LOCUS       RC226203                7392 bp    DNA     circular  SYN 
DEFINITION  FSTL5 (Myc-DDK-tagged)-Human follistatin-like 5 (FSTL5), transcript variant 3
ACCESSION   NM_001128428
VERSION     NM_001128428.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3549..3640 
                     /label="Myc-DDK"  
     misc_feature    3670..4255 
                     /label="PolyA signal"  
     misc_feature    3727..3746 
                     /label="Restriction Enzyme"  
     misc_feature    4531..5203 
                     /label="ColE1"  
     misc_feature    5633..6427 
                     /label="Kan/Neo"  
     misc_feature    6462..6764 
                     /label="SV40 ori"  
     misc_feature    6850..7327 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3531..3537 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3531 
                     /label="FSTL5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTAAGTGC TGGTCAGTTG TCTTGGTTCT CGGATTCATT TTTCTGGAGT 
     1081 CGGAAGGAAG GCCAACCAAA GAAGGAGGAT ATGGCCTTAA ATCCTATCAG CCTCTAATGA 
     1141 GATTGCGACA TAAGGAAAAA AATCAAGAAA GTTCAAGAGT CAAAGGATTT ATGATTCAGG 
     1201 ATGGCCCTTT TGGATCTTGT GAAAATAAGT ACTGTGGTTT GGGAAGACAC TGTGTTACCA 
     1261 GCAGAGAGAC AGGGCAAGCA GAATGTGCCT GTATGGACCT TTGCAAACGT CACTACAAAC 
     1321 CTGTGTGTGG ATCTGACGGA GAATTCTATG AAAACCACTG TGAAGTGCAC AGAGCTGCTT 
     1381 GCCTGAAAAA ACAAAAGATT ACCATTGTTC ACAATGAAGA CTGCTTCTTT AAAGGAGATA 
     1441 AGTGCAAGAC TACTGAATAC AGCAAGATGA AAAATATGCT ATTAGATTTA CAAAATCAAA 
     1501 AATATATTAT GCAAGAAAAT GAAAATCCTA ATGGCGACGA CATATCTCGG AAGAAGCTAT 
     1561 TGGTGGATCA AATGTTTAAA TATTTTGATG CAGACAGTAA TGGACTTGTA GATATTAATG 
     1621 AACTAACTCA GGTGATAAAA CAGGAAGAAC TTGGCAAGGA TCTCTTTGAT TGTACTTTGT 
     1681 ATGTTCTATT GAAATATGAT GATTTTAATG CTGACAAGCA CCTGGCTCTT GAAGAATTTT 
     1741 ATAGAGCATT CCAAGTGATC CAGTTGAGTC TGCCAGAAGA TCAGAAACTA AGCATCACTG 
     1801 CAGCAACTGT GGGACAAAGT GCTGTTCTGA GCTGTGCCAT TCAAGGAACC CTGAGACCTC 
     1861 CCATTATCTG GAAAAGGAAC AATATTATTC TAAATAATTT AGATTTGGAA GACATCAATG 
     1921 ACTTTGGAGA TGATGGGTCC TTGTATATTA CTAAGGTTAC CACAACTCAC GTTGGCAATT 
     1981 ACACCTGCTA TGCAGATGGC TATGAACAAG TCTATCAGAC TCACATCTTC CAAGTGAATG 
     2041 TTCCTCCAGT CATCCGGGTG TATCCAGAGA GTCAGGCTAG AGAGCCTGGG GTAACTGCCA 
     2101 GTCTTAGGTG CCATGCAGAG GGCATACCAA AGCCTCAGCT TGGCTGGTTG AAGAATGGAA 
     2161 TTGATATTAC ACCAAAGCTT TCCAAACAAC TCACGCTTCA AGCAAATGGC AGTGAGGTTC 
     2221 ACATAAGCAA TGTGCGCTAT GAAGATACTG GAGCATACAC TTGTATCGCA AAGAATGAAG 
     2281 CAGGAGTGGA TGAAGACATC TCTTCTCTTT TTGTGGAAGA CTCTGCTAGA AAGACCCGTC 
     2341 TGGGAATTGG GAACATGTTC TATGTTTTTT ATGAAGATGG AATCAAAGTG ATACAACCCA 
     2401 TAGAATGTGA ATTTCAGAGG CACATTAAGC CTAGTGAAAA GCTCCTTGGA TTTCAGGATG 
     2461 AAGTCTGTCC CAAAGCTGAG GGAGATGAAG TTCAGAGGTG TGTGTGGGCA TCAGCTGTTA 
     2521 ATGTCAAAGA CAAGTTCATT TATGTTGCAC AGCCAACTTT GGACAGAGTC CTTATTGTTG 
     2581 ATGTGCAGTC CCAAAAAGTT GTTCAGGCAG TGAGCACAGA CCCTGTCCCA GTTAAATTAC 
     2641 ACTATGACAA ATCACATGAT CAGGTCTGGG TGCTAAGCTG GGGTACCTTG GAGAAGACAT 
     2701 CACCAACACT ACAGGTAATT ACCCTGGCCA GTGGGAATGT GCCTCACCAC ACGATCCACA 
     2761 CCCAACCAGT GGGAAAGCAA TTTGACAGAG TGGATGATTT TTTCATTCCC ACCACAACAC 
     2821 TCATTATCAC CCATATGAGG TTTGGATTTA TTCTTCATAA AGATGAAGCT GCACTACAAA 
     2881 AAATTGATCT TGAAACCATG TCATACATCA AGACAATTAA CTTGAAGGAC TATAAGTGCG 
     2941 TTCCTCAGTC ATTGGCATAT ACACACTTGG GAGGCTACTA CTTCATTGGC TGCAAACCTG 
     3001 ACAGCACCGG AGCAGTTTCC CCACAGGTCA TGGTGGACGG TGTAACTGAC TCAGTCATTG 
     3061 GGTTCAATAG TGATGTGACG GGCACTCCAT ATGTCTCTCC AGATGGCCAC TACCTTGTCA 
     3121 GCATTAATGA TGTGAAAGGT CTTGTAAGGG TTCAGTACAT TACCATCAGA GGAGAAATAC 
     3181 AGGAGGCTTT TGATATTTAC ACAAATCTGC ACATATCTGA TCTGGCATTT CAACCATCCT 
     3241 TTACTGAAGC CCACCAATAT AACATCTACG GTAGTTCAAG CACACAAACT GATGTGCTCT 
     3301 TTGTGGAGCT CTCTTCTGGG AAGGTCAAGA TGATAAAGAG TCTCAAGGAA CCACTCAAGG 
     3361 CAGAAGAATG GCCTTGGAAC CGGAAAAACA GGCAAATCCA GGACAGTGGC TTGTTTGGTC 
     3421 AATACCTGAT GACACCTTCC AAGGACTCTC TCTTCATCCT AGATGGACGA CTCAATAAAT 
     3481 TAAACTGTGA GATCACTGAA GTTGAAAAAG GAAATACAGT CATTTGGGTT GGAGATGCCA 
     3541 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3601 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3661 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3721 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3781 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3841 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3901 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3961 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4021 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4081 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4141 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4201 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4261 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4321 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4381 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4441 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4501 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4561 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4621 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4681 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4741 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4801 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4861 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4921 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4981 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5041 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5101 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5161 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5221 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5281 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5341 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5401 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5461 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5521 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5581 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5641 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5701 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5761 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5821 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5881 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5941 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6001 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6061 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6121 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6181 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6241 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6301 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6361 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6421 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6481 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6541 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6601 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6661 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6721 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6781 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6841 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6901 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6961 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7021 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7081 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7141 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7201 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7261 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7321 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7381 AACGCGAATT TT
//