LOCUS       RC226162                7224 bp    DNA     circular  SYN 
DEFINITION  PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B)
ACCESSION   NM_001114633
VERSION     NM_001114633.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3381..3472 
                     /label="Myc-DDK"  
     misc_feature    3502..4087 
                     /label="PolyA signal"  
     misc_feature    3559..3578 
                     /label="Restriction Enzyme"  
     misc_feature    4363..5035 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Kan/Neo"  
     misc_feature    6294..6596 
                     /label="SV40 ori"  
     misc_feature    6682..7159 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3363..3369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3363 
                     /label="PLA2G4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGGCA GAGGTGTCCA GGACCTGCCT GCTCACGGTT CGTGTCCTGC 
     1081 AGGCCCATCG CCTACCCTCT AAGGACCTAG TGACCCCCTC TGACTGCTAC GTGACTCTCT 
     1141 GGCTGCCCAC GGCCTGCAGC CACAGGCTCC AGACACGCAC GGTCAAGAAC AGCAGTAGCC 
     1201 CTGTCTGGAA CCAGAGCTTT CACTTCAGGA TCCACAGGCA GCTCAAGAAT GTCATGGAAC 
     1261 TGAAAGTCTT TGACCAGGAC CTGGTGACCG GAGATGACCC TGTGTTGTCA GTACTGTTTG 
     1321 ATGCGGGGAC TCTGCGGGCT GGGGAGTTCC GGCGCGAGAG CTTCTCACTG AGCCCTCAGG 
     1381 GTGAGGGGCG CCTGGAAGTT GAATTTCGCC TGCAGAGTCT GGCTGACCGT GGCGAGTGGC 
     1441 TCGTCAGCAA TGGCGTTCTG GTGGCCCGGG AGCTCTCCTG CTTGCACGTT CAACTGGAGG 
     1501 AGACAGGAGA CCAGAAGTCC TCAGAGCACA GAGTTCAGCT TGTGGTTCCT GGGTCCTGTG 
     1561 AGGGTCCGCA GGAGGCCTCT GTGGGCACTG GCACCTTCCG CTTCCACTGC CCAGCCTGCT 
     1621 GGGAGCAGGA GCTGAGTATT CGCCTGCAGG ATGCCCCCGA GGAGCAACTA AAGGCGCCAC 
     1681 TGAGTGCCCT GCCCTCTGGT CAAGTGGTGA GGCTTGTCTT CCCCACGTCC CAGGAGCCCC 
     1741 TGATGAGAGT GGAGCTGAAA AAAGAAGCAG GACTGAGGGA GCTGGCCGTG CGACTGGGCT 
     1801 TCGGGCCCTG TGCAGAGGAG CAGGCCTTCC TGAGCAGGAG GAAGCAGGTG GTGGCCGCGG 
     1861 CCTTGAGGCA GGCCCTGCAG CTGGACGGAG ACCTGCAGGA GGATGAGATC CCAGTGGTAG 
     1921 CTATTATGGC CACTGGTGGT GGGATCCGGG CAATGACTTC CCTGTATGGG CAGCTGGCTG 
     1981 GCCTGAAGGA GCTGGGCCTC TTGGATTGCG TCTCCTACAT CACCGGGGCC TCGGGCTCCA 
     2041 CCTGGGCCTT GGCCAACCTT TATGAGGACC CAGAGTGGTC TCAGAAGGAC CTGGCAGGGC 
     2101 CCACTGAGTT GCTGAAGACC CAGGTGACCA AGAACAAGCT GGGTGTGCTG GCCCCCAGCC 
     2161 AGCTGCAGCG GTACCGGCAG GAGCTGGCCG AGCGTGCCCG CTTGGGCTAC CCAAGCTGCT 
     2221 TCACCAACCT GTGGGCCCTC ATCAACGAGG CGCTGCTGCA TGATGAGCCC CATGATCACA 
     2281 AGCTCTCAGA TCAACGGGAG GCCCTGAGTC ATGGCCAGAA CCCTCTGCCC ATCTACTGTG 
     2341 CCCTCAACAC CAAAGGGCAG AGCCTGACCA CTTTTGAATT TGGGGAGTGG TGCGAGTTCT 
     2401 CTCCCTACGA GGTCGGCTTC CCCAAGTACG GGGCCTTCAT CCCCTCTGAG CTCTTTGGCT 
     2461 CCGAGTTCTT TATGGGGCAG CTGATGAAGA GGCTTCCTGA GTCCCGCATC TGCTTCTTAG 
     2521 AAGGTATCTG GAGCAACCTG TATGCAGCCA ACCTCCAGGA CAGCTTATAC TGGGCCTCAG 
     2581 AGCCCAGCCA GTTCTGGGAC CGCTGGGTCA GGAACCAGGC CAACCTGGAC AAGGAGCAGG 
     2641 TCCCCCTTCT GAAGATAGAA GAACCACCCT CAACAGCCGG CAGGATAGCT GAGTTTTTCA 
     2701 CCGATCTTCT GACGTGGCGT CCACTGGCCC AGGCCACACA TAATTTCCTG CGTGGCCTCC 
     2761 ATTTCCACAA AGACTACTTT CAGCATCCTC ACTTCTCCAC ATGGAAAGCT ACCACTCTGG 
     2821 ATGGGCTCCC CAACCAGCTG ACACCCTCGG AGCCCCACCT GTGCCTGCTG GATGTTGGCT 
     2881 ACCTCATCAA TACCAGCTGC CTGCCCCTCC TGCAGCCCAC TCGGGACGTG GACCTCATCC 
     2941 TGTCATTGGA CTACAACCTC CACGGAGCCT TCCAGCAGTT GCAGCTCCTG GGCCGGTTCT 
     3001 GCCAGGAGCA GGGGATCCCG TTCCCACCCA TCTCGCCCAG CCCCGAAGAG CAGCTCCAGC 
     3061 CTCGGGAGTG CCACACCTTC TCCGACCCCA CCTGCCCCGG AGCCCCTGCG GTGCTGCACT 
     3121 TTCCTCTGGT CAGCGACTCC TTCCGGGAGT ACTCGGCCCC TGGGGTCCGG CGGACACCCG 
     3181 AGGAGGCGGC AGCTGGGGAG GTGAACCTGT CTTCATCGGA CTCTCCCTAC CACTACACGA 
     3241 AGGTGACCTA CAGCCAGGAG GACGTGGACA AGCTGCTGCA CCTGACACAT TACAATGTCT 
     3301 GCAACAACCA GGAGCAGCTG CTGGAGGCTC TGCGCCAGGC AGTGCAGCGG AGGCGGCAGC 
     3361 GCAGGCCCCA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3421 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3481 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3541 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3601 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3661 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3721 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3781 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3841 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3901 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3961 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4021 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4081 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4141 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5041 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5101 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5161 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5221 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5281 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5341 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5401 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5461 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5521 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5581 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5641 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5701 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5761 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5821 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5881 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5941 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6001 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6061 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6121 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6181 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6241 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6301 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6361 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6421 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6661 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6781 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6841 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6901 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6961 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7021 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7081 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7141 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7201 TAACAAAAAT TTAACGCGAA TTTT
//