LOCUS       RC226378                8796 bp    DNA     circular  SYN 
DEFINITION  UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1
ACCESSION   NM_001105562
VERSION     NM_001105562.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4951..5042 
                     /label="Myc-DDK"  
     misc_feature    5072..5657 
                     /label="PolyA signal"  
     misc_feature    5129..5148 
                     /label="Restriction Enzyme"  
     misc_feature    5933..6605 
                     /label="ColE1"  
     misc_feature    7035..7829 
                     /label="Kan/Neo"  
     misc_feature    7864..8166 
                     /label="SV40 ori"  
     misc_feature    8252..8729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4926..4933 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4926 
                     /label="UBE4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGCTG AGCGCTGATG AGATTCGACG GAGGCGCCTT GCACGACTTG 
     1081 CTGGTGGACA GACCTCTCAG CCAACCACCC CACTCACCTC TCCCCAGAGG GAGAACCCTC 
     1141 CGGGGCCTCC CATAGCGGCA TCAGCCCCAG GACCCTCTCA GAGTCTTGGT CTCAATGTCC 
     1201 ACAACATGAC CCCAGCTACC TCCCCAATAG GTGCATCAGG AGTAGCCCAT CGAAGCCAGA 
     1261 GCAGTGAAGG AGTCAGTTCT CTCAGCAGCT CGCCCTCTAA TAGCCTTGAA ACGCAATCTC 
     1321 AGTCTCTCTC ACGTTCCCAG AGCATGGATA TCGATGGTGT CTCATGTGAG AAAAGCATGT 
     1381 CCCAGGTGGA TGTGGATTCA GGAATTGAAA ACATGGAGGT TGATGAAAAT GATCGAAGAG 
     1441 AAAAGCGGAG CCTCAGTGAT AAGGAGCCTT CCTCGGGCCC TGAAGTGTCT GAAGAGCAGG 
     1501 CCTTACAGCT GGTCTGTAAG ATCTTCCGTG TCTCTTGGAA GGACCGGGAC AGAGATGTCA 
     1561 TCTTTCTTTC TTCTCTTTCT GCACAGTTTA AGCAGAACCC AAAAGAAGTA TTCTCCGATT 
     1621 TTAAGGACTT GATTGGCCAG ATTTTAATGG AAGTGCTAAT GATGTCCACT CAGACCAGAG 
     1681 ATGAAAACCC ATTTGCCAGT CTGACAGCCA CATCACAGCC AATTGCTGCA GCAGCACGGT 
     1741 CACCAGACAG AAATCTCTTG CTAAACACTG GCTCCAATCC AGGAACAAGC CCCATGTTCT 
     1801 GCAGCGTGGC TTCCTTTGGT GCCAGCTCTT TGTCTAGCCT CTATGAAAGT AGTCCGGCTC 
     1861 CCACTCCCAG TTTCTGGAGC TCTGTTCCCG TGATGGGCCC GTCTCTTGCC TCACCTTCCC 
     1921 GTGCAGCCAG CCAGTTGGCT GTGCCTTCCA CTCCCCTCAG TCCTCACAGT GCAGCCTCTG 
     1981 GAACTGCTGC GGGAAGCCAG CCTTCATCCC CGCGGTATCG CCCCTACACT GTCACTCACC 
     2041 CATGGGCGTC CTCAGGCGTC TCCATTCTGT CGAGCTCCCC AAGTCCCCCT GCCCTCGCCA 
     2101 GTAGCCCCCA AGCAGTGCCC GCCAGCAGTT CCAGACAGAG GCCCAGCAGC ACGGGTCCAC 
     2161 CCCTACCACC CGCCTCACCC AGTGCCACGA GCAGACGCCC CTCCTCCCTG AGGATCTCTC 
     2221 CTAGTTTGGG AGCCTCTGGT GGAGCAAGTA ATTGGGATTC CTACAGTGAC CATTTCACCA 
     2281 TTGAAACCTG CAAAGAGACA GATATGCTGA ACTACCTCAT CGAGTGTTTC GACCGAGTTG 
     2341 GAATAGAGGA AAAAAAAGCA CCAAAGATGT GCAGCCAGCC AGCAGTCAGC CAGCTTCTGA 
     2401 GCAACATCCG CTCACAGTGC ATATCCCATA CTGCTTTAGT ACTACAAGGC TCCCTAACAC 
     2461 AGCCCAGGTC CTTGCAGCAG CCGTCCTTCC TAGTGCCGTA TATGCTGTGT AGGAATCTCC 
     2521 CATATGGCTT CATTCAGGAA CTGGTGAGAA CCACTCACCA GGATGAAGAA GTGTTCAAGC 
     2581 AGATATTTAT CCCCATTTTA CAAGGCCTGG CTCTTGCTGC CAAAGAGTGC TCCCTCGACA 
