LOCUS       RC226372                8640 bp    DNA     circular  SYN 
DEFINITION  DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 3
ACCESSION   NM_001135040
VERSION     NM_001135040.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="DCTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAGAGC AAGAGGCACG TGTACAGCCG GACGCCCAGC GGCAGCAGGA 
     1081 TGAGTGCGGA GGCAAGCGCC CGGCCTCTGC GGGTGGGCTC CCGTGTAGAG GTGATTGGAA 
     1141 AAGGCCACCG AGGCACTGTG GCCTATGTTG GAGCCACACT GTTTGCCACT GGCAAATGGG 
     1201 TAGGCGTGAT TCTGGATGAA GCAAAGGGCA AAAATGATGG AACTGTTCAA GGCAGGAAGT 
     1261 ACTTCACTTG TGATGAAGGG CATGGCATCT TTGTGCGCCA GTCCCAGATC CAGGTATTTG 
     1321 AAGATGGAGC AGATACTACT TCCCCAGAGA CACCTGATTC TTCTGCTTCA AAAGTCCTCA 
     1381 AAAGAGAGGG AACTGATACA ACTGCAAAGA CTAGCAAACT GCCCACGCGC CCAGCCAGTA 
     1441 CTGGGGTGGC TGGGGCCAGT AGCTCCCTGG GCCCCTCTGG CTCAGCGTCA GCAGGTGAGC 
     1501 TGAGCAGCAG TGAGCCCAGC ACCCCGGCTC AGACTCCGCT GGCAGCACCC ATCATCCCCA 
     1561 CGCCGGTCCT CACCTCTCCT GGAGCAGTCC CCCCGCTTCC TTCCCCATCC AAGGAGGAGG 
     1621 AGGGACTAAG GGCTCAGGTG CGGGACCTGG AGGAGAAACT AGAGACCCTG AGACTGAAAC 
     1681 GGGCAGAAGA CAAAGCAAAG CTAAAAGAGC TGGAGAAACA CAAAATCCAG CTGGAGCAGG 
     1741 TGCAGGAATG GAAGAGCAAA ATGCAGGAGC AGCAGGCCGA CCTGCAGCGG CGCCTCAAGG 
     1801 AGGCGAGAAA GGAAGCCAAG GAGGCGCTGG AGGCAAAGGA ACGCTATATG GAGGAGATGG 
     1861 CTGATACTGC TGATGCCATT GAGATGGCCA CTTTGGACAA GGAGATGGCT GAAGAGCGGG 
     1921 CTGAGTCCCT GCAGCAGGAG GTGGAGGCAC TGAAGGAGCG GGTGGACGAG CTCACTACTG 
     1981 ACTTAGAGAT CCTCAAGGCT GAGATTGAAG AGAAGGGCTC AGATGGCGCT GCATCCAGTT 
     2041 ATCAGCTCAA GCAGCTTGAG GAGCAGAATG CCCGCCTGAA GGATGCCCTG GTGAGGATGC 
     2101 GGGATCTTTC TTCCTCAGAG AAGCAGGAGC ATGTGAAGCT CCAGAAGCTC ATGGAAAAGA 
     2161 AGAACCAAGA GCTGGAAGTT GTGAGGCAAC AGCGGGAGCG TCTGCAGGAG GAGCTAAGCC 
     2221 AGGCAGAGAG CACCATTGAT GAGCTCAAGG AGCAGGTGGA TGCTGCTCTG GGTGCTGAGG 
     2281 AGATGGTGGA GATGCTGACA GATCGGAACC TGAATCTGGA AGAGAAAGTG CGCGAGTTGA 
     2341 GGGAGACTGT GGGAGACTTG GAAGCGATGA ATGAGATGAA CGATGAGCTG CAGGAGAATG 
     2401 CACGTGAGAC AGAACTGGAG CTGCGGGAGC AGCTGGACAT GGCAGGCGCG CGGGTTCGTG 
     2461 AGGCCCAGAA GCGTGTGGAG GCAGCCCAGG AGACGGTTGC AGACTACCAG CAGACCATCA 
     2521 AGAAGTACCG CCAGCTGACC GCCCATCTAC AGGATGTGAA TCGGGAACTG ACAAACCAGC 
     2581 AGGAAGCATC TGTGGAGAGG CAACAGCAGC CACCTCCAGA GACCTTTGAC TTCAAAATCA 
     2641 AGTTTGCTGA GACTAAGGCC CATGCCAAGG CAATTGAGAT GGAATTGAGG CAGATGGAGG 
     2701 TGGCCCAGGC CAATCGACAC ATGTCCCTGC TGACAGCCTT CATGCCTGAC AGCTTCCTTC 
     2761 GGCCAGGTGG GGACCATGAC TGCGTTCTGG TGCTGTTGCT CATGCCTCGT CTCATTTGCA 
