LOCUS       RC226393                9105 bp    DNA     circular  SYN 
DEFINITION  MET (Myc-DDK-tagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 1
ACCESSION   NM_001127500
VERSION     NM_001127500.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5262..5353 
                     /label="Myc-DDK"  
     misc_feature    5383..5968 
                     /label="PolyA signal"  
     misc_feature    5440..5459 
                     /label="Restriction Enzyme"  
     misc_feature    6244..6916 
                     /label="ColE1"  
     misc_feature    7346..8140 
                     /label="Kan/Neo"  
     misc_feature    8175..8477 
                     /label="SV40 ori"  
     misc_feature    8563..9040 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5244..5250 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5244 
                     /label="MET"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGCCCCC GCTGTGCTTG CACCTGGCAT CCTCGTGCTC CTGTTTACCT 
     1081 TGGTGCAGAG GAGCAATGGG GAGTGTAAAG AGGCACTAGC AAAGTCCGAG ATGAATGTGA 
     1141 ATATGAAGTA TCAGCTTCCC AACTTCACCG CGGAAACACC CATCCAGAAT GTCATTCTAC 
     1201 ATGAGCATCA CATTTTCCTT GGTGCCACTA ACTACATTTA TGTTTTAAAT GAGGAAGACC 
     1261 TTCAGAAGGT TGCTGAGTAC AAGACTGGGC CTGTGCTGGA ACACCCAGAT TGTTTCCCAT 
     1321 GTCAGGACTG CAGCAGCAAA GCCAATTTAT CAGGAGGTGT TTGGAAAGAT AACATCAACA 
     1381 TGGCTCTAGT TGTCGACACC TACTATGATG ATCAACTCAT TAGCTGTGGC AGCGTCAACA 
     1441 GAGGGACCTG CCAGCGACAT GTCTTTCCCC ACAATCATAC TGCTGACATA CAGTCGGAGG 
     1501 TTCACTGCAT ATTCTCCCCA CAGATAGAAG AGCCCAGCCA GTGTCCTGAC TGTGTGGTGA 
     1561 GCGCCCTGGG AGCCAAAGTC CTTTCATCTG TAAAGGACCG GTTCATCAAC TTCTTTGTAG 
     1621 GCAATACCAT AAATTCTTCT TATTTCCCAG ATCATCCATT GCATTCGATA TCAGTGAGAA 
     1681 GGCTAAAGGA AACGAAAGAT GGTTTTATGT TTTTGACGGA CCAGTCCTAC ATTGATGTTT 
     1741 TACCTGAGTT CAGAGATTCT TACCCCATTA AGTATGTCCA TGCCTTTGAA AGCAACAATT 
     1801 TTATTTACTT CTTGACGGTC CAAAGGGAAA CTCTAGATGC TCAGACTTTT CACACAAGAA 
     1861 TAATCAGGTT CTGTTCCATA AACTCTGGAT TGCATTCCTA CATGGAAATG CCTCTGGAGT 
     1921 GTATTCTCAC AGAAAAGAGA AAAAAGAGAT CCACAAAGAA GGAAGTGTTT AATATACTTC 
     1981 AGGCTGCGTA TGTCAGCAAG CCTGGGGCCC AGCTTGCTAG ACAAATAGGA GCCAGCCTGA 
     2041 ATGATGACAT TCTTTTCGGG GTGTTCGCAC AAAGCAAGCC AGATTCTGCC GAACCAATGG 
     2101 ATCGATCTGC CATGTGTGCA TTCCCTATCA AATATGTCAA CGACTTCTTC AACAAGATCG 
     2161 TCAACAAAAA CAATGTGAGA TGTCTCCAGC ATTTTTACGG ACCCAATCAT GAGCACTGCT 
     2221 TTAATAGGAC ACTTCTGAGA AATTCATCAG GCTGTGAAGC GCGCCGTGAT GAATATCGAA 
     2281 CAGAGTTTAC CACAGCTTTG CAGCGCGTTG ACTTATTCAT GGGTCAATTC AGCGAAGTCC 
     2341 TCTTAACATC TATATCCACC TTCATTAAAG GAGACCTCAC CATAGCTAAT CTTGGGACAT 
     2401 CAGAGGGTCG CTTCATGCAG GTTGTGGTTT CTCGATCAGG ACCATCAACC CCTCATGTGA 
     2461 ATTTTCTCCT GGACTCCCAT CCAGTGTCTC CAGAAGTGAT TGTGGAGCAT ACATTAAACC 
     2521 AAAATGGCTA CACACTGGTT ATCACTGGGA AGAAGATCAC GAAGATCCCA TTGAATGGCT 
     2581 TGGGCTGCAG ACATTTCCAG TCCTGCAGTC AATGCCTCTC TGCCCCACCC TTTGTTCAGT 
     2641 GTGGCTGGTG CCACGACAAA TGTGTGCGAT CGGAGGAATG CCTGAGCGGG ACATGGACTC 
