LOCUS       RC224955                8340 bp    DNA     circular  SYN 
DEFINITION  KIF1B (Myc-DDK-tagged)-Human kinesin family member 1B (KIF1B), transcript variant 2
ACCESSION   NM_183416
VERSION     NM_183416.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="KIF1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGGAGCC TCAGTGAAGG TGGCTGTCCG GGTAAGGCCC TTCAATTCTC 
     1081 GAGAGACCAG CAAGGAATCC AAATGCATCA TTCAGATGCA AGGCAACTCG ACCAGTATTA 
     1141 TTAACCCAAA GAATCCAAAG GAAGCTCCAA AGTCCTTCAG CTTCGACTAT TCCTACTGGT 
     1201 CTCATACCTC ACCCGAAGAT CCCTGTTTTG CATCTCAAAA CCGTGTGTAC AATGACATTG 
     1261 GCAAGGAAAT GCTCTTACAC GCCTTTGAGG GATATAATGT CTGTATTTTT GCCTATGGGC 
     1321 AGACTGGTGC TGGAAAATCT TATACAATGA TGGGTAAACA AGAAGAAAGC CAGGCTGGCA 
     1381 TCATTCCACA GTTATGTGAA GAACTTTTTG AGAAAATCAA TGACAACTGT AATGAAGAAA 
     1441 TGTCTTACTC TGTAGAGGTG AGCTACATGG AAATTTACTG TGAAAGAGTA CGAGATTTGC 
     1501 TGAATCCAAA AAACAAGGGT AATTTGCGTG TGCGTGAACA CCCACTTCTT GGACCCTATG 
     1561 TGGAGGATCT GTCCAAGTTG GCAGTTACTT CCTACACAGA CATTGCTGAC CTCATGGATG 
     1621 CTGGGAACAA AGCCAGGACA GTGGCAGCTA CAAACATGAA TGAAACAAGT AGCCGTTCCC 
     1681 ACGCTGTGTT TACGATTGTT TTCACCCAGA AGAAACACGA TAATGAGACC AACCTTTCCA 
     1741 CTGAGAAGGT CAGTAAAATC AGCTTGGTGG ATCTAGCAGG AAGTGAACGA GCTGATTCAA 
     1801 CTGGTGCCAA AGGGACTCGA TTAAAGGAAG GAGCAAATAT TAATAAGTCT CTTACAACTT 
     1861 TGGGCAAAGT CATTTCAGCC TTGGCCGAGG TGAGTAAAAA GAAGAAGAAA ACAGATTTTA 
     1921 TTCCCTACAG GGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATTTAGGT GGCAATTCTC 
     1981 GGACTGCAAT GGTTGCTGCT CTGAGCCCCG CGGATATCAA CTACGATGAG ACTTTGAGCA 
     2041 CTCTGAGATA TGCAGATCGT GCAAAACAAA TTAAATGCAA TGCTGTTATC AATGAGGACC 
     2101 CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG GACCTTCTTC 
     2161 GTGCTCAGGG CCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTCACT TCATCCCCAT 
     2221 CTTCCTGCTC ACTCAGTAGT CAGGTGGGCT TGACGTCTGT GACCAGTATT CAAGAGAGGA 
     2281 TCATGTCTAC ACCTGGAGGA GAGGAAGCTA TTGAACGTTT AAAGGAATCA GAGAAGATCA 
     2341 TTGCTGAGTT GAATGAAACT TGGGAAGAGA AGCTTCGTAA AACAGAGGCC ATCAGAATGG 
     2401 AGAGAGAGGC TTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGATGGA GGAACCCTAG 
     2461 GGGTTTTCTC ACCTAAAAAG ACCCCACATC TTGTTAACCT CAATGAAGAC CCACTAATGT 
     2521 CTGAGTGCCT ACTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA GCAGATGCTG 
     2581 AGCGGCGCCA GGACATAGTG CTGAGCGGGG CTCACATTAA AGAAGAGCAT TGTATCTTCC 
