LOCUS       RC226310                8034 bp    DNA     circular  SYN 
DEFINITION  MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 3
ACCESSION   NM_001105078
VERSION     NM_001105078.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4191..4282 
                     /label="Myc-DDK"  
     misc_feature    4312..4897 
                     /label="PolyA signal"  
     misc_feature    4369..4388 
                     /label="Restriction Enzyme"  
     misc_feature    5173..5845 
                     /label="ColE1"  
     misc_feature    6275..7069 
                     /label="Kan/Neo"  
     misc_feature    7104..7406 
                     /label="SV40 ori"  
     misc_feature    7492..7969 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4173..4179 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4173 
                     /label="MECOM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGCGAA GACTATCCCC ATGAAACTAT GGCGCCGGAT ATCCACGAAG 
     1081 AACGGCAATA TCGCTGCGAA GACTGTGACC AGCTCTTTGA ATCTAAGGCT GAACTAGCAG 
     1141 ATCACCAAAA GTTTCCATGC AGTACTCCTC ACTCAGCATT TTCAATGGTT GAAGAGGACT 
     1201 TTCAGCAAAA ACTCGAAAGC GAGAATGATC TCCAAGAGAT ACACACGATC CAGGAGTGTA 
     1261 AGGAATGTGA CCAAGTTTTT CCTGATTTGC AAAGCCTGGA GAAACACATG CTGTCACATA 
     1321 CTGAAGAGAG GGAATACAAG TGTGATCAGT GTCCCAAGGC ATTTAACTGG AAGTCCAATT 
     1381 TAATTCGCCA CCAGATGTCA CATGACAGTG GAAAGCACTA TGAATGTGAA AACTGTGCCA 
     1441 AGGTTTTCAC GGACCCTAGC AACCTTCAGC GGCACATTCG CTCTCAGCAT GTCGGTGCCC 
     1501 GGGCCCATGC ATGCCCGGAG TGTGGCAAAA CGTTTGCCAC TTCGTCGGGC CTCAAACAAC 
     1561 ACAAGCACAT CCACAGCAGT GTGAAGCCCT TTATCTGTGA GGTCTGCCAT AAATCCTATA 
     1621 CTCAGTTTTC AAACCTTTGC CGTCATAAGC GCATGCATGC TGATTGCAGA ACCCAAATCA 
     1681 AGTGCAAAGA CTGTGGACAA ATGTTCAGCA CTACGTCTTC CTTAAATAAA CACAGGAGGT 
     1741 TTTGTGAGGG CAAGAACCAT TTTGCGGCAG GTGGATTTTT TGGCCAAGGC ATTTCACTTC 
     1801 CTGGAACCCC AGCTATGGAT AAAACGTCCA TGGTTAATAT GAGTCATGCC AACCCGGGCC 
     1861 TTGCTGACTA TTTTGGCGCC AATAGGCATC CTGCTGGTCT TACCTTTCCA ACAGCTCCTG 
     1921 GATTTTCTTT TAGCTTCCCT GGTCTGTTTC CTTCCGGCTT GTACCACAGG CCTCCTTTGA 
     1981 TACCTGCTAG TTCTCCTGTT AAAGGACTAT CAAGTACTGA ACAGACAAAC AAAAGTCAAA 
     2041 GTCCCCTCAT GACACATCCT CAGATACTGC CAGCTACACA GGATATTTTG AAGGCACTAT 
     2101 CTAAACACCC ATCTGTAGGG GACAATAAGC CAGTGGAGCT CCAGCCCGAG AGGTCCTCTG 
     2161 AAGAGAGGCC CTTTGAGAAA ATCAGTGACC AGTCAGAGAG TAGTGACCTT GATGATGTCA 
     2221 GTACACCAAG TGGCAGTGAC CTGGAAACAA CCTCGGGCTC TGATCTGGAA AGTGACATTG 
     2281 AAAGTGATAA AGAGAAATTT AAAGAAAATG GTAAAATGTT CAAAGACAAA GTAAGCCCTC 
     2341 TTCAGAATCT GGCTTCAATA AATAATAAGA AAGAATACAG CAATCATTCC ATTTTCTCAC 
     2401 CATCTTTAGA GGAGCAGACT GCGGTGTCAG GAGCTGTGAA TGATTCTATA AAGGCTATTG 
     2461 CTTCTATTGC TGAAAAATAC TTTGGTTCAA CAGGACTGGT GGGGCTGCAA GACAAAAAAG 
     2521 TTGGAGCTTT ACCTTACCCT TCCATGTTTC CCCTCCCATT TTTTCCAGCA TTCTCTCAAT 
     2581 CAATGTACCC ATTTCCTGAT AGAGACTTGA GATCGTTACC TTTGAAAATG GAACCCCAAT 
     2641 CACCAGGTGA AGTAAAGAAA CTGCAGAAGG GCAGCTCTGA GTCCCCCTTT GATCTCACCA 
     2701 CTAAGCGAAA GGATGAGAAG CCCTTGACTC CAGTCCCCTC CAAGCCTCCA GTGACACCTG 
     2761 CCACAAGCCA AGACCAGCCC CTGGATCTAA GTATGGGCAG TAGGAGTAGA GCCAGTGGGA 
     2821 CAAAGCTGAC TGAGCCTCGA AAAAACCACG TGTTTGGGGG AAAAAAAGGA AGCAACGTCG 
     2881 AATCAAGACC TGCTTCAGAT GGTTCCTTGC AGCATGCAAG ACCCACTCCT TTCTTTATGG 
     2941 ACCCTATTTA CAGAGTAGAG AAAAGAAAAC TAACTGACCC ACTTGAAGCT TTAAAAGAGA 
     3001 AATACTTGAG GCCTTCTCCA GGATTCTTGT TTCACCCACA ATTCCAACTG CCTGATCAGA 
     3061 GAACTTGGAT GTCAGCTATT GAAAACATGG CAGAAAAGCT AGAGAGCTTC AGTGCCCTGA 
     3121 AACCTGAGGC CAGTGAGCTC TTACAGTCAG TGCCCTCTAT GTTCAACTTC AGGGCGCCTC 
     3181 CCAATGCCCT GCCAGAGAAC CTTCTGCGGA AGGGAAAGGA GCGCTATACC TGCAGATACT 
     3241 GTGGCAAGAT TTTTCCAAGG TCTGCAAACC TAACACGGCA CTTGAGAACC CACACAGGAG 
     3301 AGCAGCCTTA CAGATGCAAA TACTGTGACA GATCATTTAG CATATCTTCT AACTTGCAAA 
     3361 GGCATGTTCG CAACATCCAC AATAAAGAGA AGCCATTTAA GTGTCACTTA TGTGATAGGT 
     3421 GTTTTGGTCA ACAAACCAAT TTAGACAGAC ACCTAAAGAA ACATGAGAAT GGGAACATGT 
     3481 CCGGTACAGC AACATCGTCG CCTCATTCTG AACTGGAAAG TACAGGTGCG ATTCTGGATG 
     3541 ACAAAGAAGA TGCTTACTTC ACAGAAATTC GAAATTTCAT TGGGAACAGC AACCATGGCA 
     3601 GCCAATCTCC CAGGAATGTG GAGGAGAGAA TGAATGGCAG TCATTTTAAA GATGAAAAGG 
