LOCUS       RC226163                7224 bp    DNA     circular  SYN 
DEFINITION  CLCN7 (Myc-DDK-tagged)-Human chloride channel 7 (CLCN7), transcript variant 2
ACCESSION   NM_001114331
VERSION     NM_001114331.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3381..3472 
                     /label="Myc-DDK"  
     misc_feature    3502..4087 
                     /label="PolyA signal"  
     misc_feature    3559..3578 
                     /label="Restriction Enzyme"  
     misc_feature    4363..5035 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Kan/Neo"  
     misc_feature    6294..6596 
                     /label="SV40 ori"  
     misc_feature    6682..7159 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3363..3369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3363 
                     /label="CLCN7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAACGTC TCTAAGAAGG TGTCCTGGTC CGGCCGGGAC CGGGACGACG 
     1081 AGGAGGCGGC GCCGCTGCTG CGGAGGACGG CGCGGCCCGG CGGGGGGACG CCGCTGCTGA 
     1141 ACGGGGCTGG GCCTGGGGCT GCGCGCCAGG ATATGGACCC TCCACATCCC TTCCCCAAGG 
     1201 AGATCCCACA CAACGAGAAG CTCCTGTCCC TCAAGTATGA GAGCTTGGAC TATGACAACA 
     1261 GTGAGAACCA GCTGTTCCTG GAGGAGGAGC GGCGGATCAA TCACACGGCC TTCCGGACGG 
     1321 TGGAGATCAA GCGCTGGGTC ATCTGCGCCC TCATTGGGAT CCTCACGGGC CTCGTGGCCT 
     1381 GCTTCATTGA CATCGTGGTG GAAAACCTGG CTGGCCTCAA GTACAGGGTC ATCAAGGGCA 
     1441 ATATCGACAA GTTCACAGAG AAGGGCGGAC TGTCCTTCTC CCTGTTGCTG TGGGCCACGC 
     1501 TGAACGCCGC CTTCGTGCTC GTGGGCTCTG TGATTGTGGC TTTCATAGAG CCGGTGGCTG 
     1561 CTGGCAGCGG AATCCCCCAG ATCAAGTGCT TCCTCAACGG GGTGAAGATC CCCCACGTGG 
     1621 TGCGGCTCAA GACGTTGGTG ATCAAAGTGT CCGGTGTGAT CCTGTCCGTG GTCGGGGGCC 
     1681 TGGCCGTGGG AAAGGAAGGG CCGATGATCC ACTCAGGTTC AGTGATTGCC GCCGGGATCT 
     1741 CTCAGGGAAG GTCAACGTCA CTGAAACGAG ATTTCAAGAT CTTCGAGTAC TTCCGCAGAG 
     1801 ACACAGAGAA GCGGGACTTC GTCTCCGCAG GGGCTGCGGC CGGAGTGTCA GCGGCGTTTG 
     1861 GAGCCCCCGT GGGTGGGGTC CTGTTCAGCT TGGAGGAGGG TGCGTCCTTC TGGAACCAGT 
     1921 TCCTGACCTG GAGGATCTTC TTTGCTTCCA TGATCTCCAC GTTCACCCTG AATTTTGTTC 
     1981 TGAGCATTTA CCACGGGAAC ATGTGGGACC TGTCCAGCCC AGGCCTCATC AACTTCGGAA 
     2041 GGTTTGACTC GGAGAAAATG GCCTACACGA TCCACGAGAT CCCGGTCTTC ATCGCCATGG 
     2101 GCGTGGTGGG CGGTGTGCTT GGAGCAGTGT TCAATGCCTT GAACTACTGG CTGACCATGT 
     2161 TTCGAATCAG GTACATCCAC CGGCCCTGCC TGCAGGTGAT TGAGGCCGTG CTGGTGGCCG 
     2221 CCGTCACGGC CACAGTTGCC TTCGTGCTGA TCTACTCGTC GCGGGATTGC CAGCCCCTGC 
     2281 AGGGGGGCTC CATGTCCTAC CCGCTGCAGC TCTTTTGTGC AGATGGCGAG TACAACTCCA 
     2341 TGGCTGCGGC CTTCTTCAAC ACCCCGGAGA AGAGCGTGGT GAGCCTCTTC CACGACCCGC 
     2401 CAGGCTCCTA CAACCCCCTG ACCCTCGGCC TGTTCACGCT GGTCTACTTC TTCCTGGCCT 
     2461 GCTGGACCTA CGGGCTCACG GTGTCTGCCG GGGTCTTCAT CCCGTCCCTG CTCATCGGGG 
     2521 CTGCCTGGGG CCGGCTCTTT GGGATCTCCC TGTCCTACCT CACGGGGGCG GCGATCTGGG 
     2581 CGGACCCCGG CAAATACGCC CTGATGGGAG CTGCTGCCCA GCTGGGCGGG ATTGTGCGGA 
     2641 TGACACTGAG CCTGACCGTC ATCATGATGG AGGCCACCAG CAACGTGACC TACGGCTTCC 
     2701 CCATCATGCT GGTGCTCATG ACCGCCAAGA TCGTGGGCGA CGTCTTCATT GAGGGCCTGT 
     2761 ACGACATGCA CATTCAGCTG CAGAGTGTGC CCTTCCTGCA CTGGGAGGCC CCGGTCACCT 
     2821 CACACTCACT CACTGCCAGG GAGGTGATGA GCACACCAGT GACCTGCCTG AGGCGGCGTG 
     2881 AGAAGGTCGG CGTCATTGTG GACGTGCTGA GCGACACGGC GTCCAATCAC AACGGCTTCC 
     2941 CCGTGGTGGA GCATGCCGAT GACACCCAGC CTGCCCGGCT CCAGGGCCTG ATCCTGCGCT 
     3001 CCCAGCTCAT CGTTCTCCTA AAGCACAAGG TGTTTGTGGA GCGGTCCAAC CTGGGCCTGG 
     3061 TACAGCGGCG CCTGAGGCTG AAGGACTTCC GAGACGCCTA CCCGCGCTTC CCACCCATCC 
     3121 AGTCCATCCA CGTGTCCCAG GACGAGCGGG AGTGCACCAT GGACCTCTCC GAGTTCATGA 
     3181 ACCCCTCCCC CTACACGGTG CCCCAGGAGG CGTCGCTCCC ACGGGTGTTC AAGCTGTTCC 
     3241 GGGCCCTGGG CCTGCGGCAC CTGGTGGTGG TGGACAACCG CAATCAGGTT GTCGGGTTGG 
     3301 TGACCAGGAA GGACCTCGCC AGGTACCGCC TGGGAAAGAG AGGCTTGGAG GAGCTCTCGC 
     3361 TGGCCCAGAC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3421 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3481 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3541 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3601 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3661 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3721 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3781 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3841 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3901 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3961 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4021 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4081 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4141 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5041 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5101 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5161 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5221 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5281 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5341 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5401 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5461 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5521 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5581 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5641 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5701 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5761 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5821 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5881 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5941 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6001 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6061 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6121 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6181 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6241 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6301 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6361 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6421 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6661 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6781 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6841 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6901 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6961 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7021 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7081 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7141 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7201 TAACAAAAAT TTAACGCGAA TTTT
//