LOCUS       RC226348                8394 bp    DNA     circular  SYN 
DEFINITION  HIPK2 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 2
ACCESSION   NM_001113239
VERSION     NM_001113239.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="HIPK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGTG TACGAAGGTA TGGCCTCACA TGTGCAAGTT TTCTCCCCTC 
     1081 ACACCCTTCA ATCAAGTGCC TTCTGTAGTG TGAAGAAACT GAAAATAGAG CCGAGTTCCA 
     1141 ACTGGGACAT GACTGGGTAC GGCTCCCACA GCAAAGTGTA TAGCCAGAGC AAGAACATCC 
     1201 CCCTGTCGCA GCCAGCCACC ACAACCGTCA GCACCTCCTT GCCGGTCCCA AACCCAAGCC 
     1261 TACCTTACGA GCAGACCATC GTCTTCCCAG GAAGCACCGG GCACATCGTG GTCACCTCAG 
     1321 CAAGCAGCAC TTCTGTCACC GGGCAAGTCC TCGGCGGACC ACACAACCTA ATGCGTCGAA 
     1381 GCACTGTGAG CCTCCTTGAT ACCTACCAAA AATGTGGACT CAAGCGTAAG AGCGAGGAGA 
     1441 TCGAGAACAC AAGCAGCGTG CAGATCATCG AGGAGCATCC ACCCATGATT CAGAATAATG 
     1501 CAAGCGGGGC CACTGTCGCC ACTGCCACCA CGTCTACTGC CACCTCCAAA AACAGCGGCT 
     1561 CCAACAGCGA GGGCGACTAT CAGCTGGTGC AGCATGAGGT GCTGTGCTCC ATGACCAACA 
     1621 CCTACGAGGT CTTAGAGTTC TTGGGCCGAG GGACGTTTGG GCAAGTGGTC AAGTGCTGGA 
     1681 AACGGGGCAC CAATGAGATC GTAGCCATCA AGATCCTGAA GAACCACCCA TCCTATGCCC 
     1741 GACAAGGTCA GATTGAAGTG AGCATCCTGG CCCGGTTGAG CACGGAGAGT GCCGATGACT 
     1801 ATAACTTCGT CCGGGCCTAC GAATGCTTCC AGCACAAGAA CCACACGTGC TTGGTCTTCG 
     1861 AGATGTTGGA GCAGAACCTC TATGACTTTC TGAAGCAAAA CAAGTTTAGC CCCTTGCCCC 
     1921 TCAAATACAT TCGCCCAGTT CTCCAGCAGG TAGCCACAGC CCTGATGAAA CTCAAAAGCC 
     1981 TAGGTCTTAT CCACGCTGAC CTCAAACCAG AAAACATCAT GCTGGTGGAT CCATCTAGAC 
     2041 AACCATACAG AGTCAAGGTC ATCGACTTTG GTTCAGCCAG CCACGTCTCC AAGGCTGTGT 
     2101 GCTCCACCTA CTTGCAGTCC AGATATTACA GGGCCCCTGA GATCATCCTT GGTTTACCAT 
     2161 TTTGTGAGGC AATTGACATG TGGTCCCTGG GCTGTGTTAT TGCAGAATTG TTCCTGGGTT 
     2221 GGCCGTTATA TCCAGGAGCT TCGGAGTATG ATCAGATTCG GTATATTTCA CAAACACAGG 
     2281 GTTTGCCTGC TGAATATTTA TTAAGCGCCG GGACAAAGAC AACTAGGTTT TTCAACCGTG 
     2341 ACACGGACTC ACCATATCCT TTGTGGAGAC TGAAGACACC AGATGACCAT GAAGCAGAGA 
     2401 CAGGGATTAA GTCAAAAGAA GCAAGAAAGT ACATTTTCAA CTGTTTAGAT GATATGGCCC 
     2461 AGGTGAACAT GACGACAGAT TTGGAAGGGA GCGACATGTT GGTAGAAAAG GCTGACCGGC 
     2521 GGGAGTTCAT TGACCTGTTG AAGAAGATGC TGACCATTGA TGCTGACAAG AGAATCACTC 
     2581 CAATCGAAAC CCTGAACCAT CCCTTTGTCA CCATGACACA CTTACTCGAT TTTCCCCACA 
     2641 GCACACACGT CAAATCATGT TTCCAGAACA TGGAGATCTG CAAGCGTCGG GTGAATATGT 
