LOCUS RC226376 8691 bp DNA circular SYN DEFINITION PRG4 (Myc-DDK-tagged)-Human proteoglycan 4 (PRG4), transcript variant D ACCESSION NM_001127710 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4848..4939 /label="Myc-DDK" misc_feature 4969..5554 /label="PolyA signal" misc_feature 5026..5045 /label="Restriction Enzyme" misc_feature 5830..6502 /label="ColE1" misc_feature 6932..7726 /label="Kan/Neo" misc_feature 7761..8063 /label="SV40 ori" misc_feature 8149..8626 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4830..4836 /label="Restriction Enzyme" misc_feature 1020..4830 /label="PRG4" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCTTGGAAG ACCCTGCCAA TTTATCTGCT CCTTCTGCTG AGCGTCTTTG 1081 TGATACAGCA GGTGAGTAGT CAGGAGCTCT CATGCAAGGG CAGGTGTTTC GAGTCTTTCG 1141 AGCGCGGACG GGAGTGCGAT TGCGACGCTC AGTGCAAAAA GTATGACAAG TGCTGCCCTG 1201 ACTACGAAAG CTTTTGCGCG GAGGTGAAGG ATAACAAAAA AAACAGAACA AAAAAGAAAC 1261 CCACCCCTAA ACCACCGGTT GTGGATGAGG CCGGCTCTGG GCTGGACAAT GGAGACTTTA 1321 AAGTCACCAC TCCAGACACG TCAACCACAC AACACAACAA AGTAAGCACC TCACCAAAGA 1381 TAACCACAGC CAAGCCCATT AATCCACGGC CATCCTTGCC TCCTAACAGC GACACATCAA 1441 AAGAAACTTC TTTGACCGTG AACAAGGAAA CCACGGTCGA GACCAAGGAA ACTACAACCA 1501 CTAATAAGCA GACCTCCACC GACGGGAAGG AGAAGACCAC TTCAGCTAAA GAGACACAGT 1561 CCATAGAGAA AACTTCTGCA AAAGATCTGG CACCAACGTC TAAGGTGCTC GCTAAACCGA 1621 CTCCGAAAGC AGAAACCACC ACAAAGGGTC CCGCTCTCAC TACTCCAAAG GAGCCAACAC 1681 CTACCACACC CAAAGAACCC GCTTCAACCA CACCTAAGGA GCCCACGCCC ACCACAATTA 1741 AGTCCGCCCC GACTACACCC AAGGAGCCTG CCCCGACCAC AACAAAATCT GCCCCTACTA 1801 CCCCGAAGGA GCCCGCTCCA ACAACCACTA AGGAGCCCGC ACCGACTACC CCTAAGGAAC 1861 CCGCCCCTAC GACGACAAAG GAACCAGCTC CAACCACGAC CAAGAGCGCT CCCACCACCC 1921 CTAAGGAGCC TGCCCCCACA ACTCCTAAGA AGCCGGCTCC TACAACCCCG AAGGAACCAG 1981 CCCCAACCAC GCCAAAAGAA CCCACTCCAA CAACCCCTAA AGAGCCAGCA CCTACAACTA 2041 AAGAGCCTGC TCCGACAACC CCTAAGGAGC CTGCTCCTAC AGCCCCCAAA AAACCAGCGC 2101 CCACCACGCC CAAGGAGCCC GCTCCTACAA CCCCCAAAGA ACCAGCCCCA ACCACTACTA 2161 AGGAACCGTC ACCCACTACC CCAAAAGAAC CGGCACCCAC TACCACAAAA TCAGCCCCCA 2221 CTACCACAAA AGAGCCAGCG CCAACCACTA CCAAAAGTGC GCCTACTACA CCGAAAGAGC 2281 CTAGCCCAAC CACTACAAAA GAACCGGCGC CCACAACTCC CAAAGAACCC GCTCCGACCA 2341 CCCCTAAGAA ACCAGCACCT ACTACACCCA AGGAACCCGC CCCTACCACA CCAAAAGAGC 2401 CAGCCCCCAC CACAACGAAA AAACCCGCGC CTACCACACC CAAAGAACCC GCCCCCACTA 2461 CTCCAAAGGA GACCGCTCCT ACCACGCCAA AGAAACTGAC CCCCACTACG CCGGAGAAAC 2521 TGGCACCAAC CACCCCAGAG AAACCCGCCC CCACTACTCC CGAGGAACTC GCTCCGACGA 2581 CACCCGAAGA ACCCACGCCT ACAACGCCTG AGGAGCCTGC CCCTACAACC CCAAAAGCCG 2641 CAGCGCCAAA TACCCCCAAG GAACCTGCGC CGACTACTCC TAAGGAGCCC GCACCCACAA 2701 CACCAAAGGA GCCAGCGCCG ACAACTCCTA AAGAGACCGC TCCAACTACG CCTAAAGGGA 2761 CTGCACCTAC GACATTGAAA GAACCTGCGC CTACGACCCC TAAGAAACCT GCTCCCAAGG 2821 AACTCGCTCC CACTACCACA AAAGAGCCAA CGTCCACCAC ATGCGATAAA CCAGCCCCGA 2881 CCACTCCCAA GGGTACGGCT CCAACCACAC CCAAGGAACC TGCCCCAACT ACACCGAAGG 2941 AGCCCGCCCC AACGACCCCA AAGGGGACTG CTCCTACTAC GTTGAAAGAA CCAGCCCCCA 3001 CAACACCAAA AAAACCCGCC CCCAAAGAGC TCGCCCCTAC AACTACAAAG GGCCCTACTA 3061 GTACCACCAG TGACAAACCC GCACCAACAA CCCCGAAAGA GACTGCCCCT ACCACACCTA 3121 AGGAGCCGGC GCCTACCACC CCCAAAAAAC CTGCCCCGAC AACCCCTGAA ACCCCTCCCC 3181 CAACAACAAG CGAGGTGTCA ACCCCCACGA CTACCAAGGA GCCCACTACC ATTCACAAAT 3241 CTCCTGATGA GAGCACCCCA GAGTTGAGTG CGGAGCCCAC TCCGAAAGCT CTGGAAAATA 3301 GTCCAAAAGA GCCAGGTGTC CCCACTACAA AGACTCCTGC TGCTACCAAG CCCGAAATGA 3361 CAACTACCGC CAAGGATAAA ACCACCGAGC GAGATCTGCG GACAACACCC GAGACTACTA 3421 CAGCCGCACC AAAAATGACG AAAGAAACAG CCACAACCAC CGAAAAAACC ACAGAGTCCA 3481 AGATAACCGC CACCACCACG CAAGTGACAT CTACCACAAC ACAGGATACT ACGCCCTTCA 3541 AAATCACCAC ACTTAAAACA ACTACACTGG CACCAAAGGT GACAACCACT AAGAAGACAA 3601 TAACAACGAC TGAGATAATG AATAAGCCCG AAGAGACAGC GAAACCAAAG GACAGAGCTA 3661 CCAATTCCAA AGCCACCACC CCAAAGCCAC AAAAACCAAC AAAGGCACCC AAAAAGCCTA 3721 CCAGTACCAA AAAACCAAAG ACCATGCCTA GAGTACGGAA GCCCAAGACA ACACCCACAC 3781 CGCGCAAAAT GACGAGCACT ATGCCAGAAC TTAATCCCAC ATCCCGGATC GCAGAAGCTA 3841 TGTTGCAGAC CACTACCCGC CCCAATCAGA CACCGAACAG TAAACTCGTC GAAGTCAACC 3901 CCAAATCAGA GGATGCCGGA GGCGCCGAAG GGGAGACTCC ACATATGCTC CTGCGACCGC 3961 ATGTTTTTAT GCCTGAAGTG ACCCCTGACA TGGATTACCT TCCTAGGGTC CCTAATCAGG 4021 GTATCATCAT CAATCCTATG CTCAGTGATG AGACCAATAT TTGTAACGGG AAACCAGTGG 4081 ATGGACTGAC AACACTGCGC AACGGCACAC TGGTTGCTTT TCGCGGTCAT TATTTTTGGA 4141 TGCTGTCCCC ATTCTCACCA CCATCCCCAG CTCGAAGGAT CACAGAGGTG TGGGGCATTC 4201 CTTCTCCAAT TGACACCGTT TTCACCCGCT GCAATTGCGA