LOCUS       RC226375                8658 bp    DNA     circular  SYN 
DEFINITION  SSFA2 (Myc-DDK-tagged)-Human sperm specific antigen 2 (SSFA2), transcript variant 1
ACCESSION   NM_001130445
VERSION     NM_001130445.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="SSFA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCGGCCC CTGTCGTCGT CGGCGGAGGC GGAGGAGGAA CTGGAGTGGC 
     1081 AAGTGGCGAG TCGCAGGAGG AAGGCCTGGG CCAAGTGCCG CAGCTCCTGG CAAGCGTCGG 
     1141 AGACGGAGGA TCTGTCCACA GAAGCGACGA CGCAGGACGA GGAGGAGGAC GAGGAGGAGG 
     1201 ACCTCCCCGG CGCGCAGCTG CCGGCAGCGG GGGGAAGAGG AAACGTGCCC AACGAGAAGA 
     1261 TCGCGATATG GCTCAAGGAC TGCCGTACAC CTTTGGGAGC CTCACTGGAT GAACAAAGCA 
     1321 GTAGTACACT CAAGGGTGTG CTTGTGAGAA ATGGAGGAAG TTTTGAAGAT GATTTGTCAT 
     1381 TGGGAGCTGA AGCCAACCAC CTCCATGAAA GTGATGCTCA AATTGAAAAC TGCAATAATA 
     1441 TCTTGGCCAA AGAGAGAAGA TTACAGTTTC ATCAGAAAGG GAGAAGTATG AATTCCACTG 
     1501 GATCTGGGAA AAGTAGTGGG ACAGTTTCAA GTGTTTCAGA ATTGTTGGAA CTTTATGAGG 
     1561 AAGATCCTGA AGAAATTCTT TATAATCTTG GATTTGGACG TGATGAACCA GATATTGCTT 
     1621 CTAAAATTCC TTCCAGATTT TTTAATTCAT CATCCTTTGC CAAAGGGATA GATATTAAAG 
     1681 TATTTTTGAG TGCTCAGATG CAACGGATGG AAGTAGAAAA CCCAAATTAT GCTTTAACAA 
     1741 GCCGTTTTCG TCAAATTGAA GTGCTTACTA CTGTGGCCAA TGCGTTTTCT TCTTTATATT 
     1801 CTCAAGTCTC CGGGACGCCC CTGCAGAGAA TTGGAAGTAT GTCCTCAGTG ACCTCTAACA 
     1861 AGGAGACAGA CCCACCTCCA CCTTTAACTC GAAGTAACAC TGCAAATCGT TTAATGAAAA 
     1921 CACTCTCAAA ACTGAATTTA TGTGTTGATA AAACAGAGAA AGGAGAAAGT AGTAGTCCTT 
     1981 CTCCATCAGC TGAAAAAGGA AAGATTCTAA ATGTTTCAGT GATTGAAGAA AGTGGCAATA 
     2041 AAAACGATCA AAAGTCTCAA AAAATTATGA AGAAGAAAGA GTCATCTTCT ATGTTGGCTA 
     2101 CAGTTAAAGA AGAAGTCTCT GGTAGTTCAG CAGCTGTTAC GGAGAATGCT GATAGTGATA 
     2161 GAATTTCTGA TGAAGCAAAT AGTAATTTTA ACCAAGGAAC TGAAAATGAA CAAAGTAAAG 
     2221 AAACTCAAAG TCATGAGAGT AAACTGGGTG AGGAATCTGG TATTGTAGAA TCCAAATTAG 
     2281 ATAGTGATTT CAACATATCC AGCCACAGTG AGCTGGAAAA TAGCAGTGAG CTGAAAAGTG 
     2341 TCCATATATC CACACCTGAA AAAGAGCCTT GTGCACCACT GACAATACCA TCCATAAGAA 
     2401 ATATAATGAC ACAGCAGAAG GACTCCTTCG AAATGGAAGA GGTTCAAAGT ACGGAGGGAG 
     2461 AAGCTCCTCA TGTTCCAGCC ACTTACCAGC TAGGTCTTAC GAAGTCGAAA AGAGATCATC 
     2521 TGTTACGTAC TGCAAGTCAG CATTCCGATA GCAGTGGTTT TGCTGAAGAT TCTACAGACT 
     2581 GCCTATCCCT TAATCATCTT CAGGTTCAGG AGTCCTTGCA GGCTATGGGG AGTAGTGCTG 
     2641 ATAGTTGTGA CAGTGAGACA ACAGTTACGT CACTTGGTGA AGACCTTGCC ACACCAACAG 
     2701 CACAAGACCA GCCTTATTTT AATGAATCAG AGGAGGAGTC TCTTGTCCCT CTTCAGAAGG 
     2761 GACTAGAGAA GGCAGCAGCA GTTGCAGACA AAAGAAAATC AGGTAGCCAG GATTTCCCTC 