     2821 AGGCAGAGCT GATCCGGAAG CAGGCCCAGG AGAAGTTTGA ACTAAGTGAG AACTGTTCAG 
     2881 AGCGGCCTGG GCTGCGAGGA GCTGCTGGGG AGCAACTCAG CTTTGCTGCT GGACTGGTGT 
     2941 ACTCGCTGAG CCTGCTGCAG GCCACGCTAC ACCGCTATGA GCATGCCCTC TCTCAGTGCA 
     3001 GTGTGGATGT GTATAAGAAA GTGGGCAGCC TGTACCCTGA GATGAGTGCC CATGAGCGCT 
     3061 CCTTGGATTT CCTCATTGAA CTGCTGCACA AGGATCAGCT GGATGAGACT GTCAATGTGG 
     3121 AGCCTCTCAC CAAGGCCATC AAGTACTATC AGCATCTGTA CAGCATCCAC CTTGCCGAAC 
     3181 AGCCTGAGGA CTGTACTATG CAGCTGGCTG ACCACATTAA GTTCACGCAG AGTGCTCTGG 
     3241 ACTGCATGAG TGTGGAGGTA GGACGGCTGC GTGCCTTCTT GCAGGGTGGG CAGGAGGCTA 
     3301 CAGATATTGC CCTCCTGCTC CGGGATCTGG AAACTTCATG CAGTGACATC CGCCAGTTCT 
     3361 GCAAGAAGAT CCGAAGGCGA ATGCCAGGGA CAGATGCTCC TGGGATCCCA GCTGCACTGG 
     3421 CCTTTGGACC ACAGGTATCT GACACGCTCC TAGACTGCAG GAAACACTTG ACGTGGGTCG 
     3481 TGGCTGTGCT GCAGGAGGTG GCAGCTGCTG CTGCCCAGCT CATTGCCCCA CTGGCAGAGA 
     3541 ATGAGGGGCT ACTTGTGGCT GCTCTGGAGG AACTGGCTTT CAAAGCAAGC GAGCAGATCT 
     3601 ATGGGACCCC CTCCAGCAGC CCCTATGAGT GTCTGCGCCA GTCATGCAAC ATCCTCATCA 
     3661 GTACCATGAA CAAGCTGGCC ACAGCCATGC AGGAGGGGGA GTATGATGCA GAGCGGCCCC 
     3721 CCAGCAAGCC TCCACCGGTT GAACTGCGGG CTGCTGCCCT TCGTGCAGAG ATCACAGATG 
     3781 CTGAAGGCCT GGGTTTGAAG CTCGAAGATC GAGAGACAGT TATTAAGGAG TTGAAGAAGT 
     3841 CACTCAAGAT TAAGGGAGAG GAGCTAAGTG AGGCCAATGT GCGGCTGAGC CTCCTGGAGA 
     3901 AGAAGTTGGA CAGTGCTGCC AAGGATGCAG ATGAGCGCAT CGAGAAAGTC CAGACTCGGC 
     3961 TGGAGGAGAC CCAGGCACTG CTGCGAAAGA AGGAGAAAGA GTTTGAGGAG ACAATGGATG 
     4021 CACTCCAGGC TGACATCGAC CAGCTGGAGG CAGAGAAGGC AGAACTAAAG CAGCGTCTGA 
     4081 ACAGCCAGTC CAAACGCACG ATTGAGGGAC TCCGGGGCCC TCCTCCTTCA GGCATTGCTA 
     4141 CTCTGGTCTC TGGCATTGCT GGTGGAGCCA TCCCTGGGCA GGCTCCAGGG TCTGTGCCAG 
     4201 GCCCAGGGCT GGTGAAGGAC TCACCACTGC TGCTTCAGCA GATCTCTGCC ATGAGGCTGC 
     4261 ACATCTCCCA GCTCCAGCAT GAGAACAGCA TCCTCAAGGG AGCCCAGATG AAGGCATCCT 
     4321 TGGCATCCCT GCCCCCTCTG CATGTTGCAA AGCTATCCCA TGAGGGCCCT GGCAGTGAGT 
     4381 TACCAGCTGG AGCGCTGTAT CGTAAGACCA GCCAGCTGCT GGAGACATTG AATCAATTGA 
     4441 GCACACACAC GCACGTAGTA GACATCACTC GCACCAGCCC TGCTGCCAAG AGCCCGTCGG 
     4501 CCCAACTTAT GGAGCAAGTG GCTCAGCTTA AGTCCCTGAG TGACACCGTC GAGAAGCTCA 
     4561 AGGATGAGGT CCTCAAGGAG ACAGTATCTC AGCGCCCTGG AGCCACAGTA CCCACTGACT 
     4621 TTGCCACCTT CCCTTCATCA GCCTTCCTCA GGGCCAAGGA GGAGCAGCAG GATGACACAG 
     4681 TCTACATGGG CAAAGTGACC TTCTCATGTG CGGCTGGTTT TGGACAGCGA CACCGGCTGG 
     4741 TGCTGACCCA GGAGCAGCTG CACCAGCTTC ACAGTCGCCT CATCTCCACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//