     3661 CTTTGGTGAC CAGTCAAAAT TCAGACTTGC TGGATGATGA AGAAGTTGAA GATGAGGTGT 
     3721 TGTTAGATGA GGAGGATGAA GACAATGATA TTACTGGAAA AACAGGAAAG GAACCAGTGA 
     3781 CAAGTAATTT ACATGAAGGA AACCCTGAGG ATGACTATGA AGAAACCAGT GCCCTGGAGA 
     3841 TGAGTTGCAA GACATCCCCA GTGAGGTATA AAGAGGAAGA ATATAAAAGT GGACTTTCTG 
     3901 CTCTAGATCA TATAAGGCAC TTCACAGATA GCCTCAAAAT GAGGAAAATG GAAGATAATC 
     3961 AATATTCTGA AGCTGAGCTG TCTTCTTTTA GTACTTCCCA TGTGCCAGAG GAACTTAAGC 
     4021 AGCCGTTACA CAGAAAGTCC AAATCGCAGG CATATGCTAT GATGCTGTCA CTGTCTGACA 
     4081 AGGAGTCCCT CCATTCTACA TCCCACAGTT CTTCCAACGT GTGGCACAGT ATGGCCAGGG 
     4141 CTGCGGCGGA ATCCAGTGCT ATCCAGTCCA TAAGCCACGT AACGCGTACG CGGCCGCTCG 
     4201 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4261 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4321 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4381 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4441 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4501 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4561 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4621 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4681 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4741 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4801 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4861 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4921 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4981 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5041 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5101 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5161 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5221 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5281 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5341 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5401 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5461 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5521 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5581 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5641 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5701 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5761 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5821 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5881 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5941 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6001 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6061 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6121 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6181 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6241 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6301 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6361 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6421 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6481 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6541 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6601 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6661 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6721 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6781 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6841 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6901 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6961 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7021 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7081 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7141 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7201 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7261 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7381 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7441 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7501 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7561 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7621 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7681 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7741 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7801 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7861 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7921 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7981 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//