LOCUS       RC226368                8598 bp    DNA     circular  SYN 
DEFINITION  POLG (Myc-DDK-tagged)-Human polymerase (DNA directed), gamma (POLG), transcript variant 2
ACCESSION   NM_001126131
VERSION     NM_001126131.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4755..4846 
                     /label="Myc-DDK"  
     misc_feature    4876..5461 
                     /label="PolyA signal"  
     misc_feature    4933..4952 
                     /label="Restriction Enzyme"  
     misc_feature    5737..6409 
                     /label="ColE1"  
     misc_feature    6839..7633 
                     /label="Kan/Neo"  
     misc_feature    7668..7970 
                     /label="SV40 ori"  
     misc_feature    8056..8533 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4737..4743 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4737 
                     /label="POLG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGCCTG CTCTGGAGGA AGGTGGCCGG CGCCACCGTC GGGCCAGGGC 
     1081 CGGTTCCAGC TCCGGGGCGC TGGGTCTCCA GCTCCGTCCC CGCGTCCGAC CCCAGCGACG 
     1141 GGCAGCGGCG GCGGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAACAG CAGCCTCAGC 
     1201 AGCCGCAAGT GCTATCCTCG GAGGGCGGGC AGCTGCGGCA CAACCCATTG GACATCCAGA 
     1261 TGCTCTCGAG AGGGCTGCAC GAGCAAATCT TCGGGCAAGG AGGGGAGATG CCTGGCGAGG 
     1321 CCGCGGTGCG CCGCAGCGTC GAGCACCTGC AGAAGCACGG GCTCTGGGGG CAGCCAGCCG 
     1381 TGCCCTTGCC CGACGTGGAG CTGCGCCTGC CGCCCCTCTA CGGGGACAAC CTGGACCAGC 
     1441 ACTTCCGCCT CCTGGCCCAG AAGCAGAGCC TGCCCTACCT GGAGGCGGCC AACTTGCTGT 
     1501 TGCAGGCCCA GCTGCCCCCG AAGCCCCCGG CTTGGGCCTG GGCGGAGGGC TGGACCCGGT 
     1561 ACGGCCCCGA GGGGGAGGCC GTACCCGTGG CCATCCCCGA GGAGCGGGCC CTGGTGTTCG 
     1621 ACGTGGAGGT CTGCTTGGCA GAGGGAACTT GCCCCACATT GGCGGTGGCC ATATCCCCCT 
     1681 CGGCCTGGTA TTCCTGGTGC AGCCAGCGGC TGGTGGAAGA GCGTTACTCT TGGACCAGCC 
     1741 AGCTGTCGCC GGCTGACCTC ATCCCCCTGG AGGTCCCTAC TGGTGCCAGC AGCCCCACCC 
     1801 AGAGAGACTG GCAGGAGCAG TTAGTGGTGG GGCACAATGT TTCCTTTGAC CGAGCTCATA 
     1861 TCAGGGAGCA GTACCTGATC CAGGGTTCCC GCATGCGTTT CCTGGACACC ATGAGCATGC 
     1921 ACATGGCCAT CTCAGGGCTA AGCAGCTTCC AGCGCAGTCT GTGGATAGCA GCCAAGCAGG 
     1981 GCAAACACAA GGTCCAGCCC CCCACAAAGC AAGGCCAGAA GTCCCAGAGG AAAGCCAGAA 
     2041 GAGGCCCAGC GATCTCATCC TGGGACTGGC TGGACATCAG CAGTGTCAAC AGTCTGGCAG 
     2101 AGGTGCACAG ACTTTATGTA GGGGGGCCTC CCTTAGAGAA GGAGCCTCGA GAACTGTTTG 
     2161 TGAAGGGCAC CATGAAGGAC ATTCGTGAGA ACTTCCAGGA CCTGATGCAG TACTGTGCCC 
     2221 AGGACGTGTG GGCCACCCAT GAGGTTTTCC AGCAGCAGCT ACCGCTCTTC TTGGAGAGGT 
     2281 GTCCCCACCC AGTGACTCTG GCCGGCATGC TGGAGATGGG TGTCTCCTAC CTGCCTGTCA 
     2341 ACCAGAACTG GGAGCGTTAC CTGGCAGAGG CACAGGGCAC TTATGAGGAG CTCCAGCGGG 
     2401 AGATGAAGAA GTCGTTGATG GATCTGGCCA ATGATGCCTG CCAGCTGCTC TCAGGAGAGA 
     2461 GGTACAAAGA AGACCCCTGG CTCTGGGACC TGGAGTGGGA CCTGCAAGAA TTTAAGCAGA 
     2521 AGAAAGCTAA GAAGGTGAAG AAGGAACCAG CCACAGCCAG CAAGTTGCCC ATCGAGGGGG 
     2581 CTGGGGCCCC TGGTGATCCC ATGGATCAGG AAGACCTCGG CCCCTGCAGT GAGGAGGAGG 
     2641 AGTTTCAACA AGATGTCATG GCCCGCGCCT GCTTGCAGAA GCTGAAGGGG ACCACAGAGC 
     2701 TCCTGCCCAA GCGGCCCCAG CACCTTCCTG GACACCCTGG ATGGTACCGG AAGCTCTGCC 
     2761 CCCGGCTAGA CGACCCTGCA TGGACCCCGG GCCCCAGCCT CCTCAGCCTG CAGATGCGGG 
     2821 TCACACCTAA ACTCATGGCA CTTACCTGGG ATGGCTTCCC TCTGCACTAC TCAGAGCGTC 
     2881 ATGGCTGGGG CTACTTGGTG CCTGGGCGGC GGGACAACCT GGCCAAGCTG CCGACAGGTA 
     2941 CCACCCTGGA GTCAGCTGGG GTGGTCTGCC CCTACAGAGC CATCGAGTCC CTGTACAGGA 
     3001 AGCACTGTCT CGAACAGGGG AAGCAGCAGC TGATGCCCCA GGAGGCCGGC CTGGCGGAGG 
     3061 AGTTCCTGCT CACTGACAAT AGTGCCATAT GGCAAACGGT AGAAGAACTG GATTACTTAG 
     3121 AAGTGGAGGC TGAGGCCAAG ATGGAGAACT TGCGAGCTGC AGTGCCAGGT CAACCCCTAG 
     3181 CTCTGACTGC CCGTGGTGGC CCCAAGGACA CCCAGCCCAG CTATCACCAT GGCAATGGAC 
     3241 CTTACAACGA CGTGGACATC CCTGGCTGCT GGTTTTTCAA GCTGCCTCAC AAGGATGGTA 
     3301 ATAGCTGTAA TGTGGGAAGC CCCTTTGCCA AGGACTTCCT GCCCAAGATG GAGGATGGCA 
     3361 CCCTGCAGGC TGGCCCAGGA GGTGCCAGTG GGCCCCGTGC TCTGGAAATC AACAAAATGA 
     3421 TTTCTTTCTG GAGGAACGCC CATAAACGTA TCAGCTCCCA GATGGTGGTG TGGCTGCCCA 
     3481 GGTCAGCTCT GCCCCGTGCT GTGATCAGGC ACCCCGACTA TGATGAGGAA GGCCTCTATG 
     3541 GGGCCATCCT GCCCCAAGTG GTGACTGCCG GCACCATCAC TCGCCGGGCT GTGGAGCCCA 
     3601 CATGGCTCAC CGCCAGCAAT GCCCGGCCTG ACCGAGTAGG CAGTGAGTTG AAAGCCATGG 
     3661 TGCAGGCCCC ACCTGGCTAC ACCCTTGTGG GTGCTGATGT GGACTCCCAA GAGCTGTGGA 
     3721 TTGCAGCTGT GCTTGGAGAC GCCCACTTTG CCGGCATGCA TGGCTGCACA GCCTTTGGGT 
     3781 GGATGACACT GCAGGGCAGG AAGAGCAGGG GCACTGATCT ACACAGTAAG ACAGCCACTA 
     3841 CTGTGGGCAT CAGCCGTGAG CATGCCAAAA TCTTCAACTA CGGCCGCATC TATGGTGCTG 
