LOCUS       RC226334                8229 bp    DNA     circular  SYN 
DEFINITION  MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 1
ACCESSION   NM_001105077
VERSION     NM_001105077.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4386..4477 
                     /label="Myc-DDK"  
     misc_feature    4507..5092 
                     /label="PolyA signal"  
     misc_feature    4564..4583 
                     /label="Restriction Enzyme"  
     misc_feature    5368..6040 
                     /label="ColE1"  
     misc_feature    6470..7264 
                     /label="Kan/Neo"  
     misc_feature    7299..7601 
                     /label="SV40 ori"  
     misc_feature    7687..8164 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4368..4374 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4368 
                     /label="MECOM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCTTAGAC GAATTTTACA ATGTGAAGTT CTGCATAGAT GCCAGTCAAC 
     1081 CAGATGTTGG AAGCTGGCTC AAGTACATTA GATTCGCTGG CTGTTATGAT CAGCACAACC 
     1141 TTGTTGCATG CCAGATAAAT GATCAGATAT TCTATAGAGT AGTTGCAGAC ATTGCGCCGG 
     1201 GAGAGGAGCT TCTGCTGTTC ATGAAGAGCG AAGACTATCC CCATGAAACT ATGGCGCCGG 
     1261 ATATCCACGA AGAACGGCAA TATCGCTGCG AAGACTGTGA CCAGCTCTTT GAATCTAAGG 
     1321 CTGAACTAGC AGATCACCAA AAGTTTCCAT GCAGTACTCC TCACTCAGCA TTTTCAATGG 
     1381 TTGAAGAGGA CTTTCAGCAA AAACTCGAAA GCGAGAATGA TCTCCAAGAG ATACACACGA 
     1441 TCCAGGAGTG TAAGGAATGT GACCAAGTTT TTCCTGATTT GCAAAGCCTG GAGAAACACA 
     1501 TGCTGTCACA TACTGAAGAG AGGGAATACA AGTGTGATCA GTGTCCCAAG GCATTTAACT 
     1561 GGAAGTCCAA TTTAATTCGC CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG 
     1621 AAAACTGTGC CAAGCAGGTT TTCACGGACC CTAGCAACCT TCAGCGGCAC ATTCGCTCTC 
     1681 AGCATGTCGG TGCCCGGGCC CATGCATGCC CGGAGTGTGG CAAAACGTTT GCCACTTCGT 
     1741 CGGGCCTCAA ACAACACAAG CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT 
     1801 GCCATAAATC CTATACTCAG TTTTCAAACC TTTGCCGTCA TAAGCGCATG CATGCTGATT 
     1861 GCAGAACCCA AATCAAGTGC AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA 
     1921 ATAAACACAG GAGGTTTTGT GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC 
     1981 AAGGCATTTC ACTTCCTGGA ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC 
     2041 ATGCCAACCC GGGCCTTGCT GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT 
     2101 TTCCAACAGC TCCTGGATTT TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC 
     2161 ACAGGCCTCC TTTGATACCT GCTAGTTCTC CTGTTAAAGG ACTATCAAGT ACTGAACAGA 
     2221 CAAACAAAAG TCAAAGTCCC CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA 
     2281 TTTTGAAGGC ACTATCTAAA CACCCATCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC 
     2341 CCGAGAGGTC CTCTGAAGAG AGGCCCTTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG 
     2401 ACCTTGATGA TGTCAGTACA CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC 
     2461 TGGAAAGTGA CATTGAAAGT GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG 
     2521 ACAAAGTAAG CCCTCTTCAG AATCTGGCTT CAATAAATAA TAAGAAAGAA TACAGCAATC 
     2581 ATTCCATTTT CTCACCATCT TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT 
     2641 CTATAAAGGC TATTGCTTCT ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC 
     2701 TGCAAGACAA AAAAGTTGGA GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC 
     2761 CAGCATTCTC TCAATCAATG TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA 
     2821 AAATGGAACC CCAATCACCA GGTGAAGTAA AGAAACTGCA GAAGGGCAGC TCTGAGTCCC 
     2881 CCTTTGATCT CACCACTAAG CGAAAGGATG AGAAGCCCTT GACTCCAGTC CCCTCCAAGC 
     2941 CTCCAGTGAC ACCTGCCACA AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA 
     3001 GTAGAGCCAG TGGGACAAAG CTGACTGAGC CTCGAAAAAA CCACGTGTTT GGGGGAAAAA 
     3061 AAGGAAGCAA CGTCGAATCA AGACCTGCTT CAGATGGTTC CTTGCAGCAT GCAAGACCCA 
     3121 CTCCTTTCTT TATGGACCCT ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG 
     3181 AAGCTTTAAA AGAGAAATAC TTGAGGCCTT CTCCAGGATT CTTGTTTCAC CCACAATTCC 
     3241 AACTGCCTGA TCAGAGAACT TGGATGTCAG CTATTGAAAA CATGGCAGAA AAGCTAGAGA 
     3301 GCTTCAGTGC CCTGAAACCT GAGGCCAGTG AGCTCTTACA GTCAGTGCCC TCTATGTTCA 
     3361 ACTTCAGGGC GCCTCCCAAT GCCCTGCCAG AGAACCTTCT GCGGAAGGGA AAGGAGCGCT 
     3421 ATACCTGCAG ATACTGTGGC AAGATTTTTC CAAGGTCTGC AAACCTAACA CGGCACTTGA 
     3481 GAACCCACAC AGGAGAGCAG CCTTACAGAT GCAAATACTG TGACAGATCA TTTAGCATAT 
     3541 CTTCTAACTT GCAAAGGCAT GTTCGCAACA TCCACAATAA AGAGAAGCCA TTTAAGTGTC 
     3601 ACTTATGTGA TAGGTGTTTT GGTCAACAAA CCAATTTAGA CAGACACCTA AAGAAACATG 