     2701 ATGACACGGT GAACCAGAGC AAAACCCCTT TCATCACGCA CGTGGCCCCC AGCACGTCCA 
     2761 CCAACCTGAC CATGACCTTT AACAACCAGC TGACCACTGT CCACAACCAG CCCTCAGCGG 
     2821 CATCCATGGC TGCAGTGGCC CAGCGGAGCA TGCCCCTGCA GACAGGAACA GCCCAGATTT 
     2881 GTGCCCGGCC TGACCCGTTC CAGCAAGCTC TCATCGTGTG TCCCCCCGGC TTCCAAGGCT 
     2941 TGCAGGCCTC TCCCTCTAAG CACGCTGGCT ACTCGGTGCG AATGGAAAAT GCAGTTCCCA 
     3001 TCGTCACTCA AGCCCCAGGA GCTCAGCCTC TTCAGATCCA ACCAGGTCTG CTTGCCCAGC 
     3061 AGGCTTGGCC AAGTGGGACC CAGCAGATCC TGCTTCCCCC AGCATGGCAG CAACTGACTG 
     3121 GAGTGGCCAC CCACACATCA GTGCAGCATG CCACCGTGAT TCCCGAGACC ATGGCAGGCA 
     3181 CCCAGCAGCT GGCGGACTGG AGAAATACGC ATGCTCACGG AAGCCATTAT AATCCCATCA 
     3241 TGCAGCAGCC TGCACTATTG ACCGGTCATG TGACCCTTCC AGCAGCACAG CCCTTAAATG 
     3301 TGGGTGTGGC CCACGTGATG CGGCAGCAGC CAACCAGCAC CACCTCCTCC CGGAAGAGTA 
     3361 AGCAGCACCA GTCATCTGTG AGAAATGTCT CCACCTGTGA GGTGTCCTCC TCTCAGGCCA 
     3421 TCAGCTCCCC ACAGCGATCC AAGCGTGTCA AGGAGAACAC ACCTCCCCGC TGTGCCATGG 
     3481 TGCACAGTAG CCCGGCCTGC AGCACCTCGG TCACCTGTGG GTGGGGCGAC GTGGCCTCCA 
     3541 GCACCACCCG GGAACGGCAG CGGCAGACAA TTGTCATTCC CGACACTCCC AGCCCCACGG 
     3601 TCAGCGTCAT CACCATCAGC AGTGACACGG ACGAGGAGGA GGAACAGAAA CACGCCCCCA 
     3661 CCAGCACTGT CTCCAAGCAA AGAAAAAACG TCATCAGCTG TGTCACAGTC CACGACTCCC 
     3721 CCTACTCCGA CTCCTCCAGC AACACCAGCC CCTACTCCGT GCAGCAGCGT GCTGGGCACA 
     3781 ACAATGCCAA TGCCTTTGAC ACCAAGGGGA GCCTGGAGAA TCACTGCACG GGGAACCCCC 
     3841 GAACCATCAT CGTGCCACCC CTGAAAACCC AGGCCAGCGA AGTATTGGTG GAGTGTGATA 
     3901 GCCTGGTGCC AGTCAACACC AGTCACCACT CGTCCTCCTA CAAGTCCAAG TCCTCCAGCA 
     3961 ACGTGACCTC CACCAGCGGT CACTCTTCAG GGAGCTCATC TGGAGCCATC ACCTACCGGC 
     4021 AGCAGCGGCC GGGCCCCCAC TTCCAGCAGC AGCAGCCACT CAATCTCAGC CAGGCTCAGC 
     4081 AGCACATCAC CACGGACCGC ACTGGGAGCC ACCGAAGGCA GCAGGCCTAC ATCACTCCCA 
     4141 CCATGGCCCA GGCTCCGTAC TCCTTCCCGC ACAACAGCCC CAGCCACGGC ACTGTGCACC 
     4201 CGCATCTGGC TGCAGCCGCT GCCGCTGCCC ACCTCCCCAC CCAGCCCCAC CTCTACACCT 
     4261 ACACTGCGCC GGCGGCCCTG GGCTCCACCG GCACCGTGGC CCACCTGGTG GCCTCGCAAG 
     4321 GCTCTGCGCG CCACACCGTG CAGCACACTG CCTACCCAGC CAGCATCGTC CACCAGGTCC 
     4381 CCGTGAGCAT GGGCCCCCGG GTCCTGCCCT CGCCCACCAT CCACCCGAGT CAGTATCCAG 
     4441 CCCAATTTGC CCACCAGACC TACATCAGCG CCTCGCCAGC CTCCACCGTC TACACTGGAT 
     4501 ACCCACTGAG CCCCGCCAAG GTCAACCAGT ACCCTTACAT AACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//