LOCUS       RC226260                7623 bp    DNA     circular  SYN 
DEFINITION  USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 3
ACCESSION   NM_001110303
VERSION     NM_001110303.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3780..3871 
                     /label="Myc-DDK"  
     misc_feature    3901..4486 
                     /label="PolyA signal"  
     misc_feature    3958..3977 
                     /label="Restriction Enzyme"  
     misc_feature    4762..5434 
                     /label="ColE1"  
     misc_feature    5864..6658 
                     /label="Kan/Neo"  
     misc_feature    6693..6995 
                     /label="SV40 ori"  
     misc_feature    7081..7558 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3762..3768 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3762 
                     /label="USP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACTCC AGGGACCTTT GCCCTCACCT TGACTCCATA GGAGAGGTGA 
     1081 CCAAAGAGGA CTTGCTGCTC AAATCTAAGG GAACCTGTCA GTCGTGTGGG GTCACCGGAC 
     1141 CAAACCTATG GGCCTGTCTG CAGGTTGCCT GCCCCTATGT TGGCTGCGGA GAATCCTTTG 
     1201 CTGACCACAG CACCATTCAT GCACAGGCAA AAAAGCACAA CTTGACCGTG AACCTGACCA 
     1261 CGTTCCGACT GTGGTGTTAC GCCTGTGAGA AGGAGGTATT CCTGGAGCAG CGGCTGGCAG 
     1321 CCCCTCTGCT GGGCTCCTCT TCCAAGTTCT CTGAACAGGA CTCCCCGCCA CCCTCCCACC 
     1381 CTCTGAAAGC TGTTCCTATT GCTGTGGCTG ATGAAGGAGA GTCTGAGTCA GAGGACGATG 
     1441 ACCTGAAACC TCGAGGCCTC ACGGGCATGA AGAACCTCGG GAACTCCTGC TACATGAACG 
     1501 CTGCCCTGCA GGCCCTGTCC AATTGCCCGC CGCTGACTCA GTTCTTCTTG GAGTGTGGCG 
     1561 GCCTGGTGCG CACAGATAAG AAGCCAGCCC TGTGCAAGAG CTACCAGAAG CTGGTCTCTG 
     1621 AGGTCTGGCA TAAGAAACGG CCAAGCTACG TGGTCCCCAC CAGTCTGTCT CATGGGATCA 
     1681 AGTTGGTCAA CCCAATGTTC CGAGGCTATG CCCAGCAGGA CACCCAAGAG TTCCTTCGCT 
     1741 GCCTGATGGA CCAGCTGCAC GAGGAGCTCA AGGAGCCGGT GGTGGCCACG GTGGCGCTGA 
     1801 CGGAGGCTCG GGACTCAGAT TCGAGTGACA CGGATGAGAA ACGGGAGGGT GACCGGAGCC 
     1861 CATCAGAAGA TGAGTTCTTG TCCTGTGACT CGAGCAGTGA CCGGGGTGAG GGTGACGGGC 
     1921 AGGGGCGTGG CGGGGGCAGC TCGCAGGCCG AGACGGAGCT GCTGATCCCA GATGAGGCGG 
     1981 GCCGAGCCAT CTCTGAGAAG GAGCGGATGA AGGACCGCAA GTTCTCCTGG GGCCAGCAGC 
     2041 GTACAAACTC GGAGCAAGTG GACGAGGACG CTGATGTGGA CACTGCCATG GCTGCCCTTG 
     2101 ACGACCAGCC CGCGGAGGCC CAGCCCCCGT CACCACGGTC CTCCAGCCCC TGCCGGACGC 
     2161 CAGAGCCGGA CAATGATGCT CACCTACGCA GCTCCTCTCG CCCCTGCAGC CCCGTCCACC 
     2221 ACCACGAGGG CCATGCCAAG CTGTCTAGCA GCCCCCCTCG TGCAAGCCCC GTGAGGATGG 
     2281 CACCGTCGTA CGTGCTCAAG AAAGCCCAGG TATTGAGTGC TGGCAGCCGG AGGCGGAAGG 
     2341 AGCAGCGCTA CCGCAGCGTC ATCTCAGACA TCTTTGACGG CTCCATTCTC AGCCTTGTGC 
     2401 AGTGTCTCAC CTGTGACCGG GTATCCACCA CAGTGGAAAC GTTCCAGGAC TTATCACTGC 
     2461 CCATTCCTGG AAAGGAGGAC CTGGCCAAGC TCCATTCAGC CATCTACCAG AATGTGCCGG 
     2521 CCAAGCCAGG CGCCTGTGGG GACAGCTATG CCGCCCAGGG CTGGCTGGCC TTCATTGTGG 
     2581 AGTACATCCG ACGGTTTGTG GTATCCTGTA CCCCCAGCTG GTTTTGGGGG CCTGTCGTCA 
     2641 CCCTGGAAGA CTGCCTTGCT GCCTTCTTTG CCGCTGATGA GTTAAAGGGT GACAACATGT 
     2701 ACAGCTGTGA GCGGTGTAAG AAGCTGCGGA ACGGAGTGAA GTACTGCAAA GTCCTGCGGT 
     2761 TGCCCGAGAT CCTGTGCATT CACCTAAAGC GCTTTCGGCA CGAGGTGATG TACTCATTCA 
     2821 AGATCAACAG CCACGTCTCC TTCCCCCTCG AGGGGCTCGA CCTGCGCCCC TTCCTTGCCA 
     2881 AGGAGTGCAC ATCCCAGATC ACCACCTACG ACCTCCTCTC GGTCATCTGC CACCACGGCA 
     2941 CGGCAGGCAG TGGGCACTAC ATCGCCTACT GCCAGAACGT GATCAATGGG CAGTGGTACG 
     3001 AGTTTGATGA CCAGTACGTC ACAGAAGTCC ACGAGACGGT GGTGCAGAAC GCCGAGGGCT 
     3061 ACGTACTCTT CTACAGGAAG AGCAGCGAGG AGGCCATGCG GGAGCGACAG CAGGTGGTGT 
     3121 CCCTGGCCGC CATGCGGGAG CCCAGCCTGC TGCGGTTCTA CGTGTCCCGC GAGTGGCTCA 
     3181 ACAAGTTCAA CACCTTCGCG GAGCCAGGCC CCATCACCAA CCAGACCTTC CTCTGCTCCC 
     3241 ACGGAGGCAT CCCGCCCCAC AAATACCACT ACATCGACGA CCTGGTGGTC ATCCTGCCCC 
     3301 AGAACGTCTG GGAGCACCTG TACAACAGAT TCGGGGGTGG CCCCGCCGTG AACCACCTGT 
     3361 ACGTGTGCTC CATCTGCCAG GTGGAGATCG AGGCACTGGC CAAGCGCAGG AGGATCGAGA 
     3421 TCGACACCTT CATCAAGTTG AACAAGGCCT TCCAGGCCGA GGAGTCGCCG GGCGTCATCT 
