LOCUS       RC226356                8457 bp    DNA     circular  SYN 
DEFINITION  AGAP2 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 1
ACCESSION   NM_001122772
VERSION     NM_001122772.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="AGAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGGGGC GCGGGCGCGC TTCAGCGCCG GACAACGACC TACCTCATCT 
     1081 CGCTGACCCT GGTTAAGCTC GAGTCGGTGC CTCCGCCGCC GCCTTCTCCG TCTGCGGCCG 
     1141 CGGCCGGCGC CGCCGGTGCC AGAGGCTCCG AGACTGGGGA TCCTGGCAGC CCCCGAGGCG 
     1201 CGGAGGAGCC GGGCAAGAAG CGGCACGAAC GTCTCTTCCA CCGGCAGGAT GCGCTGTGGA 
     1261 TCAGCACGAG CAGCGCGGGC ACCGGGGGCG CGGAGCCCCC AGCCCTGTCC CCGGCTCCGG 
     1321 CCAGTCCGGC CCGCCCAGTC TCCCCCGCTC CCGGCCGCCG CCTCTCCCTC TGGGCCGTCC 
     1381 CTCCGGGACC CCCGCTCTCC GGGGGACTGA GCCCCGACCC CAAGCCTGGG GGCGCCCCCA 
     1441 CCTCCTCCCG GCGCCCCCTG CTCAGCAGCC CGAGCTGGGG CGGCCCGGAG CCCGAAGGCC 
     1501 GGGCGGGCGG CGGCATCCCT GGCTCATCCT CTCCGCACCC TGGCACCGGC AGCCGGAGGC 
     1561 TCAAGGTGGC GCCTCCTCCG CCGGCTCCCA AGCCTTGCAA GACCGTGACC ACGAGTGGAG 
     1621 CCAAAGCCGG CGGGGGCAAG GGCGCGGGTA GCCGCCTGTC ATGGCCCGAA AGCGAGGGCA 
     1681 AGCCCAGGGT CAAGGGGTCA AAGAGCAGCG CCGGGACTGG AGCTTCGGTC TCTGCCGCCG 
     1741 CCACCGCCGC CGCCGCCGGG GGAGGGGGCT CTACAGCTTC GACCTCTGGT GGGGTCGGGG 
     1801 CTGGGGCTGG AGCCCGAGGG AAGTTGTCCC CTCGGAAAGG CAAGAGTAAG ACCTTGGACA 
     1861 ACAGTGACTT GCATCCGGGA CCGCCTGCCG GCTCTCCTCC TCCGCTAACC CTCCCACCAA 
     1921 CTCCGAGTCC AGCCACTGCT GTCACCGCTG CTTCCGCGCA GCCCCCCGGG CCTGCACCTC 
     1981 CAATCACTCT GGAGCCTCCA GCTCCGGGGC TGAAACGGGG CCGGGAGGGG GGCCGAGCAT 
     2041 CCACTCGTGA CCGCAAGATG CTCAAGTTTA TCAGCGGCAT CTTCACCAAG AGCACAGGAG 
     2101 GGCCTCCTGG CTCCGGGCCC CTTCCCGGAC CCCCCAGCCT GTCTTCTGGC AGCGGGTCCA 
     2161 GGGAGCTGCT GGGCGCCGAG CTCCGCGCTT CCCCTAAGGC TGTGATCAAT AGCCAGGAAT 
     2221 GGACTTTGAG CCGCTCCATT CCTGAACTGC GCCTGGGTGT GCTGGGCGAT GCCAGGAGTG 
     2281 GGAAGTCATC GCTCATCCAC CGATTCCTGA CTGGCTCATA CCAGGTGCTG GAGAAGACAG 
     2341 AGAGTGAGCA GTACAAGAAA GAAATGTTGG TGGATGGACA GACACATCTG GTGCTAATCC 
     2401 GAGAGGAAGC TGGGGCACCT GATGCCAAGT TCTCAGGCTG GGCAGATGCT GTGATCTTCG 
     2461 TCTTCAGCCT GGAGGATGAG AACAGTTTCC AGGCTGTGAG CCGTCTCCAT GGGCAGCTGA 
     2521 GTTCCCTTCG CGGGGAGGGA CGAGGAGGCC TGGCCTTGGC ACTGGTGGGG ACACAAGACA 
     2581 GGATCAGTGC TTCCTCCCCT CGGGTGGTGG GAGATGCTCG TGCCAGAGCT CTGTGCGCGG 
     2641 ACATGAAACG CTGCAGCTAC TATGAGACTT GTGCAACCTA TGGGCTCAAT GTGGATCGGG 
