LOCUS       RC226333                8223 bp    DNA     circular  SYN 
DEFINITION  IQSEC1 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 1 (IQSEC1), transcript variant 1
ACCESSION   NM_001134382
VERSION     NM_001134382.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4380..4471 
                     /label="Myc-DDK"  
     misc_feature    4501..5086 
                     /label="PolyA signal"  
     misc_feature    4558..4577 
                     /label="Restriction Enzyme"  
     misc_feature    5362..6034 
                     /label="ColE1"  
     misc_feature    6464..7258 
                     /label="Kan/Neo"  
     misc_feature    7293..7595 
                     /label="SV40 ori"  
     misc_feature    7681..8158 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4362..4368 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4362 
                     /label="IQSEC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGCAGA AGACGCTATT TCGTCGAGGG CGAGGCCCCC AGCAGTGAGA 
     1081 CTGGCACATC CCTGGACAGC CCCTCAGCCT ACCCCCAGGG CCCCTTGGTG CCCGGTTCCA 
     1141 GCCTGAGCCC GGATCACTAC GAGCACACGT CAGTGGGAGC CTATGGGCTG TACTCGGGGC 
     1201 CGCCGGGGCA ACAGCAGCGC ACGCGGAGGC CCAAGCTGCA GCACTCGACC TCCATCCTGC 
     1261 GCAAGCAGGC TGAGGAGGAG GCCATCAAGC GCTCACGCTC ACTCTCCGAG AGCTATGAGC 
     1321 TCTCCTCGGA CCTGCAGGAC AAGCAGGTGG AGATGCTAGA ACGAAAGTAT GGGGGGCGCC 
     1381 TGGTAACCCG CCATGCGGCC CGCACCATCC AGACGGCGTT TCGCCAGTAC CAGATGAACA 
     1441 AGAACTTCGA GCGCTTGCGC AGCTCCATGT CAGAGAACCG CATGTCACGC CGGATTGTGC 
     1501 TGTCCAACAT GAGGATGCAG TTCTCCTTTG AGGGGCCTGA GAAAGTGCAC AGCTCCTACT 
     1561 TCGAGGGGAA GCAGGTCTCA GTGACTAACG ACGGCTCCCA GCTGGGAGCC CTGGTGTCCC 
     1621 CTGAGTGTGG TGACCTCAGC GAGCCCACCA CCCTCAAGTC TCCGGCCCCC TCCAGTGACT 
     1681 TTGCGGACGC CATCACCGAG CTGGAGGACG CCTTCTCTAG GCAAGTGAAA TCACTGGCCG 
     1741 AGTCCATCGA CGATGCCCTC AACTGCCGCA GCCTGCACAC TGAGGAGGCA CCGGCCCTGG 
     1801 ATGCGGCGCG GGCCCGGGAC ACCGAACCCC AGACAGCCCT GCACGGCATG GACCACCGCA 
     1861 AACTGGACGA GATGACGGCC TCGTACAGTG ATGTCACCCT GTACATCGAT GAGGAGGAGC 
     1921 TGTCGCCCCC TCTGCCCCTC TCGCAGGCAG GGGACCGGCC GTCCAGCACC GAGTCGGACC 
     1981 TGCGGCTACG GGCTGGGGGC GCAGCCCCAG ACTACTGGGC CCTGGCCCAC AAAGAGGACA 
     2041 AGGCTGACAC GGACACGAGC TGCCGGAGCA CGCCGTCGCT GGAGCGGCAG GAGCAGCGGC 
     2101 TGCGGGTGGA GCATCTGCCG CTGCTCACCA TCGAGCCACC CAGCGACAGC TCTGTGGACC 
     2161 TTAGTGACCG CTCGGAGCGG GGGTCACTCA AGAGGCAGAG TGCTTACGAG CGCAGCCTTG 
     2221 GCGGGCAGCA GGGCAGTCCC AAGCATGGTC CCCACAGCGG CGCCCCCAAG AGCCTCCCCC 
     2281 GGGAGGAGCC TGAGTTGCGG CCCCGGCCCC CCAGGCCCCT GGACAGCCAC TTGGCCATCA 
     2341 ATGGCTCAGC CAACCGGCAG AGCAAGTCTG AGTCGGACTA CTCAGACGGT GACAATGACA 
     2401 GCATCAACAG CACGTCCAAC TCCAACGATA CCATCAACTG CAGCTCCGAG TCATCGTCCC 
     2461 GTGACAGCCT GCGGGAGCAG ACGCTCAGCA AGCAGACCTA CCACAAGGAG GCCCGCAACA 
     2521 GCTGGGACTC GCCTGCCTTT AGCAACGATG TCATCCGCAA GAGGCACTAC CGCATCGGCC 
     2581 TGAACCTCTT CAACAAGAAG CCTGAGAAGG GAGTCCAGTA CCTCATCGAG CGTGGCTTTG 
     2641 TGCCCGACAC GCCCGTCGGG GTGGCCCACT TCCTGCTGCA GCGCAAGGGC CTCAGCCGGC 
     2701 AGATGATCGG CGAGTTCCTG GGCAACCGGC AGAAGCAGTT CAACCGTGAC GTGCTCGACT 
     2761 GCGTCGTGGA CGAGATGGAC TTCTCTACCA TGGAGCTGGA TGAGGCCCTC AGGAAATTCC 
     2821 AGGCGCACAT CCGTGTCCAA GGGGAGGCTC AGAAAGTGGA GCGGCTCATA GAGGCGTTCA 
     2881 GCCAGCGCTA CTGCATCTGC AACCCTGGGG TGGTGCGGCA ATTCCGGAAC CCAGACACCA 
     2941 TTTTCATCCT GGCCTTCGCC ATCATCCTGC TGAACACCGA CATGTACAGC CCCAATGTCA 
     3001 AGCCCGAGCG GAAAATGAAG CTAGAGGACT TCATCAAGAA CCTCCGAGGT GTGGACGATG 
     3061 GTGAGGACAT TCCCCGTGAG ATGCTGATGG GGATCTATGA ACGGATCCGT AAGCGAGAGC 
     3121 TAAAGACCAA TGAGGACCAT GTGTCCCAGG TGCAGAAGGT GGAGAAGCTC ATTGTGGGGA 
     3181 AAAAGCCGAT CGGATCCCTG CATCCCGGGC TCGGCTGTGT GCTCTCTCTG CCCCACCGTC 
     3241 GGTTGGTCTG CTACTGCCGG CTCTTTGAGG TTCCAGACCC AAACAAGCCC CAGAAACTCG 
     3301 GACTACACCA GCGAGAAATC TTCCTGTTCA ACGACCTCCT GGTGGTCACC AAGATCTTCC 
     3361 AGAAGAAGAA GAACTCGGTG ACGTACAGCT TCCGACAGTC CTTCTCCTTG TACGGCATGC 
     3421 AGGTCCTGCT CTTCGAGAAC CAGTACTACC CCAATGGCAT CCGGCTCACC TCGTCTGTCC 
     3481 CCGGAGCAGA TATCAAAGTG TTAATAAACT TCAACGCCCC CAACCCTCAA GACCGGAAGA 
     3541 AATTCACCGA TGACCTGCGG GAGTCCATTG CGGAAGTCCA AGAGATGGAG AAGCACAGGA 
     3601 TAGAGTCGGA GCTCGAGAAG CAGAAAGGCG TCGTGCGGCC CAGCATGTCC CAGTGCTCTA 
     3661 GCCTCAAAAA GGAGTCGGGC AACGGAACAC TGAGCCGGGC CTGCCTGGAC GACAGCTATG 
     3721 CCAGCGGTGA GGGCCTCAAG CGCAGCGCCC TCAGCAGCTC CCTGCGGGAC CTCTCGGAAG 
