LOCUS       RC226329                8205 bp    DNA     circular  SYN 
DEFINITION  ZEB1 (Myc-DDK-tagged)-Human zinc finger E-box binding homeobox 1 (ZEB1), transcript variant 1
ACCESSION   NM_001128128
VERSION     NM_001128128.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4362..4453 
                     /label="Myc-DDK"  
     misc_feature    4483..5068 
                     /label="PolyA signal"  
     misc_feature    4540..4559 
                     /label="Restriction Enzyme"  
     misc_feature    5344..6016 
                     /label="ColE1"  
     misc_feature    6446..7240 
                     /label="Kan/Neo"  
     misc_feature    7275..7577 
                     /label="SV40 ori"  
     misc_feature    7663..8140 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4344..4350 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4344 
                     /label="ZEB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTTACA AATTATAATA CTGTGGTAGA AACAAATTCA GATTCAGATG 
     1081 ATGAAGACAA ACTGCATATT GTGGAAGAAG AAAGTGTTAC AGATGCAGCT GACTGTGAAG 
     1141 GTGTACCAGA GGATGACCTG CCAACAGACC AGACAGTGTT ACCAGGGAGG AGCAGTGAAA 
     1201 GAGAAGGGAA TGCTAAGAAC TGCTGGGAGG ATGACACAGG AAAGGAAGGG CAAGAAATCC 
     1261 TGGGGCCTGA AGCTCAGGCA GATGAAGCAG GATGTACAGT AAAAGATGAT GAATGCGAGT 
     1321 CAGATGCAGA AAATGAGCAA AACCATGATC CTAATGTTGA AGAGTTTCTA CAACAACAAG 
     1381 ACACTGCTGT CATTTTTCCT GAGGCACCTG AAGAGGACCA GAGGCAGGGC ACACCAGAAG 
     1441 CCAGTGGTCA TGATGAAAAT GGAACACCAG ATGCATTTTC ACAATTACTC ACCTGTCCAT 
     1501 ATTGTGATAG AGGCTATAAA CGCTTTACCT CTCTGAAAGA ACACATTAAA TATCGTCATG 
     1561 AAAAGAATGA AGATAACTTT AGTTGCTCCC TGTGCAGTTA CACCTTTGCA TACAGAACCC 
     1621 AACTTGAACG TCACATGACA TCACATAAAT CAGGAAGAGA TCAAAGACAT GTGACGCAGT 
     1681 CTGGGTGTAA TCGTAAATTC AAATGCACTG AGTGTGGAAA AGCTTTCAAA TACAAACATC 
     1741 ACCTAAAAGA GCACTTAAGA ATTCACAGTG GAGAGAAGCC ATATGAATGC CCAAACTGCA 
     1801 AGAAACGCTT TTCCCATTCT GGCTCCTATA GCTCACACAT AAGCAGTAAG AAATGTATCA 
     1861 GCTTGATACC TGTGAATGGG CGACCAAGAA CAGGACTCAA GACATCTCAG TGTTCTTCAC 
     1921 CGTCTCTTTC AGCATCACCA GGCAGTCCCA CACGACCACA GATACGGCAA AAGATAGAGA 
     1981 ATAAACCCCT TCAAGAACAA CTTTCTGTTA ACCAAATTAA AACTGAACCT GTGGATTATG 
     2041 AATTCAAACC CATAGTGGTT GCTTCAGGAA TCAACTGTTC AACCCCTTTA CAAAATGGGG 
     2101 TTTTCACTGG TGGTGGCCCA TTACAGGCAA CCAGTTCTCC TCAGGGCATG GTGCAAGCTG 
     2161 TTGTTCTGCC AACAGTTGGT TTGGTGTCTC CCATAAGTAT CAATTTAAGT GATATTCAGA 
     2221 ATGTACTTAA AGTGGCGGTA GATGGTAATG TAATAAGGCA AGTGTTGGAG AATAATCAAG 
     2281 CCAATCTTGC ATCCAAAGAA CAAGAAACAA TCAATGCTTC ACCCATACAA CAAGGTGGCC 
     2341 ATTCTGTTAT TTCAGCCATC AGTCTTCCTT TGGTTGATCA AGATGGAACA ACCAAAATTA 
     2401 TCATCAACTA CAGTCTTGAG CAGCCTAGCC AACTTCAAGT TGTTCCTCAA AATTTAAAAA 
     2461 AAGAAAATCC AGTCGCTACA AACAGTTGTA AAAGTGAAAA GTTACCAGAA GATCTTACTG 
     2521 TTAAGTCTGA GAAGGACAAA AGCTTTGAAG GGGGGGTGAA TGATAGCACT TGTCTTCTGT 
     2581 GTGATGATTG TCCAGGAGAT ATTAATGCAC TTCCAGAATT AAAGCACTAT GACCTAAAGC 
     2641 AGCCTACTCA GCCTCCTCCA CTCCCTGCAG CAGAAGCTGA GAAGCCTGAG TCCTCTGTTT 
     2701 CATCAGCTAC TGGAGATGGC AATTTGTCTC CTAGTCAGCC ACCTTTAAAG AACCTCTTGT 
     2761 CTCTCCTAAA AGCATATTAT GCTTTGAATG CACAACCAAG TGCAGAAGAG CTCTCAAAAA 
     2821 TTGCTGATTC AGTAAACCTA CCACTGGATG TAGTAAAAAA GTGGTTTGAA AAGATGCAAG 
     2881 CTGGACAGAT TTCAGTGCAG TCTTCTGAAC CATCTTCTCC TGAACCAGGC AAAGTAAATA 
     2941 TCCCTGCCAA GAACAATGAT CAGCCTCAAT CTGCAAATGC AAATGAACCC CAGGACAGCA 
     3001 CAGTAAATCT ACAAAGTCCT TTGAAGATGA CTAACTCCCC AGTTTTACCA GTGGGATCAA 
     3061 CCACCAATGG TTCCAGAAGT AGTACACCAT CCCCATCACC TCTAAACCTT TCCTCATCCA 
     3121 GAAATACACA GGGTTACTTG TACACAGCTG AGGGTGCACA AGAAGAGCCA CAAGTAGAAC 
