LOCUS       RC224847                8373 bp    DNA     circular  SYN 
DEFINITION  NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1
ACCESSION   NM_017970
VERSION     NM_017970.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="NRDE2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGTTC CCAGCCTTTG CGGGGCTTAG TGAGGCTCCC GATGGCGGGA 
     1081 GCTCCAGGAA AGAGTTAGAC TGGCTGAGCA ACCCAAGCTT TTGTGTTGGA TCCATAACGT 
     1141 CCCTGAGCCA ACAAACTGAA GCAGCTCCAG CCCATGTTTC TGAAGGGTTA CCGCTGACAA 
     1201 GGAGTCATCT GAAATCAGAG TCTTCAGATG AAAGTGACAC TAACAAAAAG CTCAAACAAA 
     1261 CAAGTAGAAA AAAGAAGAAA GAGAAAAAGA AAAAAAGGAA GCATCAGCAT CATAAGAAAA 
     1321 CAAAGAGGAA GCATGGGCCG TCGAGTAGCA GCAGGTCTGA GACAGACACC GATTCTGAAA 
     1381 AGGACAAACC TTCCAGAGGC GTTGGAGGCA GTAAAAAGGA ATCTGAGGAA CCGAATCAAG 
     1441 GAAATAATGC TGCAGCTGAT ACTGGACATC GCTTTGTTTG GCTTGAGGAC ATTCAGGCTG 
     1501 TGACGGGAGA AACCTTCAGA ACAGATAAGA AACCAGATCC TGCGAACTGG GAGTACAAGT 
     1561 CTCTCTACCG AGGGGATATA GCAAGATACA AGAGGAAAGG AGACTCCTGC CTTGGCATTA 
     1621 ACCCTAAGAA GCAGTGCATA TCTTGGGAAG GGACTTCCAC AGAGAAGAAG CATTCACGCA 
     1681 AGCAGGTTGA ACGCTATTTT ACTAAGAAGA GTGTGGGATT AATGAACATC GATGGAGTTG 
     1741 CCATTAGCAG TAAAACTGAA CCTCCCTCAT CTGAGCCCAT CTCCTTTATA CCAGTGAAGG 
     1801 ACTTGGAAGA TGCGGCTCCT GTTACAACCT GGTTGAATCC TCTGGGGATT TATGATCAGT 
     1861 CAACCACACA TTGGCTACAA GGACAGGGTC CTCCAGAGCA GGAATCAAAG CAGCCAGACG 
     1921 CACAGCCAGA CAGCGAGAGT GCGGCTCTCA AGGCCAAGGT GGAGGAGTTT AACAGGAGGG 
     1981 TGCGGGAGAA TCCTCGGGAT ACGCAGCTGT GGATGGCATT TGTTGCTTTT CAGGACGAGG 
     2041 TCATGAAAAG TCCTGGCCTG TATGCCATCG AGGAAGGAGA GCAGGAAAAG CGAAAGAGGT 
     2101 CCCTGAAGCT CATTCTGGAG AAGAAGCTGG CCATTCTGGA GCGGGCCATT GAGAGCAACC 
     2161 AGAGCAGTGT GGATCTGAAA CTGGCCAAGC TGAAGCTCTG CACAGAGTTC TGGGAGCCCT 
     2221 CCACTCTGGT CAAAGAGTGG CAGAAACTGA TATTTTTGCA TCCCAATAAT ACAGCCCTTT 
     2281 GGCAGAAATA CCTTTTATTT TGCCAGAGCC AGTTTAGTAC CTTTTCGATA TCAAAAATTC 
     2341 ACAGTCTTTA TGGAAAATGC TTGAGCACTT TGTCTGCTGT TAAGGACGGC AGCATCTTAT 
     2401 CTCACCCTGC GTTGCCTGGC ACGGAAGAGG CCATGTTTGC ACTCTTTCTT CAGCAGTGCC 
     2461 ACTTTCTGCG GCAGGCTGGC CACTCTGAGA AGGCCATCTC ATTGTTCCAG GCCATGGTGG 
     2521 ACTTCACCTT CTTCAAACCC GACAGCGTGA AAGATCTGCC TACCAAAGGA CAGGTGGAAT 
     2581 TCTTTGAACC CTTTTGGGAC AGTGGAGAGC CCCGGGCTGG GGAGAAGGGA GCCCGAGGCT 
     2641 GGAAGGCGTG GATGCACCAG CAGGAACGAG GTGGCTGGGT GGTCATCAAC CCAGATGAGG 
