LOCUS       RC226161                7212 bp    DNA     circular  SYN 
DEFINITION  PLCD1 (Myc-DDK-tagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 1
ACCESSION   NM_001130964
VERSION     NM_001130964.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3369..3460 
                     /label="Myc-DDK"  
     misc_feature    3490..4075 
                     /label="PolyA signal"  
     misc_feature    3547..3566 
                     /label="Restriction Enzyme"  
     misc_feature    4351..5023 
                     /label="ColE1"  
     misc_feature    5453..6247 
                     /label="Kan/Neo"  
     misc_feature    6282..6584 
                     /label="SV40 ori"  
     misc_feature    6670..7147 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3351..3357 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3351 
                     /label="PLCD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTGCCTG GGGATCCGGA GCCGGAGCCG CTCCAGGGAG CTCTACCTGC 
     1081 AGGAGCGGAG CCTTAAGGTG GCGGCGCTCA ATGGACGGAG GCTGGGCCTA CAGGATGATG 
     1141 AGGATCTACA GGCGCTGCTG AAGGGCAGCC AGCTCCTGAA GGTGAAGTCC AGCTCATGGA 
     1201 GGAGAGAGCG CTTCTACAAG TTGCAGGAGG ACTGCAAGAC CATCTGGCAG GAGTCCCGCA 
     1261 AGGTCATGCG GACCCCGGAG TCCCAGCTGT TCTCCATCGA GGACATTCAG GAGGTGCGAA 
     1321 TGGGGCACCG CACGGAGGGT CTGGAGAAGT TCGCCCGTGA TGTGCCCGAG GACCGCTGCT 
     1381 TCTCCATTGT CTTCAAGGAC CAGCGCAATA CACTAGACCT CATCGCCCCA TCGCCAGCTG 
     1441 ATGCCCAGCA CTGGGTGCTG GGGCTGCACA AGATCATCCA CCACTCAGGC TCCATGGACC 
     1501 AGCGTCAGAA GCTACAGCAC TGGATTCACT CCTGCTTGCG AAAAGCTGAC AAAAACAAGG 
     1561 ACAACAAGAT GAGCTTCAAG GAGCTGCAGA ACTTCCTGAA GGAGCTCAAC ATCCAGGTGG 
     1621 ACGACAGCTA TGCCCGGAAG ATCTTCAGGG AGTGTGACCA CTCCCAGACA GACTCCCTGG 
     1681 AGGACGAGGA GATTGAGGCC TTCTACAAGA TGCTGACCCA GCGGGTGGAG ATCGACCGCA 
     1741 CCTTCGCCGA GGCCGCGGGC TCAGGGGAGA CTCTGTCGGT GGATCAGTTA GTGACGTTCC 
     1801 TGCAGCACCA GCAGCGGGAG GAGGCGGCAG GGCCTGCGCT GGCCCTCTCC CTCATTGAGC 
     1861 GCTACGAGCC CAGCGAGACT GCCAAGGCGC AGCGGCAGAT GACCAAGGAC GGCTTCCTCA 
     1921 TGTACTTACT GTCGGCTGAC GGCAGCGCCT TCAGCCTGGC ACACCGCCGT GTCTACCAGG 
     1981 ACATGGGCCA GCCACTTAGC CACTACCTGG TGTCCTCTTC ACACAACACC TACCTGCTGG 
     2041 AGGACCAGCT AGCCGGGCCC AGCAGCACTG AAGCCTACAT CCGGGCACTG TGCAAAGGCT 
     2101 GCCGATGCCT GGAGCTTGAC TGCTGGGACG GGCCCAACCA GGAACCAATC ATCTACCACG 
     2161 GCTATACTTT CACTTCCAAG ATCCTCTTCT GCGATGTGCT CAGGGCCATC CGGGACTATG 
     2221 CCTTCAAGGC GTCCCCCTAC CCTGTCATCC TATCCCTGGA GAACCACTGC ACACTGGAGC 
     2281 AGCAGCGCGT GATGGCGCGG CACCTGCATG CCATCCTGGG CCCCATGCTG TTGAACCGAC 
     2341 CACTGGATGG GGTCACCAAC AGCCTGCCCT CCCCTGAGCA ACTGAAGGGG AAGATCCTGC 
     2401 TGAAGGGGAA GAAGCTCGGG GGGCTCCTGC CCCCTGGAGG GGAGGGTGGC CCTGAGGCCA 
     2461 CTGTGGTGTC AGACGAAGAC GAGGCTGCTG AGATGGAGGA TGAGGCAGTG AGGAGCCGTG 
     2521 TGCAGCACAA GCCCAAGGAG GACAAGCTCA GGCTAGCACA GGAGCTCTCT GACATGGTCA 
     2581 TTTACTGCAA GAGTGTCCAC TTTGGGGGCT TCTCCAGTCC TGGCACCCCT GGACAGGCCT 
     2641 TCTACGAGAT GGCGTCCTTC TCTGAGAACC GTGCCCTTCG ACTGCTCCAA GAATCAGGAA 
     2701 ACGGCTTTGT CCGCCACAAC GTGGGGCACC TGAGCAGAAT CTACCCGGCT GGATGGAGAA 
     2761 CAGACTCCTC CAACTACAGC CCCGTGGAGA TGTGGAATGG GGGCTGCCAG ATCGTGGCCC 
     2821 TGAATTTCCA GACACCTGGG CCAGAGATGG ACGTGTACCA GGGCCGCTTC CAGGACAACG 
     2881 GGGCCTGTGG GTACGTGCTG AAGCCCGCCT TCCTGCGAGA CCCCAACGGC ACCTTTAACC 
     2941 CCCGCGCCCT GGCTCAGGGG CCCTGGTGGG CACGGAAGCG GCTCAACATC AGGGTCATTT 
     3001 CGGGGCAGCA GCTGCCAAAA GTCAACAAGA ATAAGAATTC AATTGTGGAC CCCAAAGTGA 
     3061 CAGTGGAGAT CCATGGCGTG AGCCGGGACG TGGCCAGCCG CCAGACTGCT GTCATCACCA 
     3121 ACAATGGTTT CAACCCATGG TGGGACACGG AGTTTGCGTT TGAGGTAGTT GTGCCTGACC 
     3181 TTGCCCTCAT CCGCTTCTTG GTGGAAGATT ATGATGCCTC CTCCAAGAAT GACTTCATTG 
     3241 GCCAGAGTAC CATCCCCTTG AACAGCCTCA AGCAAGGATA CCGCCATGTC CACCTCATGT 
     3301 CTAAGAACGG GGACCAGCAT CCATCAGCCA CCCTCTTTGT GAAGATCTCC CTCCAGGACA 
     3361 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3421 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3481 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3541 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3601 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3661 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3721 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3781 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3841 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3901 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3961 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4021 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4081 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4141 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4201 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4261 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4321 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4381 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4441 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4501 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4561 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4621 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4681 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4741 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4801 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4861 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4921 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4981 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5041 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5101 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5161 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5221 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5281 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5341 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5401 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5461 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5521 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5581 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5641 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5701 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5761 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5821 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5881 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5941 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6001 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6061 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6121 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6181 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6241 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6301 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6361 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6421 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6481 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6541 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6601 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6661 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6721 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6781 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6841 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6901 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7021 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7081 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7141 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7201 AACGCGAATT TT
//