LOCUS       RC226338                8289 bp    DNA     circular  SYN 
DEFINITION  ARAP1 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4
ACCESSION   NM_001135190
VERSION     NM_001135190.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4444..4535 
                     /label="Myc-DDK"  
     misc_feature    4565..5150 
                     /label="PolyA signal"  
     misc_feature    4622..4641 
                     /label="Restriction Enzyme"  
     misc_feature    5426..6098 
                     /label="ColE1"  
     misc_feature    6528..7322 
                     /label="Kan/Neo"  
     misc_feature    7357..7659 
                     /label="SV40 ori"  
     misc_feature    7745..8222 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4426 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="ARAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAAGAAG GAGGAGCCCC CACCGAGCCG AGTCCCACGG GCCGTGCGCG 
     1081 TGGCCAGTCT GCTGAGCGAG GGAGAGGAAC TGTCTGGGGA CGACCAAGGG GATGAGGAAG 
     1141 AGGATGACCA CGCCTATGAG GGCGTCCCCA ATGGCGGATG GCATACCAGC AGCCTGAGCT 
     1201 TGTCCTTGCC CAGCACAATA GCTGCGCCAC ACCCCATGGA CGGGCCGCCT GGGGGCTCCA 
     1261 CCCCCGTCAC ACCAGTCATC AAGGCTGGCT GGCTGGACAA GAACCCACCG CAGGGATCTT 
     1321 ACATCTATCA GAAACGATGG GTGAGACTGG ATACTGATCA CCTGCGATAC TTTGACAGTA 
     1381 ACAAGGACGC TTACTCTAAG CGCTTTATCT CTGTGGCCTG CATCTCCCAC GTGGCTGCCA 
     1441 TCGGGGACCA GAAGTTTGAA GTGATCACAA ACAACCGAAC CTTTGCCTTC CGGGCAGAGA 
     1501 GTGATGTGGA GCGGAAGGAG TGGATGCAGG CCCTGCAGCA GGCCATGGCT GAGCAGCGTG 
     1561 CCCGGGCCCG GCTCTCTAGC GCTTATCTGC TGGGAGTTCC AGGCTCAGAG CAGCCTGACC 
     1621 GCGCTGGCAG CCTGGAGCTT CGTGGCTTCA AGAATAAGCT GTACGTGGCC GTGGTCGGGG 
     1681 ACAAAGTGCA GCTCTACAAG AATCTAGAGG AGTACCACCT GGGCATTGGC ATCACCTTCA 
     1741 TCGACATGAG CGTGGGCAAC GTGAAGGAAG TGGACCGGCG CAGCTTCGAC CTCACCACGC 
     1801 CCTACCGCAT CTTCAGCTTC TCTGCTGACT CAGAGCTAGA GAAGGAGCAG TGGTTGGAGG 
     1861 CCATGCAGGG AGCCATCGCT GAGGCCCTGT CTACCTCGGA GGTGGCCGAG CGCATCTGGG 
     1921 CTGCAGCCCC CAACAGGTTC TGTGCTGACT GCGGGGCTCC TCAGCCTGAC TGGGCCTCCA 
     1981 TCAACCTCTG TGTTGTTATC TGCAAGCGCT GTGCAGGGGA GCACCGTGGC CTGGGCGCTG 
     2041 GCGTCTCCAA GGTGCGGAGC CTGAAGATGG ACAGGAAGGT GTGGACAGAA ACACTTATCG 
     2101 AGGCCCTGTG TGCTGCAGTC ACCACCACAG ACCTGGCTGA GACCCAGGCG CTCCTGGGCT 
     2161 GTGGGGCTGG GATCAACTGC TTCTCGGGGG ACCCTGAGGC CCCCACGCCC CTGGCTCTTG 
     2221 CAGAGCAGGC GGGGCAGACG CTGCAGATGG AATTCCTTCG GAACAACCGG ACCACAGAGG 
     2281 TGCCTCGGCT GGACTCGATG AAGCCCCTGG AAAAGCACTA CTCAGTTGTC CTGCCGACCG 
     2341 TGAGCCACAG TGGCTTCCTC TACAAGACTG CCTCTGCCGG CAAGCTGCTA CAGGACCGCC 
     2401 GGGCCCGGGA AGAGTTCAGC CGGCGCTGGT GTGTCCTTGG TGACGGGGTC CTGAGCTACT 