     2821 AGTGCAACAC CATTGAGAAT ACAGGAACTA AACAGTCCAC CTGTAGTCCA GGGGATCATA 
     2881 TCATTGAAAT TACTGAAGTG GAAGAGGATT TGTTTCCAGC AGAGACAGTA GAGCTACTGA 
     2941 GGGAAGCAAG TGCTGAAAGT GATGTGGGTA AAAGCAGTGA AAGTGAATTT ACTCAGTATA 
     3001 CCACACACCA TATTCTGAAA TCATTGGCTT CTATTGAAGC TAAATGCAGT GATATGAGCT 
     3061 CTGAAAATAC AACTGGGCCT CCCTCTTCCA TGGACAGAGT TAATACAGCT TTGCAAAGAG 
     3121 CTCAAATGAA GGTTTGCAGT CTGTCTAATC AAAGGATGGG GCGTAGCCTG CTAAAATCAA 
     3181 AAGATTTGTT AAAACAAAGG TACTTATTTG CAAAAGCTGG CTATCCTCTA AGAAGGTCTC 
     3241 AGTCTTTACC AACCACCTTA TTGAGCCCAG TAAGGGTTGT GTCCTCTGTC AATGTTCGAT 
     3301 TATCTCCAGG AAAAGAGACC AGATGCAGCC CACCTTCCTT CACCTATAAG TACACACCTG 
     3361 AAGAGGAGCA GGAATTGGAA AAGCGGGTGA TGGAACATGA TGGTCAGTCT TTAGTTAAAT 
     3421 CGACCATTTT CATCTCTCCA TCATCTGTGA AGAAAGAAGA AGCCCCCCAG AGTGAGGCGC 
     3481 CGCGGGTGGA GGAATGCCAT CATGGAAGGA CTCCTACCTG TTCACGGCTT GCTCCACCAC 
     3541 CAATGTCTCA GTCTACCTGT TCCCTTCATT CCATCCACTC TGAGTGGCAA GAAAGGCCCC 
     3601 TGTGTGAGCA CACAAGAACT CTGAGCACTC ACAGTGTTCC CAACATATCA GGGGCTACTT 
     3661 GTAGTGCCTT CGCTTCCCCT TTCGGGTGTC CTTACTCACA TAGACATGCC ACCTACCCTT 
     3721 ACCGAGTGTG CTCTGTGAAT CCTCCTTCAG CCATAGAAAT GCAGTTGCGA AGAGTATTAC 
     3781 ATGATATTAG AAACTCACTG CAGAATCTTT CACAGTACCC TATGATGAGA GGACCTGATC 
     3841 CTGCTGCTGC TCCATATAGT ACTCAGAAAT CATCTGTTCT ACCTCTTTAT GAAAATACTT 
     3901 TTCAGGAGCT CCAGGTAATG AGGCGGAGCC TGAATTTGTT TAGAACACAA ATGATGGATT 
     3961 TAGAATTGGC AATGCTGCGT CAGCAAACCA TGGTTTATCA TCATATGACT GAGGAGGAGA 
     4021 GGTTTGAAGT TGATCAGCTC CAGGGTTTGA GAAATTCAGT CCGAATGGAA CTTCAGGACC 
     4081 TGGAACTGCA GCTGGAGGAG CGCCTGCTGG GCCTGGAGGA GCAGCTTCGT GCTGTGCGCA 
     4141 TGCCTTCACC CTTCCGCTCC TCCGCACTCA TGGGAATGTG TGGCAGTAGA AGCGCTGATA 
     4201 ACTTGTCATG CCCTTCTCCA TTGAATGTAA TGGAACCAGT CACTGAACTG ATGCAGGAGC 
     4261 AGTCATACCT GAAGTCTGAA TTGGGCCTGG GACTTGGAGA AATGGGATTT GAAATTCCTC 
     4321 CTGGAGAAAG CTCAGAATCT GTTTTTTCCC AAGCAACATC AGAATCATCT TCTGTATGTT 
     4381 CTGGTCCCTC TCATGCTAAC AGAAGAACTG GAGTACCTTC TACTGCCTCA GTGGGCAAAT 
     4441 CCAAAACCCC ATTAGTGGCA AGGAAGAAAG TGTTCCGAGC ATCGGTGGCT CTAACGCCAA 
     4501 CAGCTCCTTC TAGAACAGGC TCTGTGCAGA CACCTCCAGA TTTGGAAAGT TCTGAGGAAG 
     4561 TTGATGCAGC TGAAGGAGCC CCAGAAGTTG TAGGACCTAA ATCTGAAGTG GAAGAAGGGC 
     4621 ATGGAAAACT CCCATCAATG CCAGCTGCTG AGGAAATGCA TAAAAATGTG GAGCAAGATG 
     4681 AGTTGCAGCA AGTCATACGG GAGATTAAAG AGTCTATTGT TGGGGAAATC AGACGGGAAA 
     4741 TTGTAAGTGG ACTTTTGGCA GCAGTATCTT CAAGTAAAGC GTCTAATTCT AAGCAAGATT 
     4801 ATCATACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//