LOCUS       RC226279                7764 bp    DNA     circular  SYN 
DEFINITION  ASAP2 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2
ACCESSION   NM_001135191
VERSION     NM_001135191.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4012 
                     /label="Myc-DDK"  
     misc_feature    4042..4627 
                     /label="PolyA signal"  
     misc_feature    4099..4118 
                     /label="Restriction Enzyme"  
     misc_feature    4903..5575 
                     /label="ColE1"  
     misc_feature    6005..6799 
                     /label="Kan/Neo"  
     misc_feature    6834..7136 
                     /label="SV40 ori"  
     misc_feature    7222..7699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="ASAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGACCAG ATCTCCGTGT CGGAATTCGT GGCCGAGACC CATGAGGACT 
     1081 ACAAGGCGCC CACGGCCTCC AGCTTCACCA CCCGCACGGC GCAGTGCCGG AACACTGTGG 
     1141 CGGCCATCGA GGAGGCTTTG GACGTGGACC GGATGGTTCT TTACAAAATG AAGAAATCCG 
     1201 TGAAAGCAAT CAACAGCTCT GGGCTGGCTC ACGTGGAAAA TGAAGAGCAG TACACCCAGG 
     1261 CTCTGGAGAA GTTTGGCGGC AACCGTGTAT GCAGAGATGA CCCAGATTTA GGAAGTGCGT 
     1321 TCCTGAAGTT CTCAGTGTTT ACAAAGGAGT TGACAGCACT TTTCAAAAAC CTGATTCAGA 
     1381 ATATGAACAA CATAATCTCC TTCCCTTTGG ACAGTTTGCT GAAGGGGGAC CTGAAAGGAG 
     1441 TGAAAGGGGA TCTGAAAAAG CCTTTTGATA AAGCTTGGAA GGACTATGAA ACAAAAATAA 
     1501 CCAAGATAGA AAAGGAGAAA AAGGAACACG CCAAGCTCCA TGGGATGATT CGGACTGAAA 
     1561 TAAGCGGAGC GGAAATTGCC GAAGAGATGG AAAAGGAGAG GCGCTTCTTC CAGCTACAGA 
     1621 TGTGCGAGTA TCTGCTGAAG GTCAACGAAA TCAAGATTAA AAAGGGAGTA GATTTACTTC 
     1681 AGAATCTGAT CAAATACTTT CATGCCCAAT GCAATTTTTT TCAGGATGGA CTCAAAGCCG 
     1741 TGGAAAGCCT CAAACCTTCC ATTGAAACGC TGTCTACGGA TCTTCACACG ATCAAACAGG 
     1801 CCCAGGATGA AGAAAGAAGG CAGTTGATAC AGCTTCGAGA TATTTTGAAA TCCGCATTGC 
     1861 AGGTTGAACA GAAAGAGGAC TCCCAAATTC GTCAGAGCAC AGCTTATAGC TTACATCAGC 
     1921 CTCAGGGAAA CAAGGAACAT GGGACCGAGC GGAACGGCAG CCTCTACAAG AAGAGTGACG 
     1981 GGATCCGAAA AGTGTGGCAG AAAAGGAAAT GTTCAGTTAA AAATGGTTTT CTGACCATAT 
     2041 CCCATGGTAC CGCTAACCGG CCTCCTGCAA AGCTCAACCT GCTAACCTGC CAGGTGAAGA 
     2101 CCAACCCTGA GGAGAAGAAG TGCTTTGACC TTATTTCACA TGACAGAACT TACCACTTTC 
     2161 AAGCTGAAGA TGAACAGGAA TGTCAAATAT GGATGTCTGT GCTGCAAAAT AGCAAAGAAG 
     2221 AAGCTTTAAA CAATGCATTT AAGGGGGATG ACAATACTGG AGAAAATAAC ATCGTCCAAG 
     2281 AACTGACAAA GGAGATCATC TCAGAAGTGC AGAGGATGAC GGGCAATGAC GTCTGCTGTG 
     2341 ACTGTGGGGC GCCAGATCCT ACATGGCTTT CCACCAACCT GGGCATCCTG ACCTGCATCG 
     2401 AGTGTTCCGG AATCCACCGA GAGCTGGGGG TTCATTATTC CAGGATGCAG TCCCTGACCT 
     2461 TAGATGTACT GGGAACATCT GAGCTGCTGC TCGCCAAGAA TATTGGGAAT GCAGGCTTTA 
     2521 ATGAGATCAT GGAATGTTGC CTACCAGCTG AGGACTCAGT CAAACCCAAC CCAGGCAGCG 
     2581 ACATGAATGC AAGAAAGGAC TACATCACAG CCAAGTACAT CGAGAGGAGA TACGCAAGGA 
     2641 AGAAGCACGC GGATAACGCG GCGAAGCTTC ACAGTCTTTG CGAGGCCGTC AAAACGAGAG 
     2701 ATATTTTTGG ATTGCTCCAA GCTTATGCTG ATGGTGTGGA TCTTACGGAA AAAATCCCAC 
     2761 TGGCCAACGG ACATGAGCCG GATGAAACGG CCCTCCACCT TGCAGTCAGA TCCGTGGATC 
     2821 GAACCTCTCT TCACATTGTA GACTTTTTAG TTCAGAACAG TGGGAACCTG GATAAACAGA 
     2881 CAGGGAAAGG CAGCACAGCC CTGCACTACT GCTGCCTGAC CGACAATGCC GAGTGCCTCA 
     2941 AGTTGCTCCT GCGGGGGAAG GCCTCCATCG AGATAGCAAA CGAGTCAGGA GAGACTCCGC 
     3001 TGGACATTGC CAAGCGCCTC AAGCACGAGC ACTGTGAGGA GCTGCTGACC CAAGCCTTAT 
     3061 CTGGAAGATT TAATTCTCAC GTTCACGTTG AATATGAATG GCGACTACTC CACGAAGACC 
     3121 TGGATGAAAG TGATGACGAC ATGGATGAGA AATTGCAGCC CAGTCCCAAC CGGCGGGAAG 
     3181 ACCGGCCCAT CAGCTTCTAC CAGCTGGGCT CCAACCAGCT TCAGTCTAAC GCTGTATCTT 