     3781 CCGGGAAGCG AGGGCGTCGC AGCAGTGCGG GATCGCTAGA GAGCAATGTG GAAGGGTCCA 
     3841 TCATTAGCAG TCCTCACATG CGCCGGAGAG CTACATCAAC ACGAGAGTGT CCATCTCGCC 
     3901 CACACCAGAC TATGCCCAAC TCATCTTCCC TCCTGGGCTC CTTATTCGGG AGCAAGAGAG 
     3961 GGAAGCCCCC TCCCCAGGCC CACCTGCCCT CAGCCCCAGC CCTGCCACCC CCCCACCCAC 
     4021 CGGTGGTCCT GCCTCACTTG CAGCACTCTG TGGCTGGCCA CCACCTGGGG CCCCCAGAGG 
     4081 GGCTGCCGCA GGCCGCCATG CACGGGCATC ACACCCAGTA CTGCCACATG CAGAACCCTC 
     4141 CCCCGTACCA CCATCACCAC CACCACCACC CACCCCAGCA CATCCAGCAC GCACACCAGT 
     4201 ACCACCACGG CCCCCATGGG GGCCACCCAG CCTACGGGGC CCATGCCCAC GGCCACCCGC 
     4261 CGCTGCCCTC GGCCCACGTG GGGCACACAG TGCACCACCA TGGGCAGCCC CCTGCCCCGC 
     4321 CGCCCCCCAC CAGCAGCAAG GCCAAACCCA GCGGCATCAG CACAATTGTG ACGCGTACGC 
     4381 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4441 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4501 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4561 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4621 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4681 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4741 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4801 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4861 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4921 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4981 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5041 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5101 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5161 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5221 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5281 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5341 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5401 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5461 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5521 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5581 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5641 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5701 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5761 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5821 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5881 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5941 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6001 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6061 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6121 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6181 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6241 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6301 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6361 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6421 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6481 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6541 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6601 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6661 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6721 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6781 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6841 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6901 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6961 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7021 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7081 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7141 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7201 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7261 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7321 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7381 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7441 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7501 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7621 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7681 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7741 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7801 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7861 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7921 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7981 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8041 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8101 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8161 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8221 TTT
//