LOCUS       RC226382                8844 bp    DNA     circular  SYN 
DEFINITION  SPAG9 (Myc-DDK-tagged)-Human sperm associated antigen 9 (SPAG9), transcript variant 1
ACCESSION   NM_001130528
VERSION     NM_001130528.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5001..5092 
                     /label="Myc-DDK"  
     misc_feature    5122..5707 
                     /label="PolyA signal"  
     misc_feature    5179..5198 
                     /label="Restriction Enzyme"  
     misc_feature    5983..6655 
                     /label="ColE1"  
     misc_feature    7085..7879 
                     /label="Kan/Neo"  
     misc_feature    7914..8216 
                     /label="SV40 ori"  
     misc_feature    8302..8779 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4983..4989 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4983 
                     /label="SPAG9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTGGAG GACGGTGTGG TGTATCAGGA GGAGCCCGGC GGCTCCGGGG 
     1081 CCGTGATGTC GGAGCGGGTG TCCGGCCTGG CCGGCTCCAT CTACCGCGAG TTCGAGCGGC 
     1141 TTATCGGGCG CTATGACGAG GAGGTGGTCA AAGAGCTGAT GCCGCTGGTG GTGGCTGTGC 
     1201 TGGAGAACCT GGACTCGGTG TTCGCGCAGG ACCAGGAGCA CCAGGTGGAG CTGGAGCTGC 
     1261 TGCGGGACGA CAACGAGCAG CTCATCACCC AGTACGAGCG GGAGAAGGCG CTGCGCAAGC 
     1321 ACGCTGAGGA GAAATTCATT GAATTTGAAG ACTCTCAAGA ACAGGAAAAA AAGGACTTAC 
     1381 AGACCCGAGT GGAATCTTTA GAATCTCAAA CAAGACAACT TGAGCTGAAA GCGAAAAACT 
     1441 ATGCTGACCA GATTAGCAGA CTTGAAGAAA GAGAAGCAGA ACTGAAGAAG GAATATAATG 
     1501 CATTACATCA AAGACACACT GAGATGATCC ATAATTATAT GGAACATTTA GAAAGAACAA 
     1561 AACTTCATCA GCTCTCAGGG AGTGATCAAC TAGAATCCAC AGCTCATAGT AGAATTAGAA 
     1621 AAGAACGCCC TATATCATTA GGAATTTTCC CATTACCTGC TGGAGATGGA TTGCTTACAC 
     1681 CTGATGCTCA GAAAGGAGGA GAGACCCCTG GATCTGAGCA ATGGAAATTT CAGGAATTAA 
     1741 GTCAACCACG TTCTCATACC AGCCTGAAGG TCAGCAATAG TCCTGAACCT CAGAAGGCTG 
     1801 TAGAACAGGA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT ACCACTCCAG 
     1861 CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA AAGGAAGAAA 
     1921 ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC AAACACATTG 
     1981 AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA AATGAAGAAA 
     2041 AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC AAAGATCTCA 
     2101 GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT TTTGATCGCA 
     2161 ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA GGAGATGTGG 
     2221 ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA TTAGAAAATA 
     2281 CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG ATAGCAAAAG 
     2341 TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT GTGAAGCAAG 
     2401 CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG AAAGCTCGGG 
     2461 CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT ATTCCCACAG 
     2521 CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG CGAAACCAGT 
     2581 ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG ATTCGGGCAT 
     2641 CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG TTTTTCAGCC 
     2701 GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT AATCTGAAGT 
     2761 ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC ACCTTATCTC 
     2821 AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT GAAGCTAGTT 
     2881 TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA CATGTTCAGA 
     2941 AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC AAACAGGTAA 
     3001 CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT CTCAGACCTC 
     3061 TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT TTATCTGGTG 
     3121 GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG GATGTTGCTG 
     3181 GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA GATAAGTTAG 
     3241 ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA TCCAGTCTAG 
     3301 TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT GCTGTTCAAC 
     3361 CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC ATTGCAAGTG 
     3421 TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA TCTGGTCAGG 
     3481 TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG ACAGACAGCC 
     3541 TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA GCTGCCACTT 
     3601 CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG GAAGCAGAAA 
     3661 ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT ACAGAAGGGA 
     3721 ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC ACAGAGCATG 
     3781 TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG TATCAGTCGA 
     3841 GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA CAAGACTTGG 
     3901 TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT GGAGCTCAAA 
     3961 ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC CATTCCATTA 
     4021 AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA GTAGCCCTGG 
     4081 CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT TTGTCAAACT 
     4141 ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT GTGGTACATG 
     4201 ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG GCCATGAAAA 
     4261 TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG CTTGCGTGGG 
     4321 TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC TATCATGCAC 
     4381 ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA ATGTTAGGTA 
     4441 CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT TGTAATCGTT 
     4501 TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA ACAAATAAAA 
     4561 CCTCAGGTGT ACCAGGAAAT CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAAAACA 
     4621 GTGATAAAGT GACTCCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT 
     4681 GCTTCCATGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAAGTCATCA 
     4741 GCCCACAAAG TAGCAGTAGT GGCACGGATC TGACGGGTGA CAAAGCAGGG CCATCTGCAC 
     4801 AGGAGCCTGG TAGTCAGACG CCCTTGAAGT CTATGCTTGT CATCAGTGGA GGAGAGGGCT 
     4861 ACATCGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA ACTTCTTGGA GAGGATCTTC 
     4921 CACTTGAACC TTCTGTCACC AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT 
     4981 ATGGCAATGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5041 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5101 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5161 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5221 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5281 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5341 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5401 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5461 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5521 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5581 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5641 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5701 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5761 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5821 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5881 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5941 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6001 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6061 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6121 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6181 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6241 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6301 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6361 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6421 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6481 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6541 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6601 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6661 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6721 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6781 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6841 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6901 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6961 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7021 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7081 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7141 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7201 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7261 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7321 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7381 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7441 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7501 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7561 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7621 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7681 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7741 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7801 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7861 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7921 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7981 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8041 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8101 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8161 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8221 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8281 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8341 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8401 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8461 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8521 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8581 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8641 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8701 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8761 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8821 TAACAAAAAT TTAACGCGAA TTTT
//