LOCUS       RC226198L1                8865 bp    DNA     circular  SYN 
DEFINITION  CDC27 (Myc-DDK-tagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1
ACCESSION   NM_001114091
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7156..7336 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8064..8723 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7495..8063 
                     /label="ColE1"  
     misc_feature    6031..6051 
                     /label="LR50 primer"  
     misc_feature    5828..5899 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6040..6631 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5810..5816 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5810 
                     /label="CDC27"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GACGGTGCTG CAGGAACCCG TCCAGGCTGC 
     3361 TATATGGCAA GCACTAAACC ACTATGCTTA CCGAGATGCG GTTTTCCTCG CAGAACGCCT 
     3421 TTATGCAGAA GTACACTCAG AAGAAGCCTT GTTTTTACTG GCAACCTGTT ATTACCGCTC 
     3481 AGGAAAGGCA TATAAAGCAT ATAGACTCTT GAAAGGACAC AGTTGTACTA CACCGCAATG 
     3541 CAAATACCTG CTTGCAAAAT GTTGTGTTGA TCTCAGCAAG CTTGCAGAAG GGGAACAAAT 
     3601 CTTATCTGGT GGAGTGTTTA ATAAGCAGAA AAGCCATGAT GATATTGTTA CTGAGTTTGG 
     3661 TGATTCAGCT TGCTTTACTC TTTCATTGTT GGGACATGTA TATTGCAAGA CAGATCGGCT 
     3721 TGCCAAAGGA TCAGAATGTT ACCAAAAGAG CCTTAGTTTA AATCCTTTCC TCTGGTCTCC 
     3781 CTTTGAATCA TTATGTGAAA TAGGTGAAAA GCCAGATCCT GACCAAACAT TTAAATTCAC 
     3841 ATCTTTACAG AACTTTAGCA ACTGTCTGCC CAACTCTTGC ACAACACAAG TACCTAATCA 
     3901 TAGTTTATCT CACAGACAGC CTGAGACAGT TCTTACGGAA ACACCCCAGG ACACAATTGA 
     3961 ATTAAACAGA TTGAATTTAG AATCTTCCAA TTCAAAGTAC TCCTTGAATA CAGATTCCTC 
     4021 AGTGTCTTAT ATTGATTCAG CTGTAATTTC ACCTGATACT GTCCCACTGG GAACAGGAAC 
     4081 TTCCATATTA TCTAAACAGG TTCAAAATAA ACCAAAAACT GGTCGAAGTT TATTAGGAGG 
     4141 ACCAGCAGCT CTTAGTCCAT TAACCCCAAG TTTTGGGATT TTGCCATTAG AAACCCCAAG 
     4201 TCCTGGAGAT GGATCCTATT TACAAAACTA CACTAATACA CCTCCTGTAA TTGATGTGCC 
     4261 ATCCACCGGA GCCCCTTCAA AAAAGACTTT TCGTGTTTTA CAGTCTGTTG CCAGAATCGG 
     4321 CCAAACTGGA ACAAAGTCTG TCTTCTCACA GAGTGGAAAT AGCCGAGAGG TAACTCCAAT 
     4381 TCTTGCACAA ACACAAAGTT CTGGTCCACA AACAAGTACA ACACCTCAGG TATTGAGCCC 
     4441 CACTATTACA TCTCCCCCAA ACGCACTGCC TCGAAGAAGT TCACGACTCT TTACTAGTGA 
     4501 CAGCTCCACA ACCAAGGAGA ATAGCAAAAA ATTAAAAATG AAGTTTCCAC CTAAAATCCC 
     4561 AAACAGAAAA ACAAAAAGTA AAACTAATAA AGGAGGAATA ACTCAACCTA ACATAAATGA 
     4621 TAGCCTGGAA ATTACAAAAT TGGACTCTTC CATCATTTCA GAAGGGAAAA TATCCACAAT 
     4681 CACACCTCAG ATTCAGGCCT TTAATCTACA AAAAGCAGCA GCAGAAGGTT TGATGAGCCT 
     4741 TCTTCGTGAA ATGGGGAAAG GTTATTTAGC TTTGTGTTCA TACAACTGCA AAGAAGCTAT 
     4801 AAATATTTTG AGCCATCTAC CTTCTCACCA CTACAATACT GGTTGGGTAC TGTGCCAAAT 
     4861 TGGAAGGGCC TATTTTGAAC TTTCAGAGTA CATGCAAGCT GAAAGAATAT TCTCAGAGGT 
     4921 TAGAAGGATT GAGAATTATA GAGTTGAAGG CATGGAGATC TACTCTACAA CACTTTGGCA 
     4981 TCTTCAAAAA GATGTTGCTC TTTCAGTTCT GTCAAAAGAC TTAACAGACA TGGATAAAAA 
     5041 TTCGCCAGAG GCCTGGTGTG CTGCAGGGAA CTGTTTCAGT CTGCAACGGG AACATGATAT 
     5101 TGCAATTAAA TTCTTCCAGA GAGCTATCCA AGTTGATCCA AATTACGCTT ATGCCTATAC 
     5161 TCTATTAGGG CATGAGTTTG TCTTAACTGA AGAATTGGAC AAAGCATTAG CTTGTTTTCG 
     5221 AAATGCTATC AGAGTCAATC CTAGACATTA TAATGCATGG TATGGTTTAG GAATGATTTA 
     5281 TTACAAGCAA GAAAAATTCA GCCTTGCAGA AATGCATTTC CAAAAAGCGC TTGATATCAA 
     5341 CCCTCAAAGT TCAGTTTTAC TTTGCCACAT TGGAGTAGTT CAACATGCAC TGAAAAAATC 
     5401 AGAGAAGGCT TTGGATACCC TAAACAAAGC CATTGTCATT GATCCCAAGA ACCCTCTATG 
     5461 CAAATTTCAC AGAGCCTCAG TTTTATTTGC AAATGAAAAA TATAAGTCTG CTTTACAAGA 
     5521 ACTTGAAGAA TTGAAACAAA TTGTTCCCAA AGAATCCCTC GTTTACTTCT TAATAGGAAA 
     5581 GGTTTACAAG AAGTTAGGTC AAACGCACCT CGCCCTGATG AATTTCTCTT GGGCTATGGA 
     5641 TTTAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC GTTATCTTCC 
     5701 AGATGATGAG GAGCCAATAA CCCAAGAAGA ACAGATCATG GGAACAGATG AATCCCAGGA 
     5761 GAGCAGCATG ACAGATGCGG ATGACACACA ACTTCATGCA GCTGAAAGTG ATGAATTTAC 
     5821 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     5881 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     5941 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6001 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6061 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6121 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6181 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6241 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6301 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6361 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     6421 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     6481 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     6541 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     6601 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     6661 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     6721 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     6781 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     6841 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     6901 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     6961 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7021 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7081 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7141 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7201 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7261 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7321 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7381 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     7441 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     7501 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     7561 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     7621 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     7681 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     7741 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     7801 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     7861 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     7921 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7981 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8041 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8101 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8161 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8221 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8281 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8341 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     8401 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     8461 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     8521 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     8581 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     8641 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     8701 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     8761 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8821 CAAATAGGGG TCCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//