     2641 GGAGTGAGAG AAGCAACAGC GGGGAAGTTA TCGTGACCTT AGAGCCCTGT GAGCGCTCAG 
     2701 AAACCTACGT AAATGGCAAG AGGGTGTCCC AGCCTGTTCA GCTGCGCTCA GGAAACCGTA 
     2761 TCATCATGGG TAAAAACCAT GTTTTCCGCT TTAACCACCC GGAACAAGCA CGAGCTGAGC 
     2821 GAGAGAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACA TTTGCCCAGA 
     2881 GGGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAAGA GATGGAGAAA AGGCTACAGG 
     2941 AAATGGAGAT CTTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG GAGCAGCAGA 
     3001 GACTGGACGC GGATTCTGAT AGCGGGGACG ATTCTGACAA GAGGTCGTGT GAAGAGAGCT 
     3061 GGAAACTGAT TACTTCTCTG AGAGAAAAGC TACCTCCCAG CAAGTTGCAA ACCATTGTTA 
     3121 AAAAATGTGG CCTCCCAAGC AGTGGGAAGA AACGTGAACC AATTAAAATG TATCAGATAC 
     3181 CCCAAAGAAG GCGCTTGAGT AAAGATTCCA AGTGGGTCAC AATCTCAGAT CTTAAAATTC 
     3241 AGGCTGTCAA AGAGATTTGC TATGAGGTTG CTCTCAATGA CTTCAGGCAC AGTCGGCAGG 
     3301 AGATTGAAGC CCTGGCCATT GTCAAGATGA AGGAGCTTTG TGCCATGTAT GGCAAGAAAG 
     3361 ACCCCAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA CGTCTGGGAT ACCGTCGGTG 
     3421 TTGGGGATGA GAAGATCGAA GACGTCATGG CCACTGGGAA AGGCAGCACT GATGTAGATG 
     3481 ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA AAGCAAAATA 
     3541 ACATGAAAGA CGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG GAAAAAGTCT 
     3601 TGCCACTGAT CGGATCTCAG GAACAGAAAA GCCCAGGAAG CCACAAAGCA AAGGAGCCTG 
     3661 TTGGTGCTGG TGTTAGTAGC ACCTCTGAGA ATAATGTAAG TAAAGGAGAC AATGGAGAAC 
     3721 TTGCAAAAGA AGAACGTGTT TCCCAGCTGA TGAATGGGGA TCCAGCTTTT AGACGTGGAC 
     3781 GTCTGCGCTG GATGAGGCAA GAGCAAATTC GGTTTAAGAA CTTGCAACAG CAGGAGATAA 
     3841 CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATAGGTTCAT CCCTCCTGAG AACCGGAAGC 
     3901 CCCGCTTCCC CTTTAAGAGC AACCCTAAAC ACAGAAACTC TTGGAGTCCT GGGACACATA 
     3961 TCATCATAAC AGAAGATGAG GTTATAGAGC TTAGGATTCC AAAAGACGAT GAAGCAAGGA 
     4021 AAGGGAATAA AGAAGAGAGC CAAGAAAAAG GGGGTAAAGG AGCTTTTAAG GATCCCCAGT 
     4081 TTCCATGGGG CTCTCAAGGA ATGAGAAGTC AAGATCACAT CCAAGTTAGC AAGCAGCACA 
     4141 TTAATAATCA GCAACAGCCA CCTCAACTAC GTTGGAGAAG CAATTCTCTC AATAATGGCC 
     4201 AGCCGAAAAG TACGCGCTGC CAGGCATCTG CCTCCGCGGA GTCATTAAAC TCCCACAGTG 
     4261 GTCACCCCAC TGCTGATGTA CAGACTTTCC AGGCAAAGCG CCATATTCAT CAACACCGTC 
     4321 AGTCTTACTG TAATTATAAC ACTGGAGGTC AGTTAGAGGG CAATGCAGCC ACTTCCTATC 
     4381 AGAAGCAGAC TGACAAACCC AGCCACTGTA GCCAGTTTGT GACACCTCCG CGGATGAGGA 
     4441 GACAGTTCTC AGCACCCAAT CTCAAAGCTG GTCGAGAAAC CACAGTAACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//