     3241 TGGCCAGAGA TGCTGCAAAC CTTGCCAAGG ACAAGCAGAG GGCTTTCATG CCCAGCATCT 
     3301 TGCAGAATGA GACTTACGGA GCCCTCCTGA GTGGCAGCCC ACCTCCCGCC CAGCCTGCAG 
     3361 CCCCCAGCAC CACCAGCGCC CCCCCGCTTC CTCCACGGAA TGTTGGCAAA GATCCCCTGA 
     3421 CCCCCACGCC GCCCCCACCC GTTGCCAAGA CGCCCAGCGT AATGGAAGCC TTGAGCCAGC 
     3481 CGAGCAAGCC TGCCCCGCCT GGGATCTCAC AGATCAGGCC CCCACCTCTG CCCCCACAGC 
     3541 CGCCCAGCCG CCTCCCGCAG AAGAAGCCTG CGCCGGGGGC TGACAAGTCC ACCCCACTGA 
     3601 CCAACAAAGG CCAACCGAGA GGACCTGTGG ATCTCTCTGC AACGGAAGCT CTGGGTCCTC 
     3661 TGTCCAATGC TATGGTCCTG CAGCCCCCTG CACCCATGCC TAGGAAGTCG CAGGCAACCA 
     3721 AGTTGAAGCC TAAGCGGGTG AAAGCGCTCT ATAACTGTGT GGCTGACAAC CCCGATGAGC 
     3781 TCACCTTCTC CGAGGGGGAT GTGATCATCG TGGACGGGGA GGAGGACCAG GAGTGGTGGA 
     3841 TTGGCCACAT TGATGGAGAT CCTGGTCGCA AAGGCGCATT CCCGGTGTCA TTTGTGCACT 
     3901 TTATCGCTGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3961 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4021 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4081 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4141 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4201 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4261 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4321 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4381 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4441 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4501 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4561 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4621 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4681 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5521 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5581 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5641 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5701 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5761 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5821 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5881 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5941 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6001 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6061 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6121 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6181 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6241 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6301 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6361 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6421 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6481 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6541 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6601 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6661 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6721 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6781 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6841 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6901 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6961 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7201 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7321 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7381 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7441 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7501 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7561 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7621 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7681 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7741 TAACAAAAAT TTAACGCGAA TTTT
//