     2701 ATGACGATGA ACCAGAAGAG GATGACCAGG AAATAAAAGA TAAGACTCTG CCCAGGTGGC 
     2761 AGATCTGGCT TGCTGCTGAG CGTTCCCGTG ACCAGAGGCA CTGGCGGCCC TGGCGCCCTG 
     2821 ATAAGACCAA GAAGCAAACC GAGGAAGACT GTGAGGATCC CGAGAGACAG GTGTTGTTTG 
     2881 ATGATATTGG GCAATCTTTG ATCAGACTTT CCAGCCATGA TCTTCAGTTC CAGCTGGTGG 
     2941 AGGCCTTCCT GCAGTTCTTG GGTGTGCCTT CTGGCTTTAC TCCTCCAGCC TCCTGTCTTT 
     3001 ATCTGGCCAT GGATGAGAAC AGCATCTTTG ATAATGGACT TTATGATGAA AAGCCCTTGA 
     3061 CTTTTTTCAA CCCTTTGTTT TCTGGGGCTA GCTGTGTTGG CCGCATGGAT AGGTTGGGCT 
     3121 ATCCTCGCTG GACCAGGGGT CAGAACCGAG AGGGCGAGGA GTTCATCCGC AATGTCTTCC 
     3181 ACCTTGTCAT GCCTTTATTT TCAGGCAAAG AGAAGTCCCA GCTCTGCTTC TCCTGGTTAC 
     3241 AGTATGAGAT TGCAAAGGTC ATTTGGTGCC TGCACACTAA AAACAAGAAG AGATTAAAGT 
     3301 CTCAAGGGAA GAACTGCAAA AAACTAGCCA AGAATCTCCT TAAGGAGCCA GAAAACTGCA 
     3361 ACAACTTTTG CCTGTGGAAG CAGTATGCAC ATCTGGAGTG GTTGCTTGGC AACACGGAGG 
     3421 ATGCCAGAAA AGTTTTTGAC ACAGCACTTG GCATGGCAGG AAGCAGAGAA CTGAAAGACT 
     3481 CTGACCTCTG TGAGCTCAGT CTGCTCTATG CTGAGCTGGA GGTGGAGCTG TCGCCAGAAG 
     3541 TGAGAAGGGC TGCCACAGCT CGAGCTGTTC ACATATTAAC CAAGCTGACT GAGAGCAGCC 
     3601 CCTATGGGCC CTACACTGGA CAGGTGTTGG CTGTTCACAT TTTGAAAGCG CGAAAGGCTT 
     3661 ATGAGCACGC ACTGCAGGAC TGTTTGGGTG ACAGCTGTGT CTCCAATCCA GCTCCCACCG 
     3721 ATTCCTGTAG CCGCCTAATT AGCCTGGCTA AATGCTTCAT GCTCTTCCAG TATTTGACCA 
     3781 TAGGGATTGA TGCTGCTGTG CAGATATACG AACAGGTGTT TGCAAAACTG AACAGTTCTG 
     3841 TTTTCCCAGA AGGCTCTGGC GAGGGGGACA GTGCCAGCTC CCAGAGTTGG ACCAGTGTTC 
     3901 TCGAAGCCAT CACACTGATG CACACGAGCC TGCTGAGATT CCACATGAAA GTGAGTGTTT 
     3961 ACCCGCTGGC CCCTCTGCGA GAGGCACTCT CACAGGCTTT AAAGTTGTAT CCAGGCAACC 
     4021 AGGTTCTTTG GAGGTCCTAT GTACAGATTC AGAATAAGTC CCACAGTGCC AGCAAAACCA 
     4081 GGAGATTTTT TGACACAATC ACCAGGTCTG CCAAACCCTT GGAGCCTTGG TTGTTTGCAA 
     4141 TTGAAGCTGA GAAACTGAGG AAGAGACTGG TGGAAACTGT CCAGAGGTTA GACGGTAGAG 
     4201 AGATCCACGC CACAATTCCT GAGACCGGCT TAATGCATCG GATCCAAGCC CTGTTTGAAA 
     4261 ATGCCATGCG CAGCGACAGT GGCAGCCAGT GCCCCTTGCT GTGGAGGATG TATTTGAACT 
     4321 TTCTGGTTTC CTTAGGAAAT AAAGAAAGAA GCAAAGGTGT ATTCTACAAA GCACTTCAGA 
     4381 ATTGCCCTTG GGCAAAGGTG TTGTACCTGG ACGCCGTGGA GTATTTCCCC GATGAGATGC 
     4441 AGGAGATCCT GGACCTGATG ACTGAGAAGG AGCTCCGGGT GCGCCTGCCG CTGGAGGAGC 
     4501 TGGAGCTGCT GCTGGAGGAT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//