     2461 TTGAGAATGA GCGGGCAGTG ACCCCCAATG GAGAGATTCG GGCCAGCGAG ATTGTGTGCC 
     2521 TGGCAGTGCC CCCTCCTGAC ACCCATGGCT TTGAGCACAC CTTTGAGGTG TACACGGAGG 
     2581 GAGAACGGCT GTACCTGTTT GGGCTGGAGA GTGCGGAGCA GGCTCATGAG TGGGTCAAGT 
     2641 GTATTGCTAA GGCATTCGTG CCTCCCCTAG CCGAGGATCT GCTGGCCCGG GATTTTGAGC 
     2701 GGCTGGGACG CCTACCCTAC AAAGCTGGCC TGAGCCTACA GCGGGCCCAG GAGGGCTGGT 
     2761 TCTCTCTCAG TGGCTCGGAG CTCCGTGCTG TCTTCCCGGA GGGGCCCTGC GAAGAGCCGC 
     2821 TGCAACTACG GAAACTGCAG GAGCTTTCCA TCCAGGGGGA CAGTGAGAAC CAGGTGCTGG 
     2881 TGCTGGTGGA GCGAAGGAGG ACACTGTACA TACAGGGCGA GCGGCGGCTG GACTTCATGG 
     2941 GTTGGCTGGG GGCCATCCAG AAAGCAGCCG CCAGCATGGG GGACACGCTG TCGGAGCAGC 
     3001 AGCTTGGGGA CTCGGATATC CCGGTGATCG TGTACCGCTG TGTGGACTAC ATCACGCAGT 
     3061 GCGGCCTGAC CTCCGAGGGC ATCTACCGCA AGTGTGGGCA GACATCGAAG ACACAGCGGC 
     3121 TGCTGGAGAG CCTGCGGCAG GATGCGCGCT CTGTGCACCT CAAGGAGGGC GAGCAGCACG 
     3181 TGGATGATGT TTCCTCGGCG CTCAAGCGCT TCCTGCGCGA CCTGCCTGAT GGGCTCTTCA 
     3241 CTCGCGCCCA GCGCCTAACC TGGCTGGAGG CCTCAGAGAT TGAGGACGAG GAGGAGAAGG 
     3301 TCTCCAGGTA CCGAGAGCTG CTGGTGCGGC TGCCCCCTGT CAACCGGGCC ACAGTGAAGG 
     3361 CCCTTATCAG CCACCTGTAC TGTGTTCAGT GCTTCTCAGA CACGAACCAG ATGAACGTGC 
     3421 ACAACCTGGC AATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG GACTACAAGG 
     3481 CTGGCCGTGT GGTGGAAGAC CTCATTAACC ACTATGTGGT GGTGTTTAGT GTGGATGAGG 
     3541 AAGAGCTCAG GAAGCAGCGG GAGGAGATCA CTGCCATTGT GAAGATGCGC GTGGCTGGCA 
     3601 CTGCCAGTGG GACCCAGCAT GCCGGTGACT TCATCTGCAC AGTGTATCTG GAAGAGAAGA 
     3661 AGGCAGAGAC TGAGCAGCAT ATCAAGGTCC CAGCATCCAT GACTGCTGAG GAGCTCACCC 
     3721 TGGAGATCCT GGATCGCCGG AACGTGGGCA TCAGGGAGAA GGACTATTGG ACCTGCTTTG 
     3781 AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG GTGCTGCCCA 
     3841 TCCTGCACGG GCTGGGCACG GACAGCCACC TGGTGGTGAA GAAGCACCAG GCCATGGAGG 
     3901 CCATGCTGCT GTACCTGGCC AGCCGTGTCG GTGACACCAA GCATGGCATG ATGAAGTTCC 
     3961 GTGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCTCAGG TGGCTTCCAC GATCGCTACT 
     4021 TCATCCTCAA CAGCAGCTGC TTGCGGCTCT ACAAGGAGGT CCGGAGTCAC CGGCCTGAGA 
     4081 AGGAGTGGCC TATTAAGAGT CTCAAAGTCT ACCTGGGAGT GAAGAAGAAA CTCAGGCCAC 
     4141 CCACCTGCTG GGGCTTCACA GTGGTGCATG AGACAGAGAA ACATGAGAAG CAGCAGTGGT 
     4201 ACCTCTGCTG TGACACACAG ATGGAGCTCC GGGAGTGGTT CGCTACCTTT CTGTTTGTGC 
     4261 AGCATGACGG CCTGGTGTGG CCCTCAGAGC CCTCACGCGT GTCCCGGGCA GTGCCTGAGG 
     4321 TCCGGCTGGG TAGTGTGTCA CTGATCCCCC TTCGAGGTAG TGAAAATGAA ATGCGCCGGA 
     4381 GTGTGGCTGC CTTCACCGCG GACCCTCTGT CTCTTCTGCG CAACGTCAGC GGACCGACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//