LOCUS       RC226365                8586 bp    DNA     circular  SYN 
DEFINITION  AUTS2 (Myc-DDK-tagged)-Human autism susceptibility candidate 2 (AUTS2), transcript variant 2
ACCESSION   NM_001127231
VERSION     NM_001127231.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="AUTS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGGCCCG ACGCGGGGCC ATGGACTCCG CAAAAAGCGG CGGTCGCGGT 
     1081 CGCAGCGAGA CCGGGAGAGG CGCTCCCGGG GCGGGCTGGG GGCCGGCGCG GCCGGCGGCG 
     1141 GCGGGGCTGG CCGGACCCGG GCGCTCTCAC TCGCCTCGTC GTCGGGCTCC GACAAGGAAG 
     1201 ACAATGGGAA GCCCCCGTCC TCCGCCCCGT CCCGGCCCAG ACCCCCGCGG AGGAAGCGGA 
     1261 GAGAGTCCAC CTCGGCAGAA GAGGACATCA TTGATGGATT TGCCATGACC AGCTTTGTCA 
     1321 CTTTTGAAGC GCTGGAGAAA GATGTAGCAC TTAAGCCTCA GGAACGTGTG GAGAAACGCC 
     1381 AGACGCCCCT GACCAAGAAG AAACGAGAAG CACTTACCAA TGGCTTGTCC TTTCATTCAA 
     1441 AGAAGAGCAG ACTCAGCCAC CCACACCACT ACAGCTCAGA TCGAGAAAAT GACCGCAATC 
     1501 TCTGCCAGCA CCTTGGGAAG AGAAAGAAAA TGCCGAAGGC ACTCAGACAG CTCAAGCCAG 
     1561 GACAGAACAG CTGCAGGGAC AGTGACAGTG AAAGTGCCAG TGGAGAATCC AAGGGCTTCC 
     1621 ACCGGAGCAG CTCTCGGGAA AGGCTCAGTG ATAGTTCAGC TCCTTCCAGC TTGGGAACAG 
     1681 GCTACTTCTG TGACAGTGAC AGTGACCAGG AAGAGAAGGC ATCAGATGCC AGCTCTGAAA 
     1741 AACTCTTCAA CACTGTTATT GTAAACAAAG ATCCGGAGTT AGGTGTTGGC ACGCTACCAG 
     1801 AACATGACAG CCAGGATGCA GGGCCGATTG TCCCCAAGAT ATCGGGTCTA GAGAGAAGCC 
     1861 AGGAGAAGAG CCAGGACTGT TGCAAAGAGC CAATCTTTGA GCCTGTGGTG CTTAAAGACC 
     1921 CCTGCCCTCA GGTCGCACAG CCAATACCCC AGCCGCAGAC GGAGCCCCAA CTCCGAGCTC 
     1981 CTTCTCCGGA CCCTGACTTG GTGCAGCGCA CAGAGGCCCC ACCTCAACCC CCACCTCTGA 
     2041 GTACACAGCC ACCACAGGGC CCTCCTGAGG CCCAGCTCCA GCCTGCCCCG CAGCCTCAGG 
     2101 TGCAGAGGCC ACCCAGGCCA CAGTCCCCCA CCCAGCTGCT CCATCAGAAC CTCCCACCTG 
     2161 TGCAGGCCCA CCCCTCTGCT CAGAGCCTCT CCCAGCCATT GTCAGCCTAC AACAGCAGTA 
     2221 GCTTAAGCCT CAACAGTTTA AGCAGCAGCA GAAGCAGCAC TCCAGCGAAG ACTCAGCCCG 
     2281 CCCCACCTCA CATCTCCCAC CACCCCTCTG CCTCCCCGTT CCCCCTCTCC CTGCCCAACC 
     2341 ACAGCCCCCT GCACAGCTTC ACACCCACCC TCCAGCCCCC CGCACACTCA CATCACCCCA 
     2401 ATATGTTTGC CCCTCCCACT GCTCTGCCTC CTCCACCACC ACTGACATCA GGAAGTCTGC 
     2461 AGGTGGCCGG ACACCCGGCC GGGAGCACTT ACTCAGAGCA AGACATCTTG CGACAGGAAC 
     2521 TGAACACTCG TTTTTTGGCC TCTCAGAGTG CTGACCGCGG GGCTTCCCTG GGCCCTCCGC 
     2581 CCTACCTGCG GACCGAGTTC CATCAGCACC AGCACCAGCA CCAGCACACC CACCAGCACA 
     2641 CGCACCAGCA CACCTTCACG CCGTTCCCCC ACGCCATCCC ACCCACCGCC ATCATGCCGA 
     2701 CGCCAGCACC TCCCATGTTT GACAAATACC CTACAAAAGT TGACCCATTC TACCGGCACA 
