LOCUS       RC226118                7191 bp    DNA     circular  SYN 
DEFINITION  GSN (Myc-DDK-tagged)-Human gelsolin (GSN), transcript variant 4
ACCESSION   NM_001127663
VERSION     NM_001127663.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3346..3437 
                     /label="Myc-DDK"  
     misc_feature    3467..4052 
                     /label="PolyA signal"  
     misc_feature    3524..3543 
                     /label="Restriction Enzyme"  
     misc_feature    4328..5000 
                     /label="ColE1"  
     misc_feature    5430..6224 
                     /label="Kan/Neo"  
     misc_feature    6259..6561 
                     /label="SV40 ori"  
     misc_feature    6647..7124 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3321..3328 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3321 
                     /label="GSN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATACAGGA CTTACGCGTC TGCTGTGGCC CAGCTGGCTT CCAGATGGTG 
     1081 ACATGAGCCA CCCACAGCCG GAGCTGTTCC TCTTTCCCAA AGCTCAGCCC AACAGCATGG 
     1141 TGGTGGAACA CCCCGAGTTC CTCAAGGCAG GGAAGGAGCC TGGCCTGCAG ATCTGGCGTG 
     1201 TGGAGAAGTT CGATCTGGTG CCCGTGCCCA CCAACCTTTA TGGAGACTTC TTCACGGGCG 
     1261 ACGCCTACGT CATCCTGAAG ACAGTGCAGC TGAGGAACGG AAATCTGCAG TATGACCTCC 
     1321 ACTACTGGCT GGGCAATGAG TGCAGCCAGG ATGAGAGCGG GGCGGCCGCC ATCTTTACCG 
     1381 TGCAGCTGGA TGACTACCTG AACGGCCGGG CCGTGCAGCA CCGTGAGGTC CAGGGCTTCG 
     1441 AGTCGGCCAC CTTCCTAGGC TACTTCAAGT CTGGCCTGAA GTACAAGAAA GGAGGTGTGG 
     1501 CATCAGGATT CAAGCACGTG GTACCCAACG AGGTGGTGGT GCAGAGACTC TTCCAGGTCA 
     1561 AAGGGCGGCG TGTGGTCCGT GCCACCGAGG TACCTGTGTC CTGGGAGAGC TTCAACAATG 
     1621 GCGACTGCTT CATCCTGGAC CTGGGCAACA ACATCCACCA GTGGTGTGGT TCCAACAGCA 
     1681 ATCGGTATGA AAGACTGAAG GCCACACAGG TGTCCAAGGG CATCCGGGAC AACGAGCGGA 
     1741 GTGGCCGGGC CCGAGTGCAC GTGTCTGAGG AGGGCACTGA GCCCGAGGCG ATGCTCCAGG 
     1801 TGCTGGGCCC CAAGCCGGCT CTGCCTGCAG GTACCGAGGA CACCGCCAAG GAGGATGCGG 
     1861 CCAACCGCAA GCTGGCCAAG CTCTACAAGG TCTCCAATGG TGCAGGGACC ATGTCCGTCT 
     1921 CCCTCGTGGC TGATGAGAAC CCCTTCGCCC AGGGGGCCCT GAAGTCAGAG GACTGCTTCA 
     1981 TCCTGGACCA CGGCAAAGAT GGGAAAATCT TTGTCTGGAA AGGCAAGCAG GCAAACACGG 
     2041 AGGAGAGGAA GGCTGCCCTC AAAACAGCCT CTGACTTCAT CACCAAGATG GACTACCCCA 
     2101 AGCAGACTCA GGTCTCGGTC CTTCCTGAGG GCGGTGAGAC CCCACTGTTC AAGCAGTTCT 
     2161 TCAAGAACTG GCGGGACCCA GACCAGACAG ATGGCCTGGG CTTGTCCTAC CTTTCCAGCC 
     2221 ATATCGCCAA CGTGGAGCGG GTGCCCTTCG ACGCCGCCAC CCTGCACACC TCCACTGCCA 
     2281 TGGCCGCCCA GCACGGCATG GATGACGATG GCACAGGCCA GAAACAGATC TGGAGAATCG 
     2341 AAGGTTCCAA CAAGGTGCCC GTGGACCCTG CCACATATGG ACAGTTCTAT GGAGGCGACA 
     2401 GCTACATCAT TCTGTACAAC TACCGCCATG GTGGCCGCCA GGGGCAGATA ATCTATAACT 
     2461 GGCAGGGTGC CCAGTCTACC CAGGATGAGG TCGCTGCATC TGCCATCCTG ACTGCTCAGC 
     2521 TGGATGAGGA GCTGGGAGGT ACCCCTGTCC AGAGCCGTGT GGTCCAAGGC AAGGAGCCCG 
     2581 CCCACCTCAT GAGCCTGTTT GGTGGGAAGC CCATGATCAT CTACAAGGGC GGCACCTCCC 
     2641 GCGAGGGCGG GCAGACAGCC CCTGCCAGCA CCCGCCTCTT CCAGGTCCGC GCCAACAGCG 
     2701 CTGGAGCCAC CCGGGCTGTT GAGGTATTGC CTAAGGCTGG TGCACTGAAC TCCAACGATG 
     2761 CCTTTGTTCT GAAAACCCCC TCAGCCGCCT ACCTGTGGGT GGGTACAGGA GCCAGCGAGG 
     2821 CAGAGAAGAC GGGGGCCCAG GAGCTGCTCA GGGTGCTGCG GGCCCAACCT GTGCAGGTGG 
     2881 CAGAAGGCAG CGAGCCAGAT GGCTTCTGGG AGGCCCTGGG CGGGAAGGCT GCCTACCGCA 
     2941 CATCCCCACG GCTGAAGGAC AAGAAGATGG ATGCCCATCC TCCTCGCCTC TTTGCCTGCT 
     3001 CCAACAAGAT TGGACGTTTT GTGATCGAAG AGGTTCCTGG TGAGCTCATG CAGGAAGACC 
     3061 TGGCAACGGA TGACGTCATG CTTCTGGACA CCTGGGACCA GGTCTTTGTC TGGGTTGGAA 
     3121 AGGATTCTCA AGAAGAAGAA AAGACAGAAG CCTTGACTTC TGCTAAGCGG TACATCGAGA 
     3181 CGGACCCAGC CAATCGGGAT CGGCGGACGC CCATCACCGT GGTGAAGCAA GGCTTTGAGC 
     3241 CTCCCTCCTT TGTGGGCTGG TTCCTTGGCT GGGATGATGA TTACTGGTCT GTGGACCCCT 
     3301 TGGACAGGGC CATGGCTGAG CTGGCTGCCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     3361 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3421 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3481 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3541 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3601 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3661 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3721 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3781 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3841 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3901 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3961 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4021 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4081 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4141 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4201 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4261 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4321 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4381 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4441 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4501 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4561 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4621 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4681 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4741 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4801 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4861 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4921 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4981 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5041 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5101 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5161 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5221 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5281 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5341 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5401 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5461 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5521 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5581 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5641 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5701 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5761 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5821 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5881 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5941 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6001 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6061 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6121 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6181 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6241 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6301 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6361 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6421 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6481 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6541 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6601 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6661 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6721 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6781 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6841 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6901 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6961 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7021 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7081 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7141 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//