LOCUS       RC226312                8049 bp    DNA     circular  SYN 
DEFINITION  LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 3
ACCESSION   NM_001112718
VERSION     NM_001112718.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4206..4297 
                     /label="Myc-DDK"  
     misc_feature    4327..4912 
                     /label="PolyA signal"  
     misc_feature    4384..4403 
                     /label="Restriction Enzyme"  
     misc_feature    5188..5860 
                     /label="ColE1"  
     misc_feature    6290..7084 
                     /label="Kan/Neo"  
     misc_feature    7119..7421 
                     /label="SV40 ori"  
     misc_feature    7507..7984 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4194 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="LIMCH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGTCCC GCTCTCGGTC TGGAAGCTCT TCAGCCCCTG CAGCCCGAGC 
     1081 CGCCCCCCGA GCCCGCCTTC TCCGAGGCGC AGAAGTGGAT TGAGCAAGTA ACTGGCAGAA 
     1141 GTTTTGGTGA TAAAGATTTT CGGACAGGTT TAGAAAATGG AATCCTCCTC TGCGAGTTGC 
     1201 TGAATGCTAT AAAGCCAGGA CTTGTTAAAA AGATCAATAG ATTGCCTACC CCCATTGCAG 
     1261 GACTGGACAA TATTATCTTA TTCTTGAGAG GTTGTAAAGA GCTCGGCCTT AAAGAATCTC 
     1321 AACTTTTTGA CCCGAGTGAC CTCCAGGATA CATCCAACAG AGTAACAGTC AAGAGCCTTG 
     1381 ATTATAGTAG GAAGCTGAAA AATGTATTAG TTACCATTTA CTGGCTGGGA AAAGCAGCAA 
     1441 ACAGCTGCAC ATCCTACAGC GGAACGACAC TAAACCTGAA GGAGTTTGAA GGATTGTTGG 
     1501 CTCAGATGCG AAAGGACACT GATGACATTG AAAGTCCTAA ACGCAGTATC CGAGACAGTG 
     1561 GCTACATCGA CTGCTGGGAT TCCGAGCGCA GCGACTCCCT CTCTCCTCCT CGCCACGGCA 
     1621 GAGATGATTC CTTCGACAGC CTGGATTCCT TTGGCTCTCG CTCTCGGCAG ACGCCTTCAC 
     1681 CAGATGTAGT CCTCAGGGGA AGCAGCGATG GGAGAGGAAG CGACTCTGAA TCCGACTTGC 
     1741 CTCATCGGAA GCTGCCAGAT GTGAAGAAGG ATGACATGTC TGCACGGCGG ACTTCCCATG 
     1801 GTGAGCCGAA ATCAGCAGTG CCTTTTAACC AGTACCTCCC GAACAAAAGC AATCAGACGG 
     1861 CCTACGTCCC CGCGCCTCTG AGAAAGAAGA AAGCAGAGAG AGAGGAATAC CGCAAGAGCT 
     1921 GGAGTACCGC CACCTCCCCG CTGGGTGGGG AGAGGCCCTT CAGATACGGT CCGAGAACTC 
     1981 CTGTGTCTGA TGACGCAGAG AGCACCAGCA TGTTTGACAT GCGGTGTGAG GAGGAGGCCG 
     2041 CGGTGCAGCC GCACAGCAGG GCCCGCCAGG AGCAGCTGCA GCTGATAAAT AACCAGCTGA 
     2101 GGGAAGAGGA CGACAAATGG CAAGATGACC TGGCTCGTTG GAAGAGTCGT AGAAGAAGTG 
     2161 TTTCTCAGGA CTTAATCAAG AAAGAGGAAG AAAGGAAAAA AATGGAGAAG TTACTGGCTG 
     2221 GAGAAGATGG GACAAGTGAA CGAAGGAAAA GCATCAAAAC CTACAGAGAA ATTGTTCAAG 
     2281 AAAAAGAGCG GAGAGAGAGA GAGCTGCATG AAGCATATAA GAACGCTCGG TCCCAGGAGG 
     2341 AGGCAGAGGG GATCCTTCAA CAGTACATTG AGAGGTTCAC CATCAGTGAG GCTGTTCTCG 
     2401 AACGCTTGGA GATGCCAAAA ATTCTGGAAA GAAGCCATTC AACAGAGCCA AATTTATCCT 
     2461 CCTTCCTGAA TGACCCCAAT CCCATGAAAT ACCTGCGGCA ACAGTCACTG CCTCCACCCA 
     2521 AATTCACTGC CACTGTTGAA ACCACCATTG CTCGTGCCAG TGTTCTGGAT ACCAGCATGT 
     2581 CAGCAGGCAG TGGGTCTCCA AGCAAAACTG TCACTCCCAA AGCAGTGCCT ATGCTGACAC 
     2641 CCAAGCCTTA CTCCCAGCCC AAAAATTCTC AAGATGTTCT GAAGACCTTT AAGGTAGACG 
     2701 GGAAAGTCAG TGTGAATGGA GAGACGGTTC ATAGAGAGGA GGAGAAGGAA AGAGAGTGTC 
     2761 CCACGGTGGC ACCTGCCCAC TCCTTAACCA AATCCCAGAT GTTTGAAGGT GTGGCCAGAG 
     2821 TGCACGGGTC TCCACTGGAG CTGAAACAAG ACAACGGTAG CATCGAGATC AACATAAAGA 
     2881 AGCCAAACTC TGTTCCCCAA GAGCTCGCAG CAACCACTGA GAAAACGGAA CCGAATAGTC 
     2941 AAGAGGACAA GAATGATGGT GGAAAATCAA GAAAAGGGAA TATAGAACTT GCCTCATCAG 
     3001 AACCACAGCA TTTTACAACA ACTGTGACTC GATGCAGCCC GACCGTGGCC TTTGTGGAAT 
     3061 TTCCCTCCAG CCCCCAGCTG AAGAATGATG TGTCGGAAGA AAAAGACCAG AAGAAACCAG 
     3121 AAAATGAAAT GAGTGGAAAG GTGGAGTTGG TGCTGTCACA AAAGGTGGTA AAGCCAAAAT 
     3181 CTCCAGAACC CGAAGCAACG CTGACATTTC CATTTCTGGA CAAAATGCCT GAAGCCAACC 
     3241 AACTACATTT GCCAAATCTC AATTCTCAAG TGGATTCTCC AAGCAGTGAG AAGTCACCTG 
     3301 TTATGACACC TTTTAAGTTC TGGGCATGGG ACCCAGAAGA GGAGCGCAGG CGACAGGAAA 
     3361 AATGGCAACA GGAACAGGAA CGTTTGCTCC AGGAGAGATA CCAGAAGGAG CAGGACAAGC 
     3421 TGAAAGAAGA GTGGGAAAAG GCCCAAAAGG AGGTGGAAGA GGAAGAACGC AGATACTATG 
     3481 AGGAGGAGCG TAAGATAATT GAAGACACTG TGGTTCCATT TACTGTTTCT TCAAGTTCCG 
     3541 CTGACCAGCT GTCTACCTCT TCCTCCATGA CTGAAGGCAG TGGGACAATG AATAAGATAG 
     3601 ACCTGGGAAA CTGTCAAGAT GAAAAACAAG ACAGAAGATG GAAGAAATCA TTCCAGGGAG 