     3181 CTCTTGATCT TTCACTACCA AAGCAACAGG GAGAATTATT AGAAAGGTCA ACTATCACTA 
     3241 GTGTTTACCA GAACAGTGTT TATTCTGTCC AGGAAGAACC CTTGAACTTG TCTTGCGCAA 
     3301 AAAAGGAGCC ACAAAAGGAC AGTTGTGTTA CAGACTCAGA ACCAGTTGTA AATGTAATCC 
     3361 CACCAAGTGC CAACCCCATA AATATCGCTA TACCTACAGT CACTGCCCAG TTACCCACAA 
     3421 TCGTGGCCAT TGCTGACCAG AACAGTGTTC CATGCTTAAG AGCGCTAGCT GCCAATAAGC 
     3481 AAACGATTCT GATTCCCCAG GTGGCATACA CCTACTCAAC TACGGTCAGC CCTGCAGTCC 
     3541 AAGAACCACC CTTGAAAGTG ATCCAGCCAA ATGGAAATCA GGATGAAAGA CAAGATACTA 
     3601 GCTCAGAAGG AGTATCAAAT GTAGAGGATC AGAATGACTC TGATTCTACA CCGCCCAAAA 
     3661 AGAAAATGCG GAAGACAGAA AATGGAATGT ATGCTTGTGA TTTGTGTGAC AAGATATTCC 
     3721 AAAAGAGTAG TTCATTATTG AGACATAAAT ATGAACACAC AGGTAAAAGA CCTCATGAGT 
     3781 GTGGAATCTG TAAAAAGGCA TTTAAACACA AACATCATTT GATTGAACAC ATGCGATTAC 
     3841 ATTCTGGAGA AAAGCCCTAT CAATGTGACA AATGTGGAAA GCGCTTCTCA CACTCTGGGT 
     3901 CTTATTCTCA ACACATGAAT CATCGCTACT CCTACTGTAA GAGAGAAGCG GAAGAACGTG 
     3961 ACAGCACAGA GCAGGAAGAG GCAGGGCCTG AAATCCTCTC GAATGAGCAC GTGGGTGCCA 
     4021 GGGCGTCTCC CTCACAGGGC GACTCGGACG AGAGAGAGAG TTTGACAAGG GAAGAGGATG 
     4081 AAGACAGTGA AAAAGAGGAA GAGGAGGAGG ATAAAGAGAT GGAAGAATTG CAGGAAGAAA 
     4141 AAGAATGTGA AAAACCACAA GGGGATGAGG AAGAGGAGGA GGAGGAGGAA GAAGTGGAAG 
     4201 AAGAAGAGGT AGAAGAGGCA GAGAATGAGG GAGAAGAAGC AAAAACTGAA GGTCTGATGA 
     4261 AGGATGACAG GGCTGAAAGT CAAGCAAGCA GCTTAGGACA AAAAGTAGGC GAGAGTAGTG 
     4321 AGCAAGTGTC TGAAGAAAAG ACAAATGAAG CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4381 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4441 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6001 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6061 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6121 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6181 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6241 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6301 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6361 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6421 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6481 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6541 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6601 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6661 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6721 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6781 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6841 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6901 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6961 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7021 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7081 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7141 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7201 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7261 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7321 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7381 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7441 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7621 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7681 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7741 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7801 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7861 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7921 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7981 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8041 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8101 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8161 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//