LOCUS       RC226384                8865 bp    DNA     circular  SYN 
DEFINITION  FANCI (Myc-DDK-tagged)-Human Fanconi anemia, complementation group I (FANCI), transcript variant 1
ACCESSION   NM_001113378
VERSION     NM_001113378.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5022..5113 
                     /label="Myc-DDK"  
     misc_feature    5143..5728 
                     /label="PolyA signal"  
     misc_feature    5200..5219 
                     /label="Restriction Enzyme"  
     misc_feature    6004..6676 
                     /label="ColE1"  
     misc_feature    7106..7900 
                     /label="Kan/Neo"  
     misc_feature    7935..8237 
                     /label="SV40 ori"  
     misc_feature    8323..8800 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5004..5010 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5004 
                     /label="FANCI"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCAGAAG ATTTTATCTC TAGCAGCAGA AAAAACAGCA GACAAACTGC 
     1081 AAGAATTTCT TCAAACCCTG AGAGAAGGTG ATTTGACTAA TCTCCTTCAG AATCAAGCAG 
     1141 TGAAAGGAAA AGTTGCTGGA GCACTCCTGA GAGCCATCTT CAAAGGTTCC CCCTGCTCTG 
     1201 AGGAAGCTGG AACACTTAGG AGACGTAAGA TATACACTTG TTGTATCCAG TTGGTGGAAT 
     1261 CGGGGGATTT GCAGAAAGAA ATAGCGTCTG AGATCATAGG ATTACTGATG CTGGAGGCTC 
     1321 ACCATTTTCC AGGACCATTA TTGGTTGAAT TAGCCAATGA GTTTATTAGT GCTGTCAGAG 
     1381 AAGGCAGCCT AGTGAATGGA AAATCTTTGG AGTTACTACC TATCATTCTC ACTGCCCTGG 
     1441 CTACGAAAAA GGAAAATCTG GCTTATGGAA AAGGTGTACT GAGTGGGGAA GAATGTAAGA 
     1501 AACAGTTGAT TAACACCCTG TGTTCTGGCA GGTGGGATCA GCAATATGTA ATCCAACTCA 
     1561 CCTCCATGTT CAAGGATGTC CCTCTGACTG CAGAAGAGGT GGAATTTGTG GTGGAAAAAG 
     1621 CATTGAGCAT GTTCTCCAAG ATGAATCTTC AAGAAATACC ACCTTTGGTC TATCAGCTTC 
     1681 TGGTTCTCTC CTCCAAGGGA AGCAGAAAGA GTGTTTTGGA AGGAATCATA GCCTTCTTCA 
     1741 GTGCACTAGA TAAGCAGCAC AATGAGGAAC AGAGTGGTGA CGAGCTATTG GATGTTGTCA 
     1801 CTGTGCCATC AGGTGAACTT CGTCATGTGG AAGGCACCAT TATTCTACAC ATTGTGTTTG 
     1861 CCATCAAATT GGACTATGAA CTAGGCAGAG AACTCGTGAA ACACTTAAAG GTAGGACAGC 
     1921 AAGGAGATTC CAATAATAAC TTAAGTCCCT TCAGCATTGC TCTTCTTCTG TCTGTAACAA 
     1981 GAATACAAAG ATTTCAGGAC CAGGTGCTTG ATCTTTTAAA GACTTCGGTT GTAAAGAGCT 
     2041 TTAAGGATCT TCAACTCCTC CAAGGCTCAA AATTTCTTCA GAATCTAGTT CCTCATAGAT 
     2101 CTTATGTTTC AACCATGATC TTGGAAGTAG TGAAGAATAG CGTTCATAGC TGGGACCATG 
     2161 TTACTCAGGG CCTCGTAGAA CTTGGTTTCA TTTTGATGGA TTCATATGGG CCAAAGAAGG 
     2221 TTCTTGATGG AAAAACTATT GAAACCAGCC CAAGTCTTTC TAGAATGCCA AACCAGCATG 
     2281 CATGTAAGCT CGGAGCTAAT ATCCTGTTGG AAACTTTTAA GATCCATGAG ATGATCAGAC 
     2341 AAGAAATTTT GGAGCAGGTC CTCAACAGGG TTGTTACCAG AGCATCTTCT CCCATCAGTC 
     2401 ATTTCTTAGA CCTGCTTTCA AATATCGTCA TGTATGCACC CTTAGTTCTT CAAAGTTGTT 
     2461 CTTCTAAAGT CACAGAAGCT TTTGACTATT TGTCCTTTCT GCCCCTTCAG ACTGTACAAA 
     2521 GGCTGCTTAA GGCAGTGCAG CCCCTTCTCA AAGTCAGCAT GTCAATGAGA GACTGCTTGA 
     2581 TACTTGTCCT TCGGAAAGCT ATGTTTGCCA ACCAGCTTGA TGCCCGAAAA TCTGCAGTTG 
     2641 CTGGGTTTTT GCTGCTCCTG AAGAACTTTA AAGTTTTAGG CAGCCTGTCA TCCTCTCAGT 
     2701 GCAGTCAGTC TCTCAGTGTC AGTCAGGTTC ATGTGGATGT TCACAGCCAT TACAATTCTG 
     2761 TCGCCAATGA AACTTTTTGC CTTGAGATCA TGGATAGTTT GAGGAGATGC TTAAGCCAGC 
     2821 AAGCTGATGT TCGACTCATG CTTTATGAGG GGTTTTATGA TGTTCTTCGA AGGAACTCTC 
     2881 AGCTGGCTAA TTCAGTCATG CAAACTCTGC TCTCACAGTT AAAACAGTTC TATGAGCCAA 
     2941 AACCTGATCT GCTGCCTCCT CTGAAATTAG AAGCTTGTAT TCTGACCCAA GGAGATAAGA 
     3001 TCTCTCTACA AGAACCACTG GATTATCTGC TGTGTTGTAT TCAGCATTGT TTGGCCTGGT 
     3061 ATAAGAATAC AGTCATACCC TTACAGCAGG GAGAGGAGGA AGAGGAGGAG GAAGAGGCAT 
     3121 TCTACGAAGA CCTAGATGAT ATATTGGAGT CCATTACTAA TAGAATGATT AAGAGTGAGC 
     3181 TGGAAGACTT TGAACTGGAT AAATCAGCAG ATTTTTCTCA GAGCACCAGT ATTGGCATAA 
     3241 AAAATAATAT CTGTGCTTTT CTTGTGATGG GAGTTTGTGA GGTTTTAATA GAATACAATT 
     3301 TCTCCATAAG TAGTTTCAGT AAGAATAGGT TTGAGGACAT TCTGAGCTTA TTTATGTGTT 
     3361 ACAAAAAACT CTCTGACATT CTTAATGAAA AAGCGGGTAA AGCCAAAACT AAAATGGCCA 
     3421 ACAAGACAAG TGATAGTCTT TTGTCCATGA AATTTGTGTC CAGTCTTCTC ACTGCTCTTT 
     3481 TCAGGGATAG TATCCAAAGC CACCAAGAAA GCCTTTCTGT TCTCAGGTCC AGCAATGAGT 
     3541 TTATGCGCTA TGCAGTGAAT GTAGCTCTGC AGAAAGTACA GCAGCTAAAG GAAACAGGGC 
     3601 ATGTGAGTGG CCCTGATGGC CAAAACCCAG AAAAGATCTT TCAGAACCTC TGTGACATAA 
     3661 CTCGAGTCTT GCTATGGAGA TACACTTCAA TTCCTACTTC AGTGGAAGAG TCGGGAAAGA 
     3721 AAGAGAAAGG AAAGAGCATC TCACTGCTGT GCTTGGAGGG TTTACAGAAA ATATTCAGTG 
     3781 CTGTGCAACA GTTCTATCAG CCCAAGATTC AGCAGTTTCT CAGAGCTCTG GATGTCACAG 
     3841 ATAAGGAAGG AGAAGAGAGA GAAGATGCAG ATGTCAGTGT CACTCAGAGA ACAGCATTCC 
