LOCUS       RC225875L4                9236 bp    DNA     circular  SYN 
DEFINITION  VRK2 (Myc-DDK-tagged)-Human vaccinia related kinase 2 (VRK2), transcript variant 3
ACCESSION   NM_001130481
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7527..7707 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8435..9081 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6402..6422 
                     /label="LR50"  
     misc_feature    4865..5593 
                     /label="mGFP"  
     misc_feature    5600..5665 
                     /label="P2A"  
     misc_feature    7755..8374 
                     /label="pBR322 Ori"  
     misc_feature    5666..6265 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4844..4850 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4844 
                     /label="VRK2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCACCAAAA AGAAATGAAA AATACAAACT 
     3361 TCCTATTCCA TTTCCAGAAG GCAAGGTTCT GGATGATATG GAAGGCAATC AGTGGGTACT 
     3421 GGGCAAGAAG ATTGGCTCTG GAGGATTTGG ATTGATATAT TTAGCTTTCC CCACAAATAA 
     3481 ACCAGAGAAA GATGCAAGAC ATGTAGTAAA AGTGGAATAT CAAGAAAATG GCCCGTTATT 
     3541 TTCAGAACTT AAATTTTATC AGAGAGTTGC AAAAAAAGAC TGTATCAAAA AGTGGATAGA 
     3601 ACGCAAACAA CTTGATTATT TAGGAATTCC TCTGTTTTAT GGATCTGGTC TGACTGAATT 
     3661 CAAGGGAAGA AGTTACAGAT TTATGGTAAT GGAAAGACTA GGAATAGATT TACAGAAGAT 
     3721 CTCAGGCCAG AATGGTACCT TTAAAAAGTC AACTGTCCTG CAATTAGGTA TCCGAATGTT 
     3781 GGATGTACTG GAATATATAC ATGAAAATGA ATATGTTCAT GGTGATGTAA AAGCAGCAAA 
     3841 TCTACTTTTG GGTTACAAAA ATCCAGACCA GGTTTATCTT GCAGATTATG GACTTTCCTA 
     3901 CAGATATTGT CCCAATGGGA ACCACAAACA GTATCAGGAA AATCCTAGAA AAGGCCATAA 
     3961 TGGGACAATA GAGTTTACCA GCTTGGATGC CCACAAGGGA GTAGCCTTGT CCAGACGAAG 
     4021 TGACGTTGAG ATCCTCGGCT ACTGCATGCT GCGGTGGTTG TGTGGGAAAC TTCCCTGGGA 
     4081 ACAGAACCTG AAGGACCCTG TGGCTGTGCA GACTGCTAAA ACAAATCTGT TGGACGAGCT 
     4141 CCCCCAGTCA GTGCTTAAAT GGGCTCCATC TGGAAGCAGT TGCTGTGAAA TAGCCCAATT 
     4201 TTTGGTATGT GCTCATAGTT TAGCATATGA TGAAAAGCCA AACTATCAAG CCCTCAAGAA 
     4261 AATTTTGAAC CCTCATGGAA TACCTTTAGG ACCACTGGAC TTTTCCACAA AAGGACAGAG 
     4321 TATAAATGTC CATACTCCAA ACAGTCAAAA AGTTGATTCA CAAAAGGCTG CAACAAAGCA 
     4381 AGTCAACAAG GCACACAATA GGTTAATCGA AAAAAAAGTC CACAGTGAGA GAAGCGCTGA 
     4441 GTCCTGTGCA ACATGGAAAG TGCAGAAAGA GGAGAAACTG ATTGGATTGA TGAACAATGA 
     4501 AGCAGCTCAG GAAAGCACAA GGAGAAGACA GAAATATCAA GAGTCTCAAG AACCTTTGAA 
     4561 TGAAGTAAAC AGTTTCCCAC AAAAAATCAG CTATACACAA TTCCCAAACT CATTTTATGA 
     4621 GCCTCATCAA GATTTTACCA GTCCAGATAT ATTCAAGAAG TCAAGATCTC CATCTTGGTA 
     4681 TAAATACACT TCCACAGTCA GCACGGGGAT CACAGACTTA GAAAGTTCAA CTGGACTTTG 
     4741 GCCTACAATT TCCCAGTTTA CTCTTAGTGA AGAGACAAAC GCAGATGTTT ATTATTATCG 
     4801 CATCATCATA CCTGTCCTTT TGATGTTAGT ATTTCTTGCT TTATTTTTTC TCACGCGTAC 
     4861 GCGGCCGCTC GAGATGAGCG GGGGCGAGGA GCTGTTCGCC GGCATCGTGC CCGTGCTGAT 
     4921 CGAGCTGGAC GGCGACGTGC ACGGCCACAA GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA 
     4981 CGCCGACTAC GGCAAGCTGG AGATCAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 
     5041 CTGGCCCACC CTGGTGACCA CCCTCTGCTA CGGCATCCAG TGCTTCGCCC GCTACCCCGA 
     5101 GCACATGAAG ATGAACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACA TCCAGGAGCG 
     5161 CACCATCCAG TTCCAGGACG ACGGCAAGTA CAAGACCCGC GGCGAGGTGA AGTTCGAGGG 
     5221 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCAAGGAC TTCAAGGAGG ACGGCAACAT 
     5281 CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA CAGCCACAAC GTGTACATCC GCCCCGACAA 
     5341 GGCCAACAAC GGCCTGGAGG CTAACTTCAA GACCCGCCAC AACATCGAGG GCGGCGGCGT 
     5401 GCAGCTGGCC GACCACTACC AGACCAACGT GCCCCTGGGC GACGGCCCCG TGCTGATCCC 
     5461 CATCAACCAC TACCTGAGCA CTCAGACCAA GATCAGCAAG GACCGCAACG AGGCCCGCGA 
     5521 CCACATGGTG CTCCTGGAGT CCTTCAGCGC CTGCTGCCAC ACCCACGGCA TGGACGAGCT 
     5581 GTACAGGTCC GGACTCAGAG TTTGGGTAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA 
     5641 GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTATGACC GAGTACAAGC CCACGGTGCG 
     5701 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 
     5761 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGATCGCCAC ATCGAGCGGG TCACCGAGCT 
     5821 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 
     5881 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 
     5941 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 
     6001 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 
     6061 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 
     6121 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 
     6181 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 
     6241 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATGTACA CCCAAACGGC CGGCCGCGGT 
     6301 CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 
     6361 TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA 
     6421 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 
     6481 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 
     6541 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 
     6601 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 
     6661 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 
     6721 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 
     6781 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 
     6841 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 
     6901 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 
     6961 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC 
     7021 GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC 
     7081 AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA 
     7141 CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA 
     7201 AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT 
     7261 CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA 
     7321 GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG 
     7381 AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT 
     7441 GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT 
     7501 CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC 
     7561 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 
     7621 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 
     7681 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA 
     7741 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 
     7801 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 
     7861 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 
     7921 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 
     7981 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 
     8041 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 
     8101 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 
     8161 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 
     8221 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 
     8281 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 
     8341 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 
     8401 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC 
     8461 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 
     8521 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 
     8581 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 
     8641 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG 
     8701 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 
     8761 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 
     8821 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 
     8881 TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 
     8941 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 
     9001 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 
     9061 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT 
     9121 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9181 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//