LOCUS       RC225655                6094 bp    DNA     circular  SYN 
DEFINITION  DMKN (Myc-DDK-tagged)-Human dermokine (DMKN), transcript variant 3
ACCESSION   NM_001126056
VERSION     NM_001126056.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2251..2342 
                     /label="Myc-DDK"  
     misc_feature    2372..2957 
                     /label="PolyA signal"  
     misc_feature    2429..2448 
                     /label="Restriction Enzyme"  
     misc_feature    3233..3905 
                     /label="ColE1"  
     misc_feature    4335..5129 
                     /label="Kan/Neo"  
     misc_feature    5164..5466 
                     /label="SV40 ori"  
     misc_feature    5552..6029 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2233..2239 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2233 
                     /label="DMKN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTTCCAG GGGCCCCTGG CCTGCCTCCT GCTGGCCCTC TGCCTGGGCA 
     1081 GTGGGGAGGC TGGCCCCCTG CAGAGCGGAG AGGAAAGCAC TGGGACAAAT ATTGGGGAGG 
     1141 CCCTTGGACA TGGCCTGGGA GACGCCCTGA GCGAAGGGGT GGGAAAGGCC ATTGGCAAAG 
     1201 AGGCCGGAGG GGCAGCTGGC TCTAAAGTCA GTGAGGCCCT TGGCCAAGGG ACCAGAGAAG 
     1261 CAGTTGGCAC TGGAGTCAGG CAGGTTCCAG GCTTTGGCGT AGCAGATGCT TTGGGCAACA 
     1321 GGGTCGGGGA AGCAGCCCAT GCTCTGGGAA ACACTGGGCA CGAGATTGGC AGACAGGCAG 
     1381 AAGATGTCAT TCGACACGGA GCAGATGCTG TCCGCGGCTC CTGGCAGGGG GTGCCTGGCC 
     1441 ACAATGGTGC TTGGGAAACT TCTGGAGGCC ATGGCATCTT TGGCTCTCAA GGTGGCCTTG 
     1501 GAGGCCAGGG CCAGGGCAAT CCTGGAGGTC TGGGGACTCC GTGGGTCCAC GGATACCCCG 
     1561 GAAACTCAGC AGGCAGCTTT GGAATGAATC CTCAGGGAGC TCCCTGGGGT CAAGGAGGCA 
     1621 ATGGAGGGCC ACCAAACTTT GGGACCAACA CTCAGGGAGC TGTGGCCCAG CCTGGCTATG 
     1681 GTTCAGTGAG AGCCAGCAAC CAGAATGAAG GGTGCACGAA TCCCCCACCA TCTGGCTCAG 
     1741 GTGGAGGCTC CAGCAACTCT GGGGGATCCA GCACCGGCTC CTCCTCCGGC AACCACGGTG 
     1801 GGAGCGGCGG AGGAAATGGA CATAAACCCG GGTGTGAAAA GCCAGGGAAT GAAGCCCGCG 
     1861 GGAGCGGGGA ATCTGGGATT CAGGGCTTCA GAGGACAGGG AGTTTCCAGC AACATGAGGG 
     1921 AAATAAGCAA AGAGGGCAAT CGCCTCCTTG GAGGCTCTGG AGACAATTAT AGGGGGCAAG 
     1981 GGTCGAGCTG GGGCAGTGGA GGAGGTGACG CTGTTGGTGG AGTCAATACT GTGAACTCTG 
     2041 AGACGTCTCC TGGGATGTTT AACTTTGACA CTTTCTGGAA GAATTTTAAA TCCAAGCTGG 
     2101 GTTTCATCAA CTGGGATGCC ATAAACAAGA ACCAGGTCCC GCCCCCCAGC ACCCGAGCCC 
     2161 TCCTCTACTT CAGCCGACTC TGGGAGGATT TCAAACAGAA CACTCCTTTC CTCAACTGGA 
     2221 AAGCAATTAT TGAGGGTGCG GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA 
     2281 GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG 
     2341 GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC 
     2401 CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT 
     2461 AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG 
     2521 GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA 
     2581 TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG 
     2641 GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC 
     2701 CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT 
     2761 GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT 
     2821 ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA 
     2881 GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG 
     2941 TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT 
     3001 GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     3061 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     3121 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     3181 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     3241 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     3301 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     3361 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     3421 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     3481 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     3541 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     3601 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     3661 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     3721 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     3781 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     3841 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG 
     3901 CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG 
     3961 GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG 
     4021 GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA 
     4081 CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG 
     4141 TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT 
     4201 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT 
     4261 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG 
     4321 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT 
     4381 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC 
     4441 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC 
     4501 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG 
     4561 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG 
     4621 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC 
     4681 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC 
     4741 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA 
     4801 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA 
     4861 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC 
     4921 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA 
     4981 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC 
     5041 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC 
     5101 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT 
     5161 CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA 
     5221 ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT 
     5281 GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG 
     5341 GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     5401 CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC 
     5461 CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT 
     5521 TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     5581 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     5641 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 
     5701 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 
     5761 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 
     5821 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 
     5881 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 
     5941 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 
     6001 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 
     6061 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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