     3901 AGATCCGGCA ATTTCAGAGG TCCTTGTTGA ATTTACTTAG CAGTCAAGAG GAAGATTTTA 
     3961 ATAGCAAAGA AGCCCTCCTG CTAGTCACGG TTCTTACCAG TTTGTCCAAG TTACTGGAGC 
     4021 CCTCCTCTCC TCAGTTTGTG CAGATGTTAT CCTGGACATC AAAGATTTGC AAGGAAAACA 
     4081 GCCGGGAGGA TGCCTTGTTT TGCAAGAGCT TGATGAACTT GCTCTTCAGC CTGCATGTTT 
     4141 CGTATAAGAG TCCTGTCATT CTGCTGCGTG ACTTGTCCCA GGATATCCAC GGGCATCTGG 
     4201 GAGATATAGA CCAGGATGTA GAGGTGGAGA AAACAAACCA CTTTGCAATA GTGAATTTGA 
     4261 GAACGGCTGC CCCCACTGTC TGTTTACTTG TTCTGAGTCA GGCCGAGAAG GTTCTAGAAG 
     4321 AAGTGGACTG GCTAATCACC AAGCTTAAGG GACAAGTGAG CCAAGAAACC TTATCAGAAG 
     4381 AGGCCTCTTC TCAGGCAACC CTACCAAATC AGCCTGTTGA GAAAGCTATC ATCATGCAAC 
     4441 TGGGAACTCT GCTTACATTT TTCCACGAGC TGGTGCAGAC AGCTCTGCCA TCAGGCAGCT 
     4501 GTGTGGACAC CTTGTTAAAG GACTTGTGCA AAATGTACAC CACACTTACA GCCCTTGTCA 
     4561 GATATTATCT CCAGGTGTGT CAGAGCTCCG GAGGAATTCC AAAAAATATG GAAAAGCTGG 
     4621 TGAAGCTGTC TGGTTCTCAT CTGACCCCCC TGTGTTATTC TTTCATTTCT TACGTACAGA 
     4681 ATAAGAGTAA GAGCCTGAAC TATACGGGAG AGAAAAAGGA GAAACCTGCT GCCGTTGCCA 
     4741 CAGCCATGGC CAGAGTTCTT CGGGAAACCA AGCCAATCCC TAACCTCATC TTTGCCATAG 
     4801 AACAGTATGA AAAATTTCTC ATCCACCTTT CTAAGAAGTC CAAGGTGAAC CTGATGCAGC 
     4861 ACATGAAGCT CAGCACCTCA CGAGACTTCA AGATCAAAGG AAACATCCTA GACATGGTTC 
     4921 TTCGAGAGGA TGGTGAAGAT GAAAATGAAG AGGGCACTGC ATCAGAGCAT GGGGGACAGA 
     4981 ACAAAGAACC AGCCAAGAAG AAAAGGAAAA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5041 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5101 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5161 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5221 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5281 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5341 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5401 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5461 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5521 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5581 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5641 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5701 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5761 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5821 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5881 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5941 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6001 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6061 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6361 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6601 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6661 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6721 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6781 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6841 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6901 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6961 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7021 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7081 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7141 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7201 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7261 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7321 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7381 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7441 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7501 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7561 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7621 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7681 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7741 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7801 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7861 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7921 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7981 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8041 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8101 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8281 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8341 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8401 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8461 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8521 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8581 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8641 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8701 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8761 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8821 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//