AGGAAAAACC TTCTTTTTCA 4261 AAGACAGTCA GTATTGGCGA TTTACAAATG ATATCAAAGA CGCAGGGTAT CCCAAGCCGA 4321 TCTTCAAAGG CTTCGGAGGC CTGACAGGCC AGATTGTTGC CGCTCTCTCC ACCGCTAAGT 4381 ACAAGAACTG GCCTGAATCC GTGTATTTCT TTAAGAGAGG GGGAAGCATT CAACAATATA 4441 TCTATAAGCA GGAGCCCGTG CAGAAATGCC CAGGCAGACG GCCCGCTCTG AACTACCCAG 4501 TTTACGGCGA GACTACCCAG GTAAGGAGGA GGCGCTTCGA GAGAGCTATT GGGCCCTCCC 4561 AAACCCACAC AATACGGATA CAGTACTCTC CGGCAAGACT GGCATACCAG GACAAGGGAG 4621 TGCTCCACAA CGAGGTAAAG GTTAGCATAT TGTGGCGGGG CTTGCCAAAC GTTGTAACAT 4681 CTGCCATAAG CCTGCCCAAC ATTCGCAAGC CTGACGGATA CGACTACTAT GCATTTAGCA 4741 AAGATCAGTA TTACAATATA GACGTGCCTT CCAGAACTGC CCGCGCAATT ACCACCAGGT 4801 CCGGCCAGAC ACTCTCCAAG GTGTGGTATA ATTGCCCTAC GCGTACGCGG CCGCTCGAGC 4861 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 4921 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 4981 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 5041 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 5101 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 5161 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 5221 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 5281 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 5341 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 5401 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 5461 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 5521 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 5581 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 5641 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 5701 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 5761 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 5821 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 5881 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 5941 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 6001 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 6061 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 6121 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 6181 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 6241 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 6301 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 6361 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 6421 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 6481 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 6541 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 6601 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 6661 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 6721 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 6781 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 6841 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 6901 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 6961 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 7021 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 7081 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 7141 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 7201 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 7261 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 7321 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 7381 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 7441 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 7501 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 7561 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 7621 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 7681 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 7741 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 7801 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 7861 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 7921 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 8041 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 8101 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 8161 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 8221 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 8281 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 8341 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 8401 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 8461 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 8521 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 8581 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 8641 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T //