LOCUS       RC226250                7584 bp    DNA     circular  SYN 
DEFINITION  SLFN11 (Myc-DDK-tagged)-Human schlafen family member 11 (SLFN11), transcript variant 4
ACCESSION   NM_001104590
VERSION     NM_001104590.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3741..3832 
                     /label="Myc-DDK"  
     misc_feature    3862..4447 
                     /label="PolyA signal"  
     misc_feature    3919..3938 
                     /label="Restriction Enzyme"  
     misc_feature    4723..5395 
                     /label="ColE1"  
     misc_feature    5825..6619 
                     /label="Kan/Neo"  
     misc_feature    6654..6956 
                     /label="SV40 ori"  
     misc_feature    7042..7519 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3723..3729 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3723 
                     /label="SLFN11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAAAT CAGTGCCCCC TGGTTGTGGA ACCATCTTAC CCAGACCTGG 
     1081 TCATCAATGT AGGAGAAGTG ACTCTTGGAG AAGAAAACAG AAAAAAGCTG CAGAAAATTC 
     1141 AGAGAGACCA AGAGAAGGAG AGAGTTATGC GGGCTGCATG TGCTTTATTA AACTCAGGAG 
     1201 GAGGAGTGAT TCGAATGGCC AAGAAGGTTG AGCATCCCGT GGAGATGGGA CTGGATTTAG 
     1261 AACAGTCTTT GAGAGAGCTT ATTCAGTCTT CAGATCTGCA GGCTTTCTTT GAGACCAAGC 
     1321 AACAAGGAAG GTGTTTTTAC ATTTTTGTTA AATCTTGGAG CAGTGGCCCT TTCCCTGAAG 
     1381 ATCGCTCTGT CAAGCCCCGC CTTTGCAGCC TCAGTTCTTC ATTATACCGT AGATCTGAGA 
     1441 CCTCTGTGCG TTCCATGGAC TCAAGAGAGG CATTCTGTTT CCTGAAGACC AAAAGGAAGC 
     1501 CAAAAATCTT GGAAGAAGGA CCTTTTCACA AAATTCACAA GGGTGTATAC CAAGAGCTCC 
     1561 CTAACTCGGA TCCTGCTGAC CCAAACTCGG ATCCTGCTGA CCTAATTTTC CAAAAAGACT 
     1621 ATCTTGAATA TGGTGAAATC CTGCCTTTTC CTGAGTCTCA GTTAGTAGAG TTTAAACAGT 
     1681 TCTCTACAAA ACACTTCCAA GAATATGTAA AAAGGACAAT TCCAGAATAC GTCCCTGCAT 
     1741 TTGCAAACAC TGGAGGAGGC TATCTTTTTA TTGGAGTGGA TGATAAGAGT AGGGAAGTCC 
     1801 TGGGATGTGC AAAAGAAAAT GTTGACCCTG ACTCTTTGAG AAGGAAAATA GAACAAGCCA 
     1861 TATACAAACT ACCTTGTGTT CATTTTTGCC AACCCCAACG CCCGATAACC TTCACACTCA 
     1921 AAATTGTGGA TGTGTTAAAA AGGGGAGAGC TCTATGGCTA TGCTTGCATG ATCAGAGTAA 
     1981 ATCCCTTCTG CTGTGCAGTG TTCTCAGAAG CTCCCAATTC ATGGATAGTG GAGGACAAGT 
     2041 ACGTCTGCAG CCTGACAACC GAGAAATGGG TAGGCATGAT GACAGACACA GATCCAGATC 
     2101 TTCTACAGTT GTCTGAAGAT TTTGAATGTC AGCTGAGTCT ATCTAGTGGG CCTCCCCTTA 
     2161 GCAGACCAGT GTACTCCAAG AAAGGCCTGG AACATAAAAA GGAACTCCAG CAACTTTTAT 
     2221 TTTCAGTCCC ACCAGGATAT TTGCGATATA CTCCAGAGTC ACTCTGGAGG GACCTGATCT 
     2281 CAGAGCACAG AGGACTAGAG GAGTTAATAA ATAAGCAAAT GCAACCTTTC TTTCGGGGAA 
     2341 TTTTGATCTT CTCTAGAAGT TGGGCTGTGG ACCTGAACTT GCAGGAGAAG CCAGGAGTCA 
     2401 TCTGTGATGC TCTGCTGATA GCACAGAACA GCACCCCCAT TCTCTACACC ATTCTCAGGG 
     2461 AGCAGGATGC AGAGGGCCAG GACTACTGCA CTCGCACTGC CTTTACTTTG AAGCAGAAGC 
     2521 TAGTGAACAT GGGGGGCTAC ACCGGGAAGG TGTGTGTCAG GGCCAAGGTC CTCTGCCTGA 
     2581 GTCCTGAGAG CAGCGCAGAG GCCTTGGAGG CTGCAGTGTC TCCGATGGAT TACCCTGCGT 
     2641 CCTATAGCCT TGCAGGCACC CAGCACATGG AAGCCCTGCT GCAGTCCCTC GTGATTGTCT 
     2701 TACTCGGCTT CAGGTCTCTC TTGAGTGACC AGCTCGGCTG TGAGGTTTTA AATCTGCTCA 
     2761 CAGCCCAGCA GTATGAGATA TTCTCCAGAA GCCTCCGCAA GAACAGAGAG TTGTTTGTCC 
     2821 ACGGCTTACC TGGCTCAGGG AAGACCATCA TGGCCATGAA GATCATGGAG AAGATCAGGA 
     2881 ATGTGTTTCA CTGTGAGGCA CACAGAATTC TCTACGTTTG TGAAAACCAG CCTCTGAGGA 
     2941 ACTTTATCAG TGATAGAAAT ATCTGCCGAG CAGAGACCCG GAAAACTTTC CTAAGAGAAA 
     3001 ACTTTGAACA CATTCAACAC ATCGTCATTG ACGAAGCTCA GAATTTCCGT ACTGAAGATG 
     3061 GGGACTGGTA TGGGAAGGCA AAAAGCATCA CTCGGAGAGC AAAGGGTGGC CCAGGAATTC 
     3121 TCTGGATCTT TCTGGATTAC TTTCAGACCA GCCACTTGGA TTGCAGTGGC CTCCCTCCTC 
     3181 TCTCAGACCA ATATCCAAGA GAAGAGCTCA CCAGAATAGT TCGCAATGCA GATCCAATAG 
     3241 CCAAGTACTT ACAAAAAGAA ATGCAAGTAA TTAGAAGTAA TCCTTCATTT AACATCCCCA 
     3301 CTGGGTGCCT CGAGGTATTT CCTGAAGCCG AATGGTCCCA GGGTGTTCAG GGAACCTTAC 
     3361 GAATTAAGAA ATACTTGACT GTGGAGCAAA TAATGACCTG TGTGGCAGAC ACGTGCAGGC 
     3421 GCTTCTTTGA TAGGGGCTAT TCTCCAAAGG ATGTTGCTGT GCTTGTCAGC ACCGCAAAAG 