     3901 GGCAGCCCTT TGCTGAGCGC TTACTAATGC AGTTTAACCA CCGGCTCACA CAGCAGGAGG 
     3961 CAGCTGAGAA GGCCCAGCAG ATGTACGCTG CCACCAAGGG CCTCCGCTGG TATCGGCTGT 
     4021 CGGATGAGGG CGAGTGGCTG GTGAGGGAGT TGAACCTCCC AGTGGACAGG ACTGAGGGTG 
     4081 GCTGGATTTC CCTGCAGGAT CTGCGCAAGG TCCAGAGAGA AACTGCAAGG AAGTCACAGT 
     4141 GGAAGAAGTG GGAGGTGGTT GCTGAACGGG CATGGAAGGG GGGCACAGAG TCAGAAATGT 
     4201 TCAATAAGCT TGAGAGCATT GCTACGTCTG ACATACCACG TACCCCGGTG CTGGGCTGCT 
     4261 GCATCAGCCG AGCCCTGGAG CCCTCGGCTG TCCAGGAAGA GTTTATGACC AGCCGTGTGA 
     4321 ATTGGGTGGT ACAGAGCTCT GCTGTTGACT ACTTACACCT CATGCTTGTG GCCATGAAGT 
     4381 GGCTGTTTGA AGAGTTTGCC ATAGATGGGC GCTTCTGCAT CAGCATCCAT GACGAGGTTC 
     4441 GCTACCTGGT GCGGGAGGAG GACCGCTACC GCGCTGCCCT GGCCTTGCAG ATCACCAACC 
     4501 TCTTGACCAG GTGCATGTTT GCCTACAAGC TGGGTCTGAA TGACTTGCCC CAGTCAGTCG 
     4561 CCTTTTTCAG TGCAGTCGAT ATTGACCGGT GCCTCAGGAA GGAAGTGACC ATGGATTGTA 
     4621 AAACCCCTTC CAACCCAACT GGGATGGAAA GGAGATACGG GATTCCCCAG GGTGAAGCGC 
     4681 TGGATATTTA CCAGATAATT GAACTCACCA AAGGCTCCTT GGAAAAACGA AGCCAGCCTG 
     4741 GACCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4801 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4861 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4921 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4981 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5041 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5101 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5161 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5221 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5281 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5341 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5401 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5461 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5521 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5581 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5641 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5701 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5761 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5821 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5881 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5941 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6001 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6061 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6121 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6181 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6241 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6301 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6361 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6421 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6481 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6541 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6601 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6661 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6721 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6781 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6841 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6901 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6961 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7021 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7081 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7141 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7201 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7261 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7321 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7381 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7441 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7501 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7561 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7621 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7681 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7741 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7801 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8041 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8101 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8161 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8221 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8281 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8341 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8401 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8461 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8521 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8581 AAATTTAACG CGAATTTT
//