LOCUS       RC226380                8814 bp    DNA     circular  SYN 
DEFINITION  PRG4 (Myc-DDK-tagged)-Human proteoglycan 4 (PRG4), transcript variant C
ACCESSION   NM_001127709
VERSION     NM_001127709.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="PRG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATGGAAA ACACTTCCCA TTTACCTGTT GTTGCTGCTG TCTGTTTTCG 
     1081 TGATTCAGCA AGTTTCATCT CAAGATTTAT CAAGCTGTGC AGGGAGATGT GGGGAAGGGT 
     1141 ATTCTAGAGA TGCCACCTGC AACTGTGATT ATAACTGTCA ACACTACATG GAGTGCTGCC 
     1201 CTGATTTCAA GAGAGTCTGC ACTGCGGAGC TTTCCTGTAA AGGCCGCTGC TTTGAGTCCT 
     1261 TCGAGAGAGG GAGGGAGTGT GACTGCGACG CCCAATGTAA GAAGTATGAC AAGTGCTGTC 
     1321 CCGATTATGA GAGTTTCTGT GCAGAAGTAA AAGATAACAA GAAGAACAGA ACTAAAAAGA 
     1381 AACCTACCCC CAAACCACCA GTTGTAGATG AAGCTGGAAG TGGATTGGAC AATGGTGACT 
     1441 TCAAGGTCAC AACTCCTGAC ACGTCTACCA CCCAACACAA TAAAGTCAGC ACATCTCCCA 
     1501 AGATCACAAC AGCAAAACCA ATAAATCCCA GACCCAGTCT TCCACCTAAT TCTGATACAT 
     1561 CTAAAGAGAC GTCTTTGACA GTGAATAAAG AGACAACAGT TGAAACTAAA GAAACTACTA 
     1621 CAACAAATAA ACAGACTTCA ACTGATGGAA AAGAGAAGAC TACTTCCGCT AAAGAGACAC 
     1681 AAAGTATAGA GAAAACATCT GCTAAAGATT TAGCACCCAC ATCTAAAGTG CTGGCTAAAC 
     1741 CTACACCCAA AGCTGAAACT ACAACCAAAG GCCCTGCTCT CACCACTCCC AAGGAGCCCA 
     1801 CGCCCACCAC TCCCAAGGAG CCTGCATCTA CCACACCCAA AGAGCCCACA CCTACCACCA 
     1861 TCAAGTCTGC ACCCACCACC CCCAAGGAGC CTGCACCCAC CACCACCAAG TCTGCACCCA 
     1921 CCACTCCCAA GGAGCCTGCA CCCACCACCA CCAAGGAGCC TGCACCCACC ACTCCCAAGG 
     1981 AGCCTGCACC CACCACCACC AAGGAGCCTG CACCCACCAC CACCAAGTCT GCACCCACCA 
     2041 CTCCCAAGGA GCCTGCACCC ACCACCCCCA AGAAGCCTGC CCCAACTACC CCCAAGGAGC 
     2101 CTGCACCCAC CACTCCCAAG GAGCCTACAC CCACCACTCC CAAGGAGCCT GCACCCACCA 
     2161 CCAAGGAGCC TGCACCCACC ACTCCCAAAG AGCCTGCACC CACTGCCCCC AAGAAGCCTG 
     2221 CCCCAACTAC CCCCAAGGAG CCTGCACCCA CCACTCCCAA GGAGCCTGCA CCCACCACCA 
     2281 CCAAGGAGCC TTCACCCACC ACTCCCAAGG AGCCTGCACC CACCACCACC AAGTCTGCAC 
     2341 CCACCACTAC CAAGGAGCCT GCACCCACCA CTACCAAGTC TGCACCCACC ACTCCCAAGG 
     2401 AGCCTTCACC CACCACCACC AAGGAGCCTG CACCCACCAC TCCCAAGGAG CCTGCACCCA 
     2461 CCACCCCCAA GAAGCCTGCC CCAACTACCC CCAAGGAGCC TGCACCCACC ACTCCCAAGG 
     2521 AACCTGCACC CACCACCACC AAGAAGCCTG CACCCACCAC TCCCAAAGAG CCTGCCCCAA 
     2581 CTACCCCCAA GGAGACTGCA CCCACCACCC CCAAGAAGCT CACGCCCACC ACCCCCGAGA 
     2641 AGCTCGCACC CACCACCCCT GAGAAGCCCG CACCCACCAC CCCTGAGGAG CTCGCACCCA 
     2701 CCACCCCTGA GGAGCCCACA CCCACCACCC CTGAGGAGCC TGCTCCCACC ACTCCCAAGG 
     2761 CAGCGGCTCC CAACACCCCT AAGGAGCCTG CTCCAACTAC CCCTAAGGAG CCTGCTCCAA 
     2821 CTACCCCTAA GGAGCCTGCT CCAACTACCC CTAAGGAGAC TGCTCCAACT ACCCCTAAAG 