     2701 AACAGATCTG TCTGCCTGCA ATCTACAAGG TTTTCCCAAA TAGTGCACCC CTTGAAGGAG 
     2761 GGACAAGGCT GACCATATGT GGCTGGGACT TTGGATTTCG GAGGAATAAT AAATTTGATT 
     2821 TAAAGAAAAC TAGAGTTCTC CTTGGAAATG AGAGCTGCAC CTTGACTTTA AGTGAGAGCA 
     2881 CGATGAATAC ATTGAAATGC ACAGTTGGTC CTGCCATGAA TAAGCATTTC AATATGTCCA 
     2941 TAATTATTTC AAATGGCCAC GGGACAACAC AATACAGTAC ATTCTCCTAT GTGGATCCTG 
     3001 TAATAACAAG TATTTCGCCG AAATACGGTC CTATGGCTGG TGGCACTTTA CTTACTTTAA 
     3061 CTGGAAATTA CCTAAACAGT GGGAATTCTA GACACATTTC AATTGGTGGA AAAACATGTA 
     3121 CTTTAAAAAG TGTGTCAAAC AGTATTCTTG AATGTTATAC CCCAGCCCAA ACCATTTCAA 
     3181 CTGAGTTTGC TGTTAAATTG AAAATTGACT TAGCCAACCG AGAGACAAGC ATCTTCAGTT 
     3241 ACCGTGAAGA TCCCATTGTC TATGAAATTC ATCCAACCAA ATCTTTTATT AGTACTTGGT 
     3301 GGAAAGAACC TCTCAACATT GTCAGTTTTC TATTTTGCTT TGCCAGTGGT GGGAGCACAA 
     3361 TAACAGGTGT TGGGAAAAAC CTGAATTCAG TTAGTGTCCC GAGAATGGTC ATAAATGTGC 
     3421 ATGAAGCAGG AAGGAACTTT ACAGTGGCAT GTCAACATCG CTCTAATTCA GAGATAATCT 
     3481 GTTGTACCAC TCCTTCCCTG CAACAGCTGA ATCTGCAACT CCCCCTGAAA ACCAAAGCCT 
     3541 TTTTCATGTT AGATGGGATC CTTTCCAAAT ACTTTGATCT CATTTATGTA CATAATCCTG 
     3601 TGTTTAAGCC TTTTGAAAAG CCAGTGATGA TCTCAATGGG CAATGAAAAT GTACTGGAAA 
     3661 TTAAGGGAAA TGATATTGAC CCTGAAGCAG TTAAAGGTGA AGTGTTAAAA GTTGGAAATA 
     3721 AGAGCTGTGA GAATATACAC TTACATTCTG AAGCCGTTTT ATGCACGGTC CCCAATGACC 
     3781 TGCTGAAATT GAACAGCGAG CTAAATATAG AGTGGAAGCA AGCAATTTCT TCAACCGTCC 
     3841 TTGGAAAAGT AATAGTTCAA CCAGATCAGA ATTTCACAGG ATTGATTGCT GGTGTTGTCT 
     3901 CAATATCAAC AGCACTGTTA TTACTACTTG GGTTTTTCCT GTGGCTGAAA AAGAGAAAGC 
     3961 AAATTAAAGA TCTGGGCAGT GAATTAGTTC GCTACGATGC AAGAGTACAC ACTCCTCATT 
     4021 TGGATAGGCT TGTAAGTGCC CGAAGTGTAA GCCCAACTAC AGAAATGGTT TCAAATGAAT 
     4081 CTGTAGACTA CCGAGCTACT TTTCCAGAAG ATCAGTTTCC TAATTCATCT CAGAACGGTT 
     4141 CATGCCGACA AGTGCAGTAT CCTCTGACAG ACATGTCCCC CATCCTAACT AGTGGGGACT 
     4201 CTGATATATC CAGTCCATTA CTGCAAAATA CTGTCCACAT TGACCTCAGT GCTCTAAATC 
     4261 CAGAGCTGGT CCAGGCAGTG CAGCATGTAG TGATTGGGCC CAGTAGCCTG ATTGTGCATT 
     4321 TCAATGAAGT CATAGGAAGA GGGCATTTTG GTTGTGTATA TCATGGGACT TTGTTGGACA 
     4381 ATGATGGCAA GAAAATTCAC TGTGCTGTGA AATCCTTGAA CAGAATCACT GACATAGGAG 
     4441 AAGTTTCCCA ATTTCTGACC GAGGGAATCA TCATGAAAGA TTTTAGTCAT CCCAATGTCC 
     4501 TCTCGCTCCT GGGAATCTGC CTGCGAAGTG AAGGGTCTCC GCTGGTGGTC CTACCATACA 
     4561 TGAAACATGG AGATCTTCGA AATTTCATTC GAAATGAGAC TCATAATCCA ACTGTAAAAG 
     4621 ATCTTATTGG CTTTGGTCTT CAAGTAGCCA AAGGCATGAA ATATCTTGCA AGCAAAAAGT 
     4681 TTGTCCACAG AGACTTGGCT GCAAGAAACT GTATGCTGGA TGAAAAATTC ACAGTCAAGG 
     4741 TTGCTGATTT TGGTCTTGCC AGAGACATGT ATGATAAAGA ATACTATAGT GTACACAACA 
     4801 AAACAGGTGC AAAGCTGCCA GTGAAGTGGA TGGCTTTGGA AAGTCTGCAA ACTCAAAAGT 
     4861 TTACCACCAA GTCAGATGTG TGGTCCTTTG GCGTGCTCCT CTGGGAGCTG ATGACAAGAG 
     4921 GAGCCCCACC TTATCCTGAC GTAAACACCT TTGATATAAC TGTTTACTTG TTGCAAGGGA 
     4981 GAAGACTCCT ACAACCCGAA TACTGCCCAG ACCCCTTATA TGAAGTAATG CTAAAATGCT 
     5041 GGCACCCTAA AGCCGAAATG CGCCCATCCT TTTCTGAACT GGTGTCCCGG ATATCAGCGA 
     5101 TCTTCTCTAC TTTCATTGGG GAGCACTATG TCCATGTGAA CGCTACTTAT GTGAACGTAA 
     5161 AATGTGTCGC TCCGTATCCT TCTCTGTTGT CATCAGAAGA TAACGCTGAT GATGAGGTGG 
     5221 ACACACGACC AGCCTCCTTC TGGGAGACAT CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5281 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5341 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5401 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5461 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5521 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5581 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5641 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5701 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5761 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5821 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5881 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5941 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6001 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6061 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6901 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6961 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7021 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7081 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7141 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7201 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7261 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7321 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7381 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7441 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7501 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7561 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7621 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7681 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7741 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7801 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7861 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7921 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7981 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8041 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8101 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8161 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8221 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8281 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8341 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8461 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8521 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8581 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8641 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8701 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8761 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8821 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8881 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8941 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9001 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9061 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//