LOCUS       RC226075                6876 bp    DNA     circular  SYN 
DEFINITION  LRRC32 (Myc-DDK-tagged)-Human leucine rich repeat containing 32 (LRRC32), transcript variant 2
ACCESSION   NM_001128922
VERSION     NM_001128922.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3031..3122 
                     /label="Myc-DDK"  
     misc_feature    3152..3737 
                     /label="PolyA signal"  
     misc_feature    3209..3228 
                     /label="Restriction Enzyme"  
     misc_feature    4013..4685 
                     /label="ColE1"  
     misc_feature    5115..5909 
                     /label="Kan/Neo"  
     misc_feature    5944..6246 
                     /label="SV40 ori"  
     misc_feature    6332..6809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3006..3013 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3006 
                     /label="LRRC32"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACCCCAG ATCCTGCTGC TCCTGGCCCT GCTGACCCTA GGCCTGGCTG 
     1081 CACAACACCA AGACAAAGTG CCCTGTAAGA TGGTGGACAA GAAGGTCTCG TGCCAGGTTC 
     1141 TGGGCCTGCT CCAGGTCCCC TCGGTGCTCC CGCCAGACAC TGAGACCCTT GATCTATCTG 
     1201 GGAACCAGCT GCGGAGTATC CTGGCCTCAC CCCTGGGCTT CTACACGGCA CTTCGTCACC 
     1261 TGGACCTGAG CACCAATGAG ATCAGCTTCC TCCAGCCAGG AGCCTTCCAG GCCCTGACCC 
     1321 ACCTGGAGCA CCTCAGCCTG GCTCACAACC GGCTGGCGAT GGCCACTGCG CTGAGTGCTG 
     1381 GTGGCCTGGG CCCCCTGCCA CGCGTGACCT CCCTGGACCT GTCTGGGAAC AGCCTGTACA 
     1441 GCGGCCTGCT GGAGCGGCTG CTGGGGGAGG CACCCAGCCT GCATACCCTC TCACTGGCGG 
     1501 AGAACAGTCT GACTCGCCTC ACCCGCCACA CCTTCCGGGA CATGCCTGCG CTGGAGCAGC 
     1561 TTGACCTGCA TAGCAACGTG CTGATGGACA TCGAGGATGG CGCCTTCGAG GGTCTGCCCC 
     1621 GCCTGACCCA TCTCAACCTC TCCAGGAATT CCCTCACCTG CATCTCCGAC TTCAGCCTCC 
     1681 AGCAGCTGCG GGTGCTAGAC CTGAGCTGCA ACAGCATCGA GGCCTTTCAG ACGGCCTCCC 
     1741 AGCCCCAGGC TGAGTTCCAG CTCACCTGGC TTGACCTGCG GGAGAACAAA CTGCTCCATT 
     1801 TCCCCGACCT GGCCGCGCTC CCGAGACTCA TCTACCTGAA CTTGTCCAAC AACCTCATCC 
     1861 GGCTCCCCAC AGGGCCACCC CAGGACAGCA AGGGCATCCA CGCACCTTCC GAGGGCTGGT 
     1921 CAGCCCTGCC CCTCTCAGCC CCCAGCGGGA ATGCCAGCGG CCGCCCCCTT TCCCAGCTCT 
     1981 TGAATCTGGA TTTGAGCTAC AATGAGATTG AGCTCATCCC CGACAGCTTT CTTGAGCACC 
     2041 TGACCTCCCT GTGCTTCCTG AACCTCAGCA GAAACTGCTT GCGGACCTTT GAGGCCCGGC 
     2101 GCTTAGGCTC CCTGCCCTGC CTGATGCTCC TTGACTTAAG CCACAATGCC CTGGAGACAC 
     2161 TGGAACTGGG CGCCAGAGCC CTGGGGTCTC TGCGGACGCT GCTCCTACAG GGCAATGCCC 
     2221 TGCGGGACCT GCCCCCATAC ACCTTTGCCA ATCTGGCCAG CCTGCAGCGG CTCAACCTGC 
     2281 AGGGGAACCG AGTCAGCCCC TGTGGGGGGC CAGATGAGCC TGGCCCCTCC GGCTGTGTGG 
     2341 CCTTCTCCGG CATCACCTCC CTCCGCAGCC TGAGCCTGGT GGATAATGAG ATAGAGCTGC 
     2401 TCAGGGCAGG GGCCTTCCTC CACACCCCAC TGACTGAGCT GGACCTTTCT TCCAATCCTG 
     2461 GGCTGGAGGT GGCCACGGGG GCCTTGGGAG GCCTGGAGGC CTCCTTGGAG GTCCTGGCAC 
     2521 TGCAGGGCAA CGGGCTGATG GTCCTGCAGG TGGACCTGCC CTGCTTCATC TGCCTCAAGC 
     2581 GGCTCAATCT TGCCGAGAAC CGCCTGAGCC ACCTTCCCGC CTGGACACAG GCTGTGTCAC 
     2641 TGGAGGTGCT GGACCTGCGA AACAACAGCT TCAGCCTCCT GCCAGGCAGT GCCATGGGTG 
     2701 GCCTGGAGAC CAGCCTCCGG CGCCTCTACC TGCAGGGGAA TCCACTCAGC TGCTGCGGCA 
     2761 ATGGCTGGCT GGCAGCCCAG CTGCACCAGG GCCGTGTGGA CGTGGACGCC ACCCAGGACC 
     2821 TGATCTGCCG CTTCAGCTCC CAGGAGGAGG TGTCCCTGAG CCACGTGCGT CCCGAAGACT 
     2881 GTGAGAAGGG GGGACTGAAG AACATCAACC TCATCATCAT CCTCACCTTC ATACTGGTCT 
     2941 CTGCCATCCT CCTCACCACG CTGGCCGCCT GCTGCTGCGT CCGCCGGCAG AAGTTTAACC 
     3001 AACAGTATAA AGCCAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3061 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3121 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3181 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3241 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3301 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3361 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3421 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3481 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3541 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3601 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3661 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3721 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3781 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3841 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3901 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3961 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4021 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4081 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4141 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4201 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4261 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4321 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4381 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4441 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4501 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4561 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4621 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4681 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4741 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4801 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4861 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4921 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4981 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5041 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5101 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5161 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5221 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5281 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5341 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5401 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5461 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5521 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5581 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5641 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5701 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5761 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5821 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5881 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5941 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6001 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6061 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6121 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6181 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6241 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6301 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6361 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6421 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6481 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6541 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6601 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6661 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6721 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6781 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6841 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//