     2881 GGACTGCTCC AACTACCCTC AAGGAACCTG CACCCACTAC TCCCAAGAAG CCTGCCCCCA 
     2941 AGGAGCTTGC ACCCACCACC ACCAAGGAGC CCACATCCAC CACCTGTGAC AAGCCCGCTC 
     3001 CAACTACCCC TAAGGGGACT GCTCCAACTA CCCCTAAGGA GCCTGCTCCA ACTACCCCTA 
     3061 AGGAGCCTGC TCCAACTACC CCTAAGGGGA CTGCTCCAAC TACCCTCAAG GAACCTGCAC 
     3121 CCACTACTCC CAAGAAGCCT GCCCCCAAGG AGCTTGCACC CACCACCACC AAGGGGCCCA 
     3181 CATCCACCAC CTCTGACAAG CCTGCTCCAA CTACACCTAA GGAGACTGCT CCAACTACCC 
     3241 CCAAGGAGCC TGCACCCACT ACCCCCAAGA AGCCTGCTCC AACTACTCCT GAGACACCTC 
     3301 CTCCAACCAC TTCAGAGGTC TCTACTCCAA CTACCACCAA GGAGCCTACC ACTATCCACA 
     3361 AAAGCCCTGA TGAATCAACT CCTGAGCTTT CTGCAGAACC CACACCAAAA GCTCTTGAAA 
     3421 ACAGTCCCAA GGAACCTGGT GTACCTACAA CTAAGACTCC TGCAGCGACT AAACCTGAAA 
     3481 TGACTACAAC AGCTAAAGAC AAGACAACAG AAAGAGACTT ACGTACTACA CCTGAAACTA 
     3541 CAACTGCTGC ACCTAAGATG ACAAAAGAGA CAGCAACTAC AACAGAAAAA ACTACCGAAT 
     3601 CCAAAATAAC AGCTACAACC ACACAAGTAA CATCTACCAC AACTCAAGAT ACCACACCAT 
     3661 TCAAAATTAC TACTCTTAAA ACAACTACTC TTGCACCCAA AGTAACTACA ACAAAAAAGA 
     3721 CAATTACTAC CACTGAGATT ATGAACAAAC CTGAAGAAAC AGCTAAACCA AAAGACAGAG 
     3781 CTACTAATTC TAAAGCGACA ACTCCTAAAC CTCAAAAGCC AACCAAAGCA CCCAAAAAAC 
     3841 CCACTTCTAC CAAAAAGCCA AAAACAATGC CTAGAGTGAG AAAACCAAAG ACGACACCAA 
     3901 CTCCCCGCAA GATGACATCA ACAATGCCAG AATTGAACCC TACCTCAAGA ATAGCAGAAG 
     3961 CCATGCTCCA AACCACCACC AGACCTAACC AAACTCCAAA CTCCAAACTA GTTGAAGTAA 
     4021 ATCCAAAGAG TGAAGATGCA GGTGGTGCTG AAGGAGAAAC ACCTCATATG CTTCTCAGGC 
     4081 CCCATGTGTT CATGCCTGAA GTTACTCCCG ACATGGATTA CTTACCGAGA GTACCCAATC 
     4141 AAGGCATTAT CATCAATCCC ATGCTTTCCG ATGAGACCAA TATATGCAAT GGTAAGCCAG 
     4201 TAGATGGACT GACTACTTTG CGCAATGGGA CATTAGTTGC ATTCCGAGGT CATTATTTCT 
     4261 GGATGCTAAG TCCATTCAGT CCACCATCTC CAGCTCGCAG AATTACTGAA GTTTGGGGTA 
     4321 TTCCTTCCCC CATTGATACT GTTTTTACTA GGTGCAACTG TGAAGGAAAA ACTTTCTTCT 
     4381 TTAAGGATTC TCAGTACTGG CGTTTTACCA ATGATATAAA AGATGCAGGG TACCCCAAAC 
     4441 CAATTTTCAA AGGATTTGGA GGACTAACTG GACAAATAGT GGCAGCGCTT TCAACAGCTA 
     4501 AATATAAGAA CTGGCCTGAA TCTGTGTATT TTTTCAAGAG AGGTGGCAGC ATTCAGCAGT 
     4561 ATATTTATAA ACAGGAACCT GTACAGAAGT GCCCTGGAAG AAGGCCTGCT CTAAATTATC 
     4621 CAGTGTATGG AGAAACGACA CAGGTTAGGA GACGTCGCTT TGAACGTGCT ATAGGACCTT 
     4681 CTCAAACACA CACCATCAGA ATTCAATATT CACCTGCCAG ACTGGCTTAT CAAGACAAAG 
     4741 GTGTCCTTCA TAATGAAGTT AAAGTGAGTA TACTGTGGAG AGGACTTCCA AATGTGGTTA 
     4801 CCTCAGCTAT ATCACTGCCC AACATCAGAA AACCTGACGG CTATGATTAC TATGCCTTTT 
     4861 CTAAAGATCA ATACTATAAC ATTGATGTGC CTAGTAGAAC AGCAAGAGCA ATTACTACTC 
     4921 GTTCTGGGCA GACCTTATCC AAAGTCTGGT ACAACTGTCC TACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//