     2641 GTGACTACTT TAAATACCCC CTCATGGCAC TAGGTGAGCT CTGTGAAACC AAGTTTGGGA 
     2701 AGACACACCC TGTGTGCAAT TTGGTTGCTT CTTTGCGGTT GTGGTTGCCG AAATCCTTAA 
     2761 GTCCTGGCTG TGGGCGGGAG CTGCAGAGAC TCTCTTACTT AGGGGCTTTC TTTAGCTTCT 
     2821 CAGTCTTTGC AGAAGATGAT GTTAAAGTGG TTGAAAAATA CTTCTCAGGG CCTGCCATTA 
     2881 CCCTGGAAAA CACTCGTGTG GTTAGCCAAT CATTGCAGCA TTACTTAGAG CTCGGAAGGC 
     2941 AAGAGCTTTT TAAGATTCTG CATAGTATTT TGTTAAATGG CGAAACCCGT GAGGCTGCTC 
     3001 TCAGTTACAT GGCGGCTGTC GTCAATGCCA ATATGAAGAA AGCACAGATG CAGACAGATG 
     3061 ATAGATTGGT GTCTACAGAT GGATTTATGC TGAATTTCCT TTGGGTACTG CAGCAGCTAA 
     3121 GTACAAAAAT CAAGTTAGAA ACAGTTGATC CCACGTATAT TTTTCACCCA AGATGTCGGA 
     3181 TTACTCTTCC CAATGATGAG ACGCGTGTGA ATGCAACGAT GGAAGATGTG AATGACTGGC 
     3241 TGACTGAACT CTATGGCGAT CAGCCTCCAT TTTCTGAGCC GAAATTCCCT ACGGAGTGCT 
     3301 TCTTTCTCAC CCTGCATGCT CACCACCTCT CTATTCTGCC TAGTTGCCGT CGCTATATCC 
     3361 GCAGACTCCG GGCTATCCGG GAGCTCAATA GAACTGTAGA AGATTTGAAA AATAATGAAA 
     3421 GCCAATGGAA AGATTCCCCA CTGGCAACTA GACACCGCGA AATGCTGAAG CGCTGTAAAA 
     3481 CTCAGCTTAA GAAACTGGTA CGGTGCAAGG CCTGTGCTGA TGCTGGCCTA CTTGACGAGA 
     3541 GCTTCCTGAG AAGATGTCTG AATTTTTATG GCCTTCTCAT TCAGCTGCTG CTCCGCATCC 
     3601 TGGACCCCGC ATATCCCGAT ATAACACTGC CTTTAAATTC AGATGTCCCC AAGGTATTTG 
     3661 CAGCGTTGCC TGAGTTTTAT GTAGAAGATG TTGCAGAATT TTTATTTTTT ATTGTACAAT 
     3721 ACTCTCCCCA GGCGCTTTAT GAGCCCTGTA CTCAGGATAT TGTGATGTTC CTTGTTGTGA 
     3781 TGTTGTGCAA CCAGAACTAC ATCCGAAACC CATATTTGGT GGCCAAACTG GTAGAAGTCA 
     3841 TGTTTATGAC CAACCCTGCT GTTCAGCCAC GAACCCAGAA GTTTTTTGAA ATGATTGAGA 
     3901 ACCATCCTCT CTCCACCAAG TTGTTGGTAC CTTCCCTGAT GAAGTTTTAT ACAGATGTTG 
     3961 AGCATACCGG AGCCACCAGT GAGTTTTATG ACAAGTTCAC AATTCGCTAT CATATTAGCA 
     4021 CCATTTTTAA AAGCCTTTGG CAAAACATAG CTCACCATGG CACCTTTATG GAGGAGTTCA 
     4081 ACTCCGGGAA GCAGTTTGTT CGCTATATAA ACATGTTGAT AAACGACACG ACGTTTTTGC 
     4141 TCGATGAAAG TCTGGAGTCT CTGAAGCGAA TCCATGAAGT GCAGGAAGAG ATGAAGAACA 
     4201 AAGAACAGTG GGACCAGTTG CCCCGGGATC AGCAGCAGGC TCGTCAGTCT CAGCTTGCTC 
     4261 AGGATGAGCG TGTGTCCCGC TCTTACCTCG CCCTGGCCAC CGAAACCGTG GACATGTTCC 
     4321 ACATCCTCAC GAAGCAGGTC CAGAAGCCCT TCCTCAGACC GGAGCTTGGA CCCCGATTGG 
     4381 CTGCAATGCT GAACTTTAAT CTTCAGCAAC TTTGTGGCCC CAAGTGCCGT GACCTGAAAG 
     4441 TTGAAAACCC TGAGAAATAC GGCTTTGAAC CAAAGAAGCT GTTGGACCAA CTGACGGATA 
     4501 TTTACTTACA GCTGGACTGT GCTCGGTTCG CGAAAGCCAT TGCTGACGAC CAGAGATCCT 
     4561 ACAGTAAGGA ATTGTTTGAA GAAGTTATTT CAAAGATGCG GAAGGCAGGG ATCAAATCCA 
     4621 CAATAGCAAT AGAAAAATTT AAGCTGCTCG CCGAGAAAGT GGAGGAGATA GTGGCCAAGA 
     4681 ACGCACGCGC AGAAATCGAC TACAGCGACG CTCCTGATGA GTTCAGAGAC CCTCTGATGG 
     4741 ACACCCTCAT GACAGACCCC GTGCGGCTGC CCTCTGGCAC CATCATGGAC CGCTCCATCA 
     4801 TCCTGCGGCA CCTGCTCAAC TCCCCCACGG ACCCCTTCAA CCGGCAGACG CTGACAGAGA 
     4861 GCATGCTGGA ACCAGTGCCA GAACTGAAAG AGCAGATTCA GGCGTGGATG AGAGAGAAAC 
     4921 AGAACAGCGA TCACAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//