     2761 GTCTCTTCCA TTCCTATCCT CCTGCAGTGT CGGGCATCCC CCCTATGATC CCACCCACTG 
     2821 GCCCTTTTGG TTCACTACAA GGAGCATTTC AGCCGAAGTT GACAGATCCT TTCAGACCTA 
     2881 TGTTAAGGAA ACCAGGGAAG TGGTGTGCTA TGCATGTTCA CATCGCCTGG CAGATTTACC 
     2941 ACCACCAACA GAAAGTCAAG AAACAGATGC AGTCAGACCC ACATAAGCTG GACTTTGGAC 
     3001 TGAAACCTGA GTTCCTGAGC CGCCCTCCAG GCCCCAGTCT TTTTGGAGCC ATCCACCACC 
     3061 CCCATGACCT GGCACGGCCT TCAACTTTGT TCTCTGCCGC TGGTGCTGCA CACCCAACTG 
     3121 GGACCCCTTT TGGGCCACCT CCTCATCACA GCAACTTCCT CAACCCTGCT GCCCACCTAG 
     3181 AGCCTTTTAA TCGGCCGTCT ACATTCACAG GCCTAGCAGC AGTTGGTGGC AATGCCTTCG 
     3241 GGGGACTTGG AAATCCTTCC GTTACACCCA ACTCAATGTT CGGCCACAAG GATGGCCCCA 
     3301 GTGTGCAGAA CTTTAGCAAC CCTCACGAAC CCTGGAACCG GCTGCACCGA ACGCCTCCGT 
     3361 CGTTCCCGAC CCCTCCGCCC TGGCTGAAGC CAGGGGAGCT GGAGCGCAGC GCGTCCGCTG 
     3421 CAGCTCATGA CAGAGATAGA GATGTAGATA AACGAGACTC ATCTGTTAGT AAAGATGACA 
     3481 AAGAAAGGGA AAGCGTCGAG AAGAGACACT CCAGCCACCC TTCACCAGCA CCTGTCCTCC 
     3541 CGGTGAATGC CCTGGGACAT ACCCGCAGCT CCACTGAACA GATCCGGGCT CATCTGAACA 
     3601 CTGAGGCTCG GGAGAAGGAC AAACCCAAAG AGAGGGAGAG AGACCACTCG GAATCCCGCA 
     3661 AGGACCTGGC CGCCGACGAG CACAAGGCGA AAGAGGGCCA CCTGCCCGAG AAGGACGGGC 
     3721 ACGGCCACGA GGGGCGCGCC GCGGGCGAAG AGGCCAAGCA GCTGGCCCGG GTGCCGTCTC 
     3781 CCTACGTGCG GACCCCGGTG GTGGAGAGTG CCAGGCCCAA CAGCACCTCG AGCCGGGAGG 
     3841 CCGAGCCGCG CAAGGGTGAG CCGGCCTACG AGAACCCCAA GAAGAGCTCC GAGGTCAAGG 
     3901 TGAAGGAGGA GCGGAAGGAA GACCATGACC TGCCTCCAGA GGCCCCGCAG ACCCACCGGG 
     3961 CCTCGGAGCC GCCGCCTCCC AACTCCTCGT CCAGCGTGCA CCCGGGGCCC CTGGCCTCGA 
     4021 TGCCCATGAC GGTGGGGGTG ACGGGCATTC ACCCCATGAA CAGCATCAGC AGCCTGGACA 
     4081 GGACTCGCAT GATGACCCCC TTCATGGGCA TCAGCCCCCT CCCGGGCGGA GAGCGCTTCC 
     4141 CGTACCCTTC TTTCCACTGG GACCCCATCC GGGACCCCTT GAGGGATCCT TACCGAGAAC 
     4201 TTGACATTCA CCGGAGAGAC CCGCTGGGCA GGGACTTCCT GCTAAGGAAC GACCCGCTCC 
     4261 ACCGGCTCTC GACTCCCCGG CTGTACGAAG CCGACCGCTC CTTCAGGGAC CGGGAGCCTC 
     4321 ACGACTACAG CCACCACCAC CACCACCACC ACCACCCGCT GTCTGTGGAC CCTCGGCGGG 
     4381 AGCACGAGCG GGGAGGCCAC CTGGACGAGC GGGAGCGCTT GCACATGCTC AGAGAAGACT 
     4441 ACGAGCACAC GCGGCTCCAC TCCGTGCACC CCGCCTCCCT CGACGGACAC CTCCCCCACC 
     4501 CCAGCCTCAT CACCCCGGGA CTCCCCAGCA TGCACTATCC CCGCATCAGC CCCACCGCGG 
     4561 GCAACCAGAA CGGACTCCTC AACAAGACCC CTCCGACAGC AGCGCTGAGC GCACCTCCCC 
     4621 CGCTCATCTC CACGCTGGGG GGCCGCCCGG TCTCTCCCAG AAGGACGACT CCTCTGTCCG 
     4681 CAGAGATAAG GGAGAGGCCC CCTTCCCACA CGCTGAAGGA TATCGAGGCC CGAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//