     3661 ATGACAGTGA CTTATTGCTG AAGACTAGGG AAAGTGATCG ACTGGAGGAG AAGGGCAGCC 
     3721 TAACTGAAGG GGCCTTGGCT CATTCTGGGA ACCCTGTATC AAAAGGAGTC CATGAAGACC 
     3781 ATCAGCTGGA TACCGAGGCT GGGGCCCCAC ACTGTGGAAC AAACCCACAG CTTGCTCAGG 
     3841 ATCCATCCCA GAATCAGCAG ACATCAAATC CAACGCACAG TTCAGAAGAT GTGAAGCCAA 
     3901 AAACCCTCCC GCTGGATAAA AGCATTAACC ATCAGATCGA GTCTCCCAGT GAAAGGCGGA 
     3961 AGTCTATAAG TGGAAAGAAG CTGTGCTCTT CCTGTGGGCT TCCTTTGGGT AAAGGAGCTG 
     4021 CAATGATCAT CGAGACCCTC AATCTCTATT TTCACATCCA GTGTTTCAGG TGTGGAATTT 
     4081 GTAAAGGCCA GCTTGGAGAT GCAGTGAGTG GGACGGATGT TAGGATTCGA AATGGTCTCC 
     4141 TGAACTGTAA TGATTGCTAC ATGCGATCCA GAAGTGCCGG GCAGCCTACA ACATTGACGC 
     4201 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4261 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4321 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4381 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4441 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4501 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4561 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4621 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4681 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4741 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4801 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4861 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4921 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4981 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5041 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5101 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5161 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5221 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5281 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5341 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5401 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5461 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5521 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5581 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5641 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5701 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5761 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5821 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5881 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5941 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6001 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6061 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6121 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6181 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6241 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6301 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6361 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6421 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6481 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6541 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6601 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6661 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6721 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6781 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6841 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6901 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6961 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7021 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7081 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7141 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7201 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7261 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7321 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7501 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7561 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7621 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7681 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7741 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7801 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7861 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7921 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7981 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8041 GCGAATTTT
//