LOCUS       RC226309                8037 bp    DNA     circular  SYN 
DEFINITION  CPS1 (Myc-DDK-tagged)-Human carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant 3
ACCESSION   NM_001122634
VERSION     NM_001122634.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4192..4283 
                     /label="Myc-DDK"  
     misc_feature    4313..4898 
                     /label="PolyA signal"  
     misc_feature    4370..4389 
                     /label="Restriction Enzyme"  
     misc_feature    5174..5846 
                     /label="ColE1"  
     misc_feature    6276..7070 
                     /label="Kan/Neo"  
     misc_feature    7105..7407 
                     /label="SV40 ori"  
     misc_feature    7493..7970 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4167..4174 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4167 
                     /label="CPS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGAAGAA AATGTCAAAA CTGTTCTGAT GAACCCAAAC ATTGCATCAG 
     1081 TCCAGACCAA TGAGGTGGGC TTAAAGCAAG CGGATACTGT CTACTTTCTT CCCATCACCC 
     1141 CTCAGTTTGT CACAGAGGTC ATCAAGGCAG AACAGCCAGA TGGGTTAATT CTGGGCATGG 
     1201 GTGGCCAGAC AGCTCTGAAC TGTGGAGTGG AACTATTCAA GAGAGGTGTG CTCAAGGAAT 
     1261 ATGGTGTGAA AGTCCTGGGA ACTTCAGTTG AGTCCATTAT GGCTACGGAA GACAGGCAGC 
     1321 TGTTTTCAGA TAAACTAAAT GAGATCAATG AAAAGATTGC TCCAAGTTTT GCAGTGGAAT 
     1381 CGATTGAGGA TGCACTGAAG GCAGCAGACA CCATTGGCTA CCCAGTGATG ATCCGTTCCG 
     1441 CCTATGCACT GGGTGGGTTA GGCTCAGGCA TCTGTCCCAA CAGAGAGACT TTGATGGACC 
     1501 TCAGCACAAA GGCCTTTGCT ATGACCAACC AAATTCTGGT GGAGAAGTCA GTGACAGGTT 
     1561 GGAAAGAAAT AGAATATGAA GTGGTTCGAG ATGCTGATGA CAATTGTGTC ACTGTCTGTA 
     1621 ACATGGAAAA TGTTGATGCC ATGGGTGTTC ACACAGGTGA CTCAGTTGTT GTGGCTCCTG 
     1681 CCCAGACACT CTCCAATGCC GAGTTTCAGA TGTTGAGACG TACTTCAATC AATGTTGTTC 
     1741 GCCACTTGGG CATTGTGGGT GAATGCAACA TTCAGTTTGC CCTTCATCCT ACCTCAATGG 
     1801 AATACTGCAT CATTGAAGTG AATGCCAGAC TGTCCCGAAG CTCTGCTCTG GCCTCAAAAG 
     1861 CCACTGGCTA CCCATTGGCA TTCATTGCTG CAAAGATTGC CCTAGGAATC CCACTTCCAG 
     1921 AAATTAAGAA CGTCGTATCC GGGAAGACAT CAGCCTGTTT TGAACCTAGC CTGGATTACA 
     1981 TGGTCACCAA GATTCCCCGC TGGGATCTTG ACCGTTTTCA TGGAACATCT AGCCGAATTG 
     2041 GTAGCTCTAT GAAAAGTGTA GGAGAGGTCA TGGCTATTGG TCGTACCTTT GAGGAGAGTT 
     2101 TCCAGAAAGC TTTACGGATG TGCCACCCAT CTATAGAAGG TTTCACTCCC CGTCTCCCAA 
     2161 TGAACAAAGA ATGGCCATCT AATTTAGATC TTAGAAAAGA GTTGTCTGAA CCAAGCAGCA 
     2221 CGCGTATCTA TGCCATTGCC AAGGCCATTG ATGACAACAT GTCCCTTGAT GAGATTGAGA 
