LOCUS       RC226391                9087 bp    DNA     circular  SYN 
DEFINITION  SGK223 (Myc-DDK-tagged)-Human homolog of rat pragma of Rnd2 (SGK223)
ACCESSION   NM_001080826
VERSION     NM_001080826.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5244..5335 
                     /label="Myc-DDK"  
     misc_feature    5365..5950 
                     /label="PolyA signal"  
     misc_feature    5422..5441 
                     /label="Restriction Enzyme"  
     misc_feature    6226..6898 
                     /label="ColE1"  
     misc_feature    7328..8122 
                     /label="Kan/Neo"  
     misc_feature    8157..8459 
                     /label="SV40 ori"  
     misc_feature    8545..9022 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5226..5232 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5226 
                     /label="SGK223"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAGACC CTCTGCCTGA ACCCCGAGAG CCTGAAAATG TCTGCGTGCA 
     1081 GTGACTTTGT GGAGCACATC TGGAAACCCG GGTCCTGCAA GAACTGCTTC TGCCTGCGGA 
     1141 GCGACCACCA GCTGGTGGCC GGCCCTCCCC AGCCCAGAGC GGGCAGCCTG CCCCCTCCAC 
     1201 CGCGCCTGCC TCCCAGGCCT GAGAACTGCC GCCTGGAAGA TGAAGGTGTG AACAGCTCAC 
     1261 CTTACTCCAA GCCCACAATT GCCGTGAAGC CCACCATGAT GAGCTCCGAG GCCTCTGATG 
     1321 TGTGGACAGA GGCCAACCTG AGTGCCGAAG TCTCGCAGGT CATCTGGAGA CGAGCCCCTG 
     1381 GCAAGCTCCC CCTCCCGAAG CAGGAGGATG CCCCCGTCGT CTACCTGGGC AGCTTCCGAG 
     1441 GTGTACAGAA GCCTGCTGGT CCCTCTACCT CCCCTGATGG CAATTCTCGC TGTCCCCCAG 
     1501 CTTACACCAT GGTCGGCCTG CACAACCTTG AGCCCCGCGG CGAGAGGAAC ATTGCCTTCC 
     1561 ACCCGGTGAG CTTCCCGGAG GAGAAGGCTG TGCACAAAGA AAAACCCTCA TTTCCTTACC 
     1621 AAGACCGGCC CTCCACCCAG GAGAGCTTCC GCCAGAAACT GGCTGCCTTT GCTGGGACCA 
     1681 CATCTGGCTG TCACCAGGGC CCTGGGCCCC TGCGGGAATC CCTGCCCTCG GAGGATGACA 
     1741 GTGATCAAAG GTGCTCGCCC TCCGGGGACA GCGAGGGTGG AGAGTACTGC TCCATCCTGG 
     1801 ACTGCTGCCC TGGGAGCCCT GTTGCCAAGG CTGCCTCCCA GACTGCAGGT TCCCGGGGCA 
     1861 GGCATGGTGG CAGGGACTGC TCACCCACGT GCTGGGAGCA GGGGAAGTGT TCCGGGCCCG 
     1921 CAGAGCAGGA GAAGCGGGGC CCGAGCTTCC CCAAGGAGTG CTGTAGCCAG GGCCCCACTG 
     1981 CCCACCCATC CTGCCTGGGC CCCAAGAAAC TGTCCCTCAC CTCGGAGGCT GCCATTTCTT 
     2041 CCGACGGCCT CTCTTGTGGC AGCGGCAGCG GCAGCGGCGC CAGTAGCCCC TTCGTCCCCC 
     2101 ACCTCGAGAG TGATTACTGC TCCCTCATGA AGGAACCTGC CCCAGAGAAG CAGCAGGACC 
     2161 CTGGCTGCCC AGGGGTGACC CCTAGCAGAT GCCTTGGGCT GACGGGGGAG CCCCAGCCCC 
     2221 CGGCCCACCC CCGGGAGGCT ACACAGCCTG AACCCATCTA TGCTGAGAGC ACCAAGAGGA 
     2281 AGAAGGCAGC TCCGGTGCCT TCCAAGTCAC AGGCCAAGAT AGAACATGCA GCTGCTGCCC 
     2341 AGGGCCAAGG CCAGGTATGC ACAGGTAATG CCTGGGCCCA GAAAGCAGCA TCTGGCTGGG 
     2401 GCCGGGACAG CCCAGACCCA ACTCCCCAGG TGTCAGCCAC CATCACAGTC ATGGCGGCCC 
     2461 ACCCGGAAGA GGACCATCGG ACGATCTACC TGAGCAGCCC TGACTCTGCA GTGGGGGTGC 
     2521 AGTGGCCACG AGGGCCTGTG AGCCAGAACT CCGAGGTAGG TGAAGAGGAG ACTTCGGCTG 
     2581 GGCAGGGGCT GAGCTCCAGG GAAAGCCATG CTCACAGTGC CAGCGAGAGC AAGCCCAAGG 
     2641 AGAGGCCCGC CATTCCCCCC AAGTTGTCCA AGAGTAGCCC TGTAGGGTCC CCGGTGTCAC 
     2701 CGTCTGCTGG AGGGCCCCCA GTGTCACCGC TGGCTGACCT TAGTGATGGG AGCTCTGGCG 
     2761 GCAGCAGCAT TGGGCCCCAG CCTCCATCCC AAGGTCCTGC TGACCCCGCT CCTTCCTGCC 
     2821 GGACCAACGG TGTCGCTATC AGTGACCCAT CCAGGTGTCC CCAGCCTGCC GCCTCGTCAG 
     2881 CCTCGGAACA GAGGCGGCCC AGGTTCCAGG CAGGCACCTG GAGTCGTCAG TGCCGGATAG 