     2701 TCTTCCAGGA GGTGGCCCAG AAGGTGGTGA CCTTGCGCAA GCAGCAACAG CTTCTGGCTG 
     2761 CCTGCAAGTC CCTGCCCAGC TCCCCAAGCC ACTCAGCTGC ATCCACTCCG GTAGCTGGCC 
     2821 AGGCTAGTAA CGGGGGCCAC ACTAGCGACT ACTCTTCTTC CCTCCCGTCC TCACCGAATG 
     2881 TTGGTCACCG GGAGCTCCGA GCCGAGGCAG CTGCAGTGGC TGGATTGAGC ACCCCAGGGT 
     2941 CCCTGCACCG GGCAGCCAAG CGCAGGACCA GCCTTTTTGC GAATCGTCGG GGTAGTGACT 
     3001 CCGAGAAACG AAGCTTGGAT AGTCGGGGAG AGACAACAGG GAGTGGGCGA GCCATCCCCA 
     3061 TCAAACAGAG CTTCCTACTA AAACGAAGTG GCAATTCCTT GAACAAAGAA TGGAAGAAGA 
     3121 AATATGTAAC CCTGTCCAGT AATGGCTTTC TACTCTACCA CCCCAGTATT AACGATTACA 
     3181 TCCACAGTAC CCACGGCAAG GAGATGGACT TGCTGCGAAC AACAGTCAAA GTCCCGGGCA 
     3241 AGCGGCCCCC GAGGGCCATC TCTGCCTTTG GCCCCTCAGC CAGCATTAAC GGGCTCGTCA 
     3301 AGGACATGAG CACTGTCCAG ATGGGTGAAG GCCTGGAAGC CACTACTCCC ATGCCAAGCC 
     3361 CTAGCCCCAG CCCCAGTTCC CTGCAGCCAC CACCAGATCA GACATCCAAA CACCTGCTGA 
     3421 AGCCAGACCG GAATTTGGCC CGAGCCCTCA GCACGGACTG TACCCCATCT GGAGACCTGA 
     3481 GCCCCCTGAG TCGGGAACCC CCTCCTTCTC CCATGGTGAA GAAGCAGAGG AGGAAAAAAT 
     3541 TGACAACACC ATCCAAGACT GAAGGCTCGG CTGGGCAGGC TGAAGCCAAG CGCAAAATGT 
     3601 GGAAACTAAA ATCCTTTGGT AGTTTAAGAA ATATTTATAA AGCAGAGGAA AACTTTGAGT 
     3661 TCCTGATCGT GTCCAGCACG GGTCAGACGT GGCACTTTGA GGCAGCCAGT TTTGAGGAGC 
     3721 GGGATGCCTG GGTCCAGGCC ATCGAGAGTC AGATCCTAGC CAGTCTGCAA TGCTGTGAGA 
     3781 GCAGCAAGGT CAAGCTGCGC ACAGACAGCC AAAGCGAGGC CGTGGCCATC CAGGCGATCC 
     3841 GGAACGCCAA GGGGAATTCA ATCTGCGTGG ACTGCGGGGC CCCCAACCCC ACGTGGGCCA 
     3901 GCTTGAACCT GGGCGCCCTC ATCTGCATCG AGTGTTCTGG CATCCACCGC AACCTGGGCA 
     3961 CACACCTGTC CCGCGTTCGC TCGCTGGACT TGGACGACTG GCCACGGGAG CTGACCCTGG 
     4021 TGCTGACGGC TATTGGCAAC GACACGGCCA ACCGCGTGTG GGAAAGCGAC ACGCGAGGCC 
     4081 GTGCCAAGCC CTCGCGGGAC TCTTCGCGGG AGGAGCGCGA GTCGTGGATT CGCGCCAAGT 
     4141 ACGAGCAGCT ACTGTTCCTG GCGCCGCTGA GCACCTCGGA GGAGCCGCTG GGCCGCCAGC 
     4201 TGTGGGCCGC CGTGCAGGCC CAGGACGTGG CTACCGTTCT CCTGCTTTTG GCCCATGCGC 
     4261 GACACGGGCC GCTCGACACC AGCGTAGAGG ACCCACAGCT GCGCTCCCCA CTCCACCTGG 
     4321 CGGCCGAGCT CGCCCACGTC GTCATCACGC AACTGCTGCT GTGGTACGGC GCGGACGTGG 
     4381 CGGCCCGTGA CGCCCAGGGC CGCACGGCGC TGTTCTACGC CCGCCAGGCT GGAAGCCAGC 
     4441 TGTGCGCCGA CATCCTTCTC CAGCACGGCT GCCCGGGTGA GGGCGGCAGC GCGGCCACCA 
     4501 CGCCCAGCGC GGCCACCACG CCCAGCATCA CCGCCACGCC CAGCCCCCGC CGCCGGAGCA 
     4561 GCGCCGCTAG CGTGGGCCGC GCCGACGCCC CGGTTGCGCT GGTAACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//