     3661 AGAATGGGAA CATGTCCGGT ACAGCAACAT CGTCGCCTCA TTCTGAACTG GAAAGTACAG 
     3721 GTGCGATTCT GGATGACAAA GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATTGGGA 
     3781 ACAGCAACCA TGGCAGCCAA TCTCCCAGGA ATGTGGAGGA GAGAATGAAT GGCAGTCATT 
     3841 TTAAAGATGA AAAGGCTTTG GTGACCAGTC AAAATTCAGA CTTGCTGGAT GATGAAGAAG 
     3901 TTGAAGATGA GGTGTTGTTA GATGAGGAGG ATGAAGACAA TGATATTACT GGAAAAACAG 
     3961 GAAAGGAACC AGTGACAAGT AATTTACATG AAGGAAACCC TGAGGATGAC TATGAAGAAA 
     4021 CCAGTGCCCT GGAGATGAGT TGCAAGACAT CCCCAGTGAG GTATAAAGAG GAAGAATATA 
     4081 AAAGTGGACT TTCTGCTCTA GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA 
     4141 AAATGGAAGA TAATCAATAT TCTGAAGCTG AGCTGTCTTC TTTTAGTACT TCCCATGTGC 
     4201 CAGAGGAACT TAAGCAGCCG TTACACAGAA AGTCCAAATC GCAGGCATAT GCTATGATGC 
     4261 TGTCACTGTC TGACAAGGAG TCCCTCCATT CTACATCCCA CAGTTCTTCC AACGTGTGGC 
     4321 ACAGTATGGC CAGGGCTGCG GCGGAATCCA GTGCTATCCA GTCCATAAGC CACGTAACGC 
     4381 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4441 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4501 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4561 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4621 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4681 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4741 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4801 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4861 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4921 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4981 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5041 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5101 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5161 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5221 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5281 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5341 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5401 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5461 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5521 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5581 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5641 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5701 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5761 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5821 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5881 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5941 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6001 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6061 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6121 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6181 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6241 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6301 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6361 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6421 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6481 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6541 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6601 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6661 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6721 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6781 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6841 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6901 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6961 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7021 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7081 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7141 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7201 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7261 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7321 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7381 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7441 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7681 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7741 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7801 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7861 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7921 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7981 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8041 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8101 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8161 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8221 GCGAATTTT
//