     3481 AAGTGGAGCA CTATAAGTAT GAGCTCTTGA AAGCAATGAG GAAGAAAAGG GTGGTGCAGC 
     3541 TCAGTGATGC ATGTGATATG TTGGGTGATC ACATTGTGTT GGACAGTGTT CGGCGATTCT 
     3601 CAGGCCTGGA AAGGAGCATA GTGTTTGGGA TCCATCCAAG GACAGCTGAC CCAGCTATCT 
     3661 TACCCAATGT TCTGATCTGT CTGGCTTCCA GGGCAAAACA ACACCTGTAT ATTTTTCCGT 
     3721 GGGGTGGCCA TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3781 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3841 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3901 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3961 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4021 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4081 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4141 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4201 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4261 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4321 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4381 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4441 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4501 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4561 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4621 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4681 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4741 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4801 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4861 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4921 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4981 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5041 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5101 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5161 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5221 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5281 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5341 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5401 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5461 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5521 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5581 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5641 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5701 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5761 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5821 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5881 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5941 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6001 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6061 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6121 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6181 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6241 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6301 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6361 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6421 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6481 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6541 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6601 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6661 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6721 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6781 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6841 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6901 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6961 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7021 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7081 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7141 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7201 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7261 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7321 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7381 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7441 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7501 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7561 TAACAAAAAT TTAACGCGAA TTTT
//