LOCUS       RC226379                8790 bp    DNA     circular  SYN 
DEFINITION  LTBP3 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 3 (LTBP3), transcript variant 1
ACCESSION   NM_001130144
VERSION     NM_001130144.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4947..5038 
                     /label="Myc-DDK"  
     misc_feature    5068..5653 
                     /label="PolyA signal"  
     misc_feature    5125..5144 
                     /label="Restriction Enzyme"  
     misc_feature    5929..6601 
                     /label="ColE1"  
     misc_feature    7031..7825 
                     /label="Kan/Neo"  
     misc_feature    7860..8162 
                     /label="SV40 ori"  
     misc_feature    8248..8725 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4929..4935 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4929 
                     /label="LTBP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGGCCC CGAGGGGCTG CTGGCGGCCT GGCCCCTGAG ATGCGCGGGG 
     1081 CGGGGGCGGC GGGGCTGCTG GCGCTGCTGC TGCTGCTGCT GCTGCTGCTG CTGGGCCTGG 
     1141 GCGGCAGGGT CGAGGGGGGG CCGGCCGGCG AGCGGGGCGC AGGCGGGGGC GGGGCGCTGG 
     1201 CCCGCGAGCG CTTCAAGGTG GTCTTTGCGC CGGTGATCTG CAAGCGGACC TGTCTCAAGG 
     1261 GCCAGTGTCG GGACAGTTGT CAGCAGGGCT CCAACATGAC GCTCATCGGA GAGAACGGCC 
     1321 ACAGCACAGA CACGCTCACG GGCTCCGGCT TCCGCGTGGT GGTGTGCCCT CTCCCCTGCA 
     1381 TGAATGGCGG CCAGTGCTCC TCGCGAAACC AGTGCCTGTG TCCCCCGGAC TTCACTGGGC 
     1441 GCTTCTGCCA GGTGCCCGCA GGAGGAGCCG GTGGGGGTAC CGGCGGCTCA GGCCCCGGCC 
     1501 TGAGCAGGAC AGGGGCCCTG TCCACAGGGG CGCTGCCGCC CCTGGCTCCG GAGGGCGACT 
     1561 CTGTGGCCAG CAAGCACGCC ATCTACGCCG TCCAGGTGAT CGCTGACCCT CCTGGGCCCG 
     1621 GGGAGGGGCC TCCTGCCCAG CACGCAGCCT TCCTGGTGCC CCTAGGCCCG GGACAGATCT 
     1681 CAGCAGAAGT GCAGGCCCCG CCCCCCGTGG TGAATGTGCG CGTCCATCAC CCGCCCGAGG 
     1741 CCTCAGTCCA GGTGCACCGC ATTGAGAGCT CGAACGCCGA GAGCGCAGCC CCCTCCCAGC 
     1801 ACCTGCTGCC GCACCCCAAG CCCTCGCACC CCCGGCCGCC CACCCAGAAG CCCCTGGGCC 
     1861 GCTGCTTTCA GGACACTCTG CCCAAGCAGC CGTGTGGCAG CAACCCCCTC CCCGGCCTCA 
     1921 CCAAGCAGGA AGACTGCTGC GGTAGCATCG GCACTGCCTG GGGCCAGAGC AAGTGCCACA 
     1981 AGTGTCCCCA GCTGCAGTAC ACAGGAGTGC AGAAGCCAGG GCCTGTACGT GGGGAAGTGG 
     2041 GCGCTGACTG TCCCCAGGGC TACAAGAGGC TTAACAGCAC CCACTGCCAG GACATCAACG 
     2101 AGTGCGCAAT GCCGGGCGTG TGTCGCCATG GTGACTGCCT CAACAACCCT GGCTCCTATC 
     2161 GCTGTGTCTG CCCACCTGGC CATAGTTTAG GCCCCTCCCG TACACAGTGC ATTGCAGACA 
     2221 AACCGGAGGA GAAGAGCCTG TGTTTCCGCC TGGTGAGCCC TGAGCACCAG TGCCAGCACC 
     2281 CACTGACCAC CCGCCTGACC CGCCAGCTCT GCTGCTGCAG TGTCGGCAAG GCCTGGGGCG 
     2341 CGCGGTGTCA GCGCTGCCCA ACAGATGGCA CCGCTGCGTT CAAGGAGATC TGCCCAGCTG 
     2401 GGAAGGGATA CCACATTCTC ACCTCCCACC AGACGCTCAC CATTCAGGGC GAGAGTGACT 
     2461 TTTCCCTTTT CCTGCACCCT GACGGGCCAC CCAAGCCCCA GCAGCTTCCG GAGAGCCCTA 
     2521 GCCAGGCTCC ACCACCTGAG GACACAGAGG AAGAGAGAGG GGTGACCACG GACTCACCGG 
     2581 TGAGTGAGGA GAGGTCAGTG CAGCAGAGCC ACCCAACTGC CACCACGACT CCTGCCCGGC 
     2641 CCTACCCCGA GCTGATCTCC CGTCCCTCGC CCCCGACCAT GCGCTGGTTC CTGCCGGACT 
     2701 TGCCTCCTTC CCGCAGCGCC GTAGAGATCG CTCCCACTCA GGTCACAGAG ACTGATGAGT 
     2761 GCCGACTGAA CCAGAACATC TGTGGCCACG GAGAGTGCGT GCCGGGCCCC CCTGACTACT 