     2941 AGGAAGAAGA GGAGGTGGAG CAGGAATTGC TGAGTCACAG CTGGGGAAGA GAGACCAAAA 
     3001 ATGGCCCCAC GGACCATTCA AACTCCACGA CCTGGCACCG TCTCCACCCC ACAGATGGCT 
     3061 CCTCTGGGCA GAACAGCAAA GTTGGGACCG GGATGAGCAA ATCCGCCTCT TTTGCCTTTG 
     3121 AGTTCCCCAA GGACAGAAGT GGGATTGAGA CATTCTCACC TCCTCCTCCG CCTCCAAAGT 
     3181 CGCGGCACCT TCTAAAAATG AACAAGAGCA GCTCTGATTT GGAAAAAGTG AGCCAGGGCT 
     3241 CTGCAGAAAG CCTCAGCCCA TCCTTCAGGG GTGTCCACGT CAGCTTCACC ACCGGCTCCA 
     3301 CGGACAGCCT GGCCTCAGAC TCTAGGACCT GCAGCGATGG AGGTCCCTCG TCTGAGCTGG 
     3361 CTCACTCGCC CACCAACAGC GGGAAGAAGC TCTTTGCTCC CGTTCCGTTT CCTTCAGGCT 
     3421 CCACTGAGGA CGTGTCCCCC AGTGGCCCCC AGCAGCCCCC TCCACTCCCC CAGAAAAAGA 
     3481 TAGTGAGCCG GGCAGCCTCT TCACCGGATG GCTTCTTCTG GACCCAAGGC TCCCCCAAGC 
     3541 CCGGAACAGC AAGCCCCAAG CTGAACCTAA GCCACTCGGA AACCAACGTC CACGACGAAT 
     3601 CTCACTTTAG CTATTCGTTG AGCCCCGGGA ACCGCCACCA TCCTGTCTTC TCCTCTTCCG 
     3661 ATCCTCTGGA GAAAGCTTTC AAAGGCAGTG GCCACTGGCT TCCGGCAGCA GGGCTGGCGG 
     3721 GCAACAGAGG CGGCTGCGGG AGCCCTGGCC TCCAGTGCAA AGGGGCCCCC TCCGCCTCAT 
     3781 CCTCCCAGCT GAGCGTGTCC AGTCAAGCCT CCACCGGGAG CACCCAGCTT CAGCTGCACG 
     3841 GTCTCCTGAG CAACATCAGC AGCAAGGAGG GCACCTATGC CAAGCTGGGG GGACTCTACA 
     3901 CCCAGTCCCT GGCCCGCCTT GTAGCCAAAT GTGAGGACCT CTTCATGGGC GGCCAGAAAA 
     3961 AGGAGCTCCA CTTCAATGAG AATAACTGGT CGCTCTTCAA GCTGACTTGT AACAAGCCCT 
     4021 GCTGTGACTC GGGGGATGCC ATTTATTACT GTGCCACCTG CTCTGAGGAC CCCGGCAGCA 
     4081 CCTATGCTGT GAAAATCTGC AAAGCCCCTG AGCCCAAAAC AGTCTCCTAC TGCAGCCCGT 
     4141 CCGTGCCCGT GCACTTTAAC ATCCAGCAGG ACTGCGGCCA CTTCGTCGCC TCGGTGCCGT 
     4201 CCAGCATGCT CAGCTCCCCC GACGCGCCCA AGGACCCTGT GCCTGCCCTG CCCACACACC 
     4261 CCCCTGCCCA GGAGCAGGAC TGCGTGGTGG TCATCACCCG AGAGGTGCCA CATCAGACCG 
     4321 CCTCCGACTT CGTGCGGGAC TCGGCGGCCA GCCACCAGGC GGAGCCCGAG GCGTACGAGC 
     4381 GGCGCGTGTG CTTCCTGCTT CTGCAACTCT GCAACGGGCT GGAGCACCTG AAGGAGCACG 
     4441 GGATCATCCA CCGGGACCTG TGCCTGGAGA ACCTGCTGCT GGTGCACTGC ACCCTCCAGG 
     4501 CCGGCCCCGG GCCCGCCCCC GCCCCGGCTC CCGCCCCCGC CGCCGCCGCG CCTCCCTGCT 
     4561 CCTCTGCCGC CCCGCCTGCT GGTGGCACTC TCAGCCCCGC AGCCGGCCCC GCCTCCCCGG 
     4621 AAGGGCCCCG GGAGAAGCAG CTGCCCCGGC TCATCATCAG CAACTTTTTG AAGGCCAAGC 
     4681 AGAAGCCGGG CGGCACCCCA AACCTGCAGC AGAAGAAGAG CCAGGCCCGG CTGGCCCCCG 
     4741 AGATCGTGTC TGCTTCCCAG TACCGCAAGT TCGATGAGTT CCAGACAGGC ATCCTCATCT 
     4801 ACGAGCTGCT GCACCAACCC AACCCGTTCG AGGTGCGCGC CCAGCTGCGG GAGAGAGACT 
     4861 ACCGGCAGGA GGACCTGCCG CCGCTGCCCG CGCTGTCCCT CTACTCACCC GGCCTGCAGC 
     4921 AGCTGGCACA TCTGCTACTG GAGGCCGACC CCATCAAGCG TATCCGCATC GGCGAGGCCA 
     4981 AGCGCGTGCT GCAGTGCCTG CTGTGGGGGC CTCGGCGCGA GCTGGTGCAG CAGCCGGGCA 
     5041 CCTCGGAGGA GGCGCTGTGC GGCACGCTGC ACAACTGGAT CGACATGAAG CGGGCCCTGA 
     5101 TGATGATGAA GTTTGCGGAG AAGGCGGTGG ATCGCAGGCG GGGCGTGGAG CTGGAGGACT 
     5161 GGCTTTGCTG CCAGTACCTG GCGTCTGCGG AGCCCGGGGC CCTCTTACAG TCGCTGAAGC 
     5221 TCCTGCAGCT TCTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5281 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5341 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5401 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5461 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5521 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5581 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5641 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5701 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5761 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5821 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5881 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5941 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6001 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6061 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6121 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6181 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6241 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6301 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6361 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6421 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6481 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6541 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6601 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6661 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6721 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6781 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6841 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6901 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6961 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7021 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7081 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7141 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7201 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7261 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7321 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7381 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7441 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7501 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7561 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7621 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7681 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7741 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7801 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7861 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7921 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7981 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8041 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8101 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8161 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8221 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8281 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8341 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8401 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8461 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8521 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8581 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8641 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8701 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8761 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8821 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8881 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8941 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9001 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9061 ATTTAACAAA AATTTAACGC GAATTTT
//