LOCUS       RC226028                6735 bp    DNA     circular  SYN 
DEFINITION  KLC1 (Myc-DDK-tagged)-Human kinesin light chain 1 (KLC1), transcript variant 3
ACCESSION   NM_001130107
VERSION     NM_001130107.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2892..2983 
                     /label="Myc-DDK"  
     misc_feature    3013..3598 
                     /label="PolyA signal"  
     misc_feature    3070..3089 
                     /label="Restriction Enzyme"  
     misc_feature    3874..4546 
                     /label="ColE1"  
     misc_feature    4976..5770 
                     /label="Kan/Neo"  
     misc_feature    5805..6107 
                     /label="SV40 ori"  
     misc_feature    6193..6670 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2874..2880 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2874 
                     /label="KLC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGACAAC ATGTCCACAA TGGTGTACAT AAAGGAAGAC AAGTTGGAGA 
     1081 AGCTTACACA GGATGAAATT ATTTCTAAGA CAAAGCAAGT AATTCAGGGG CTGGAAGCTT 
     1141 TGAAGAATGA GCACAATTCC ATTTTACAAA GTTTGCTGGA GACACTGAAG TGTTTGAAGA 
     1201 AAGATGATGA AAGTAATTTG GTGGAGGAGA AATCAAACAT GATCCGGAAG TCACTGGAGA 
     1261 TGTTGGAGCT CGGCCTGAGT GAGGCACAGG TTATGATGGC TTTGTCAAAT CACCTGAATG 
     1321 CTGTGGAGTC CGAGAAGCAG AAACTGCGTG CGCAGGTTCG TCGTCTGTGC CAGGAGAATC 
     1381 AGTGGCTACG GGATGAACTG GCCAACACGC AGCAGAAACT GCAGAAGAGT GAGCAGTCTG 
     1441 TGGCTCAACT GGAGGAGGAG AAGAAGCATC TGGAGTTTAT GAATCAGCTA AAAAAATATG 
     1501 ATGACGACAT TTCCCCATCC GAGGACAAAG ACACTGATTC TACCAAAGAG CCTCTGGATG 
     1561 ACCTTTTCCC CAATGATGAA GACGACCCAG GGCAAGGAAT CCAGCAGCAG CACAGCAGTG 
     1621 CAGCCGCGGC TGCCCAGCAG GGCGGCTACG AGATCCCCGC GCGGCTGCGG ACGCTCCACA 
     1681 ACCTGGTGAT CCAGTACGCC TCGCAGGGGC GCTACGAGGT AGCTGTGCCC CTCTGCAAGC 
     1741 AGGCCCTGGA GGACCTGGAG AAGACTTCAG GACACGACCA CCCGGACGTG GCCACCATGC 
     1801 TCAACATCCT GGCCTTGGTG TACAGGGATC AGAATAAATA CAAAGATGCA GCTAACCTAC 
     1861 TGAATGATGC CTTGGCTATT CGTGAGAAAA CTTTGGGCAA AGATCATCCT GCGGTGGCGG 
     1921 CGACTTTGAA TAACCTTGCA GTCCTTTATG GTAAAAGAGG GAAGTACAAA GAAGCAGAGC 
     1981 CGTTGTGTAA AAGAGCTCTG GAAATCCGAG AAAAGGTTTT GGGGAAGGAT CACCCCGATG 
     2041 TTGCCAAGCA GTTAAATAAC TTGGCCTTAC TGTGCCAGAA CCAGGGCAAG TATGAAGAAG 
     2101 TAGAATATTA TTATCAAAGA GCCCTCGAGA TCTACCAGAC AAAACTGGGA CCTGATGACC 
     2161 CCAACGTGGC TAAGACGAAA AATAACCTGG CATCCTGCTA TTTGAAACAA GGAAAGTTCA 
     2221 AGCAAGCAGA AACACTGTAC AAAGAGATTC TCACTCGTGC ACATGAAAGG GAGTTTGGTT 
     2281 CTGTAGATGA TGAAAATAAA CCCATCTGGA TGCATGCTGA AGAAAGAGAA GAATGCAAAG 
     2341 GAAAGCAAAA GGATGGGACA TCTTTTGGAG AGTATGGCGG CTGGTACAAA GCCTGCAAAG 
     2401 TTGATAGTCC AACTGTTACA ACCACTCTAA AAAACCTTGG GGCACTTTAC AGACGTCAAG 
     2461 GCAAATTTGA AGCTGCAGAA ACGTTAGAAG AAGCTGCTAT GAGGTCTCGT AAACAGGGTC 
     2521 TTGACAATGT TCACAAACAG AGGGTGGCAG AAGTGCTCAA TGACCCTGAG AACATGGAGA 
     2581 AGCGCAGGAG CCGTGAGAGC CTCAACGTGG ACGTGGTCAA GTACGAGAGT GGCCCTGACG 
     2641 GAGGGGAGGA AGTGAGTATG AGCGTAGAGT GGAACGGGGA TGGCACTGGA TCTTTAAAAC 
     2701 GCAGTGGTTC CTTTAGCAAA CTCCGGGCTT CCATTAGACG CAGCAGTGAG AAGCTGGTTA 
     2761 GGAAGCTGAA GGGAGGAAGT TCACGAGAGA GTGAGCCAAA GAACCCCGGG GCGTCTCTGG 
     2821 CCGAGCCTCT TTTTGTGGAA AACGACAGCA GCAGCAGTGG CCTGGAAGAC GCCACCGCTA 
     2881 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     2941 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3001 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3061 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3121 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3181 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3241 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3301 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3361 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3421 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3481 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3541 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3601 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3661 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3721 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     3781 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     3841 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     3901 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     3961 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4021 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4081 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4141 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4201 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4261 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4321 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4381 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4441 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4501 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4561 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4621 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4681 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4741 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     4801 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     4861 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     4921 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     4981 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5041 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5101 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5161 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5221 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5281 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5341 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5401 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5461 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5521 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5581 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5641 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5701 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     5761 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     5821 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     5881 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     5941 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6001 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6061 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6121 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6181 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6241 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6301 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6361 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6421 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6481 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6541 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6601 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6661 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6721 TTTAACGCGA ATTTT
//