     2281 AGCTCACATA CATTGACAAG TGGTTTTTGT ATAAGATGCG TGATATTTTA AACATGGAAA 
     2341 AGACACTGAA AGGCCTCAAC AGTGAGTCCA TGACAGAAGA AACCCTGAAA AGGGCAAAGG 
     2401 AGATTGGGTT CTCAGATAAG CAGATTTCAA AATGCCTTGG GCTCACTGAG GCCCAGACAA 
     2461 GGGAGCTGAG GTTAAAGAAA AACATCCACC CTTGGGTTAA ACAGATTGAT ACACTGGCTG 
     2521 CAGAATACCC ATCAGTAACA AACTATCTCT ATGTTACCTA CAATGGTCAG GAGCATGATG 
     2581 TCAATTTTGA TGACCATGGA ATGATGGTGC TAGGCTGTGG TCCATATCAC ATTGGCAGCA 
     2641 GTGTGGAATT TGATTGGTGT GCTGTCTCTA GTATCCGCAC ACTGCGTCAA CTTGGCAAGA 
     2701 AGACGGTGGT GGTGAATTGC AATCCTGAGA CTGTGAGCAC AGACTTTGAT GAGTGTGACA 
     2761 AACTGTACTT TGAAGAGTTG TCCTTGGAGA GAATCCTAGA CATCTACCAT CAGGAGGCAT 
     2821 GTGGTGGCTG CATCATATCA GTTGGAGGCC AGATTCCAAA CAACCTGGCA GTTCCTCTAT 
     2881 ACAAGAATGG TGTCAAGATC ATGGGCACAA GCCCCCTGCA GATCGACAGG GCTGAGGATC 
     2941 GCTCCATCTT CTCAGCTGTC TTGGATGAGC TGAAGGTGGC TCAGGCACCT TGGAAAGCTG 
     3001 TTAATACTTT GAATGAAGCA CTGGAATTTG CAAAGTCTGT GGACTACCCC TGCTTGTTGA 
     3061 GGCCTTCCTA TGTTTTGAGT GGGTCTGCTA TGAATGTGGT ATTCTCTGAG GATGAGATGA 
     3121 AAAAATTCCT AGAAGAGGCG ACTAGAGTTT CTCAGGAGCA CCCAGTGGTG CTGACAAAAT 
     3181 TTGTTGAAGG GGCCCGAGAA GTAGAAATGG ACGCTGTTGG CAAAGATGGA AGGGTTATCT 
     3241 CTCATGCCAT CTCTGAACAT GTTGAAGATG CAGGTGTCCA CTCGGGAGAT GCCACTCTGA 
     3301 TGCTGCCCAC ACAAACCATC AGCCAAGGGG CCATTGAAAA GGTGAAGGAT GCTACCCGGA 
     3361 AGATTGCAAA GGCTTTTGCC ATCTCTGGTC CATTCAACGT CCAATTTCTT GTCAAAGGAA 
     3421 ATGATGTCTT GGTGATTGAG TGTAACTTGA GAGCTTCTCG ATCCTTCCCC TTTGTTTCCA 
     3481 AGACTCTTGG GGTTGACTTC ATTGATGTGG CCACCAAGGT GATGATTGGA GAGAATGTTG 
     3541 ATGAGAAACA TCTTCCAACA TTGGACCATC CCATAATTCC TGCTGACTAT GTTGCAATTA 
     3601 AGGCTCCCAT GTTTTCCTGG CCCCGGTTGA GGGATGCTGA CCCCATTCTG AGATGTGAGA 
     3661 TGGCTTCCAC TGGAGAGGTG GCTTGCTTTG GTGAAGGTAT TCATACAGCC TTCCTAAAGG 
     3721 CAATGCTTTC CACAGGATTT AAGATACCCC AGAAAGGCAT CCTGATAGGC ATCCAGCAAT 
     3781 CATTCCGGCC AAGATTCCTT GGTGTGGCTG AACAATTACA CAATGAAGGT TTCAAGCTGT 
     3841 TTGCCACGGA AGCCACATCA GACTGGCTCA ACGCCAACAA TGTCCCTGCC ACCCCAGTGG 
     3901 CATGGCCGTC TCAAGAAGGA CAGAATCCCA GCCTCTCTTC CATCAGAAAA TTGATTAGAG 
     3961 ATGGCAGCAT TGACCTAGTG ATTAACCTTC CCAACAACAA CACTAAATTT GTCCATGATA 
     4021 ATTATGTGAT TCGGAGGACA GCTGTTGATA GTGGAATCCC TCTCCTCACT AATTTTCAGG 
     4081 TGACCAAACT TTTTGCTGAA GCTGTGCAGA AATCTCGCAA GGTGGACTCC AAGAGTCTTT 
     4141 TCCACTACAG GCAGTACAGT GCTGGAAAAG CAGCAAGCGG ACCGACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//