     3481 ACTGCATCAG CATGCAGTGG TTCCGGGAGT GGGAGGCGTT CGTCAAGGGG AAGGACAACG 
     3541 AGCCCCCCGG GCCCATTGAC AACAGCAGGA TTGCACAGGT CAAAGGAAGC GGCCATGTCC 
     3601 AGCTGAAGCA GGGAGCTGAC TACGGGCAGA TTTCGGAGGA GACCTGGACC TACCTGAACA 
     3661 GCCTGTATGG AGGTGGCCCC GAGATTGCCA TCCGCCAGAG TGTGGCGCAG CCGCTGGGCC 
     3721 CAGAGAACCT GCACGGGGAG CAGAAGATCG AAGCCGAGAC GCGGGCCGTG ACGCGTACGC 
     3781 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3841 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3901 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3961 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4021 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4081 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4141 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4201 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4261 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4321 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4381 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4441 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4501 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4561 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4621 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4681 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4741 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4801 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4861 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4921 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4981 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5041 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5101 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5161 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5221 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5281 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5341 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5401 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5461 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5521 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5581 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5641 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5701 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5761 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5821 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5881 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5941 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6001 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6061 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6121 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6181 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6241 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6301 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6361 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6421 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6481 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6541 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6601 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6661 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6721 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6781 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6841 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6901 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6961 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7021 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7081 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7141 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7201 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7261 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7321 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7381 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7441 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7501 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7561 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7621 TTT
//