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6721 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6781 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6841 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6901 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6961 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     7021 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     7081 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     7141 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     7201 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7261 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7321 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7381 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7441 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7501 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7561 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7621 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7681 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7741 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7801 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7861 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7921 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7981 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     8041 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     8101 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     8161 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     8221 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8281 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8341 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8401 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8461 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8521 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8581 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8761 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8821 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8881 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8941 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     9001 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     9061 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     9121 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     9181 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9241 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9301 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//