     2821 CCTGCCACTG CAACCCCGGC TACCGGTCAC ATCCCCAGCA CCGCTACTGC GTGGATGTGA 
     2881 ACGAGTGCGA GGCAGAGCCC TGTGGCCCGG GGAGGGGCAT CTGCATGAAC ACCGGCGGCT 
     2941 CCTACAATTG CCACTGCAAC CGCGGCTACC GCCTGCACGT GGGCGCCGGG GGGCGCTCGT 
     3001 GCGTGGACCT GAACGAATGC GCCAAGCCCC ACCTGTGCGG CGACGGCGGC TTCTGCATCA 
     3061 ACTTTCCCGG TCACTACAAG TGCAACTGCT ACCCCGGCTA CCGGCTCAAA GCCTCCCGGC 
     3121 CTCCTGTGTG CGAAGACATC GACGAGTGCC GGGACCCAAG CTCTTGCCCG GATGGCAAAT 
     3181 GCGAGAACAA GCCCGGGAGC TTCAAGTGCA TCGCCTGTCA GCCTGGCTAC CGCAGCCAGG 
     3241 GGGGCGGGGC CTGTCGCGAC GTGAACGAGT GCGCCGAGGG CAGCCCCTGC TCGCCTGGCT 
     3301 GGTGCGAGAA CCTCCCGGGC TCCTTCCGCT GCACCTGTGC CCAGGGCTAC GCGCCCGCGC 
     3361 CCGACGGCCG CAGTTGCTTG GATGTGGACG AGTGTGAGGC TGGGGACGTG TGTGACAATG 
     3421 GCATCTGCAG CAACACGCCA GGATCTTTCC AGTGTCAGTG CCTCTCTGGC TACCATCTGT 
     3481 CCAGGGACCG GAGCCACTGC GAGGACATTG ATGAGTGTGA CTTCCCTGCA GCCTGCATTG 
     3541 GGGGTGACTG CATCAATACC AATGGCTCCT ACAGATGTCT TTGCCCCCAG GGGCATCGGC 
     3601 TGGTGGGTGG CAGGAAATGC CAAGACATAG ATGAGTGCAG CCAGGACCCG AGCCTGTGCC 
     3661 TTCCCCATGG GGCCTGCAAG AACCTTCAGG GCTCCTATGT GTGTGTCTGC GATGAGGGCT 
     3721 TCACTCCCAC CCAGGACCAG CACGGTTGTG AGGAGGTGGA GCAGCCCCAC CACAAGAAGG 
     3781 AGTGCTACCT GAACTTCGAT GACACAGTGT TCTGCGACAG CGTATTGGCC ACCAACGTGA 
     3841 CCCAGCAGGA GTGCTGCTGC TCTCTGGGGG CCGGCTGGGG CGACCACTGC GAAATCTACC 
     3901 CCTGCCCAGT CTACAGCTCA GCCGAGTTCC ACAGCCTCTG CCCAGACGGA AAGGGCTACA 
     3961 CCCAGGACAA CAACATCGTC AACTACGGCA TCCCAGCCCA CCGTGACATC GACGAGTGCA 
     4021 TGTTGTTCGG GTCGGAGATT TGCAAGGAGG GCAAGTGCGT GAACACGCAG CCTGGCTACG 
     4081 AGTGCTACTG CAAGCAGGGC TTCTACTACG ACGGGAACCT GCTGGAATGC GTGGACGTGG 
     4141 ACGAGTGCCT GGACGAGTCC AACTGCCGGA ACGGAGTGTG TGAGAACACG CGCGGCGGCT 
     4201 ACCGCTGTGC CTGCACGCCC CCTGCCGAGT ACAGTCCCGC GCAGCGCCAG TGCCTGAGCC 
     4261 CGGAAGAGAT GGACGTGGAC GAGTGCCAGG ACCCGGCAGC CTGCCGCCCT GGCCGCTGCG 
     4321 TCAACCTGCC GGGCTCCTAC CGCTGCGAGT GTCGCCCGCC CTGGGTGCCC GGGCCCTCCG 
     4381 GCCGCGATTG CCAGCTCCCC GAGAGCCCGG CCGAGCGTGC CCCGGAGCGG CGCGACGTGT 
     4441 GCTGGAGCCA GCGCGGAGAG GACGGCATGT GCGCTGGCCC CCTGGCCGGG CCTGCCCTCA 
     4501 CCTTCGACGA CTGCTGCTGC CGCCAGGGCC GCGGCTGGGG CGCCCAATGC CGACCGTGCC 
     4561 CGCCGCGCGG CGCGGGGTCC CATTGCCCGA CATCGCAGAG CGAGAGCAAT TCCTTCTGGG 
     4621 ACACAAGCCC CCTGCTGTTG GGGAAGCCCC CAAGAGATGA GGACAGTTCA GAGGAGGATT 
     4681 CAGACGAGTG TCGCTGCGTG AGTGGCCGCT GCGTGCCGCG GCCGGGCGGC GCCGTGTGCG 
     4741 AGTGTCCCGG CGGCTTCCAG CTCGACGCCT CCCGCGCCCG CTGCGTGGAT ATCGACGAGT 
     4801 GCCGAGAGCT GAACCAGCGC GGGCTGCTGT GCAAGAGCGA GCGCTGCGTG AACACCAGCG 
     4861 GCTCCTTCCG CTGCGTCTGC AAAGCCGGCT TCGCGCGCAG CCGCCCGCAC GGGGCCTGCG 
     4921 TTCCCCAGCG CCGCCGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4981 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5041 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5101 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5161 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5221 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5281 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5341 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5401 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5461 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5521 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5581 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5641 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5701 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5761 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5821 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5881 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5941 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6001 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6061 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6121 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6181 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6241 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6301 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6361 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6421 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6481 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6541 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6601 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6661 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6721 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6781 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6841 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6901 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6961 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7021 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7081 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7141 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7201 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7261 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7321 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7381 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7441 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7501 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7561 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7621 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7681 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7741 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7801 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7861 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7921 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7981 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8041 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8221 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8281 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8341 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8401 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8461 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8521 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8581 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8641 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8701 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8761 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//